Phenotypic effects of overexpression of the MMAC1 gene in prostate epithelial cells.

Abstract:

:The prostate cancer cell lines PC3 and LNCaP have been shown to lack expression of the tumour suppressor gene MMAC1/PTEN, in contrast to the immortalized non-tumorigenic epithelial lines PNT1a and PNT2. We have measured the effects of reintroduction of wild type (wt) and mutant MMAC1 genes on to these genetic backgrounds, using gene constructs expressing either wt MMAC1 or various mutants deficient in the dual specificity phosphatase domain of the protein. Over-expression of wild type PTEN protein induced cell shrinkage and rounding, but did not result in increased levels of classical apoptosis. Permanently transfected lines containing the MMAC1 gene could only be obtained from the PNT cells, as PTEN expression resulted in rapid loss of both tumour lines. In contrast, mutation of the phosphatase domain resulted in partial attenuation of the phenotypic effects of MMAC1 after transient transfection, and also allowed the derivation of permanent tumour cell lines containing the mutated MMAC1 gene. The results suggest that re-expression of wt PTEN is incompatible with survival of human prostate cancer cells in vitro, and that the full biological activity of this common tumour suppressor requires functions additional to the established protein and lipid phosphatase activities in epithelial systems.

journal_name

Br J Cancer

authors

Sharrard RM,Maitland NJ

doi

10.1054/bjoc.2000.1400

subject

Has Abstract

pub_date

2000-10-01 00:00:00

pages

1102-9

issue

8

eissn

0007-0920

issn

1532-1827

pii

S0007092000914009

journal_volume

83

pub_type

杂志文章
  • Dietary induction of NQO1 increases the antitumour activity of mitomycin C in human colon tumours in vivo.

    abstract::The bioreductive antitumour agent, mitomycin C (MMC), requires activation by reductive enzymes like NAD(P)H:quinone oxidoreductase 1 (NQO1). We used a novel approach to increase MMC efficacy by selectively inducing NQO1 in tumour cells in vivo. CD-1 nude mice were implanted with HCT116 cells, and fed control diet or d...

    journal_title:British journal of cancer

    pub_type: 杂志文章

    doi:10.1038/sj.bjc.6602171

    authors: Begleiter A,Leith MK,Thliveris JA,Digby T

    更新日期:2004-10-18 00:00:00

  • Dihydropyrimidine dehydrogenase deficiency and fluorouracil-related toxicity.

    abstract::Dihydropyrimidine dehydrogenase (DPD) is the initial and rate-limiting enzyme of 5-fluorouracil (5-FU) catabolism. We report lymphocytic DPD data concerning a group of 53 patients (23 men, 30 women, mean age 58, range 36-73), treated by 5-FU-based chemotherapy in different French institutions and who developed unantic...

    journal_title:British journal of cancer

    pub_type: 杂志文章

    doi:10.1038/sj.bjc.6690098

    authors: Milano G,Etienne MC,Pierrefite V,Barberi-Heyob M,Deporte-Fety R,Renée N

    更新日期:1999-02-01 00:00:00

  • High-risk human papillomavirus clearance in pregnant women: trends for lower clearance during pregnancy with a catch-up postpartum.

    abstract::We followed 353 women referred with abnormal cervical cytology in a non-intervention cohort study. In 91 pregnant women we compared high-risk human papilloma virus rates in the subsequent trimesters and postpartum in comparison to 262 non-pregnant women. High-risk human papilloma virus clearance was compared with 179 ...

    journal_title:British journal of cancer

    pub_type: 杂志文章

    doi:10.1038/sj.bjc.6600367

    authors: Nobbenhuis MA,Helmerhorst TJ,van den Brule AJ,Rozendaal L,Bezemer PD,Voorhorst FJ,Meijer CJ

    更新日期:2002-07-01 00:00:00

  • p53 mutations as a marker of malignancy in bladder washing samples from patients with bladder cancer.

    abstract::The diagnosis and follow-up of patients with bladder cancer rely on invasive procedures (cystoscopy with biopsy). Polymerase chain reaction (PCR)-based technologies may allow the sensitive detection of cancer-related genetic mutations in exfoliated tumour material, potentially allowing the development of less invasive...

    journal_title:British journal of cancer

    pub_type: 杂志文章

    doi:10.1054/bjoc.1999.0890

    authors: Phillips HA,Howard GC,Miller WR

    更新日期:2000-01-01 00:00:00

  • Comparison of a new microplate oestrogen receptor (ER) enzyme immunoassay with other ER detection methods.

