What is the required reperfusion period for assessment of myocardial infarct size using triphenyltetrazolium chloride staining in the rat?

Abstract:

:Measurements of infarct size by use of tripenyltetrazoliumchloride (TTC) is a widely accepted method used to delineate the extent of myocardial necrosis following coronary occlusion and reperfusion in various animal experiments. There is controversy, however, regarding the optimal reperfusion time for estimating the maximally infarcted area by TTC staining in the rat. We tested six different reperfusion times following 90 minutes of regional myocardial ischemia. Group 1 had 5 minutes of reperfusion (n=6), group 2 had 30 minutes of reperfusion (n=6), group 3 had 1 hour of reperfusion (n=6), group 4 had 2 hours of reperfusion (n=6), group 5 had 3 hours of reperfusion (n=6), group 6 had 4.5 hours of reperfusion (n=6). Risk areas, measured by the use of blue dye, were similar among the 6 study groups. Infarct size as a percent of risk area was 57+/-11% in group 1, 74+/-7% in group 2, 61+/-9% in group 3, 71+/-5% in group 4, 70+/-5% in group 5, and 64+/-9% in group 6 (x+/-standard error [SE]). There was no significant difference in infarct size between the groups. However, prior to 60 minutes of reperfusion, patches of pink and white areas were observed within the risk regions, indicating a more difficult assessment of proper delineation of outer regions of necrotic tissue. For acute assessment of infarct size, reperfusion for 60 minutes or more is optimal because the infarct does appear homogeneous at that time and does not become larger with longer reperfusion periods.

journal_name

J Thromb Thrombolysis

authors

Schwarz ER,Somoano Y,Hale SL,Kloner RA

doi

10.1023/a:1018770711705

subject

Has Abstract

pub_date

2000-10-01 00:00:00

pages

181-7

issue

2

eissn

0929-5305

issn

1573-742X

journal_volume

10

pub_type

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