Abstract:
:Plague is a re-emerging disease endemic in at least 24 countries. Non-endemic countries should be able to confirm plague to prevent outbreaks due to imported cases. We established a combination of a IgG/IgM screening ELISA and a confirmation immunoblot employing F1 capsular antigen (CA) for the serodiagnosis of plague in countries where yersiniosis is present. The ELISA and the immunoblot assay showed a specificity of 96.1% and 100% among sera from healthy German blood donors. This group had a seroprevalence of 39% of anti-yersinia outer protein (YOP) antibodies obviously caused by previous Y. enterocolitica infection. The ELISA detected anti-F1 CA antibodies in 22 and the immunoblot in 20 out of 26 sera of plague vaccinees. Five control sera from bacteriologically confirmed plague cases from Madagascar reacted positively. It can be concluded that anti-YOP antibodies do not affect assays based on purified F1 CA.
journal_name
Epidemiol Infectjournal_title
Epidemiology and infectionauthors
Neubauer H,Rahalison L,Brooks TJ,Aleksic S,Chanteau S,Splettstösser WDdoi
10.1017/s0950268800004647subject
Has Abstractpub_date
2000-12-01 00:00:00pages
593-7issue
3eissn
0950-2688issn
1469-4409journal_volume
125pub_type
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