Autoantibodies against neonatal heart M1 muscarinic acetylcholine receptor in children with congenital heart block.

Abstract:

:Isolated congenital heart block may be associated with autoimmune disorder such as Sjögren Syndrome and systemic lupus erythematosus. In this work we demonstrate circulating autoantibodies against neonatal heart M1 muscarinic acetylcholine receptor (mAChR) in the sera of children with congenital heart block. This antibody were able to react with the second extracellular loop of the human M1 mAChR as demonstrated using a synthetic peptide in enzyme immune assay and binding assay. Affinity purified anti-peptide IgG as well as total IgG from children with congenital heart block, interfered with the specific radioligand occupancy from neonatal heart M1 mAChR, interacting irreversibly. The antipeptide antibodies also displayed an 'agonist-like' activity, i.e. decreased contractility, activated nitric oxide synthase activity and increased production of cyclic GMP. All of these effects were selectively blunted by pirenzepine and neutralized by the synthetic M1 peptide. Both binding and biological effects were obtained using neonatal rat heart instead adult heart and were independent of Ro/SS-A and La/SS-B antibodies and were also absent in the sera of normal children. A clinical relevance of these findings is demonstrated by a strong association between the existence of circulating M1 mAChR antipeptide antibodies and the presence of isolated congenital heart block, making these antibodies a proper marker of this disease.

journal_name

J Autoimmun

journal_title

Journal of autoimmunity

authors

Borda E,Sterin-Borda L

doi

10.1006/jaut.2000.0461

subject

Has Abstract

pub_date

2001-03-01 00:00:00

pages

143-50

issue

2

eissn

0896-8411

issn

1095-9157

pii

S0896-8411(00)90461-X

journal_volume

16

pub_type

杂志文章
  • Autoantibody explosion in antiphospholipid syndrome.

    abstract::Antiphospholipid syndrome (APS) is characterized by thrombosis and pregnancy loss in the presence of antiphospholipid antibodies (aPL), mainly anticardiolipin, anti-beta2-glycoprotein I and lupus anticoagulant. However, similar to systemic lupus erythematosus, APS is also characterized by multiple other autoantibodies...

    journal_title:Journal of autoimmunity

    pub_type: 杂志文章

    doi:10.1016/j.jaut.2007.11.011

    authors: Shoenfeld Y,Twig G,Katz U,Sherer Y

    更新日期:2008-02-01 00:00:00

  • Multi-peptide coupled-cell tolerance ameliorates ongoing relapsing EAE associated with multiple pathogenic autoreactivities.

    abstract::The probability that epitope spreading occurs in multiple sclerosis (MS) and the fact that patients have been shown to respond to multiple myelin epitopes concurrently makes the use of peptide-specific tolerance therapies targeting single epitopes problematic. To attempt to overcome this limitation, we have employed c...

    journal_title:Journal of autoimmunity

    pub_type: 杂志文章

    doi:10.1016/j.jaut.2006.12.002

    authors: Smith CE,Miller SD

    更新日期:2006-12-01 00:00:00

  • The role of NK cell activity in the pathogenesis of poly I:C accelerated and spontaneous diabetes in the diabetes prone BB rat.

    abstract::The development of insulin dependent diabetes mellitus (IDDM) and diabetes in the diabetes prone (DP) BB rat animal model of IDDM is thought to be due to an autoimmune process. Natural killer (NK) cells have been implicated but not proven to play a pathogenetic role in BB rats due to the increased NK cell number and a...

    journal_title:Journal of autoimmunity

    pub_type: 杂志文章

    doi:10.1016/s0896-8411(95)80021-2

    authors: Sobel DO,Azumi N,Creswell K,Holterman D,Blair OC,Bellanti JA,Abbassi V,Hiserodt JC

    更新日期:1995-12-01 00:00:00

  • Post-thymic tolerance to self antigens.

    abstract::There is now convincing evidence for the imposition of self-tolerance by means of the clonal deletion of self-reactive T cells operating within the thymus. Since not all self components may be encountered there, the question must be asked whether tolerance can occur post-thymically. To test this, we have used transgen...

    journal_title:Journal of autoimmunity

    pub_type: 杂志文章,评审

    doi:10.1016/0896-8411(92)90016-j

    authors: Miller JF

    更新日期:1992-04-01 00:00:00

  • Hu antigen specificities of ANNA-I autoantibodies in paraneoplastic neurological disease.

