Abstract：
:The vomeronasal system of mice is thought to be specialized in the detection of pheromones. Two multigene families have been identified that encode proteins with seven putative transmembrane domains and that are expressed selectively in subsets of neurons of the vomeronasal organ. The products of these vomeronasal receptor (Vr) genes are regarded as candidate pheromone receptors. Little is known about their genomic organization and sequence diversity, and only five sequences of mouse V1r coding regions are publicly available. Here, we have begun to characterize systematically the V1r repertoire in the mouse. We isolated 107 bacterial artificial chromosomes (BACs) containing V1r genes from a 129 mouse library. Hybridization experiments indicate that at least 107 V1r-like sequences reside on these BACs. We assembled most of the BACs into six contigs, of which one major contig and one minor contig were characterized in detail. The major contig is 630-860 kb long, encompasses a cluster of 21-48 V1r genes, and contains marker D6Mit227. Sequencing of the coding regions was facilitated by the absence of introns. We determined the sequence of the coding region of 25 possibly functional V1r genes and seven pseudogenes. The functional V1rs can be arranged into three groups; V1rs of one group are novel and substantially divergent from the other V1rs. The genomic and sequence information described here should be useful in defining the biological function of these receptors.
journal_name
Genome Resjournal_title
Genome researchauthors
Del Punta K,Rothman A,Rodriguez I,Mombaerts Pdoi
10.1101/gr.10.12.1958subject
Has Abstractpub_date
2000-12-01 00:00:00pages
1958-67issue
12eissn
1088-9051issn
1549-5469journal_volume
10pub_type
杂志文章相关文献
GENOME RESEARCH文献大全abstract：:Paternal X chromosome inactivation occurs in rodent extraembryonic membranes and in all tissues of marsupials. Methylation of CpG islands occurs on the inactive X in eutherians and is considered to be a stabilizing mechanism. The only previous study of a marsupial X-linked CpG island was of the G6PD gene of the Virgin...
journal_title：Genome research
pub_type： 杂志文章
doi：10.1101/gr.6.2.114
更新日期：1996-02-01 00:00:00
abstract：:The human pseudoautosomal region 1 (PAR1) is essential for meiotic pairing and recombination, and its deletion causes male sterility. Comparative studies of human and mouse pseudoautosomal genes are valuable in charting the evolution of this interesting region, but have been limited by the paucity of genes conserved b...
journal_title：Genome research
pub_type： 杂志文章
doi：10.1101/gr.197001
更新日期：2001-12-01 00:00:00
abstract：:After completion of the Schizosaccharomyces pombe genome sequence, we have carried out a pilot gene deletion project to assess the feasibility of a genome-wide deletion project and to estimate the percentage of essential genes. Using a PCR-based gene deletion procedure, we investigated 100 genes within a 253-kb region...
journal_title：Genome research
pub_type： 杂志文章
doi：10.1101/gr.636103
更新日期：2003-03-01 00:00:00
abstract：:Little is known about the rate of emergence of de novo genes, what their initial properties are, and how they spread in populations. We examined wild yeast populations (Saccharomyces paradoxus) to characterize the diversity and turnover of intergenic ORFs over short evolutionary timescales. We find that hundreds of in...
journal_title：Genome research
pub_type： 杂志文章
doi：10.1101/gr.239822.118
更新日期：2019-06-01 00:00:00
abstract：:We have developed a computer program that aligns spliced sequences to genomic sequences, using local alignment algorithms and heuristics to put together a global spliced alignment. Spidey can produce reliable alignments quickly, even when confronted with noise from alternative splicing, polymorphisms, sequencing error...
journal_title：Genome research
pub_type： 杂志文章
doi：10.1101/gr.195301
更新日期：2001-11-01 00:00:00
abstract：:CTCF is an architectural protein with a critical role in connecting higher-order chromatin folding in pluripotent stem cells. Recent reports have suggested that CTCF binding is more dynamic during development than previously appreciated. Here, we set out to understand the extent to which shifts in genome-wide CTCF occ...
journal_title：Genome research
pub_type： 杂志文章
doi：10.1101/gr.215160.116
更新日期：2017-07-01 00:00:00
abstract：:The reported human genome sequence includes about 400 gaps of unknown sequence that were not found in the bacterial artificial chromosome (BAC) and cosmid libraries used for sequencing of the genome. These missing sequences correspond to approximately 1% of euchromatic regions of the human genome. Gap filling is a lab...
journal_title：Genome research
pub_type： 杂志文章
doi：10.1101/gr.1929904
更新日期：2004-02-01 00:00:00
abstract：:The incorporation and creation of modified nucleobases in DNA have profound effects on genome function. We describe methods for mapping positions and local content of modified DNA nucleobases in genomic DNA. We combined in vitro nucleobase excision with massively parallel DNA sequencing (Excision-seq) to determine the...
