Abstract:
PURPOSE:To investigate the induction of chromosomal aberrations in mouse m5S cells after exposure to power-line frequency magnetic fields (extremely low frequency magnetic fields; ELFMF) at high-flux densities. MATERIAL AND METHOD:m5S cells were either untreated or pretreated during the G1 phase with mitomycin C (MMC, 1 microM) for 1 h or 3 Gy X-rays, and then exposed to ELFMF at three different flux densities (5 and 50 mT at 60 Hz, 400 mT at 50 Hz) for 40 h. Unexposed control cells were incubated for the same period in a conventional CO2 incubator. Chromosomal aberrations were analysed in the first post-treatment metaphases. Cell kinetics were assessed by DNA flow cytometry and the mitotic index. RESULTS AND CONCLUSIONS:ELFMF enhanced the formation of spontaneous and MMC- or X-ray-induced chromosomal aberrations, in a flux-density-dependent manner. Statistically significant increases in the frequency of chromosomal aberrations were observed in cells exposed to 400 mT ELFMF with respect to unexposed controls. The aberrations induced by ELFMF were mostly chromatid-type, not chromosome-type. The cells exposed to 400 mT ELFMF exhibited a three-fold higher level of chromatid-type aberrations than did the unexposed cells. Flow cytometric and mitotic index analyses revealed that the S or G2 arrest following MMC or X-irradiation was more profound in ELFMF-exposed cells than in unexposed cells. Our results suggest that ELFMF can interfere with post-replication repair, resulting in increased levels of chromatid-type chromosomal aberrations induced spontaneously and by DNA damaging agents.
journal_name
Int J Radiat Bioljournal_title
International journal of radiation biologyauthors
Yaguchi H,Yoshida M,Ding GR,Shingu K,Miyakoshi Jdoi
10.1080/09553000050201172subject
Has Abstractpub_date
2000-12-01 00:00:00pages
1677-84issue
12eissn
0955-3002issn
1362-3095journal_volume
76pub_type
杂志文章abstract::This study simulates the exposure of V79 Chinese hamster fibroblasts to low-energy protons, deuterons and alpha-particles in the LET range 10-200 keV/microm. The starting assumption is that the induction of clustered lesions in DNA is a fundamental step for cell inactivation. A non-homogeneous cell population was simu...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530097143004
更新日期:1997-11-01 00:00:00
abstract::A radionuclide release assay for measuring the in vitro kinetics of cell death has been developed. CHO cells were labelled for 24 h with 3.0 hBq/ml of [125I] iododeoxyuridine (125IUdR) and the fate of the labelled cells and their progeny was monitored at daily intervals by measuring the rate of 125I release. Prelabell...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009114550361
更新日期:1991-02-01 00:00:00
abstract::The yields of strand break formation (Gssb) in single-stranded DNA (ssDNA) initiated by radiation-generated OH radicals have been determined using the method of low-angle laser light scattering (LALLS). The irradiations were carried out in aqueous, N2O-saturated solutions in the absence and presence of oxygen and at d...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009014551951
更新日期:1990-10-01 00:00:00
abstract:PURPOSE:To determine the relationships between the frequencies of radiation-induced chromosomal alterations and the extent of apoptosis in G0 human lymphocytes. MATERIAL AND METHODS:G0 human peripheral blood lymphocytes (HPBL) were X or gamma-irradiated, in the presence or absence of the repair inhibitor cytosine arab...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553000500303690
更新日期:2005-08-01 00:00:00
abstract:PURPOSE:To evaluate the impact of oral administration of purslane (Portulaca oleracea) extract or fish oil and their co-treatments in the modulation of radiation-induced damage. MATERIAL AND METHODS:Purslane (P) (400 mg/kg body weight) or fish oil (Fo) (60 mg/kg body weight) was administrated to male albino rats via g...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.3109/09553002.2014.926040
更新日期:2014-12-01 00:00:00
abstract:PURPOSE:To investigate and compare the propensity of Epstein-Barr virus (EBV)-transformed lymphoblastoid cell lines (LCL) obtained from unaffected healthy individuals and ataxia telangectasia (A-T) patients to undergo apoptosis after X-ray exposure. MATERIAL AND METHODS:The LCL were exposed to 1-4 Gy X-rays at a dose-...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530099140041
更新日期:1999-06-01 00:00:00
