Abstract:
:Selectins are carbohydrate-binding adhesion molecules that play important roles in control of leukocyte traffic. Glycosyltransferases involved in selectin ligand biosynthesis include the alpha1,3-fucosyltransferases FucT-VII and FucT-IV, one or more sialyltransferases, and at least one O-linked branching enzyme. Previous studies have shown that core 2 beta1-6-N-glucosaminyltransferase (C2GlcNAcT-I; EC 2.4.1.102) is required for functional modification of PSGL-1, the leukocyte P-selectin ligand, but have been ambiguous on whether this enzyme is involved in E-selectin ligand formation. Using an attachment and rolling assay under defined shear flow in vitro, this study shows that C2GlcNAcT-I(-) lymphoid cells stably transfected with FucT-VII complementary DNA attach and roll well on E-selectin at 1.5 dynes/cm.(2) Further, attachment and rolling on P-selectin of neutrophils is sharply reduced and that of short- term polarized Th1 cells is virtually abolished, with leukocytes from C2GlcNAcT-I(-/-) mice. In contrast, both neutrophils and Th1 cells from C2GlcNAcT-I(-/-) mice attach and roll as well as wild-type cells on E-selectin. These results show that C2GlcNAcT-I is selectively required for biosynthesis of ligands for P-selectin, but is not essential for at least some E-selectin ligands. Distinct requirements for C2GlcNAcT-I in the formation of ligands for E-selectin versus P-selectin represents a novel level of regulation of expression of selectin ligands and lymphocyte traffic. (Blood. 2001;97:3806-3811)
journal_name
Bloodjournal_title
Bloodauthors
Snapp KR,Heitzig CE,Ellies LG,Marth JD,Kansas GSdoi
10.1182/blood.v97.12.3806subject
Has Abstractpub_date
2001-06-15 00:00:00pages
3806-11issue
12eissn
0006-4971issn
1528-0020journal_volume
97pub_type
杂志文章相关文献
BLOOD文献大全abstract::Chronic granulomatous disease (CGD) is characterized by the absence of a respiratory burst in activated phagocytes. Defects in at least four different genes lead to CGD. Patients with the X-linked form of CGD have mutations in the gene for the beta-subunit of cytochrome b558 (gp91-phox). We studied the molecular defec...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1992-09-15 00:00:00
abstract::The presence and physiologic role of cyclic GMP-dependent protein kinase (G-kinase) in human neutrophils was investigated by Western blot analysis and immunocytochemistry. Small quantities of G-kinase were found in the cytoskeletal-enriched fraction of neutrophil lysates as detected by Western blots using a polyclonal...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1990-08-01 00:00:00
abstract::The tyrosine kinase receptor fetal liver kinase 1 (Flk-1) plays a crucial role in vasculogenesis and angiogenesis, but its target genes remain elusive. Comparing Flk-1(+/+) with Flk-1(-/-) embryonic stem (ES) cells, we identified transcripts regulated by the vascular endothelial growth factor A (VEGF-A)/Flk-1 pathway ...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2003-11-3827
更新日期:2004-06-15 00:00:00
abstract::Although thrombin is a key enzyme in the coagulation cascade and is required for both normal hemostasis and pathologic thrombogenesis, it also participates in its own negative feedback via activation of protein C, which downregulates thrombin generation by enzymatically inactivating factors Va and VIIIa. Our group and...