    abstract::Despite a broad clinical spectrum, paraneoplastic enecephalomyelitis/sensory neuronopathy (PEM/SSN) is characterized by the presence of a common autoantibody, referred to as anti-Hu or type I anti-neuronal nuclear antibody (ANNA-1). The target of these antibodies is a family of four Hu antigens: three (Hel-N1, HuC, Hu...

    journal_title:Journal of autoimmunity

    pub_type: 杂志文章

    doi:10.1006/jaut.1999.0337

    authors: King PH,Redden D,Palmgren JS,Nabors LB,Lennon VA

    更新日期:1999-12-01 00:00:00

  • Sjögren's syndrome at the crossroad of polyautoimmunity.

    abstract::The coexistence of autoimmune diseases (i.e., polyautoimmunity) in Sjögren's syndrome (SS) was investigated in a cross-sectional study involving 410 patients. Logistic regression analysis and the Rogers and Tanimoto index were used to evaluate risk factors and clustering, respectively. There were 134 (32.6%) patients ...

    journal_title:Journal of autoimmunity

    pub_type: 杂志文章

    doi:10.1016/j.jaut.2012.05.008

    authors: Amador-Patarroyo MJ,Arbelaez JG,Mantilla RD,Rodriguez-Rodriguez A,Cárdenas-Roldán J,Pineda-Tamayo R,Guarin MR,Kleine LL,Rojas-Villarraga A,Anaya JM

    更新日期:2012-09-01 00:00:00

  • Reactivities to the Sm autoantigenic complex and the synthetic SmD1-aa83-119 peptide in systemic lupus erythematosus and other autoimmune diseases.

    abstract::The Sm antigenic complex is, besides dsDNA, the most important and specific autoimmune target in systemic lupus erythematosus (SLE). The population of anti-Sm Ab elicited is very heterogeneous in terms of epitope specificity resulting in a strong assay dependent detectability. Based on the description of a new autoant...

    journal_title:Journal of autoimmunity

    pub_type: 杂志文章

    doi:10.1006/jaut.2001.0545

    authors: Jaekel HP,Klopsch T,Benkenstein B,Grobe N,Baldauf A,Schoessler W,Werle E

    更新日期:2001-12-01 00:00:00

  • Differential activities of immunogenic collagen type II peptides in the induction of nasal tolerance to collagen-induced arthritis.

    abstract::Nasal tolerance has recently been used to modulate immune responses in animal models of autoimmunity. We have compared immunogenic collagen type II (CII) peptides for induction of nasal tolerance in DBA/1 mice to collagen-induced arthritis (CIA). Three synthetic peptides corresponding to T cell-stimulating sequences o...

    journal_title:Journal of autoimmunity

    pub_type: 杂志文章

    doi:10.1006/jaut.1998.0255

    authors: Chu CQ,Londei M

    更新日期:1999-02-01 00:00:00

  • Monocytes contribute to DNA sensing through the TBK1 signaling pathway in type 1 diabetes patients.

    abstract:BACKGROUND:The aberrant recognition of self-nucleic acids by the innate immune system contributes to the pathology of several autoimmune diseases. Although microbial DNA and, in certain instances, self-DNA that is released from damaged cells are primarily recognized by Toll-like receptor 9 (TLR9), recent evidence sugge...

    journal_title:Journal of autoimmunity

    pub_type: 杂志文章

    doi:10.1016/j.jaut.2019.06.005

    authors: Zentsova I,Parackova Z,Kayserova J,Palova-Jelinkova L,Vrabcova P,Volfova N,Sumnik Z,Pruhova S,Petruzelkova L,Sediva A

    更新日期:2019-12-01 00:00:00

  • Phosphorylation abnormalities: NZB mice exhibit a B-cell signalling defect.

    abstract::NZB mice demonstrate common and consistent abnormalities in B-cell activation and signalling. One of the hallmark characteristics of lupus disease is the prevalent hypergammaglobulinaemia, composed primarily of anti-nuclear antibodies. In addition to the hyperproliferation seen in mice exhibiting disease, the B cells ...

    journal_title:Journal of autoimmunity

    pub_type: 杂志文章

    doi:10.1006/jaut.2002.0607

    authors: Tuscano JM,Hsu TC,McKnight H,Ansari AA,Gershwin ME

    更新日期:2002-11-01 00:00:00

  • Autoantibodies with enzymatic properties in human autoimmune diseases.

    abstract::Immunoglobulins (Ig) or antibodies are heavy plasma proteins, with sugar chains added to amino acid residues by N-linked glycosylation and occasionally by O-linked glycosylation. The versatility of antibodies is demonstrated by the various functions that they mediate such as neutralization, agglutination, fixation wit...

