Abstract:
OBJECTIVE:Ischaemia followed by reperfusion brings about a reduction in cardiac capillary cross-sectional dimensions which is consistent with constriction. The aim of this study was to test the hypothesis that the reduction in cardiac capillary dimensions that occurs in ischaemia and reperfusion is caused by endothelial cell contraction and that modulating the endothelial cell contractile apparatus reduces microvascular reperfusion injury. METHODS:In isolated rat hearts we used phalloidin to stabilise the endothelial actin filaments in order to prevent the dimensional changes during ischaemia. The changes in endothelial cell dimensions were quantified by measuring whole capillary and luminal cross-sectional areas, abluminal and luminal membrane lengths. We have also used resin casts of the coronary vasculature coupled with scanning electron microscopy to examine the structural changes along the length of the capillaries in ischaemia-reperfusion. RESULTS:We found that the reduction in capillary dimensions was prevented by the addition of phalloidin and, in the resin casts, that ischaemia-reperfusion cause focal narrowings along the capillaries which are consistent with constriction. CONCLUSIONS:(1) The endothelial contractile apparatus is involved in the reduction in cross-sectional dimensions. (2) This implies that the capillary bed may have a greater role in the local control of flow than was previously thought and that modulation of the actomyosin contractile system in cardiac capillary endothelial cells may be useful in reducing 'no reflow' injury which results from reperfusion.
journal_name
Cardiovasc Resjournal_title
Cardiovascular researchauthors
Glyn MC,Ward BJdoi
10.1016/s0008-6363(00)00173-5subject
Has Abstractpub_date
2000-11-01 00:00:00pages
346-56issue
2eissn
0008-6363issn
1755-3245pii
S0008-6363(00)00173-5journal_volume
48pub_type
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journal_title:Cardiovascular research
pub_type: 杂志文章,评审
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更新日期:2020-07-31 00:00:00
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pub_type: 杂志文章,评审
doi:10.1016/s0008-6363(01)00420-5
更新日期:2002-01-01 00:00:00
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更新日期:1984-08-01 00:00:00
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更新日期:2013-06-01 00:00:00
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pub_type: 杂志文章
doi:10.1016/0008-6363(95)00216-2
更新日期:1996-02-01 00:00:00
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pub_type: 杂志文章
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更新日期:1986-02-01 00:00:00
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pub_type: 杂志文章
doi:10.1093/cvr/27.11.2005
更新日期:1993-11-01 00:00:00
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journal_title:Cardiovascular research
pub_type: 杂志文章
doi:10.1093/cvr/cvs310
更新日期:2013-02-01 00:00:00
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pub_type: 杂志文章,评审
doi:10.1093/cvr/cvw107
更新日期:2016-07-15 00:00:00
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pub_type: 杂志文章
doi:10.1016/s0008-6363(98)00059-5
更新日期:1998-06-01 00:00:00
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journal_title:Cardiovascular research
pub_type: 杂志文章,评审
doi:10.1016/j.cardiores.2007.06.016
更新日期:2007-09-01 00:00:00
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journal_title:Cardiovascular research
pub_type: 杂志文章
doi:10.1093/cvr/cvp360
更新日期:2010-03-01 00:00:00
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journal_title:Cardiovascular research
pub_type: 杂志文章
doi:10.1016/s0008-6363(98)00270-3
更新日期:1999-04-01 00:00:00
abstract::The structural basis for the development of congestive heart failure (CHF) is a maladaptive myocardial remodeling process which occurs secondarily to post-myocardial infarction (MI), hypertensive hypertrophy, or cardiomyopathy. Both cellular and extracellular factors are involved in the remodeling process and it is th...
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更新日期:2006-02-15 00:00:00
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journal_title:Cardiovascular research
pub_type: 杂志文章
doi:10.1016/j.cardiores.2005.11.020
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doi:10.1093/cvr/cvp160
更新日期:2009-09-01 00:00:00
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journal_title:Cardiovascular research
pub_type: 杂志文章
doi:10.1093/cvr/cvp370
更新日期:2010-03-01 00:00:00
abstract::Estimation of infarct size from enzyme activities in plasma or serum presupposes known values of circulatory parameters such as the extravascular distribution volume Ve and the permeability constant P for the transport of enzyme between intravascular volume Vi and Ve. In man, parameter values are used that are extrapo...
journal_title:Cardiovascular research
pub_type: 杂志文章
doi:10.1093/cvr/13.10.578
更新日期:1979-10-01 00:00:00
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journal_title:Cardiovascular research
pub_type: 杂志文章
doi:
更新日期:1996-09-01 00:00:00
abstract:Aims:The lectin-like oxLDL receptor-1 (LOX-1) promotes endothelial uptake of oxidized low-density lipoprotein (oxLDL) and plays an important role in atherosclerosis and acute coronary syndromes (ACS). However, its role in arterial thrombus formation remains unknown. We investigated whether LOX-1 plays a role in arteria...
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doi:10.1093/cvr/cvx015
更新日期:2017-04-01 00:00:00
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journal_title:Cardiovascular research
pub_type: 杂志文章
doi:10.1016/s0008-6363(98)00011-x
更新日期:1998-05-01 00:00:00
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journal_title:Cardiovascular research
pub_type: 杂志文章
doi:10.1093/cvr/cvn138
更新日期:2008-09-01 00:00:00
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pub_type: 杂志文章
doi:10.1093/cvr/cvu250
更新日期:2015-02-01 00:00:00
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journal_title:Cardiovascular research
pub_type: 杂志文章
doi:10.1016/s0008-6363(03)00330-4
更新日期:2003-06-01 00:00:00