Modified salting-out method for DNA isolation from newborn cord blood nucleated cells.

Abstract:

:The present work describes modification of a widely used salting-out procedure to rapidly extract DNA suitable for PCR, using the ARMS method to amplify a target sequence in the beta-globin gene. The salting-out DNA extraction procedure did not completely remove or decrease the presence of inhibitors to PCR in a considerable number of cord blood samples. By introducing a simple phenol/chloroform step, before ethanol precipitation of the nucleic acid, to certain samples, we were able to eliminate or substantially reduce the presence of inhibitors to PCR without having to re-extract the samples.

journal_name

J Clin Lab Anal

authors

Noguera NI,Tallano CE,Bragós IM,Milani AC

subject

Has Abstract

pub_date

2000-01-01 00:00:00

pages

280-3

issue

6

eissn

0887-8013

issn

1098-2825

pii

10.1002/1098-2825(20001212)14:6<280::AID-JCLA6>3.0

journal_volume

14

pub_type

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