Nonlinear determination of Michaelis-Menten kinetics with model evaluation through estimation of uncertainties.

Abstract:

:A structured analytic approach was developed to evaluate enzyme kinetic parameters using non-linear least-squares fitting. The approach was implemented in a software package called KinSim and designed to run on Windows 95, 98, and NT platforms. The software and the theoretical approach were tested using kinetic data obtained using citrate synthase with acetyl CoA as a substrate and octanoyl CoA as an inhibitor. Using the software, the data were evaluated for statistical certainty of the presence of competitive and non-competitive inhibition of the enzyme. Given the determination of the presence of both components of inhibition in the experimental setup, the software was used to determine the inhibitory kinetics from the experimental data, including non-normal distributions of uncertainty. Finally, the software was used to evaluate the sensitivities of the experiment design at each concentration of substrate used. The theoretical approach as implemented in the user-friendly software allows investigators to use a structured procedure for evaluating and planning enzyme kinetic and related metabolic studies.

journal_name

Metab Brain Dis

journal_title

Metabolic brain disease

authors

Mason GF,Lai JC

doi

10.1007/BF02679980

subject

Has Abstract

pub_date

2000-06-01 00:00:00

pages

133-49

issue

2

eissn

0885-7490

issn

1573-7365

journal_volume

15

pub_type

杂志文章
  • Severe infantile epileptic encephalopathy associated with D-glyceric aciduria: report of a novel case and review.

    abstract::D-glycerate 2 kinase (DGK) is an enzyme that mediates the conversion of D-glycerate, an intermediate metabolite of serine and fructose metabolism, to 2-phosphoglycerate. Deficiency of DGK leads to accumulation of D-glycerate in various tissues and its massive excretion in urine. D-glyceric aciduria (DGA) is an autosom...

    journal_title:Metabolic brain disease

    pub_type: 杂志文章,评审

    doi:10.1007/s11011-019-0384-x

    authors: Zehavi Y,Mandel H,Eran A,Ravid S,Abu Rashid M,Jansen EEW,Wamelink MMC,Saada A,Shaag A,Elpeleg O,Spiegel R

    更新日期:2019-04-01 00:00:00

  • Resting-state functional magnetic resonance imaging in hepatic encephalopathy: current status and perspectives.

    abstract::Hepatic encephalopathy (HE) is a neuropsychiatric syndrome which develops in patients with severe liver diseases and/or portal-systemic shunting. Minimal HE, the earliest manifestation of HE, has drawn increasing attention in the last decade. Minimal HE is associated with a series of brain functional changes, such as ...

    journal_title:Metabolic brain disease

    pub_type: 杂志文章,评审

    doi:10.1007/s11011-014-9504-9

    authors: Zhang LJ,Wu S,Ren J,Lu GM

    更新日期:2014-09-01 00:00:00

  • Impaired tissue barriers as potential therapeutic targets for Parkinson's disease and amyotrophic lateral sclerosis.

    abstract::The blood-brain barrier and the intestinal barrier show signs of disruption in patients with idiopathic Parkinson's disease (PD) and animal models of nigrostriatal degeneration, and likewise in amyotrophic lateral sclerosis (ALS) models. A substantial body of evidence shows that defects in epithelial membrane barriers...

    journal_title:Metabolic brain disease

    pub_type: 杂志文章,评审

    doi:10.1007/s11011-018-0239-x

    authors: Fang X

    更新日期:2018-08-01 00:00:00

  • Impact of depressive symptoms and hepatic encephalopathy on health-related quality of life in cirrhotic hepatitis C patients.

    abstract::Depression, common in chronic medical conditions, and hepatic encephalopathy (HE), a reversible neuropsychiatric syndrome due to liver dysfunction, are associated with impaired health-related quality of life (HRQOL) in cirrhosis and hepatitis C (HCV). This study investigated the impact of depression and HE on HRQOL in...

    journal_title:Metabolic brain disease

    pub_type: 杂志文章

    doi:10.1007/s11011-016-9817-y

    authors: Barboza KC,Salinas LM,Sahebjam F,Jesudian AB,Weisberg IL,Sigal SH

    更新日期:2016-08-01 00:00:00

  • Effect of blood ammonia elevation following oral glutamine load on the psychometric performance of cirrhotic patients.

    abstract::Oral glutamine challenge is a method to increase blood ammonia and may be used to study the ammonia lowering effect of drugs potentially useful in hepatic encephalopathy (HE). We tested its influence on the psychometric performance of 18 cirrhotic patients without HE. Twelve nonencephalopatic cirrhotic patients were s...