journal_title:Blood
pub_type: 杂志文章,随机对照试验
doi:10.1182/blood.2019002771
更新日期:2020-02-27 00:00:00
abstract::Human natural killer (NK) cell development is a step-by-step process characterized by phenotypically identified stages. CD161 is a marker informative of the NK cell lineage commitment, whereas CD56, CD117, and CD94/NKG2A contribute to define discrete differentiation stages. In cells undergoing in vitro differentiation...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2011-09-379693
更新日期:2012-04-26 00:00:00
abstract::Phosphatidylserine (PS) is normally confined to the cytoplasmic leaflet of the red blood cell (RBC) membrane, but some sickle RBCs expose PS in the outer leaflet (PS+ cells). This study examined the relationships among PS externalization, fetal hemoglobin content, hydration state, and cell age. Sickle RBCs exhibit a w...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2002-11-3416
更新日期:2003-07-01 00:00:00
abstract::Ectopic expression of fibroblast growth factor receptor 3 (FGFR3) associated with t(4;14) has been implicated in the pathogenesis of human multiple myeloma. Some t(4;14) patients have activating mutations of FGFR3, of which a minority are K650E (thanatophoric dysplasia type II [TDII]). To investigate the role of autop...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2004-09-3686
更新日期:2005-07-01 00:00:00
abstract::Evidence has been provided recently that shows that high concentrations of cytokines can fulfill functions previously attributed to stromal cells, such as promote the survival of, and led to a net increase in human primitive progenitors initiating long-term cultures in vitro (LTC-IC) or engrafting NOD-SCID (nonobese d...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1999-07-15 00:00:00
abstract::To characterize juvenile chronic myelogenous leukemia (JCML), the proliferative properties of bone marrow (BM) and peripheral blood (PB) cells from nine patients were studied using assays for CFU-C and CFU-GEMM and liquid cultures. All specimens showed two reproducible abnormalities: impaired growth of normal hematopo...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1986-05-01 00:00:00
abstract::In this issue of Blood, Vérollet et al show that expression of the HIV-1–derived protein Nef alters the migratory mode adopted by macrophages, enhancing macrophage tissue infiltration and explaining the observed accumulation of tissue-resident macrophages in some HIV-infected patients. ...
journal_title:Blood
pub_type: 评论,杂志文章
doi:10.1182/blood-2015-01-618983
更新日期:2015-03-05 00:00:00
abstract::The capacity to convert exogenous leukotriene A4 to lipoxins (LXs) was investigated in platelet suspensions from patients with myeloproliferative disorders (MPD) (n = 22) and healthy control subjects (n = 14). Platelets isolated from the controls produced mainly LXA4, but also 6(S)-LXA4 and the all-trans isomers of li...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1991-12-01 00:00:00
abstract::Atypical hemolytic-uremic syndrome (aHUS) is associated with genetic complement abnormalities/anti-complement factor H antibodies, which paved the way to treatment with eculizumab. We studied 44 aHUS patients and their relatives to (1) test new assays of complement activation, (2) verify whether such abnormality occur...
journal_title:Blood
pub_type: 临床试验,杂志文章
doi:10.1182/blood-2014-02-558296
更新日期:2014-09-11 00:00:00
abstract::In this study, we randomly compared high doses of the tyrosine kinase inhibitor imatinib combined with reduced-intensity chemotherapy (arm A) to standard imatinib/hyperCVAD (cyclophosphamide/vincristine/doxorubicin/dexamethasone) therapy (arm B) in 268 adults (median age, 47 years) with Philadelphia chromosome-positiv...
journal_title:Blood
pub_type: 杂志文章,随机对照试验
doi:10.1182/blood-2015-02-627935
更新日期:2015-06-11 00:00:00
abstract::The technique described in the preceding paper was applied to 12 abnormal sera selected for their increase in one or more B12-binding proteins. Even in the presence of large amounts of R-type binder, the ammonium sulfate technique gave a reliable separation of R binding proteins from TC II. Measurement of the TC II in...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1975-02-01 00:00:00
abstract::In the maternal decidua, natural killer (NK) cells, characterized by lack of CD16, are found in direct contact with the fetal extravillous trophoblasts (EVTs). It is yet unknown which factors contribute to the specific homing of this unique NK subset to the decidua. In this study we analyze the chemokine receptor repe...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2003-02-0517
更新日期:2003-09-01 00:00:00
abstract::Cultured endothelial cells produce an extracellular matrix (ECM) to which platelets adhere and spread, ultimately resulting in platelet aggregation, thromboxane B2 production, and serotonin release. We have investigated the role of fibrinogen binding to the platelet GPIIb/IIIa complex in these reactions by comparing n...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1985-06-01 00:00:00
abstract::Diffuse large B-cell lymphoma (DLBCL) is a heterogeneous disease defined by transcriptional classifications, specific signaling and survival pathways, and multiple low-frequency genetic alterations. Preclinical model systems that capture the genetic and functional heterogeneity of DLBCL are urgently needed. Here, we g...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2015-09-672352
