Dissociation of DNA binding and in vitro transcriptional activities dependent on the C terminus of P53 proteins.

abstract：:Escherichia coli isoleucyl-tRNA synthetase has been shown to contain two enzyme-bound zinc atoms per polypeptide chain. To investigate the structural and functional significance of the C-terminal enzyme-bound zinc, mutagenesis was used to alter Cys 922 to Ser [IleRS(C922S)] and to replace Cys 922 through Ala 939 with ...

journal_title：Biochemical and biophysical research communications

authors： Zhou L,Rosevear PR

更新日期：1995-11-13 00:00:00

abstract：:Leukotrienes play an important pathophysiological role in chronic inflammatory states and, as previously shown, cause increased levels of cyclooxygenase-2 (COX-2) in intestinal epithelial cells. The anti-apoptotic protein Bcl-2 is also elevated by LTD(4) stimulation, and in colon cancer, so we studied the mechanisms o...

journal_title：Biochemical and biophysical research communications

authors： Wikström K,Ohd JF,Sjölander A

更新日期：2003-03-07 00:00:00

abstract：:We report high resolution images of RecA-double stranded (ds) DNA complexes obtained by atomic force microscopy (AFM). When a carbon nanotube (CNT) tip was used, AFM images visualized the 10-nm pitch of RecA-dsDNA complexes and RecA filaments as three-dimensional surface topography without reconstruction analysis. The...

journal_title：Biochemical and biophysical research communications

authors： Umemura K,Komatsu J,Uchihashi T,Choi N,Ikawa S,Nishinaka T,Shibata T,Nakayama Y,Katsura S,Mizuno A,Tokumoto H,Ishikawa M,Kuroda R

更新日期：2001-02-23 00:00:00

abstract：:Cripto is a cell surface protein highly expressed in certain solid tumors, and overexpression of Cripto protein is oncogenic. Cripto-1 protein is encoded by CRIPTO1 gene. CRIPTO3, a presumed pseudogene, has an open reading frame with six amino acid differences from Cripto-1. We show that CRIPTO3 mRNA is the CRIPTO mes...

journal_title：Biochemical and biophysical research communications

authors： Sun C,Orozco O,Olson DL,Choi E,Garber E,Tizard R,Szak S,Sanicola M,Carulli JP

更新日期：2008-12-05 00:00:00

abstract：:Cigarette smoke is a major risk factor for lung cancer and respiratory infections. This increased susceptibility may result from cigarette smoke-induced impairment of the immune system. In this study, we evaluated the effect of nicotine on the production of inflammatory mediators by activated macrophages. Pretreatment...

journal_title：Biochemical and biophysical research communications

authors： Sugano N,Shimada K,Ito K,Murai S

更新日期：1998-11-09 00:00:00

abstract：:Recent reports have shown that the endoplasmic reticulum (ER) stress is relevant to the pathogenesis of Alzheimer disease. Following the amyloid cascade hypothesis, we therefore attempted to investigate the effects of ER stress on amyloid-beta peptide (Abeta) generation. In this study, we found that ER stress altered ...

journal_title：Biochemical and biophysical research communications

authors： Kudo T,Okumura M,Imaizumi K,Araki W,Morihara T,Tanimukai H,Kamagata E,Tabuchi N,Kimura R,Kanayama D,Fukumori A,Tagami S,Okochi M,Kubo M,Tanii H,Tohyama M,Tabira T,Takeda M

更新日期：2006-06-02 00:00:00

abstract：:A rat liver-specific antigen (RLSA) lost its binding ability to the corresponding monoclonal antibody after treatment with N-glycanase or sialidase, which suggested that the specific binding site might be in a portion of the sugar chain containing sialic acid. The specific antigen reacted with wheat germ agglutinin, l...

journal_title：Biochemical and biophysical research communications

authors： Sato K,Ikeda T,Osada Y

更新日期：1988-05-16 00:00:00

abstract：:Calcitonin gene-related peptide (CGRP) in porcine ventricular muscles. positive inotropic effects in the isolated, electrically driven false tendon of the porcine heart. Specific CGRP-binding sites were present in solubilized membrane fractions; the dissociation constant (Kd) and the maximum binding (Bmax) were 50.4 p...

journal_title：Biochemical and biophysical research communications

authors： Miyauchi T,Sano Y,Hiroshima O,Yuzuriha T,Sugishita Y,Ishikawa T,Saito A,Goto K

更新日期：1988-08-30 00:00:00

abstract：:In contrast to its stimulation of insulin binding to human placental membranes, calcium inhibited the binding of insulin-like growth factor-I. The effects on receptors for both peptides were half-maximal at 2 mM calcium, and were entirely due to alterations in high affinity binding sites for the respective ligands. Ca...

journal_title：Biochemical and biophysical research communications

authors： Baxter RC,Williams PF

更新日期：1983-10-14 00:00:00

abstract：:Anders et al. (2011) [11] reported that cyclinD1/Cdk4 and cyclinD3/Cdk6 enhance the transcriptional activity of FOXM1c by phosphorylating its TAD. They defined 12 Cdk consensus sites as essential for the activation of FOXM1c by cyclinD1/Cdk4 and cyclinD3/Cdk6 and stated that the 12 Cdk-sites are positioned within the ...

