Inactivated recombinant plant virus protects dogs from a lethal challenge with canine parvovirus.


:A vaccine based upon a recombinant plant virus (CPMV-PARVO1), displaying a peptide derived from the VP2 capsid protein of canine parvovirus (CPV), has previously been described. To date, studies with the vaccine have utilized viable plant chimaeric particles (CVPs). In this study, CPMV-PARVO1 was inactivated by UV treatment to remove the possibility of replication of the recombinant plant virus in a plant host after manufacture of the vaccine. We show that the inactivated CVP is able to protect dogs from a lethal challenge with CPV following parenteral immunization with the vaccine. Dogs immunized with the inactivated CPMV-PARVO1 in adjuvant displayed no clinical signs of disease and shedding of CPV in faeces was limited following CPV challenge. All immunized dogs elicited high titres of peptide-specific antibody, which neutralized CPV in vitro. Levels of protection, virus shedding and VP2-specific antibody were comparable to those seen in dogs immunized with the same VP2- peptide coupled to keyhole limpet hemocyanin (KLH). Since plant virus-derived vaccines have the potential for cost-effective manufacture and are not known to replicate in mammalian cells, they represent a viable alternative to current replicating vaccine vectors for development of both human and veterinary vaccines.






Langeveld JP,Brennan FR,Martínez-Torrecuadrada JL,Jones TD,Boshuizen RS,Vela C,Casal JI,Kamstrup S,Dalsgaard K,Meloen RH,Bendig MM,Hamilton WD




Has Abstract


2001-06-14 00:00:00
















  • Pre-vaccine plasma levels of soluble inflammatory indices negatively predict responses to HAV, HBV, and tetanus vaccines in HCV and HIV infection.

    abstract:BACKGROUND:Chronic hepatitis C virus (HCV) and HIV infections are associated with impaired responses to neo-antigens contained in hepatitis A virus (HAV)/hepatitis B virus (HBV) vaccines, yet responsible mechanisms are unclear. METHODS:ACTG 5232 and CFAR0910 were clinical trials where pre-vaccine levels of plasma IP10...


    pub_type: 杂志文章


    authors: Shive CL,Judge CJ,Clagett B,Kalayjian RC,Osborn M,Sherman KE,Fichtenbaum C,Gandhi RT,Kang M,Popkin DL,Sieg SF,Lederman MM,Rodriguez B,Anthony DD

    更新日期:2018-01-25 00:00:00

  • Selectively willing and conditionally able: HIV vaccine trial participation among women at "high risk" of HIV infection.

    abstract::Efficacy studies of investigational HIV vaccines require enrollment of individuals at 'high risk' for HIV. This paper examines participation in HIV vaccine trials among women at 'high risk' for HIV acquisition. In-depth interviews were conducted with 17 African-American women who use crack cocaine and/or exchange sex ...


    pub_type: 杂志文章


    authors: Voytek CD,Jones KT,Metzger DS

    更新日期:2011-08-18 00:00:00

  • Influenza and pneumococcal vaccination and risk of stroke or transient ischaemic attack-matched case control study.

    abstract:BACKGROUND:Evidence that respiratory infections trigger stroke suggests that influenza or pneumococcal vaccination might prevent stroke. We aimed to investigate whether influenza or pneumococcal vaccination or both together were associated with reduced risk of stroke or transient ischaemic attack (TIA). METHODS:We use...


    pub_type: 杂志文章


    authors: Siriwardena AN,Asghar Z,Coupland CC

    更新日期:2014-03-10 00:00:00

  • Proximity to safety-net clinics and HPV vaccine uptake among low-income, ethnic minority girls.

    abstract:PURPOSE:Human Papillomavirus (HPV) vaccine uptake remains low. Although publicly funded programs provide free or low cost vaccines to low-income children, barriers aside from cost may prevent disadvantaged girls from getting vaccinated. Prior studies have shown distance to health care as a potential barrier to utilizin...


    pub_type: 杂志文章


    authors: Tsui J,Singhal R,Rodriguez HP,Gee GC,Glenn BA,Bastani R

    更新日期:2013-04-12 00:00:00

  • Accelerated and long term stability study of Pfs25-EPA conjugates adjuvanted with Alhydrogel®.