    journal_title:Metabolic brain disease

    pub_type: 临床试验,杂志文章

    doi:10.1023/a:1021926601907

    authors: Masini A,Efrati C,Merli M,Nicolao F,Amodio P,Del Piccolo F,Riggio O

    更新日期:2003-03-01 00:00:00

  • Post-occlusion treatment with BDNF reduces infarct size in a model of permanent occlusion of the middle cerebral artery in rat.

    abstract::In view of the protective effect of brain derived neurotrophic factor (BDNF) against metabolic/excitotoxic insults in vitro, we investigated whether BDNF could limit infarct size after permanent occlusion of the middle cerebral artery in rat (MCAO). BDNF was delivered into the territory of the middle cerebral artery v...

    journal_title:Metabolic brain disease

    pub_type: 杂志文章

    doi:10.1007/BF02674671

    authors: Yamashita K,Wiessner C,Lindholm D,Thoenen H,Hossmann KA

    更新日期:1997-12-01 00:00:00

  • MicroRNA expression signature of methamphetamine use and addiction in the rat nucleus accumbens.

    abstract::Methamphetamine (METH) is a highly addictive psycho-stimulant that induces behavioral changes due to high level of METH-induced dopamine in the brain. Nucleus accumbens (NAc) plays an important role in these changes, especially in drug addiction. However, little is known about the underlying molecular mechanisms of ME...

    journal_title:Metabolic brain disease

    pub_type: 杂志文章

    doi:10.1007/s11011-017-0061-x

    authors: Sim MS,Soga T,Pandy V,Wu YS,Parhar IS,Mohamed Z

    更新日期:2017-12-01 00:00:00

  • Anxiolytic-like effects of α-asarone in a mouse model of chronic pain.

    abstract::α-asarone (ASR) is a major bioactive compound isolated from the rhizome of Acorus tatarinowii Schott and it has extensive biological effects. Clinically, anxiety disorder is a common comorbidity of chronic pain. However, limited information is available regarding the effects of ASR on chronic pain-related anxiety. Thi...

    journal_title:Metabolic brain disease

    pub_type: 杂志文章

    doi:10.1007/s11011-017-0108-z

    authors: Tian J,Tian Z,Qin SL,Zhao PY,Jiang X,Tian Z

    更新日期:2017-12-01 00:00:00

  • Cortical benzodiazepine receptor binding in a rabbit model of hepatic encephalopathy: the effect of Triton X-100 on receptor solubilization.

    abstract::Increased benzodiazepine (BZ) receptor density has been reported in brains of rabbits with hepatic encephalopathy (HE) due to galactosamine (GalN)-induced fulminant hepatic failure (FHF). These data were generated using detergent-Triton X-100-treated neural membranes. While performing further studies it was noted that...

    journal_title:Metabolic brain disease

    pub_type: 杂志文章

    doi:10.1007/BF01000296

    authors: Rössle M,Mullen KD,Jones EA

    更新日期:1989-09-01 00:00:00

  • Alzheimer's disease and type 2 diabetes: the association study of polymorphisms in tumor necrosis factor-alpha and apolipoprotein E genes.

    abstract::Type 2 diabetes (T2D) and Alzheimer's disease (AD) are two progressive disorders with high prevalence worldwide. Polymorphisms in tumor necrosis factor-alpha (TNF-α) and apolipoprotein E (ApoE) genes might be associated with both T2D and AD, representing possible genetic markers for the development of the AD in subjec...

    journal_title:Metabolic brain disease

    pub_type: 杂志文章

    doi:10.1007/s11011-012-9310-1

    authors: Mustapic M,Popovic Hadzija M,Pavlovic M,Pavkovic P,Presecki P,Mrazovac D,Mimica N,Korolija M,Pivac N,Muck-Seler D

    更新日期:2012-12-01 00:00:00

  • Long term exposure to cell phone frequencies (900 and 1800 MHz) induces apoptosis, mitochondrial oxidative stress and TRPV1 channel activation in the hippocampus and dorsal root ganglion of rats.

    abstract::Mobile phone providers use electromagnetic radiation (EMR) with frequencies ranging from 900 to 1800 MHz. The increasing use of mobile phones has been accompanied by several potentially pathological consequences, such as neurological diseases related to hippocampal (HIPPON) and dorsal root ganglion neuron (DRGN). The ...