abstract::Human ovarian carcinoma parental and cisplatin-resistant cells were evaluated for their radiation sensitivity to high and low dose-rate irradiation and for the effectiveness of cisplatin in radiosensitization. The cisplatin resistant variant A2780cp showed increased radiation resistance for both low dose-rate (LDRI) a...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530096145634
更新日期:1996-05-01 00:00:00
abstract::Calibration of the non-denaturing (pH 9.6) filter elution technique by 125I decays has been used to examine the relationship between X-ray-induced DNA double-strand breaks (dsb) and cellular survival of CHO cells and human fibroblasts. DNA dsb induction varies markedly between the two cell lines, reflecting the differ...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009314550411
更新日期:1993-03-01 00:00:00
abstract::Skin fibroblasts from ataxia telangiectasia (AT) patients, obligate AT heterozygotes (ATH) and normal individuals were studied for colony-forming ability and repair of DNA double-strand breaks (dsb) after gamma-irradiation. AT cells were three to four times more radiosensitive than normal cells at high and low dose-ra...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009114552601
更新日期:1991-11-01 00:00:00
abstract:PURPOSE:Genotoxic effects have been considered the gold standard to determine if an environmental factor is a carcinogen, but the currently available data for extremely low frequency time-varying magnetic fields (ELF-MF) remain controversial. As an environmental stimulus, the effect of ELF-MF on cellular DNA may be sub...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.3109/09553002.2014.887866
更新日期:2014-04-01 00:00:00
abstract:PURPOSE:The prolonged delivery times associated with intensity modulated radiation therapy (IMRT) may reduce treatment effectiveness of radiation therapy for cancers with short repair half-times. In this study, in vitro radiation experiments with DU-145 prostate cancer cells were designed to quantify the half-time of s...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553000802061285
更新日期:2008-06-01 00:00:00
abstract::The effects of a low dose (0.1-20 mGy) preirradiation with X-rays followed by a higher dose (2 Gy) of the same radiation on the recovery of the genetic damage induced as dominant lethals in mature oocytes (stage 14) of different strains of Drosophila melanogaster were investigated. The response was shown to be depende...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009414550501
更新日期:1994-04-01 00:00:00
abstract:PURPOSE:The effect of boronophenylalanine (BPA)-mediated boron neutron capture therapy (BNCT) on human oral squamous cell carcinoma (SCC) xenografts in nude mice was examined. MATERIALS AND METHODS:Tumor-bearing mice were given BPA at a dose of 250 mg/kg body weight. The tumor (10)B concentration 2 h after an injectio...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553000600570453
更新日期:2006-01-01 00:00:00
abstract:PURPOSE:The efficacy of a boron-containing cholesteryl ester compound (BCH) as a boron neutron capture therapy (BNCT) agent for the targeted irradiation of PC-3 human prostate cancer cells was examined. MATERIALS AND METHODS:Liposome-based delivery of BCH was quantified with inductively coupled plasma-mass spectrometr...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.3109/09553002.2014.901579
更新日期:2014-06-01 00:00:00
abstract:PURPOSE:Cartilage degradation usually results as a consequence of inflammatory processes in the joints. To study this phenomenon experimentally, adjuvant-induced arthritis (AIA) was used as a model of chronic inflammation under the influence of irradiation. The potential chondroprotective effect of 13% aqueous extract ...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.3109/09553002.2011.530337
更新日期:2011-03-01 00:00:00
abstract::Purpose: To summarize existing knowledge and to understand individual response to radiation exposure, the MELODI Association together with CONCERT European Joint Programme has organized a workshop in March 2018 on radiation sensitivity and susceptibility.Methods: The workshop reviewed the current evidence on this matt...
journal_title:International journal of radiation biology
pub_type: 杂志文章,评审
doi:10.1080/09553002.2019.1665209
更新日期:2020-03-01 00:00:00
abstract::A review is presented to the program of education and training setup within the DoReMi Network of Excellence. DoReMi was funded by Euratom under the EU 7th Framework Programme to coordinate the EU research into risks from low-dose ionizing radiation. It was seen to be necessary to form a network of expert institutions...
