Abstract:
:P-glycoprotein (P-gp) and multidrug resistance related protein (MRP) overexpression is often responsible of the development of multidrug resistance in cancer therapy. These proteins are also expressed in normal tissues, where their physiological role is related to the extrusion of endogenous toxins or to secretory function in liver and kidney. The LLC-PK1 cell line is derived from normal pig proximal renal tubule and physiologically expresses low levels of P-gp and MRP. A resistant cell line (LLC-PK1/ADR) has been established in our laboratory by chronic exposure to increasing doses of doxorubicin. Cytofluorimetric analysis of P-gp and MRP expression performed by C219 and MRPm6 immunofluorescence detection showed that these cells overexpress P-gp but not MRP. The uptake of doxorubicin and rhodamine 123 has been quantified in LLC-PK1 and LLC-PK1/ADR cells and compared with data obtained using other tumor cell lines commonly used as reference for studying P-gp or MRP overexpression. P388 sensitive cells and its resistant counterpart P388/ADR cells, which overexpress P-gp and PANC-1 cells, which express high levels of MRP were used. A lower fluorescence intensity was evident with both doxorubicin and rhodamine 123 in LLC-PK1/ADR as well as in P388/ADR cells, that overexpresses P-gp, in comparison with the parental lines. The uptake was increased by a pretreatment with verapamil. Verapamil was completely ineffective on PANC-1 cells, confirming a selective effect of this inhibitor on P-gp. Propidium iodide staining, performed after doxorubicin treatment, confirmed a higher cytotoxicity of the antineoplastic drug in the LLC-PK1 cells compared with the resistant counterpart.
journal_name
Anticancer Resjournal_title
Anticancer researchauthors
Rosati A,Decorti G,Klugmann FB,Candussio L,Granzotto M,Melato M,Giraldi Tsubject
Has Abstractpub_date
2000-09-01 00:00:00pages
3403-10issue
5Beissn
0250-7005issn
1791-7530journal_volume
20pub_type
杂志文章abstract:BACKGROUND/AIM:RASA1 (p120RasGAP), encodes Ras GTPase-activating protein 1 and, is a potent tumor suppressor gene that is frequently inactivated in several human cancer types. However, its precise role in hepatocellular carcinoma (HCC) has been blurred. MATERIALS AND METHODS:We hypothesized that RASA1 plays a crucial ...
journal_title:Anticancer research
pub_type: 杂志文章
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journal_title:Anticancer research
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journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1995-07-01 00:00:00
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journal_title:Anticancer research
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journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2016-07-01 00:00:00
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journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2010-06-01 00:00:00
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journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1999-11-01 00:00:00
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journal_title:Anticancer research
pub_type: 杂志文章
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更新日期:2018-04-01 00:00:00
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journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2003-11-01 00:00:00
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journal_title:Anticancer research
pub_type: 临床试验,杂志文章
doi:
更新日期:1999-03-01 00:00:00
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journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2007-07-01 00:00:00
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journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2013-06-01 00:00:00
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journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1996-09-01 00:00:00
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journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2012-04-01 00:00:00
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journal_title:Anticancer research
pub_type: 杂志文章,评审
doi:
更新日期:1998-05-01 00:00:00
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journal_title:Anticancer research
pub_type: 杂志文章
doi:10.21873/anticanres.13900
更新日期:2019-12-01 00:00:00
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journal_title:Anticancer research
pub_type: 临床试验,杂志文章,随机对照试验
doi:
更新日期:1996-11-01 00:00:00
abstract:BACKGROUND:Recently, 14-member macrolide antibiotics such as Clarithromycin and Roxithromycin (RXM) have been shown to have anti-cancer and anti-angiogeneic effects. However, it is not fully understood whether and how RXM suppresses angiogenesis in human hepatoma, which is a well-known hypervascular tumor. MATERIALS A...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2005-01-01 00:00:00
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journal_title:Anticancer research
pub_type: 杂志文章
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更新日期:2018-05-01 00:00:00
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journal_title:Anticancer research
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doi:
更新日期:2001-03-01 00:00:00
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journal_title:Anticancer research
pub_type: 杂志文章
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journal_title:Anticancer research
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doi:
更新日期:2000-11-01 00:00:00
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journal_title:Anticancer research
pub_type: 临床试验,杂志文章,多中心研究,随机对照试验
doi:
更新日期:2003-11-01 00:00:00
abstract::Basal level of asparagine synthetase mRNA in BALB3T3 cells was elevated when the cells were shifted from medium containing a high concentration (3.3 mM) of asparagine to one lacking asparagine. We then studied whether the expression of asparagine synthetase mRNA is also mediated through other asparagine-independent si...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1995-09-01 00:00:00
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journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1989-05-01 00:00:00
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journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1987-05-01 00:00:00
abstract::Iron overload has been shown to impair the immune response of the liver, and induce hepatic fibrosis and cirrhosis. Opinions differ concerning the relative risk of developing hepatocellular carcinoma (HCC) in siderotic patients as compared with patients with hepatic fibrosis and cirrhosis and the possible mechanism of...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1997-09-01 00:00:00
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journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2005-05-01 00:00:00
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journal_title:Anticancer research
pub_type: 杂志文章
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更新日期:2018-01-01 00:00:00