    abstract::Pfs25, a Plasmodium falciparum surface protein expressed during zygote and ookinete stages in infected mosquitoes, is a lead transmission-blocking vaccine candidate against falciparum malaria. To enhance immunogenicity, recombinant Pfs25 was chemically conjugated to recombinant nontoxic Pseudomonas aeruginosa ExoProte...


    pub_type: 杂志文章


    authors: Zhu D,Wu Y,McClellan H,Dai W,Rausch K,Jones D,Aebig J,Barnafo E,Butler B,Lambert L,Narum DL,Duffy PE

    更新日期:2017-05-31 00:00:00

  • Influence of glycosylphosphatidylinositol anchorage on the efficacy of DNA vaccines encoding Plasmodium yoelii merozoite surface protein 4/5.

    abstract::Immune responses induced to DNA vaccination vary considerably and depend on a variety of factors, including the physical form in which the antigen is expressed by target cells and presented to the immune system. Data on the effect of these factors will aid improved design of DNA vaccines and facilitate their further d...


    pub_type: 杂志文章


    authors: Wang L,Kedzierski L,Schofield L,Coppel RL

    更新日期:2005-07-14 00:00:00

  • Protective efficacy of a trivalent recombinant hemagglutinin protein vaccine (FluBlok®) against influenza in healthy adults: a randomized, placebo-controlled trial.

    abstract:BACKGROUND:Development of influenza vaccines that do not use embryonated eggs as the substrate for vaccine production is a high priority. We conducted this study to determine the protective efficacy a recombinant, baculovirus-expressed seasonal trivalent influenza virus hemagglutinin (rHA0) vaccine (FluBlok(®)). METHO...


    pub_type: 杂志文章,多中心研究,随机对照试验


    authors: Treanor JJ,El Sahly H,King J,Graham I,Izikson R,Kohberger R,Patriarca P,Cox M

    更新日期:2011-10-13 00:00:00

  • A Semliki Forest virus replicon vectored DNA vaccine expressing the E2 glycoprotein of classical swine fever virus protects pigs from lethal challenge.

    abstract::Classical swine fever virus (CSFV) causes significant losses in pig industry in many countries in Asia and Europe. The E2 glycoprotein of CSFV is the main target for neutralizing antibodies. Recently, the replicon of alphaviruses, such as Semliki Forest virus (SFV), has been developed as replicative expression vectors...


    pub_type: 杂志文章


    authors: Li N,Qiu HJ,Zhao JJ,Li Y,Wang MJ,Lu BW,Han CG,Hou Q,Wang ZH,Gao H,Peng WP,Li GX,Zhu QH,Tong GZ

    更新日期:2007-04-12 00:00:00

  • Construction and characterisation of O139 cholera vaccine candidates.

    abstract::The hemagglutinin/protease (HA/P) seems to be an attractive locus for the insertion of heterologous tags in live cholera vaccine strains. A deltaCTXphi spontaneous mutant derived from a pathogenic strain of O139 Vibrio cholerae was sequentially manipulated to obtain hapA Colon, two colons celA derivatives which were l...


    pub_type: 杂志文章


    authors: Ledón T,Valle E,Valmaseda T,Cedré B,Campos J,Rodríguez BL,Marrero K,García H,García L,Fando R

    更新日期:2003-03-07 00:00:00

  • Early disappearance of maternal anti-measles, mumps, rubella, and varicella antibodies in Indian infants.

    abstract:BACKGROUND:Immunization of children with vaccines against Measles, Mumps, Rubella, and Varicella (MMRV) is practiced globally with varied recommendations. In India, measles vaccine is administered alone or as MMR at 9 months age. Varicella vaccine is not routinely used. Immunization age is a function of disappearance o...


    pub_type: 杂志文章


    authors: Malshe N,Palkar S,Kulkarni R,Lalwani S,Mishra AC,Arankalle V

    更新日期:2019-03-07 00:00:00

  • Plant heat shock protein 90 as carrier-adjuvant for immunization against a reporter antigen.