journal_title:International journal of radiation biology
pub_type: 杂志文章,评审
doi:10.1080/09553002.2018.1454616
更新日期:2019-01-01 00:00:00
abstract::In a series of studies to investigate activation energies and thermal enhancements of various chemotherapeutic agents the effect of 5-fluoruracil (5FU), an antimetabolite, on murine tumour cells was studied at elevated temperatures. Animal tumours were early generation isotransplants of a spontaneous fibrosarcoma, FSa...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009114550211
更新日期:1991-01-01 00:00:00
abstract:PURPOSE:Radioresistance is highly correlated with radiotherapy failure in clinical cancer treatment. In the current study, we sought to examine the efficacy of Celecoxib and Afatinib co-treatment as radiosensitizers in the management of non-small cell lung cancer (NSCLC) A549 cells. MATERIALS AND METHODS:Generally, A5...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553002.2021.1846817
更新日期:2020-11-12 00:00:00
abstract::Wet heat shock (60 degrees C, 90 s) and caffeine (3.8 X 10(-4) M) afford significant radioprotection against post-irradiation O2-dependent damage which develops in seeds of approximately 3.5% moisture content. The damage was assessed in terms of seedling injury on the eighth day of growth. An increase in seedling inju...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009114551101
更新日期:1991-05-01 00:00:00
abstract:PURPOSE:It has been proposed previously that the ratio of complete to incomplete translocations as seen by fluorescence in situ hybridization (FISH), the S value, can be a cytogenetic fingerprint of exposure to radiation of different qualities. Results from a previous study suggested that the S value is approximately 1...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530099140799
更新日期:1999-01-01 00:00:00
abstract:PURPOSE:The hemorrhagic syndrome is a major cause of morbidity and mortality associated with the acute radiation syndrome (ARS). We previously characterized the dose-response relationship for total body irradiation (TBI)-induced ARS in the New Zealand White (NZW) rabbit. Thrombocytopenia, hemorrhage, and anemia were st...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553002.2020.1820601
更新日期:2020-10-16 00:00:00
abstract:PURPOSE:Previous work from the authors' group and others has demonstrated that some of the effects of UV irradiation on gene expression are modulated in response to the addition of salicylic acid to irradiated cells. The presumed effector molecule responsible for this modulation is NF-kappaB. In the experiments describ...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530000138844
更新日期:2000-02-01 00:00:00
abstract:PURPOSE:To investigate the mechanisms inducing and maintaining the permanent elimination of low dose hyper-radiosensitivity (HRS) in cells given a dose of 0.3 Gy at low dose-rate (LDR) (0.3 Gy/h). MATERIALS AND METHODS:Two human HRS-positive cell lines (T-47D, T98G) were used. The effects of pretreatments with transfo...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.3109/09553002.2014.906767
更新日期:2014-07-01 00:00:00
abstract:PURPOSE:To describe the design and dosimetric characterization of a simple and economical small animal irradiator. MATERIALS AND METHODS:A high dose rate (HDR) (192)Ir brachytherapy source from a commercially available afterloader was used with a 1.3 cm thick tungsten collimator to provide sharp beam penumbra suitable...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.3109/09553002.2014.922719
更新日期:2014-10-01 00:00:00
abstract:PURPOSE:Hypoxic cells in tumours are resistant to 5-fluorouracil (5-FU). This in vivo study investigated the ability of hypoxia to regulate the gene expression of thymidylate synthase (TS), the target enzyme of 5-FU. MATERIALS AND METHODS:C3H mammary carcinomas, grown in the feet of female CDF1 mice, were used for all...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530099139944
更新日期:1999-07-01 00:00:00
abstract:PURPOSE:To investigate the influence of chromatin organization and scavenging capacity in relation to irradiation temperature on the induction of double-strand breaks (DSB) in structures derived from human diploid fibroblasts. MATERIALS AND METHODS:Agarose plugs with different chromatin structures (intact cells+/-wort...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553000310001610204
更新日期:2003-10-01 00:00:00
abstract:PURPOSE:Theoretical modelling techniques are often used to simulate the action of ionizing radiations on cells at the nanometre level. Using monoenergetic vacuum-UV (VUV) radiation to irradiate DNA either dry or humidified, the action spectra for the induction of DNA damage by low energy photons and the role of water a...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553000050028913
更新日期:2000-06-01 00:00:00
abstract::We have compared the repair kinetics of X-ray-induced sublethal damage (SLD) and potentially lethal damage (PLD) in V79 cells during postirradiation incubation in growth medium at 37 degrees C. Kinetics of SLD repair were studied by dose fractionation (D1 + D2 = 2 + 2, 4 + 2, 4 + 4, 10 + 4 and 10 + 7 Gy). Kinetics of ...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553008914551411
更新日期:1989-09-01 00:00:00
abstract:PURPOSE:To evaluate and compare cytotoxic and mutational effects of graded doses of gamma-rays and 4He++ ions at different LET values (nominally 80 and 123 keV/microm) in V79 cells. MATERIALS AND METHODS:4He++ ion beams at 80 and 123 keV/microm were supplied by the 7 MV Van de Graaff CN accelerator of the INFN-LNL in ...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553000210146572
更新日期:2002-09-01 00:00:00