Human skin-derived mast cells can proliferate while retaining their characteristic functional and protease phenotypes.

Abstract:

:Human mast cells in adult tissues have been thought to have limited, if any, proliferative potential. The current study examined mast cells obtained from adult skin and cultured in serum-free medium with recombinant human stem cell factor. During the first 4 weeks of culture, the percentages of mast cells increased from 10 to almost 100. After 8 weeks, a 150-fold increase in the number of mast cells was observed. When freshly dispersed mast cells were individually sorted onto human fibroblast monolayers and cultured for 3 weeks, one or more mast cells were detected in about two thirds of the wells, and in about two thirds of these wells the surviving mast cells showed evidence of proliferation, indicating most mast cells in skin can proliferate. Such mast cells all expressed high surface levels of Kit and Fc epsilon RI, each of which were functional, indicating IgE was not required for Fc epsilon RI expression on mast cells. Such mast cells also retained the MC(TC) protease phenotype of mast cells that normally reside in the dermis. After 4 to 8 weeks of culture these mast cells degranulated in response to substance P and compound 48/80, characteristics of skin-derived mast cells that persist outside of the cutaneous microenvironment. (Blood. 2001;97:2045-2052)

journal_name

Blood

journal_title

Blood

authors

Kambe N,Kambe M,Kochan JP,Schwartz LB

doi

10.1182/blood.v97.7.2045

subject

Has Abstract

pub_date

2001-04-01 00:00:00

pages

2045-52

issue

7

eissn

0006-4971

issn

1528-0020

journal_volume

97

pub_type

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