    abstract::In a study involving 50 breast cancer tumours, we compared two oestrogen receptor (ER) detection methods developed by us--a microplate immunoenzymometric assay (EIA96) and an immunohistochemistry kit (HistoCIS-ER)--with the radioligand assay (RLA), the Abbott immunoenzymometric assay ER-EIA and the reverse transcripta...

    journal_title:British journal of cancer

    pub_type: 杂志文章

    doi:10.1038/bjc.1997.418

    authors: Delage V,Deytieux S,Le Doussal V,Degorce F,Bellanger L,Hacene K,Seguin P,Descotes F,Saez S,Spyratos F

    更新日期:1997-01-01 00:00:00

  • Relationship between p53 status and radiosensitivity in human tumour cell lines.

    abstract::We examined the relationship between p53 levels before and after irradiation, radiation-induced cell cycle delays, apoptotic cell death and radiosensitivity in a panel of eight human tumour cell lines. The cell lines differed widely in their clonogenic survival after radiation, (surviving fraction at 2 Gy: SF2=0.18-0....

    journal_title:British journal of cancer

    pub_type: 杂志文章

    doi:10.1038/bjc.1996.101

    authors: Siles E,Villalobos M,Valenzuela MT,Núñez MI,Gordon A,McMillan TJ,Pedraza V,Ruiz de Almodóvar JM

    更新日期:1996-03-01 00:00:00

  • Variability in functional p53 reactivation by PRIMA-1(Met)/APR-246 in Ewing sarcoma.

    abstract:BACKGROUND:Though p53 mutations are rare in ES, there is a strong indication that p53 mutant tumours form a particularly bad prognostic group. As such, novel treatment strategies are warranted that would specifically target and eradicate tumour cells containing mutant p53 in this subset of ES patients. METHODS:PRIMA-1...

    journal_title:British journal of cancer

    pub_type: 杂志文章

    doi:10.1038/bjc.2013.635

    authors: Aryee DN,Niedan S,Ban J,Schwentner R,Muehlbacher K,Kauer M,Kofler R,Kovar H

    更新日期:2013-11-12 00:00:00

  • Detection of circulating Tg-mRNA in the follow-up of papillary and follicular thyroid cancer: how useful is it?

    abstract::To investigate the usefulness of thyroglobulin mRNA (Tg-mRNA) detection in peripheral blood in the follow-up of papillary and follicular (differentiated) thyroid cancer, a literature study was performed. Both evidence for and evidence against the usefulness of Tg-mRNA detection were found. Also, evidence for the expre...

    journal_title:British journal of cancer

    pub_type: 杂志文章,评审

    doi:10.1038/sj.bjc.6601991

    authors: Verburg FA,Lips CJ,Lentjes EG,de Klerk JM

    更新日期:2004-07-19 00:00:00

  • Comparison of mesna with forced diuresis to prevent cyclophosphamide induced haemorrhagic cystitis in marrow transplantation: a prospective randomised study.

    abstract::A prospective randomised study was carried out to compare the effect of mesna (2-mercaptoethane sulphonate sodium) with that of forced diuresis in preventing cyclophosphamide induced haemorrhagic cystitis in marrow transplant recipients. Sixty-one consecutive BMT recipients were randomised for treatment with forced di...

    journal_title:British journal of cancer

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:10.1038/bjc.1984.252

    authors: Hows JM,Mehta A,Ward L,Woods K,Perez R,Gordon MY,Gordon-Smith EC

    更新日期:1984-12-01 00:00:00

  • N-methylformamide induces changes on adhesive properties and lung-colonizing potential of M14 melanoma cells.

    abstract::We have studied whether N-methylformamide can affect the expression pattern of adhesion molecules and the attachment behaviour of M14 human melanoma cells. The role of N-methylformamide on experimental and spontaneous pulmonary metastases from M14 cells in nude mice was also investigated. We demonstrate that N-methylf...

    journal_title:British journal of cancer

    pub_type: 杂志文章

    doi:10.1038/bjc.1998.35

    authors: Del Bufalo D,Leonetti C,Bucci B,Amedeo C,Falcioni R,Biroccio A,Zupi G

    更新日期:1998-01-01 00:00:00

  • Primary ovarian cancer chemotherapy: current standards of care.

    abstract::Chemotherapy has been regarded as standard therapy for the majority of women with advanced epithelial ovarian cancer for several decades, with this role filled largely by the alkylating agents - used as monotherapy - until the mid-1980s. The activity of cisplatin in this disorder was established during the 1970s, and ...