    journal_title:Journal of autoimmunity

    pub_type: 杂志文章,评审

    doi:10.1016/j.jaut.2011.05.007

    authors: Wootla B,Lacroix-Desmazes S,Warrington AE,Bieber AJ,Kaveri SV,Rodriguez M

    更新日期:2011-09-01 00:00:00

  • Accessibility of epitopes on the 52-kD Ro/SSA protein (Ro52) and on the RoRNP associated Ro52 protein as determined by anti-peptide antibodies.

    abstract::The Ro ribonucleoprotein particle (RoRNP) is the target of a variety of specific anti-protein autoantibodies produced by patients with systemic autoimmune diseases. RoRNPs, the function of which remains elusive, appear to be rather heterogeneous in terms of their molecular composition. Among several Ro proteins, a pro...

    journal_title:Journal of autoimmunity

    pub_type: 杂志文章

    doi:10.1006/jaut.1996.0122

    authors: Ricchiuti V,Pruijn GJ,Thijssen JP,van Venrooij WJ,Muller S

    更新日期:1997-04-01 00:00:00

  • New insights into non-conventional epitopes as T cell targets: The missing link for breaking immune tolerance in autoimmune disease?

    abstract::The mechanism by which immune tolerance is breached in autoimmune disease is poorly understood. One possibility is that post-translational modification of self-antigens leads to peripheral recognition of neo-epitopes against which central and peripheral tolerance is inadequate. Accumulating evidence points to multiple...

    journal_title:Journal of autoimmunity

    pub_type: 杂志文章,评审

    doi:10.1016/j.jaut.2017.08.001

    authors: Harbige J,Eichmann M,Peakman M

    更新日期:2017-11-01 00:00:00

  • A common epitope on human tumor necrosis factor alpha and the autoantigen 'S-antigen/arrestin' induces TNF-alpha production.

    abstract::A common epitope on S-antigen (arrestin), a potent autoantigen inducing experimental autoimmune uveoretinitis (EAU), and on human tumor necrosis factor alpha (hTNF alpha) was revealed using two monoclonal antibodies to S-antigen which inhibit EAU induction. The minimal common sequence for monoclonal antibody recogniti...

    journal_title:Journal of autoimmunity

    pub_type: 杂志文章

    doi:10.1016/s0896-8411(05)80048-4

    authors: Stiemer RH,Westenfelder U,Gausepohl H,Mirshahi M,Gundt A,Frank RW,Männel DN

    更新日期:1992-02-01 00:00:00

  • Characterization of two anti-DNA antibodies bearing distinct idiotypes. Correlation with clinical manifestations.

    abstract::Spontaneous idiotype shift of anti-DNA antibodies in systemic lupus erythematosus was shown to be associated with changes in the clinical manifestations of the disease. Characterization of two anti-DNA antibody preparations from the same lupus patient showed that lupus nephritis was associated with antibodies showing ...

    journal_title:Journal of autoimmunity

    pub_type: 杂志文章

    doi:10.1016/0896-8411(89)90271-0

    authors: Suenaga R,Munoz PA,Hon SH,Abdou NI

    更新日期:1989-06-01 00:00:00

  • Apoptosis of T cells and macrophages in the central nervous system of intact and adrenalectomized Lewis rats during experimental allergic encephalomyelitis.

    abstract::The adrenocortical response is central to recovery from experimental allergic encephalomyelitis (EAE) in the Lewis rat, as reflected by the increased severity of the disease in adrenalectomized animals. The protection conferred by glucocorticoids is related to the immunosuppressive effects of the steroid, which may in...

    journal_title:Journal of autoimmunity

    pub_type: 杂志文章

    doi:10.1006/jaut.1996.0020

    authors: Smith T,Schmied M,Hewson AK,Lassmann H,Cuzner ML

    更新日期:1996-04-01 00:00:00

  • Autoantibodies from Sjögren's syndrome induce activation of both the intrinsic and extrinsic apoptotic pathways in human salivary gland cell line A-253.

    abstract::Sjögren's syndrome (SS) is an autoimmune rheumatic disease that targets salivary and lachrymal glands, characterized by a high concentration of serum autoantibodies directed against nuclear and cytoplasmic antigens. It is known that autoantibodies can enter viable cells and this phenomenon has functional consequences ...

    journal_title:Journal of autoimmunity

    pub_type: 杂志文章

    doi:10.1016/j.jaut.2006.05.001

    authors: Sisto M,Lisi S,Castellana D,Scagliusi P,D'Amore M,Caprio S,Scagliusi A,Acquafredda A,Panaro MA,Mitolo V

