Abstract:
UNLABELLED:Prolonged immobilization of severely ill, bed-ridden patients results in formation of pressure ulcers following inappropriate tissue blood perfusion, and local activation of leukocyte - endothelial cell interactions (L/EC). Various treatment modalities were implemented to improve local microcirculation with controversial results. The aim of our study was to investigate the influence of heparin and buflomedil, drugs improving the microcirculation, on local skin blood perfusion using intravital fluorescent microscopy (IVM) technique. MATERIAL AND METHODS:Experiments were carried out on 24 male hairless mice under inhalatory isofluran anaesthesia. Intravenous injection of FITC-dextran (150 kD, 5%) allowed to visualize capillaries during IVM, after immobilisation of hind limb, in an observation chamber. Observations were performed after i.v. injection of heparin (66 IU/kg b.w., n = 8) or buflomedil (6 mg/kg b.w., n = 8) 30, 120 and 210 minutes after chamber installation. Observations were recorded in 30 sec sequences on S-VHS tapes and evaluated using special software. Functional capillary density (FCD), defined as a total length of red cells perfused capillaries per observation field (expressed in cm/cm(2)), postcapillary venule diameters, and number of sticking leukocytes per 0.2 mm vessel length during 30 sec observation time, served as parameters of skin blood perfusion and activation of L/EC interactions. RESULTS:A statistically significant decrease of FCD from 152.7 +/- 38.5 to 100.7 +/- 36.7 cm/cm (2) (p<0.05) was observed in control animals during 210 min lasting observation. Administration of heparin prevented decrease in FCD occurring in control animals during intravital microscopy, whereas buflomedil was found ineffective. Both drugs induced a nonsignificant reduction in the number of sticking leukocytes, whereas no changes in postcapillary venule diameters could be observed. CONCLUSION:The results suggest a protective effect of heparin in clinical therapeutic doses against impairment of skin perfusion during IVM. This observation may justify a trial on the effects of heparin in prevention of development of pressure ulcers.
journal_name
Eur J Med Resjournal_title
European journal of medical researchauthors
Szczesny G,Veihelmann A,Nolte D,Olszewski WL,Messmer Ksubject
Has Abstractpub_date
2001-04-20 00:00:00pages
175-80issue
4eissn
0949-2321issn
2047-783Xjournal_volume
6pub_type
杂志文章abstract::Cancer and cardiovascular diseases are the main causes for morbidity and mortality in modern society. In the United States of America (USA), over 1.7 million new cancer cases will presumably be observed in 2018. Progress in cancer treatment has greatly improved survival and it is estimated that 15.5 million cancer sur...
journal_title:European journal of medical research
pub_type: 社论
doi:10.1186/s40001-018-0359-0
更新日期:2019-01-03 00:00:00
abstract::Retinyl palmitate and its metabolites retinol and retinoic acid control growth and epithelial differentiation. Systemic or local vitamin A deficiency induced by malnutrition, continuous chemical irritation or locally induced by inflammation causes squamous metaplastic changes in the epithelium of mucous membranes. We ...
journal_title:European journal of medical research
pub_type: 杂志文章
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abstract:BACKGROUND:In subtotal central retinal artery occlusion (CRAO), distinct retinal edema with acute onset irreversible visual loss is common. - METHODS:Clinical observation, fluorescein angiogram. - PATIENT:A 69-year-old patient presented with acute visual loss of his right eye. Risk factors for CRAO were arterial hype...
journal_title:European journal of medical research
pub_type: 杂志文章
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更新日期:2006-01-31 00:00:00
abstract:BACKGROUND:There are many approved patient-related outcome measurement tools regarding ankle pathologies. However, there is none incorporating the range of motion (ROM) as an objective parameter. Most instruments focus on subjective parameters such as pain and impairment at work or daily living. Furthermore, the majori...