    journal_title:British journal of cancer

    pub_type: 历史文章,杂志文章,评审

    doi:10.1038/sj.bjc.6601494

    authors: McGuire WP 3rd,Markman M

    更新日期:2003-12-01 00:00:00

  • Influence of misonidazole on the incidence of radiation-induced intestinal tumours in mice.

    abstract::C57BL mice were given local irradiation to 2 cm2 of the lower abdomen in the dose range 16--24 Gy. There were some early deaths, but mice dying between 50--240 days predominantly developed invasive adenocarcinomas of the intestine. When the radiosensitizer misonidazole was given in a single dose shortly before irradia...

    journal_title:British journal of cancer

    pub_type: 杂志文章

    doi:10.1038/bjc.1978.240

    authors: Rostom AY,Kauffman SL,Steel GG

    更新日期:1978-10-01 00:00:00

  • Risk of lung cancer associated with residential radon exposure in south-west England: a case-control study.

    abstract::Studies of underground miners occupationally exposed to radon have consistently demonstrated an increased risk of lung cancer in both smokers and non-smokers. Radon exposure also occurs elsewhere, especially in houses, and estimates based on the findings for miners suggest that residential radon is responsible for abo...

    journal_title:British journal of cancer

    pub_type: 杂志文章

    doi:10.1038/bjc.1998.506

    authors: Darby S,Whitley E,Silcocks P,Thakrar B,Green M,Lomas P,Miles J,Reeves G,Fearn T,Doll R

    更新日期:1998-08-01 00:00:00

  • The impact of young age on cancer-specific and non-cancer-related survival after surgery for colorectal cancer: 10-year follow-up.

    abstract:BACKGROUND:It has been reported that although young patients present with more advanced disease, when adjusted for stage, cancer-specific survival is not different after surgery for colorectal cancer. However, few studies have examined non-cancer survival in young patients and 10-year survival has rarely been reported....

    journal_title:British journal of cancer

    pub_type: 杂志文章

    doi:10.1038/sj.bjc.6605222

    authors: McMillan DC,McArdle CS

    更新日期:2009-08-18 00:00:00

  • Epiregulin gene expression as a biomarker of benefit from cetuximab in the treatment of advanced colorectal cancer.

    abstract:BACKGROUND:Anti-EGFR antibody, cetuximab, improves overall survival (OS) in K-ras wild-type chemotherapy-refractory colorectal cancer. Epidermal growth factor receptor ligand epiregulin (EREG) gene expression may further predict cetuximab benefit. METHODS:Tumour samples from a phase III clinical trial of cetuximab plu...

    journal_title:British journal of cancer

    pub_type: 杂志文章

    doi:10.1038/bjc.2013.753

    authors: Jonker DJ,Karapetis CS,Harbison C,O'Callaghan CJ,Tu D,Simes RJ,Malone DP,Langer C,Tebbutt N,Price TJ,Shapiro J,Siu LL,Wong RP,Bjarnason G,Moore MJ,Zalcberg JR,Khambata-Ford S

    更新日期:2014-02-04 00:00:00

  • Helicobacter pylori and oesophageal and gastric cancers in a prospective study in China.

    abstract::In a cohort of 29,584 residents of Linxian, China, followed from 1985 to 2001, we conducted a case-cohort study of the magnitude of the association of Helicobacter pylori seropositivity with cancer risk in a random sample of 300 oesophageal squamous cell carcinomas, 600 gastric cardia adenocarcinomas, all 363 diagnose...

    journal_title:British journal of cancer

    pub_type: 杂志文章

    doi:10.1038/sj.bjc.6603517

    authors: Kamangar F,Qiao YL,Blaser MJ,Sun XD,Katki H,Fan JH,Perez-Perez GI,Abnet CC,Zhao P,Mark SD,Taylor PR,Dawsey SM

    更新日期:2007-01-15 00:00:00

  • The 1987 Walter Hubert lecture. Regulation and deficiencies in DNA repair.

    abstract::A number of rare human inherited syndromes are associated with apparent defects in DNA repair and a greatly increased frequency of cancer. Cell lines derived from such individuals phenotypically resemble certain bacterial mutant strains that have increased sensitivity to radiation or chemical agents and well character...

    journal_title:British journal of cancer

    pub_type: 杂志文章,评审

    doi:10.1038/bjc.1987.163

    authors: Lindahl T

    更新日期:1987-08-01 00:00:00

  • Non-steroidal anti-inflammatory drugs and risk of gastric and oesophageal adenocarcinomas: results from a cohort study and a meta-analysis.