journal_title：Genome research
pub_type： 杂志文章
doi：10.1101/gr.174052.114
更新日期：2014-09-01 00:00:00
abstract：:One of the first useful products from the human genome will be a set of predicted genes. Besides its intrinsic scientific interest, the accuracy and completeness of this data set is of considerable importance for human health and medicine. Though progress has been made on computational gene identification in terms of ...
journal_title：Genome research
pub_type： 杂志文章
doi：10.1101/gr.122800
更新日期：2000-10-01 00:00:00
abstract：:We compare the functional spectrum of protein evolution in two separate animal lineages with respect to two hypotheses: (1) rates of divergence are distributed similarly among functional classes within both lineages, indicating that selective pressure on the proteome is largely independent of organismic-level biologic...
journal_title：Genome research
pub_type： 杂志文章
doi：10.1101/gr.2195604
更新日期：2004-05-01 00:00:00
abstract：:Gene order in prokaryotes is conserved to a much lesser extent than protein sequences. Only several operons, primarily those that code for physically interacting proteins, are conserved in all or most of the bacterial and archaeal genomes. Nevertheless, even the limited conservation of operon organization that is obse...
journal_title：Genome research
pub_type： 杂志文章
doi：10.1101/gr.gr-1619r
更新日期：2001-03-01 00:00:00
abstract：:To understand disease mechanisms, a large-scale analysis of human-yeast genetic interactions was performed. Of 1305 human disease genes assayed, 20 genes exhibited strong toxicity in yeast. Human-yeast genetic interactions were identified by en masse transformation of the human disease genes into a pool of 4653 homozy...
journal_title：Genome research
pub_type： 杂志文章
doi：10.1101/gr.211649.116
更新日期：2017-09-01 00:00:00
abstract：:We have developed a novel quantitative method for rapidly assessing the CpG methylation density of a DNA region in mammalian cells. After bisulfite modification of genomic DNA, the region of interest is PCR amplified with primers containing two dam sites (GATC). The purified PCR products are then incubated with 14C-la...
journal_title：Genome research
pub_type： 杂志文章
doi：10.1101/gr.202501
更新日期：2002-01-01 00:00:00
abstract：:We compared the genome of the nematode Caenorhabditis elegans to 13% of that of Caenorhabditis briggsae, identifying 252 conserved segments along their chromosomes. We detected 517 chromosomal rearrangements, with the ratio of translocations to inversions to transpositions being approximately 1:1:2. We estimate that t...
journal_title：Genome research
pub_type： 杂志文章
doi：10.1101/gr.172702
更新日期：2002-06-01 00:00:00
abstract：:Integrating the genotype with epigenetic marks holds the promise of better understanding the biology that underlies the complex interactions of inherited and environmental components that define the developmental origins of a range of disorders. The quality of the in utero environment significantly influences health o...
journal_title：Genome research
pub_type： 杂志文章
doi：10.1101/gr.171439.113
更新日期：2014-07-01 00:00:00
abstract：:Primate pericentromeric regions recently have been shown to exhibit extraordinary evolutionary plasticity. In this paper we report an additional peculiar feature of these regions that we discovered while analyzing, by FISH, the evolutionary conservation of primate phylogenetic chromosome IX. If the position of the cen...
journal_title：Genome research
pub_type： 杂志文章
doi：10.1101/gr.9.12.1184
更新日期：1999-12-01 00:00:00
abstract：:DNA transposons, or class 2 transposable elements, have successfully propagated in a wide variety of genomes. However, it is widely believed that DNA transposon activity has ceased in mammalian genomes for at least the last 40 million years. We recently reported evidence for the relatively recent activity of hAT and H...
journal_title：Genome research
pub_type： 杂志文章
doi：10.1101/gr.071886.107
更新日期：2008-05-01 00:00:00
abstract：:The human Y chromosome, because it is enriched in repetitive DNA, has been very intractable to genetic and molecular analyses. There is no previous evidence for developmental stage- and testis-specific transcription from the male-specific region of the Y (MSY). Here, we present evidence for the first time for a develo...
journal_title：Genome research
pub_type： 杂志文章
doi：10.1101/gr.5155706
更新日期：2007-04-01 00:00:00
abstract：:Transcriptional networks have been shown to evolve very rapidly, prompting questions as to how such changes arise and are tolerated. Recent comparisons of transcriptional networks across species have implicated variations in the cis-acting DNA sequences near genes as the main cause of divergence. What is less clear is...