journal_title:European journal of medical research
pub_type: 杂志文章
doi:10.1186/s40001-018-0344-7
更新日期:2018-09-28 00:00:00
abstract::CCR5 receptor inhibitors are currently being introduced into clinical practice. In some instances treatment failure is related to the selection of pre-existing CXCR4-tropic minority virus strains. Up to date it is unclear whether the outgrowth of a CXCR4 using reservoir is associated with accelerated HIV-disease. In a...
journal_title:European journal of medical research
pub_type: 杂志文章,评审
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更新日期:2007-10-15 00:00:00
abstract::Invasive aspergillosis and disseminated candidiasis are the two major manifestations of opportunistic invasive mycoses. Their incidence has risen considerably during the past decades, due to more intensive anticancer chemotherapy, organ transplantations, intensive care, and aggressive surgical interventions. Especiall...
journal_title:European journal of medical research
pub_type: 杂志文章,评审
doi:
更新日期:2002-05-31 00:00:00
abstract:BACKGROUND:Sonography has become the imaging technique of choice for guiding intraoperative interventions in abdominal surgery. Due to artefacts from residual air content, however, videothoracoscopic and open intraoperative ultrasound-guided thermoablation of lung malignancies are impossible. Lung flooding is a new met...
journal_title:European journal of medical research
pub_type: 杂志文章
doi:10.1186/2047-783X-18-23
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journal_title:European journal of medical research
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abstract::Determination of fecal excretion of the serum proteinase inhibitor alpha(1)-antitrypsin (AAT) is established for quantification of intestinal protein loss. It was demonstrated to be increased both in quiescent and in active inflammatory bowel diseases (IBD, Crohn's disease and ulcerative colitis). The (patho)physiolog...
journal_title:European journal of medical research
pub_type: 杂志文章,评审
doi:
更新日期:1998-02-21 00:00:00
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journal_title:European journal of medical research
pub_type: 杂志文章
doi:
更新日期:2000-04-19 00:00:00
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journal_title:European journal of medical research
pub_type: 杂志文章
doi:10.1186/s40001-017-0300-y
更新日期:2018-01-08 00:00:00
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journal_title:European journal of medical research
pub_type: 杂志文章
doi:
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abstract::Triple A (four A; Allgrove syndrome) syndrome is a an autosomal recessive disorder characterized by alacrimia, achalasia, adrenocortical insufficiency, and various neurological abnormalities. We report a case of triple A syndrome in a 14-year-old female patient together with oral manifestations. The importance of this...
journal_title:European journal of medical research
pub_type: 杂志文章
doi:
更新日期:2003-07-31 00:00:00
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journal_title:European journal of medical research
pub_type: 杂志文章
doi:10.1186/2047-783X-18-15
更新日期:2013-06-03 00:00:00
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journal_title:European journal of medical research
pub_type: 临床试验,杂志文章
doi:
更新日期:1999-02-25 00:00:00
abstract::Soot FR 101, Printex 90 and Chrysotile B are frequently found in indoor air pollutants phagocytized by alveolar macrophages (AM) involved in inflammatory pulmonary processes as, e.g. in cytokine secretions. The transcription factor NF-kappaB has a role in the trans-duction pathway of proinflammatory cytokines like IL-...
journal_title:European journal of medical research
pub_type: 杂志文章
doi:
更新日期:1998-09-17 00:00:00
abstract::A 65-year old woman with known history of reactive depression and failed suicide attempts ingested 7 mg digitoxin at 09.00 h. After vomiting 4 hours later, she reported the drug intake to her husband who thereupon summoned a physician. Arriving at 16.00 h, the physician was informed about the suicide attempt, but fail...
journal_title:European journal of medical research
pub_type: 杂志文章
doi:
更新日期:1996-11-25 00:00:00
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journal_title:European journal of medical research
pub_type: 临床试验,杂志文章,随机对照试验
doi:
更新日期:2006-08-30 00:00:00
abstract::We report the case of a 64-year old woman with severe attacks of allergic alveolitis occurring frequently independent of a concrete place. These attacks were regularely preceded by diarrhoe. Laboratory data showed marked leukopenia and circulating Candida albicans (C.a.)-antigen. Bronchoalveolar lavage revealed an inc...