    abstract::Here, we evaluated the modulation of the immune response induced by Hsp90 of Nicotiana benthamiana (NbHsp90.3) against the Maltose Binding Protein (MBP) as a reporter antigen. Equimolar quantities of recombinant proteins were administered in mice as follows: MBP alone (MBP group), a mixture of MBP and rNbHsp90.3 (MBP+...


    pub_type: 杂志文章


    authors: Corigliano MG,Fenoy I,Sander V,Maglioco A,Goldman A,Clemente M

    更新日期:2013-12-02 00:00:00

  • Expression of pertussis toxin subunit S4 as an intracytoplasmic protein in Bacillus subtilis.

    abstract::The expression and secretion of pertussis toxin subunits S1 to S5 in Bacillus subtilis by the aid of a bacillary signal sequence has been reported. While secretion of subunit S1 was high, that of others was low. Ways have now been explored to improve the yield, using S4 as an example. The addition of a protease inhibi...


    pub_type: 杂志文章


    authors: Himanen JP,Taira S,Sarvas M,Saris P,Runeberg-Nyman K

    更新日期:1990-12-01 00:00:00

  • Vaccination of cattle against B. bovis infection with live attenuated parasites and non-viable immunogens.

    abstract::Susceptible dairy cattle were immunized with attenuated, live calf-derived, in vitro-cultured or biologically cloned Babesia bovis, with non-viable exoantigens, or with recombinant rhoptry-associated protein 1 (rRAP1). Antibody response assessed by the indirect fluorescent assay (IFA) and by the growth inhibition acti...


    pub_type: 临床试验,杂志文章


    authors: Fish L,Leibovich B,Krigel Y,McElwain T,Shkap V

    更新日期:2008-12-19 00:00:00

  • A systematic approach to vaccine complexity using an automaton model of the cellular and humoral immune system. I. Viral characteristics and polarized responses.

    abstract::A modern approach to vaccination faces the compound complexity of microorganism behavior and immune response triggering and regulation. Since computational modeling can yield useful guidelines for biological experimentation, we have used IMMSIM(3), a cellular automaton model for simulating humoral- and cell-mediated r...


    pub_type: 杂志文章


    authors: Kohler B,Puzone R,Seiden PE,Celada F

    更新日期:2000-11-22 00:00:00

  • Is the pneumococcal vaccine Advance Market Commitment motivating innovation and increasing manufacturing capacity? Some preliminary answers.

    abstract::This paper seeks to give some preliminary evidence on the potential outcome of the pneumococcal vaccine Advance Market Commitment (AMC), with a focus on its impact on innovation in 'emerging' vaccine manufacturers in developing countries. The evidence is derived from a series of interviews with executives at industria...


    pub_type: 杂志文章


    authors: Plahte J

    更新日期:2012-03-23 00:00:00

  • Species-specific CD1-restricted innate immunity for the development of HIV vaccine.

    abstract::The human immune defense system is composed of two distinct elements: innate immunity located primarily at body surfaces restricted by species-specific CD1 molecules and acquired immunity found mainly in internal compartments associated with individually restricted MHC molecules. Historically, effective vaccines have ...


    pub_type: 杂志文章,评审


    authors: Takahashi H

    更新日期:2010-05-26 00:00:00

  • Phase I/II studies to evaluate safety and immunogenicity of a recombinant gp350 Epstein-Barr virus vaccine in healthy adults.

    abstract::Two double-blind randomised controlled studies (phase I and I/II) were performed to assess for the first time the safety and immunogenicity of a recombinant subunit gp350 Epstein-Barr virus (EBV) vaccine in 148 healthy adult volunteers. All candidate vaccine formulations had a good safety profile and were well tolerat...


    pub_type: 杂志文章,随机对照试验


    authors: Moutschen M,Léonard P,Sokal EM,Smets F,Haumont M,Mazzu P,Bollen A,Denamur F,Peeters P,Dubin G,Denis M

    更新日期:2007-06-11 00:00:00

  • Multi-epitope DnaK peptide vaccine against S.Typhi: An in silico approach.

    abstract::Salmonella is one of the key global causes of food and water borne enteric infections, responsible for significant morbidity and mortality worldwide especially in developing countries. Currently available vaccines against typhoid are moderately effective with several side effects and not efficacious against all Salmon...