    journal_title:Metabolic brain disease

    pub_type: 杂志文章

    doi:10.1007/s11011-017-0180-4

    authors: Ertilav K,Uslusoy F,Ataizi S,Nazıroğlu M

    更新日期:2018-06-01 00:00:00

  • Phosphodiesterase 4B2 gene is an effector of Toll-like receptor signaling in astrocytes.

    abstract::Cyclic AMP is part of an endogenous mechanism that downregulates inflammatory response, and its intracellular concentration is regulated chiefly by cyclic nucleotide phosphodiesterases type 4. The goal of the present study was to determine whether phosphodiesterases 4 are involved in the inflammatory response of astro...

    journal_title:Metabolic brain disease

    pub_type: 杂志文章

    doi:10.1007/s11011-009-9150-9

    authors: Borysiewicz E,Fil D,Dlaboga D,O'Donnell JM,Konat GW

    更新日期:2009-09-01 00:00:00

  • Penetration of glutamate into brain of 7-day-old rats.

    abstract::The permeability of the blood-brain barrier to glutamate was measured by quantitative autoradiography in brains of 7-day-old rats (average plasma glutamate 114 microM) and rats injected subcutaneously with glutamate (average plasma glutamate 2,670 microM). Measurements of glutamate permeability were initiated by the i...

    journal_title:Metabolic brain disease

    pub_type: 杂志文章

    doi:

    authors: Viña JR,DeJoseph MR,Hawkins PA,Hawkins RA

    更新日期:1997-09-01 00:00:00

  • Cordycepin confers neuroprotection in mice models of intracerebral hemorrhage via suppressing NLRP3 inflammasome activation.

    abstract::Neuroinflammation has been recognized as a major contributor to brain injury caused by intracerebral hemorrhage (ICH). Nucleotide-binding oligomerization domain-like receptor family pyrin domain-containing 3 (NLRP3) inflammasome acts as an important mediator of inflammatory response in various inflammation-related dis...

    journal_title:Metabolic brain disease

    pub_type: 杂志文章

    doi:10.1007/s11011-017-0003-7

    authors: Cheng Y,Wei Y,Yang W,Song Y,Shang H,Cai Y,Wu Z,Zhao W

    更新日期:2017-08-01 00:00:00

  • Neuroprotective efficiency of Mangifera indica leaves extract on cadmium-induced cortical damage in rats.

    abstract::Due to the high ability of cadmium to cross the blood-brain barrier, cadmium (Cd) causes severe neurological damages. Hence, the purpose of this study was to investigate the possible protective effect of Mangifera indica leaf extract (MLE) against Cd-induced neurotoxicity. Rats were divided into eight groups. Group 1 ...

    journal_title:Metabolic brain disease

    pub_type: 杂志文章

    doi:10.1007/s11011-018-0222-6

    authors: Al Omairi NE,Radwan OK,Alzahrani YA,Kassab RB

    更新日期:2018-08-01 00:00:00

  • Evidence of hippocampal astrogliosis and antioxidant imbalance after L-tyrosine chronic administration in rats.

    abstract::Tyrosinemia type II is a genetic disorder characterized by elevated blood levels of the amino acid tyrosine caused by the deficiency of tyrosine aminotransferase enzyme, resulting in neurologic and developmental difficulties in the patients. Although neurological sequelae are common in Tyrosinemia type II patients, th...

    journal_title:Metabolic brain disease

    pub_type: 杂志文章

    doi:10.1007/s11011-019-00511-3

    authors: Carvalho-Silva M,Gomes LM,de Prá SD,Wessler LB,Schuck PF,Scaini G,de Bem AF,Blum-Silva CH,Reginatto FH,de Oliveira J,Streck EL

    更新日期:2020-01-01 00:00:00

  • Glutamate receptors in alcohol withdrawal-induced neurotoxicity.

    abstract::Chronic ethanol ingestion results in an "up-regulation" of the N-methyl-D-aspartate (NMDA) subtype of glutamate receptor in mouse brain. This increase in receptors is associated with ethanol withdrawal seizures, which can be attenuated by NMDA receptor antagonists. Chronic exposure to ethanol (3 days) of rat cerebella...

    journal_title:Metabolic brain disease

    pub_type: 杂志文章

    doi:10.1007/BF01991784

    authors: Hoffman PL

    更新日期:1995-03-01 00:00:00

  • Optimization of intracerebroventricular streptozotocin dose for the induction of neuroinflammation and memory impairments in rats.