    更新日期:2006-08-01 00:00:00

  • 'Classic' anti-neutrophil cytoplasmic autoantibodies (cANCA), 'Wegener's autoantigen' and their immunopathogenic role in Wegener's granulomatosis.

    abstract::Wegener's autoantigen (WA), a 29 kD multifunctional protein, is the principal target antigen of autoantibodies associated with Wegener's granulomatosis (WG). WA was first identified as proteinase 3 (PR3), which is now known to be identical with myeloblastin and AGP7. Like other lysosomal proteins, WA/PR3 displays enzy...

    journal_title:Journal of autoimmunity

    pub_type: 杂志文章,评审

    doi:10.1006/jaut.1993.1015

    authors: Gross WL,Csernok E,Flesch BK

    更新日期:1993-04-01 00:00:00

  • T-cell maturation and clonal deletion in cyclosporine-induced autoimmunity.

    abstract::In some circumstances cyclosporine (CyA) induces autoimmune phenomena, and a lethal form of syngeneic graft-vs-host disease (SGVHD) can be induced in rats by the administration of CyA. Although several rat strains develop this disease, in this study, we report that of six mouse strains tested, only the DBA/2 strain de...

    journal_title:Journal of autoimmunity

    pub_type: 杂志文章

    doi:10.1016/0896-8411(91)90030-g

    authors: Prud'homme GJ,Sanders R,Parfrey NA,Ste-Croix H

    更新日期:1991-04-01 00:00:00

  • Surface expression of heat shock protein 90 by blood mononuclear cells from patients with systemic lupus erythematosus.

    abstract::Previous studies have indicated that blood mononuclear cells from 15-20% of patients with systemic lupus erythematosus (SLE) carry elevated levels of hsp90, a heat shock protein associated with steroid receptors in cells. We analysed surface expression of hsp90 on mononuclear cells (lymphocytes and monocytes) from pat...

    journal_title:Journal of autoimmunity

    pub_type: 杂志文章

    doi:10.1016/0896-8411(92)90194-u

    authors: Erkeller-Yüksel FM,Isenberg DA,Dhillon VB,Latchman DS,Lydyard PM

    更新日期:1992-12-01 00:00:00

  • Apoptosis and the thymic microenvironment in murine lupus.

    abstract::The thymus of New Zealand black (NZB) mice undergoes premature involution. In addition, cultured thymic epithelial cells from NZB mice undergo accelerated preprogrammed degeneration. NZB mice also have distinctive and well-defined abnormalities of thymic architecture involving stromal cells, defined by staining with m...

    journal_title:Journal of autoimmunity

    pub_type: 杂志文章

    doi:10.1006/jaut.1999.0325

    authors: Takeoka Y,Taguchi N,Shultz L,Boyd RL,Naiki M,Ansari AA,Gershwin ME

    更新日期:1999-11-01 00:00:00

  • The paternally inherited insulin gene B allele (1,428 FokI site) confers protection from insulin-dependent diabetes in families.

    abstract::Several polymorphisms of the insulin gene and its flanking regions (INS region) are in linkage disequilibrium and confer susceptibility to insulin-dependent diabetes (IDDM). We have analysed INS AA and AB-BB genotypes at the 1,428 FokI site (3' of the insulin gene) in 217 patients with IDDM, 402 non-diabetic first deg...

    journal_title:Journal of autoimmunity

    pub_type: 杂志文章

    doi:10.1006/jaut.1994.1053

    authors: Pugliese A,Awdeh ZL,Alper CA,Jackson RA,Eisenbarth GS

    更新日期:1994-10-01 00:00:00

  • Do we know which patients with the antiphospholipid syndrome should receive long-term high dose anti-coagulation?

    abstract::The standard treatment of thrombosis related to antiphospholipid antibodies is lifelong high-intensity oral anticoagulation. This standard is primarily based on retrospective evaluations of such patients. In this paper we give a summary of current treatment of deep leg vein thrombosis, pulmonary embolism, ischaemic st...

    journal_title:Journal of autoimmunity

    pub_type: 杂志文章,评审

    doi:10.1006/jaut.2000.0395

    authors: Derksen RH,de Groot PG

    更新日期:2000-09-01 00:00:00

  • Homing of GAD65 specific autoimmunity and development of insulitis requires expression of both DQ8 and human GAD65 in transgenic mice.

    abstract::MHC-class II genes determine susceptibility in human type-1 diabetes. In their context, presentation of target antigen(s) results in autoimmunity and beta-cell destruction. An animal model, in which human beta-cell autoantigen(s) are presented to effector cells in the context of human MHC-class II diabetes-susceptibil...