    pub_type: 杂志文章


    authors: Verma S,Sugadev R,Kumar A,Chandna S,Ganju L,Bansal A

    更新日期:2018-06-27 00:00:00

  • Safety, immunogenicity and efficacy of poxvirus-based vector vaccines expressing the haemagglutinin gene of a highly pathogenic H5N1 avian influenza virus in pigs.

    abstract::This study investigates the safety, immunogenicity and efficacy of different pox-vector vaccines expressing the haemagglutinin of a highly pathogenic (HP) H5N1 avian influenza virus (AIV) (A/chicken/Indonesia/7/03) in pigs. Pigs were vaccinated twice, with a 4-week interval, with a fowlpox (TROVAC), a canarypox (ALVAC...


    pub_type: 杂志文章


    authors: Kyriakis CS,De Vleeschauwer A,Barbé F,Bublot M,Van Reeth K

    更新日期:2009-04-06 00:00:00

  • Colostral transmission of BTV-8 antibodies from dairy cows six years after vaccination.

    abstract::Bluetongue virus (BTV) antibodies were analysed in 27 Swiss calves born in 2016 at the age of 16-19 days using competitive enzyme-linked-immunosorbent-assay (cELISA) and virus neutralization test (VNT) (animal trial permission number: 75684). Obligatory documentation proved that 15 of 27 dams were BTV-8 vaccinated onc...


    pub_type: 杂志文章


    authors: Ayrle H,Mevissen M,Kaske M,Vögtlin A,Fricker R,Hoffmann B,Büttner M,Marinovic Z,Walkenhorst M

    更新日期:2018-09-18 00:00:00

  • Characterization of a concealed antigen Hq05 from the hard tick Haemaphysalis qinghaiensis and its effect as a vaccine against tick infestation in sheep.

    abstract::Positive clone Hq05 of Haemaphysalis qinghaiensis (named following those cDNA clones cloned from the tick before) was obtained by differentially screening of a cDNA library of the tick with rabbit anti-tick salivary gland serum and rabbit anti-tick saliva serum. Hq05 contains an open reading frame (ORF) of 540bp that ...


    pub_type: 杂志文章


    authors: Gao J,Luo J,Fan R,Schulte-Spechtel UC,Fingerle V,Guan G,Zhao H,Li Y,Ren Q,Ma M,Liu Z,Liu A,Dang Z,Sugimoto C,Yin H

    更新日期:2009-01-14 00:00:00

  • Efficacy study of a new albumin-free human diploid cell rabies vaccine (Lyssavac-HDC, Berna) in 100 severely rabies-exposed Thai patients.

    abstract::A newly developed human diploid cell rabies vaccine (Lyssavac-HDC), produced without added serum albumin and with an effort to remove the virus-inactivating beta-propriolactone prior to addition of the gelatin, L-cysteine and potassium phosphate stabilizer, was tested for safety immunogenicity, adverse reactions and e...


    pub_type: 临床试验,杂志文章


    authors: Wilde H,Glueck R,Khawplod P,Cryz SJ,Tantawichien T,Thipkong P,Chomchey P,Prakongsri S,Benjavongkulchai M,Sumboonanondha A

    更新日期:1995-04-01 00:00:00

  • Are immunosuppressive medications associated with decreased responses to routine immunizations? A systematic review.

    abstract:BACKGROUND:Long-term immunosuppressive medications are being used more commonly for a variety of medical conditions, including immune-mediated diseases and organ transplantation. While these medications are often necessary, they are associated with an increased risk of serious infections. Vaccination may be a way to pr...


    pub_type: 杂志文章,评审


    authors: Agarwal N,Ollington K,Kaneshiro M,Frenck R,Melmed GY

    更新日期:2012-02-14 00:00:00

  • Effects of a multi-faceted program to increase influenza vaccine uptake among health care workers in nursing homes: A cluster randomised controlled trial.

    abstract::Despite the recommendation of the Dutch association of nursing home physicians (NVVA) to be immunized against influenza, vaccine uptake among HCWs in nursing homes remains unacceptably low. Therefore we conducted a cluster randomised controlled trial among 33 Dutch nursing homes to assess the effects of a systematical...