    abstract::Use of aspirin or other non-steroidal anti-inflammatory drugs (NSAIDs) may reduce the risk of gastric or oesophageal adenocarcinomas. We examined the association between self-reported use of aspirin or non-aspirin NSAIDs in the earlier 12 months and gastric non-cardia (N=182), gastric cardia (N=178), and oesophageal a...

    journal_title:British journal of cancer

    pub_type: 杂志文章,meta分析

    doi:10.1038/sj.bjc.6604880

    authors: Abnet CC,Freedman ND,Kamangar F,Leitzmann MF,Hollenbeck AR,Schatzkin A

    更新日期:2009-02-10 00:00:00

  • Loss of acetoacetate coenzyme A transferase activity in tumours of peripheral tissues.

    abstract::The presence of succinyl-coenzyme A: acetoacetate CoA--transferase (3-oxo acid-CoA transferase), an initiator of ketone body utilization in non-hepatic tissue was examined in a number of animal and human tumours of peripheral tissues. While enzyme levels in heart, kidney, lymphocytes and bladder were high, the tumours...

    journal_title:British journal of cancer

    pub_type: 杂志文章

    doi:10.1038/bjc.1983.38

    authors: Tisdale MJ,Brennan RA

    更新日期:1983-02-01 00:00:00

  • Clinical-guide risk prediction of hepatocellular carcinoma development in chronic hepatitis C patients after interferon-based therapy.

    abstract:BACKGROUND:Interferon (IFN)-based therapies could eradicate hepatitis C (HCV) and reduce the risk of hepatocellular carcinoma (HCC). However, HCC could still happen after sustained virological response (SVR). We aimed to develop a simple scoring system to predict the risk of HCC development among HCV patients after ant...

    journal_title:British journal of cancer

    pub_type: 杂志文章

    doi:10.1038/bjc.2013.564

    authors: Chang KC,Wu YY,Hung CH,Lu SN,Lee CM,Chiu KW,Tsai MC,Tseng PL,Huang CM,Cho CL,Chen HH,Hu TH

    更新日期:2013-10-29 00:00:00

  • A phase I/II study of combined weekly systemic cisplatin and locoregional hyperthermia in patients with previously irradiated recurrent carcinoma of the uterine cervix.

    abstract::We investigated the feasibility and the anti-tumour activity of weekly cisplatin and the simultaneous application of local hyperthermia in patients with a pelvic recurrence of cervical cancer in previously irradiated area. Dose levels of cisplatin 60 mg m(-2), 70 mg m(-2) and 80 mg m(-2) were studied. Treatment object...

    journal_title:British journal of cancer

    pub_type: 临床试验,杂志文章

    doi:10.1038/sj.bjc.6690533

    authors: de Wit R,van der Zee J,van der Burg ME,Kruit WH,Logmans A,van Rhoon GC,Verweij J

    更新日期:1999-07-01 00:00:00

  • Upregulation of p27 and its inhibition of CDK2/cyclin E activity following DNA damage by a novel platinum agent are dependent on the expression of p21.

    abstract::The cisplatin analogue 1R,2R-diaminocyclohexane(trans-diacetato)(dichloro)platinum(IV) (DAP) is a DNA-damaging agent that will be entering clinical trials for its potent cytotoxic effects against cisplatin-resistant tumour cells. This cytotoxicity may reside in its ability to selectively activate G1-phase checkpoint r...

    journal_title:British journal of cancer

    pub_type: 杂志文章

    doi:10.1038/sj.bjc.6603448

    authors: He G,Kuang J,Huang Z,Koomen J,Kobayashi R,Khokhar AR,Siddik ZH

    更新日期:2006-12-04 00:00:00

  • Hybrid Capture II-based human papillomavirus detection, a sensitive test to detect in routine high-grade cervical lesions: a preliminary study on 1518 women.

    abstract::Hybrid Capture II (HC-II) is a commercial human papillomavirus (HPV) detection test designed to detect 18 HPV types divided into high-risk and low-risk groups. We have tested 1647 scrapes from 1518 unselected women attending routine cytological screening by this assay for the detection of histologically proven high-gr...

    journal_title:British journal of cancer

    pub_type: 杂志文章

    doi:10.1038/sj.bjc.6690523

    authors: Clavel C,Masure M,Bory JP,Putaud I,Mangeonjean C,Lorenzato M,Gabriel R,Quereux C,Birembaut P