journal_title：Biochemical and biophysical research communications

authors： Wierstra I

更新日期：2013-02-22 00:00:00

abstract：:Angiogenesis is a core hallmark of advanced cancers, especially in lung adenocarcinoma (LUAD). However, the underlying functions and mechanisms of lncRNAs in tumor angiogenesis remain largely unknown. Here we found that linc00665 depletion could markedly depressed proliferation and capillary tube formation of HUVECs i...

journal_title：Biochemical and biophysical research communications

authors： Cong Z,Diao Y,Li X,Jiang Z,Xu Y,Zhou H,Qiang Y,Wu H,Shen Y

更新日期：2020-06-25 00:00:00

abstract：:Stomatal movements are regulated by alternate phase transition in the lipophilic domains of membrane initiated by blue light through activation of plasmalemma redox system. ABA blocks the blue light activated redox reactions leading to the accumulation of superoxide (O2.-) radical inducing melting of the membrane and ...

journal_title：Biochemical and biophysical research communications

authors： Purohit S,Kumar GP,Laloraya M,Laloraya MM

更新日期：1994-11-30 00:00:00

abstract：:Porcine kidney betaine aldehyde dehydrogenase (EC 1.2.1.8) kinetic properties were determined at low substrate concentrations. The double-reciprocal plots of initial velocity versus substrate concentration are linear and intersect at the left of the 1/v axis and showed substrate inhibition with betaine aldehyde. Studi...

journal_title：Biochemical and biophysical research communications

authors： Figueroa-Soto CG,Valenzuela-Soto EM

更新日期：2000-03-16 00:00:00

abstract：:We have analyzed the cellular effects of nanosecond pulsed electrical fields on plant cells using fluorescently tagged marker lines in the tobacco cell line BY-2 and confocal laser scanning microscopy. We observe a disintegration of the cytoskeleton in the cell cortex, followed by contraction of actin filaments toward...

journal_title：Biochemical and biophysical research communications

authors： Berghöfer T,Eing C,Flickinger B,Hohenberger P,Wegner LH,Frey W,Nick P

更新日期：2009-09-25 00:00:00

abstract：:Ubiquitylation is an essential posttranslational protein modification that is implicated in a diverse array of cellular functions. Although cells contain eight structurally distinct types of polyubiquitin chains, detailed function of several chain types including K29-linked chains has remained largely unclear. Current...

journal_title：Biochemical and biophysical research communications

authors： Tsuchiya H,Tanaka K,Saeki Y

更新日期：2013-06-28 00:00:00

abstract：:Acute respiratory distress syndrome (ARDS) is associated with high mortality and morbidity. Early diagnosis and risk stratification in patients with ARDS should improve prognosis. Unfortunately, no clinical biomarkers are available for use in early diagnosis. Quantitative proteomics is a powerful tool for biomarker di...

journal_title：Biochemical and biophysical research communications

authors： Ren S,Chen X,Jiang L,Zhu B,Jiang Q,Xi X

更新日期：2016-09-23 00:00:00

abstract：:Laminins, the major basement membrane glycoproteins, are composed of three subunits. We identified a splice variant of the human laminin alpha4 subunit transcript containing 21 extra nucleotides. A heptapeptide sequence, MDCPTIS, was inserted close to the two cysteine residues possibly involved in the intersubunit dis...

journal_title：Biochemical and biophysical research communications

authors： Hayashi Y,Kim KH,Fujiwara H,Shimono C,Yamashita M,Sanzen N,Futaki S,Sekiguchi K

更新日期：2002-12-06 00:00:00

abstract：:The immunoglobulin mu intronic enhancer is a potent B cell-specific transcriptional activator. The enhancer is activated by the appropriate combination of transcription factors, amongst which are ets and bHLH proteins. HMGA1 (formerly HMG-I(Y)) is a demonstrated co-activator of the mu enhancer. HMGA1 functions through...

journal_title：Biochemical and biophysical research communications

authors： Andreucci A,Reeves R,McCarthy KM,Nikolajczyk BS

更新日期：2002-03-29 00:00:00

abstract：:ALIS are large, transient, cytosolic aggregates that serve as storage compartments for ubiquitin-tagged defective ribosomal products. We determined the importance of the protein p62 in the formation of ALIS and demonstrated that two domains of p62-PB1 and UBA-are essential for ALIS assembly. Those two major binding do...

journal_title：Biochemical and biophysical research communications

authors： Cabe M,Rademacher DJ,Karlsson AB,Cherukuri S,Bakowska JC

更新日期：2018-09-18 00:00:00

abstract：:The effect of human recombinant interferons (IFNs) alpha and gamma on the transcription of Herpes Simplex virus type-1 (HSV-1) immediate early (IE) genes has been studied. Human amniotic cells (WISH) were transfected with a chimaeric plasmid containing the regulatory region of the HSV-1 IE genes controlling the expres...