journal_title：Genome research
pub_type： 杂志文章
doi：10.1101/gr.111765.110
更新日期：2010-12-01 00:00:00
abstract：:The gastrointestinal microbiome undergoes shifts in species and strain abundances, yet dynamics involving closely related microorganisms remain largely unknown because most methods cannot resolve them. We developed new metagenomic methods and utilized them to track species and strain level variations in microbial comm...
journal_title：Genome research
pub_type： 杂志文章
doi：10.1101/gr.142315.112
更新日期：2013-01-01 00:00:00
abstract：:Meiotic DNA double-stranded breaks (DSBs) initiate genetic recombination in discrete areas of the genome called recombination hotspots. DSBs can be directly mapped using chromatin immunoprecipitation followed by sequencing (ChIP-seq). Nevertheless, the genome-wide mapping of recombination hotspots in mammals is still ...
journal_title：Genome research
pub_type： 杂志文章
doi：10.1101/gr.130583.111
更新日期：2012-05-01 00:00:00
abstract：:We have developed a mutation-scanning approach suitable for whole population screening for unknown mutations. The method, meltMADGE, combines thermal ramp electrophoresis with MADGE to achieve suitable cost efficiency and throughput. The sensitivity was tested in blind trials using 54 amplicons representing the BRCA1 ...
journal_title：Genome research
pub_type： 杂志文章
doi：10.1101/gr.3313405
更新日期：2005-07-01 00:00:00
abstract：:Genomics data introduce a substantial computational burden as well as data privacy and ownership issues. Data sets generated by high-throughput sequencing platforms require immense amounts of computational resources to align to reference genomes and to call and annotate genomic variants. This problem is even more pron...
journal_title：Genome research
pub_type： 杂志文章
doi：10.1101/gr.207464.116
更新日期：2018-09-01 00:00:00
abstract：:Establishment of spatial coordinates during Drosophila embryogenesis relies on differential regulatory activity of axis patterning enhancers. Concentration gradients of activator and repressor transcription factors (TFs) provide positional information to each enhancer, which in turn promotes transcription of a target ...
journal_title：Genome research
pub_type： 杂志文章
doi：10.1101/gr.242362.118
更新日期：2019-05-01 00:00:00
abstract：:Comparative analysis of the protein sequences encoded in the four euryarchaeal species whose genomes have been sequenced completely (Methanococcus jannaschii, Methanobacterium thermoautotrophicum, Archaeoglobus fulgidus, and Pyrococcus horikoshii) revealed 1326 orthologous sets, of which 543 are represented in all fou...
journal_title：Genome research
pub_type： 杂志文章
doi：
更新日期：1999-07-01 00:00:00
abstract：:Although more than thirty mammalian genomes have been sequenced to draft quality, very few of these include the Y chromosome. This has limited our understanding of the evolutionary dynamics of gene persistence and loss, our ability to identify conserved regulatory elements, as well our knowledge of the extent to which...
journal_title：Genome research
pub_type： 杂志文章
doi：10.1101/gr.154286.112
更新日期：2013-09-01 00:00:00
abstract：:Chromosomal rearrangements and copy number variants (CNVs) play key roles in genome evolution and genetic disease; however, the molecular mechanisms underlying these types of structural genomic variation are not fully understood. The availability of complete genome sequences for two bird species, the chicken and the z...
journal_title：Genome research
pub_type： 杂志文章
doi：10.1101/gr.103663.109
更新日期：2010-04-01 00:00:00
abstract：:We have generated an improved assembly and gene annotation of the pig X Chromosome, and a first draft assembly of the pig Y Chromosome, by sequencing BAC and fosmid clones from Duroc animals and incorporating information from optical mapping and fiber-FISH. The X Chromosome carries 1033 annotated genes, 690 of which a...
journal_title：Genome research
pub_type： 杂志文章
doi：10.1101/gr.188839.114
更新日期：2016-01-01 00:00:00
abstract：:Next-generation sequencing is a powerful approach for discovering genetic variation. Sensitive variant calling and haplotype inference from population sequencing data remain challenging. We describe methods for high-quality discovery, genotyping, and phasing of SNPs for low-coverage (approximately 5×) sequencing of po...
journal_title：Genome research
pub_type： 杂志文章
doi：10.1101/gr.146084.112
更新日期：2013-05-01 00:00:00
abstract：:The mammalian cell nucleus contains numerous discrete suborganelles named nuclear bodies. While recruitment of specific genomic regions into these large ribonucleoprotein (RNP) complexes critically contributes to higher-order functional chromatin organization, such regions remain ill-defined. We have developed the hig...
journal_title：Genome research
pub_type： 杂志文章
doi：10.1101/gr.237073.118
更新日期：2018-11-01 00:00:00