    abstract::Intracerebroventricular (ICV) injection of streptozotocin (STZ) is a well established procedure to induce neuroinflammation leading to dementia in experimental animals. However, the optimal dose of STZ has not been determined. In the present study, rats were ICV injected with 1.5, 3 and 6 mg of STZ per kg of body weig...

    journal_title:Metabolic brain disease

    pub_type: 杂志文章

    doi:10.1007/s11011-020-00588-1

    authors: Ghosh R,Sil S,Gupta P,Ghosh T

    更新日期:2020-12-01 00:00:00

  • Bipolar limbic expression of auto-immune thyroid targets: thyroglobulin and thyroid-stimulating hormone receptor.

    abstract::The associations between thyroid auto-immunity and neuro-psychiatric disorders are well-documented. However, there exists limited literature specifically linking auto-immune thyroid disease (AITD) to bipolar disorder (BD). Thus, we investigated the likely association between Hashimoto's disease and BD through the extr...

    journal_title:Metabolic brain disease

    pub_type: 杂志文章

    doi:10.1007/s11011-019-00437-w

    authors: Naicker M,Abbai N,Naidoo S

    更新日期:2019-10-01 00:00:00

  • Prolactin, flupenthixol decanoate and first episode schizophrenia - clinical and laboratory correlates.

    abstract::First-episode psychosis (FEP) patients are more sensitive to neuroleptic side-effects such as hyperprolactinemia. We examined the prolactin levels of previously minimally treated patients with first episode schizophrenia over their first year of treatment with flupenthixol decanoate and the relationship between prolac...

    journal_title:Metabolic brain disease

    pub_type: 杂志文章

    doi:10.1007/s11011-019-00474-5

    authors: Retief M,Chiliza B,Phahladira L,Emsley R,Asmal L

    更新日期:2019-12-01 00:00:00

  • Atorvastatin ameliorates cognitive impairment, Aβ1-42 production and Tau hyperphosphorylation in APP/PS1 transgenic mice.

    abstract::Amyloid-beta (Aβ) interacts with the serine/threonine protein kinase AKT (also known as protein kinase B)/glycogen synthase kinase 3β (GSK3β) pathway and deactivates GSK3β signaling, which result in microtubule protein tau phosphorylation. Atorvastatin, a HMG-CoA reductase inhibitor, has been proven to improve learnin...

    journal_title:Metabolic brain disease

    pub_type: 杂志文章

    doi:10.1007/s11011-016-9803-4

    authors: Zhou D,Liu H,Li C,Wang F,Shi Y,Liu L,Zhao X,Liu A,Zhang J,Wang C,Chen Z

    更新日期:2016-06-01 00:00:00

  • Southern Brazilian native fruit shows neurochemical, metabolic and behavioral benefits in an animal model of metabolic syndrome.

    abstract::In this work, we evaluated the effects of Psidium cattleianum (Red Type) (PcRT) fruit extract on metabolic, behavioral, and neurochemical parameters in rats fed with a highly palatable diet (HPD) consisted of sucrose (65% carbohydrates being 34% from condensed milk, 8% from sucrose and 23% from starch, 25% protein and...

    journal_title:Metabolic brain disease

    pub_type: 杂志文章

    doi:10.1007/s11011-018-0262-y

    authors: Oliveira PS,Chaves VC,Soares MSP,Bona NP,Mendonça LT,Carvalho FB,Gutierres JM,Vasconcellos FA,Vizzotto M,Vieira A,Spanevello RM,Reginatto FH,Lencina CL,Stefanello FM

    更新日期:2018-10-01 00:00:00

  • Genetics of alcoholism: role of the endogenous opioid system.

    abstract::At the present time alcoholism is recognized as a metabolic disease exhibiting the clinical features of craving for alcohol, loss of control over drinking, tolerance and physical dependence on alcohol, while both epidemiological and experimental studies have demonstrated that genetic factors may be important in determ...

    journal_title:Metabolic brain disease

    pub_type: 杂志文章,评审

    doi:10.1007/BF01999765

    authors: Gianoulakis C,de Waele JP

    更新日期:1994-06-01 00:00:00

  • Folate supplementation, MTHFR gene polymorphism and neural tube defects: a community based case control study in North India.

    abstract::The present study analyses the potential role of MTHFR gene polymorphism, folate supplementation and dietary pattern among the mothers of NTD neonates and controls in heterogeneous populations of North India, with the special focus on their ethnic labels. Results indicated significant increased risk for neural tube de...

