Abstract：
:The mechanism underlying the LTC(4)-induced contraction of guinea-pig taenia coli was determined using the simultaneous measurements of [Ca(2+)](i) and force in whole muscle preparations. Additional experiments were performed in receptor coupled permeabilized preparation. For comparison purposes, the contraction which was induced by a typical G-protein mediated agonist, carbachol was also characterized. LTC(4) induced a contraction in the guinea-pig taenia coli in a concentration-dependent manner. The maximal response was obtained at 100 nM and the EC(50) value was 5.4+/-1.9 nM. Both LTC(4) and carbachol induced increases in [Ca(2+)](i) and force. The maximum force induced by 100 nM LTC(4) was significantly smaller than that induced by 10 microM carbachol, although an increase in [Ca(2+)](i) produced by both agonists was similar. In the permeabilized preparations, carbachol, but not LTC(4), induced an additional force development at a fixed Ca(2+) concentration. LTC(4) induced no increase in [Ca(2+)](i) and force in the Ca(2+)-free solution, while carbachol induced transient increases in both [Ca(2+)](i) and force in a Ca(2+)-free solution. Both diltiazem and SK&F 96365 significantly inhibited the LTC(4)- and carbachol-induced increases in [Ca(2+)](i) and force in normal PSS. The inhibitory pattern of [Ca(2+)](i) by these drugs was also similar. We thus conclude that LTC(4) induces the contraction of the guinea-pig taenia coli mainly through Ca(2+) influx via both the diltiazem-sensitive and SK&F 96365-sensitive Ca(2+) channels, without affecting either the Ca(2+)-sensitivity or the intracellular Ca(2+) release. These results indicated that the mechanism underlying the LTC(4)-induced contraction differs greatly from that for conventional G-protein mediated agonists, such as carbachol.
journal_name
Br J Pharmacoljournal_title
British journal of pharmacologyauthors
Ieiri S,Nishimura J,Hirano K,Suita S,Kanaide Hdoi
10.1038/sj.bjp.0704122subject
Has Abstractpub_date
2001-06-01 00:00:00pages
529-38issue
4eissn
0007-1188issn
1476-5381journal_volume
133pub_type
杂志文章abstract：:In the present study we used a bioassay to study the effects of peroxynitrite (ONOO-) on angiotensin II (A-II)-triggered tension in isolated bovine coronary arteries in order to show the consequences of the previously reported PGI2-synthase inhibition by ONOO- in this model. The following results were obtained: 1. 1 m...
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doi：10.1038/sj.bjp.0702434
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doi：10.1111/j.1476-5381.1986.tb14596.x
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pub_type： 杂志文章,评审
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doi：10.1111/j.1476-5381.1994.tb13209.x
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journal_title：British journal of pharmacology
pub_type： 杂志文章,评审
doi：10.1111/bph.12978
更新日期：2015-04-01 00:00:00
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journal_title：British journal of pharmacology
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doi：10.1111/j.1476-5381.1993.tb13510.x
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doi：10.1111/j.1476-5381.1992.tb12771.x
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journal_title：British journal of pharmacology
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doi：10.1111/j.1476-5381.1994.tb13038.x
更新日期：1994-05-01 00:00:00
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journal_title：British journal of pharmacology
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doi：10.1038/sj.bjp.0704589
更新日期：2002-03-01 00:00:00