    journal_title:Journal of autoimmunity

    pub_type: 杂志文章

    doi:10.1016/j.jaut.2009.02.004

    authors: Elagin RB,Balijepalli S,Diacovo MJ,Baekkeskov S,Jaume JC

    更新日期:2009-08-01 00:00:00

  • Constitutive up-regulated activity of MAP kinase is associated with down-regulated early p21Ras pathway in lymphocytes of SLE patients.

    abstract::Aberrant expression of the p21Ras proto-oncogene has been reported in lymphoid cells of SLE patients. We previously showed that the expression of the p21Ras stimulatory element, hSOS1, is reduced in PBMC from SLE patients with non-active disease. However, the significance of this finding regarding the regulation and f...

    journal_title:Journal of autoimmunity

    pub_type: 杂志文章

    doi:10.1006/jaut.2002.0596

    authors: Rapoport MJ,Amit M,Aharoni D,Weiss M,Weissgarten J,Bruck N,Buchs A,Bistritzer T,Molad Y

    更新日期:2002-08-01 00:00:00

  • Functional characterization of the MECP2/IRAK1 lupus risk haplotype in human T cells and a human MECP2 transgenic mouse.

    abstract::Genetic polymorphism in MECP2/IRAK1 on chromosome Xq28 is a confirmed and replicated susceptibility locus for lupus. High linkage disequilibrium in this locus suggests that both MECP2 and IRAK1 are candidate genes for the disease. DNA methylation changes in lupus T cells play a central role in the pathogenesis of lupu...

    journal_title:Journal of autoimmunity

    pub_type: 杂志文章

    doi:10.1016/j.jaut.2012.12.012

    authors: Koelsch KA,Webb R,Jeffries M,Dozmorov MG,Frank MB,Guthridge JM,James JA,Wren JD,Sawalha AH

    更新日期:2013-03-01 00:00:00

  • Intracellular trafficking of beta2-glycoprotein I complexes with lipid vesicles in macrophages: implications on the development of antiphospholipid syndrome.

    abstract::Beta(2)-glycoprotein I (beta(2)GPI) is known as a major autoantigen for antiphospholipid antibodies. Our recent data show that binding of beta(2)GPI to oxidized low-density lipoprotein (oxLDL) or to liposomes containing anionic phospholipid(s) may facilitate the presentation of beta(2)GPI's epitope by macrophages/dend...

    journal_title:Journal of autoimmunity

    pub_type: 杂志文章

    doi:10.1016/j.jaut.2007.07.003

    authors: Kajiwara T,Yasuda T,Matsuura E

    更新日期:2007-09-01 00:00:00

  • The anti-viral facet of anti-rheumatic drugs: Lessons from COVID-19.

    abstract::The outbreak of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection has posed the world at a pandemic risk. Coronavirus-19 disease (COVID-19) is an infectious disease caused by SARS-CoV-2, which causes pneumonia, requires intensive care unit hospitalization in about 10% of cases and can lead to ...

    journal_title:Journal of autoimmunity

    pub_type: 杂志文章,评审

    doi:10.1016/j.jaut.2020.102468

    authors: Perricone C,Triggianese P,Bartoloni E,Cafaro G,Bonifacio AF,Bursi R,Perricone R,Gerli R

    更新日期:2020-07-01 00:00:00

  • Elimination of infectious antigens and increase of IgG catabolism as possible modes of action of IVIg.

    abstract::Many mechanisms can explain the mode of action of IVIg in immune disorders. Macrophage blockade and interference in the idiotypic network are supported by some experimental data. Among the other mechanisms, two are considered in greater detail. Firstly, in some disorders, the patients could improve simply because the ...

    journal_title:Journal of autoimmunity

    pub_type: 杂志文章,评审

    doi:10.1006/jaut.1993.1057

    authors: Masson PL

    更新日期:1993-12-01 00:00:00

  • Isolation and characterization of apoptotic nucleosomes, free and complexed with lupus autoantibody generated during hybridoma B-cell apoptosis.

    abstract::Increasing evidence suggests that immune complexes made of anti-nuclear antibodies bound to nucleosomes released from dead cells play an important role in the pathogenesis of lupus nephritis. However, the nature and composition of apoptotic nucleosomes still remain elusive. Since large amounts of nucleosomes are relea...

    journal_title:Journal of autoimmunity

    pub_type: 杂志文章

    doi:10.1006/jaut.1997.0172

    authors: Cabrespines A,Laderach D,Lebossé C,Bach JF,Koutouzov S

    更新日期:1998-02-01 00:00:00