    journal_title:Metabolic brain disease

    pub_type: 杂志文章

    doi:10.1007/s11011-011-9256-8

    authors: Deb R,Arora J,Meitei SY,Gupta S,Verma V,Saraswathy KN,Saran S,Kalla AK

    更新日期:2011-09-01 00:00:00

  • The study of the full spectrum of variants leading to hyperphenylalaninemia have revealed 10 new variants in the PAH gene.

    abstract::This study presents further research into the spectrum of variants in genes responsible for the development of phenylketonuria (PKU) and hyperphenylalaninemia (HPA) in patients in Russia. After a study of 25 frequent variants, 293 patients (327 chromosomes without detected variants) from among 1265 probands still had ...

    journal_title:Metabolic brain disease

    pub_type: 杂志文章

    doi:10.1007/s11011-019-00461-w

    authors: Kuznetcova I,Gundorova P,Ryzhkova O,Polyakov A

    更新日期:2019-12-01 00:00:00

  • Cerebral energy metabolism in hepatic encephalopathy and hyperammonemia.

    abstract::Hepatic encephalopathy (HE) is an important cause of morbidity and mortality in patients with severe liver disease. Although the molecular basis for the neurological disorder in HE remains elusive, elevated ammonia and its chief metabolite glutamine are believed to be important factors responsible for altered cerebral...

    journal_title:Metabolic brain disease

    pub_type: 杂志文章,评审

    doi:10.1023/a:1011666612822

    authors: Rao KV,Norenberg MD

    更新日期:2001-06-01 00:00:00

  • Rhizophora mucronata attenuates beta-amyloid induced cognitive dysfunction, oxidative stress and cholinergic deficit in Alzheimer's disease animal model.

    abstract::Alzheimer's disease (AD) is a progressive neurodegenerative disorder, characterized by accumulation and deposition of Aβ peptide in human brain. The present study aimed to determine the protective effect of catechin rich extract of MERM (methanolic extract of Rhizophora mucronata) on Aβ (25-35) induced cognitive impai...

    journal_title:Metabolic brain disease

    pub_type: 杂志文章

    doi:10.1007/s11011-016-9831-0

    authors: Suganthy N,Malar DS,Devi KP

    更新日期:2016-08-01 00:00:00

  • Increased susceptibility of brain acetylcholinesterase activity to methylmalonate in young rats with renal failure.

    abstract::Tissue methylmalonic acid (MMA) accumulation is the biochemical hallmark of methylmalonic acidemia. Clinically, the disease is characterized by progressive neurological deterioration and renal failure, whose pathophysiology is still undefined. In the present study we investigated the effect of acute MMA administration...

    journal_title:Metabolic brain disease

    pub_type: 杂志文章

    doi:10.1007/s11011-013-9396-0

    authors: Affonso AC,Machado DG,Malgarin F,Fraga DB,Ghedim F,Zugno A,Streck EL,Schuck PF,Ferreira GC

    更新日期:2013-09-01 00:00:00

  • Intracortical inhibitory and excitatory circuits in subjects with minimal hepatic encephalopathy: a TMS study.

    abstract::Minimal hepatic encephalopathy (MHE) is the earliest form of hepatic encephalopathy (HE) and affects up to 80 % of patients with liver cirrhosis. By definition, MHE is characterized by psychomotor slowing and subtle cognitive deficits,  but obvious clinical manifestations are lacking. Given its covert nature, MHE is o...

    journal_title:Metabolic brain disease

    pub_type: 杂志文章

    doi:10.1007/s11011-016-9848-4

    authors: Nardone R,De Blasi P,Höller Y,Brigo F,Golaszewski S,Frey VN,Orioli A,Trinka E

    更新日期:2016-10-01 00:00:00

  • Dietary supplementation of undernourished rats with soy or safflower oil: effects on myelin polyunsaturated fatty acids.

    abstract::Undernourished suckling rats were administered, by gastric intubation, either soy oil (which is rich in both linoleic and linolenic acids) or safflower oil (which is rich in linoleic acid but deficient in linolenic acid) to determine (1) if dietary supplementation would offset the hypomyelination characteristic of the...

    journal_title:Metabolic brain disease

    pub_type: 杂志文章

    doi:10.1007/BF00999385

    authors: Divakaran P,Pavlina T,Johnson RC,Cotter R,Madsen D,Wiggins R

    更新日期:1986-06-01 00:00:00