更新日期:2016-05-05 00:00:00
abstract::Mutations in the TET2 gene are frequent in myeloid disease, although their biologic and prognostic significance remains unclear. We analyzed 355 patients with myelodysplastic syndromes using "next-generation" sequencing for TET2 aberrations, 91 of whom were also subjected to single-nucleotide polymorphism 6.0 array ka...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2010-03-274704
更新日期:2010-11-11 00:00:00
abstract::Treatment with warfarin using a target International Normalized Ratio (INR) range of 1.7 to 2.5 is efficacious for many clinical indications, but the minimal intensity of anticoagulation required for antithrombotic protection has yet to be determined. To evaluate whether patients could be reliably monitored with a les...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1992-04-15 00:00:00
abstract::A hamster antimouse CD3 monoclonal antibody (MoAb) opened the way to experimental studies on the suppression of allograft rejection and cytokine-related morbidity after treatment with antibodies modulating the CD3/T-cell receptor complex (CD3/TCR). Because earlier attempts to suppress graft-versus-host disease (GVHD) ...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1992-11-15 00:00:00
abstract::Mantle cell lymphoma (MCL) is genetically characterized by the t(11;14)(q13;q32) translocation and a high number of secondary chromosomal alterations. However, only a limited number of target genes have been identified. We have studied 10 MCL cell lines and 28 primary tumors with a combination of a high-density single...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2008-07-170183
更新日期:2009-03-26 00:00:00
abstract::The TEL-JAK2 gene fusion, which has been identified in human leukemia, encodes a chimeric protein endowed with constitutive tyrosine kinase activity. TEL-JAK2 transgenic expression in the mouse lymphoid lineage results in fatal and rapid T-cell leukemia/lymphoma. In the present report we show that T-cell leukemic cell...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2006-09-048801
更新日期:2007-05-01 00:00:00
abstract::Sixteen patients with adult T-cell leukemia/lymphoma (ATL) who were all over 50 years of age underwent allogeneic stem cell transplantation with reduced-conditioning intensity (RIST) from HLA-matched sibling donors after a conditioning regimen consisting of fludarabine (180 mg/m2), busulfan (8 mg/kg), and rabbit antit...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2004-11-4193
更新日期:2005-05-15 00:00:00
abstract::The risk of development of CNS leukemia was investigated in 153 adults with acute lymphocytic leukemia (ALL) who received systemic combination chemotherapy without CNS prophylaxis. Overall, 31 patients (20%) developed CNS leukemia after a median of 6 months of therapy; the estimated 1-year incidence of CNS leukemia wa...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1988-11-01 00:00:00
abstract::The B-cell leukemia/lymphoma-2 (BCL-2) family of proteins governs the intrinsic pathway of mitochondrial apoptosis. Dysregulation of BCL-2 has long been known to be a crucial part of the pathophysiology of B-cell lymphomas; however, several early attempts to target this pathway therapeutically were unsuccessful becaus...
journal_title:Blood
pub_type: 杂志文章,评审
doi:10.1182/blood-2017-04-737338
更新日期:2017-08-31 00:00:00
abstract::The effect of heterologous anti-human platelet antibody on human platelet function was examined in the presence and absence of whole plasma as an in vitro model for antibody-induced immune damage to cells. Heterologous IgG anti-human platelet antibody mediated platelet aggregation and released serotonin from both plat...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1976-07-01 00:00:00
abstract::The effects of selectively depleting CD8+ cells from donor bone marrow were assessed in 36 patients receiving transplantation from an HLA-identical sibling as treatment for leukemia. Donor bone marrow underwent ex vivo treatment using anti-Leu-2 monoclonal antibody and complement. Patients received cyclosporine post-t...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1990-07-15 00:00:00
abstract::Although infants with acute lymphoblastic leukemia (ALL) and a germline MLL gene have a better prognosis than comparable infants with a rearranged MLL gene, their optimal therapy is controversial. In 2 consecutive studies, conducted between 1996 and 2002, we treated 22 cases of infant ALL with germline MLL using chemo...
journal_title:Blood
pub_type: 临床试验,杂志文章,多中心研究
doi:10.1182/blood-2005-11-4728
更新日期:2006-06-15 00:00:00
abstract::Shear-induced platelet aggregation (SIPA) involves the sequential interaction of von Willebrand factor (VWF) with both glycoprotein Ib (GPIb) and alphaIIbbeta3 receptors. Type 2B recombinant VWF (2B-rVWF), characterized by an increased affinity for GPIb, induces strong SIPA at a high shear rate (4000 s-1). Despite the...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2002-06-1879
更新日期:2003-06-01 00:00:00
abstract::Duchenne muscular dystrophy (DMD) is caused by mutations in the dystrophin gene on the X-chromosome that result in skeletal and cardiac muscle damage and premature death. Studies in mice, including the mdx mouse model of DMD, have demonstrated that circulating bone marrow-derived cells can participate in skeletal musc...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2004-06-2247
更新日期:2004-12-15 00:00:00