journal_title：Biochemical and biophysical research communications

authors： De Stasio PR,Taylor MW

更新日期：1989-03-15 00:00:00

abstract：:The short-term incubation of HeLa cells in the presence of diferric transferrin or ferricyanide, which are reduced externally by the transplasma membrane reductase, produces a stoichiometric decrease in NADH and increase in NAD+, which is stimulated by insulin. The NADP/NADPH ratio does not change during 15 min incuba...

journal_title：Biochemical and biophysical research communications

authors： Navas P,Sun IL,Morré DJ,Crane FL

更新日期：1986-02-26 00:00:00

abstract：:Vibrio cholerae cytolysin (VCC) is a membrane-damaging beta-barrel pore-forming toxin (beta-PFT). VCC causes permeabilization of the target membranes by forming transmembrane oligomeric beta-barrel pores. Oligomerization is a key step in the mode of action of any beta-PFT, including that of VCC. Earlier studies have i...

journal_title：Biochemical and biophysical research communications

authors： Rai AK,Chattopadhyay K

更新日期：2016-06-03 00:00:00

abstract：:It has long been assumed that Ca2+ are translocated from the cytosol to the cell nucleus by a long distance to activate transcription machinery buried deep in the nucleoplasm. However, this model has been recently challenged. When HeLa cells were loaded with fluo-3, highly fluorescent spots of approximately 2 microns ...

journal_title：Biochemical and biophysical research communications

authors： Lui PP,Kong SK,Kwok TT,Lee CY

更新日期：1998-06-09 00:00:00

abstract：:Endothelial growth factors have become the target of intense research since the initial discovery of vascular endothelial growth factor (VEGF/VPF). At present, VEGF is established as a major inducer of angiogenesis in normal and pathological conditions. Recently several new members in the VEGF family have been describ...

journal_title：Biochemical and biophysical research communications

authors： Farnebo F,Piehl F,Lagercrantz J

更新日期：1999-04-21 00:00:00

abstract：:Rapidly activating Kv4 voltage-gated ion channels are found in heart, brain, and diverse other tissues including colon and uterus. Kv4.3 can co-assemble with KChIP ancillary subunits, which modify kinetic behavior. We examined the affinity and use dependence of nifedipine block on Kv4.3 and its modulation by KChIP2b. ...

journal_title：Biochemical and biophysical research communications

authors： Bett GC,Morales MJ,Strauss HC,Rasmusson RL

更新日期：2006-02-24 00:00:00

abstract：:Molecular analysis of the induction of the lutropin/choriogonadotropin receptor during the process of luteinization of the rat ovary was performed. The appearance of receptor binding activity and an immunological analysis of the receptor using Triton solubilized membrane proteins show little receptor present in luteal...

journal_title：Biochemical and biophysical research communications

authors： Hansbury MJ,McIlroy PJ,Goldenthal MJ

更新日期：1990-09-14 00:00:00

abstract：:Using a synthetic 21-residue endothelin as antigen, we have produced an antiserum for endothelin and developed a specific and sensitive radioimmunoassay (RIA) for endothelin. The minimum detection limit of the RIA was 1 pg/tube. Immunoreactive (ir-) endothelin was extracted from the culture medium by Bondelute C8 colu...

journal_title：Biochemical and biophysical research communications

authors： Naruse M,Naruse K,Kurimoto F,Horiuchi J,Tsuchiya K,Kawana M,Kato Y,Zeng ZP,Sakurai H,Demura H

更新日期：1989-04-28 00:00:00

abstract：:The human immunodeficiency virus type 1 (HIV-1) Tat is a virally encoded protein that dramatically up-regulates viral replication through interactions with the HIV-1 5' long terminal repeat (LTR) and cellular transcription factors. The HIV-1 LTR is divided into three major regions: modulatory, core and TAR. The modula...

journal_title：Biochemical and biophysical research communications

authors： Li X,Josef J,Marasco WA

更新日期：2001-08-24 00:00:00

abstract：:Wheat germ phosphoglycerate mutase, exposed to 3.4 M guanidinium chloride at 22 degrees C and pH 7.8, slowly undergoes time-dependent inactivation which can be fully reversed by adding excess Co2+ or Mn2+ to a 50-fold dilution of the denaturing medium. Titration of the denatured enzyme with either Co2+ or Mn2+ shows t...

journal_title：Biochemical and biophysical research communications

authors： Smith GC,McWilliams AD,Hass LF

更新日期：1986-04-14 00:00:00

abstract：:We investigated the involvement of G(i/o) protein in NO production following the activation of proteinase-activated receptor-4 (PAR-4) in cultured bovine aortic endothelial cells. AYPGKF-NH(2) (PAR-4 activating peptide), thrombin, and ionomycin induced a concentration-dependent NO production, with the maximal producti...

journal_title：Biochemical and biophysical research communications

authors： Momota F,Hirano K,Hirano M,Nishimura J,Kanaide H

更新日期：2006-04-07 00:00:00