journal_title:European journal of medical research
pub_type: 杂志文章
doi:
更新日期:2000-03-27 00:00:00
abstract::The aim of the study was to investigate whether cholecystokinin, neurotensin, and cholinergic mechanisms act as mediators of bile salt-stimulated exocrine pancreatic secretion. Ten fasting healthy subjects provided with a double-lumen tube received 2, 4, and 6 g cattle bile and 200, 400, and 600 mg Na-taurodeoxycholat...
journal_title:European journal of medical research
pub_type: 杂志文章
doi:
更新日期:1997-01-01 00:00:00
abstract::Plexiform neurofibroma (PN) is a rare benign tumor and a special subtype of neurofibromatosis type 1 (NF1). Though the incidence is low, giant PN of the craniomaxillofacial region could result in severe hemifacial hypertrophy which is known as a typical manifestation of NF1 in young children. Here, we retrospectively ...
journal_title:European journal of medical research
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doi:10.1186/2047-783x-15-2-84
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journal_title:European journal of medical research
pub_type: 临床试验,杂志文章,随机对照试验
doi:
更新日期:2002-04-30 00:00:00
abstract::Previous studies on Langerhans'-cell histiocytosis (LCH) were mostly retrospective and patients had been recruited over a long time period. The aim of the present study was to establish data on the clinical presentation, diagnosis and treatment of currently affected patients. Data on the clinical history, diagnostic w...
journal_title:European journal of medical research
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更新日期:2004-11-29 00:00:00
abstract::Experiments in space have shown that T lymphocyte function is altered in more than 50% of space crew members. There is strong evidence that such effect is due to stress rather than to weightlessness per se. However the health of astronauts was never threatened so far. Experiments in-vitro with cultures of human periph...
journal_title:European journal of medical research
pub_type: 杂志文章
doi:
更新日期:1999-09-09 00:00:00
abstract::Antibiotic-resistant organisms causing both hospital- and community-acquired complicated skin and soft-tissue infections (cSSTI) are increasingly reported. A substantial medical and economical burden associated with MRSA colonisation or infection has been documented. The number of currently available appropriate antim...
journal_title:European journal of medical research
pub_type: 杂志文章,评审
doi:10.1186/2047-783x-15-12-554
更新日期:2010-11-30 00:00:00
abstract:OBJECTIVE:The influence of cytomechanical forces in cellular migration, proliferation and differentiation of mesenchymal stem cells (MSCs) is still poorly understood in detail. METHODS:Human MSCs were isolated and cultivated onto the surface of a 3 x 3 mm porcine collagen I / III carrier. After incubation, cell cultur...
journal_title:European journal of medical research
pub_type: 杂志文章
doi:10.1186/2047-783x-15-1-35
更新日期:2010-01-29 00:00:00
abstract:BACKGROUND:Electroacupuncture is well known for its advantageous neuroanalgesic and therapeutic effects on myocardial ischemia-reperfusion injury. The purpose of the present research was to verify whether electroacupuncture can alleviate bupivacaine-induced myocardial injury. METHODS:Specific pathogen-free Wistar rats...
journal_title:European journal of medical research
pub_type: 杂志文章
doi:10.1186/s40001-020-00431-4
更新日期:2020-08-01 00:00:00
abstract::Drug development in the field of HIV treatment is rapid. New nucleoside analogues (NRTI), non-nucleoside analogue reverse transcriptase inhibitors (NNRTI), and protease inhibitors (PI) are currently being investigated in human trials. Furthermore, inhibitors of HIV attachment, fusion and integrase with novel modes of ...
journal_title:European journal of medical research
pub_type: 杂志文章,评审
doi:
更新日期:2007-10-15 00:00:00
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journal_title:European journal of medical research
pub_type: 杂志文章
doi:
更新日期:2008-01-23 00:00:00
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journal_title:European journal of medical research
pub_type: 杂志文章,评审
doi:10.1186/s40001-017-0295-4
更新日期:2017-12-02 00:00:00