    pub_type: 杂志文章,随机对照试验


    authors: Looijmans-van den Akker I,van Delden JJ,Verheij TJ,van der Sande MA,van Essen GA,Riphagen-Dalhuisen J,Hulscher ME,Hak E

    更新日期:2010-07-12 00:00:00

  • Comparative immunogenicity analysis of modified vaccinia Ankara vectors expressing native or modified forms of hepatitis C virus E1 and E2 glycoproteins.

    abstract::We have evaluated in C57/Bl6 and HLA-A2.1 transgenic mice the immunogenicity of three MVA vectors expressing either native HCV E1E2 polyprotein, truncated and secreted E1 (E'1(311)) and E2 (E'2(661)) proteins, or a chimeric E1E2 heterodimer presented at the plasma membrane. Immunization induced mainly a Th1 response i...


    pub_type: 杂志文章


    authors: Abraham JD,Himoudi N,Kien F,Berland JL,Codran A,Bartosch B,Baumert T,Paranhos-Baccala G,Schuster C,Inchauspé G,Kieny MP

    更新日期:2004-09-28 00:00:00

  • Dendritic cell (DC) based therapy for cervical cancer: use of DC pulsed with tumour lysate and matured with a novel synthetic clinically non-toxic double stranded RNA analogue poly [I]:poly [C(12)U] (Ampligen R).

    abstract::Human papilloma virus (HPV) found in 99.7% of cervical cancers represents an attractive immunotherapeutic target for novel adjuvant dendritic cell (DC) immunotherapy. DC primed with HPV antigens have been shown to be capable of inducing CTL responses powerful enough to eradicate established murine tumours expressing H...


    pub_type: 杂志文章,评审


    authors: Adams M,Navabi H,Jasani B,Man S,Fiander A,Evans AS,Donninger C,Mason M

    更新日期:2003-01-30 00:00:00

  • Laboratory and field evaluation of Schistosoma japonicum DNA vaccines in sheep and water buffalo in China.

    abstract::Vaccines are needed to control zoonotic Schistosoma japonicum infection and several vaccine candidates have now been identified. Two of these (Sj28GST and Sj23) have shown particular promise in sheep when injected with Freund's adjuvants. The objective of the present work was to find a vaccine formulation which may ha...


    pub_type: 杂志文章


    authors: Shi F,Zhang Y,Ye P,Lin J,Cai Y,Shen W,Bickle QD,Taylor MG

    更新日期:2001-11-12 00:00:00

  • Antibody response to 7-valent conjugated pneumococcal vaccine in patients with chronic lymphocytic leukaemia.

    abstract::Chronic lymphocytic leukaemia (CLL) is a common adulthood mature B-cell neoplasm. Infections are the most important cause of mortality in this condition, and Streptococcus pneumoniae has been considered the most important single pathogen. We investigated the immunogenicity of 7-valent pneumococcal conjugate vaccine in...


    pub_type: 杂志文章


    authors: Sinisalo M,Vilpo J,Itälä M,Väkeväinen M,Taurio J,Aittoniemi J

    更新日期:2007-12-21 00:00:00

  • Nasal vaccination induces the ability to eliminate Candida colonization without influencing the pre-existing antigen-specific IgE Abs: a possibility for the control of Candida-related atopic dermatitis.

    abstract::In some cases of atopic dermatitis (AD), a possible pathological contribution to disease development by Candida albicans (C. albicans) has been suggested. AD patients with severe symptoms showing positive capsulated hydrolic carrier polymer radioallergosorbent test (CAP-RAST) against C. albicans demonstrated significa...


    pub_type: 杂志文章


    authors: Suenobu N,Kweon MN,Kiyono H

    更新日期:2002-07-26 00:00:00

  • Evaluation of potentially achievable vaccination coverage with simultaneous administration of vaccines among children in the United States.

    abstract:BACKGROUND:Routine administration of all age-appropriate doses of vaccines during the same visit is recommended for children by the National Vaccine Advisory Committee (NVAC) and the Advisory Committee on Immunization Practices (ACIP). METHODS:Evaluate the potentially achievable vaccination coverage for ≥4 doses of di...


    pub_type: 杂志文章


    authors: Zhao Z,Smith PJ,Hill HA

    更新日期:2016-06-08 00:00:00