    更新日期:1999-07-01 00:00:00

  • Combination goserelin and tamoxifen therapy in premenopausal advanced breast cancer: a multicentre study by the ITMO group. Italian Trials in Medical Oncology.

    abstract::It has been suggested that tamoxifen may improve the efficacy of medical castration with luteinising hormone-releasing hormone analogues, but very few data have so far been published concerning the clinical and endocrinological activity of this therapeutic modality. In this phase II multicentre trial conducted by the ...

    journal_title:British journal of cancer

    pub_type: 临床试验,杂志文章,多中心研究

    doi:10.1038/bjc.1995.215

    authors: Buzzoni R,Biganzoli L,Bajetta E,Celio L,Fornasiero A,Mariani L,Zilembo N,Di Bartolomeo M,Di Leo A,Arcangeli G

    更新日期:1995-05-01 00:00:00

  • Changes in serotonin metabolism in cancer patients: its relationship to nausea and vomiting induced by chemotherapeutic drugs.

    abstract::The metabolism of serotonin was studied in cancer patients of their first day of their first course of chemotherapeutic drugs either with strongly or moderately emetogenic regimens. It was observed that strongly emetogenic treatments induce greater increases in serotonin release than moderately emetogenic regimens. Hi...

    journal_title:British journal of cancer

    pub_type: 杂志文章

    doi:10.1038/bjc.1992.242

    authors: Cubeddu LX,Hoffmann IS,Fuenmayor NT,Malave JJ

    更新日期:1992-07-01 00:00:00

  • A multicenter phase 1 study of PX-866 in combination with docetaxel in patients with advanced solid tumours.

    abstract:BACKGROUND:This phase I, dose-finding study determined the safety, maximum tolerated dose (MTD)/recommended phase 2 dose (RP2D), pharmacokinetics, and antitumour activity of PX-866, a phosphatidylinositol 3-kinase inhibitor, combined with docetaxel in patients with incurable solid tumours. METHODS:PX-866 was administe...

    journal_title:British journal of cancer

    pub_type: 杂志文章,多中心研究

    doi:10.1038/bjc.2013.474

    authors: Bowles DW,Ma WW,Senzer N,Brahmer JR,Adjei AA,Davies M,Lazar AJ,Vo A,Peterson S,Walker L,Hausman D,Rudin CM,Jimeno A

    更新日期:2013-09-03 00:00:00

  • Analysis of acute vascular damage after photodynamic therapy using benzoporphyrin derivative (BPD).

    abstract::Benzoporphyrin derivative monoacid ring A (BPD-MA, verteporfin) is currently under investigation as a photosensitizer for photodynamic therapy (PDT). Since BPD exhibits rapid pharmacokinetics in plasma and tissues, we assessed damage to tumour and muscle microvasculature when light treatment for PDT was given at short...

    journal_title:British journal of cancer

    pub_type: 杂志文章

    doi:10.1038/sj.bjc.6690271

    authors: Fingar VH,Kik PK,Haydon PS,Cerrito PB,Tseng M,Abang E,Wieman TJ

    更新日期:1999-04-01 00:00:00

  • Clinical relevance of KRAS mutation detection in metastatic colorectal cancer treated by Cetuximab plus chemotherapy.

    abstract::The predictive value of KRAS mutation in metastatic colorectal cancer (MCRC) patients treated with cetuximab plus chemotherapy has recently been suggested. In our study, 59 patients with a chemotherapy-refractory MCRC treated with cetuximab plus chemotherapy were included and clinical response was evaluated according ...

    journal_title:British journal of cancer

    pub_type: 杂志文章

    doi:10.1038/sj.bjc.6603685

    authors: Di Fiore F,Blanchard F,Charbonnier F,Le Pessot F,Lamy A,Galais MP,Bastit L,Killian A,Sesboüé R,Tuech JJ,Queuniet AM,Paillot B,Sabourin JC,Michot F,Michel P,Frebourg T

    更新日期:2007-04-23 00:00:00

  • A case-control study of head and neck cancer in the Republic of Ireland.

    abstract::A retrospective case-control study of 200 patients with head and neck cancer, and 200 controls matched for age and sex, confirmed the importance of tobacco and alcohol consumption in the aetiology of malignant tumours of the upper gastrointestinal and upper respiratory tracts. A male-female ratio of 3:1 was found, and...

    journal_title:British journal of cancer

    pub_type: 杂志文章

    doi:10.1038/bjc.1981.26

    authors: Herity B,Moriarty M,Bourke GJ,Daly L

    更新日期:1981-02-01 00:00:00

  • Familial aggregation of lung cancer in a high incidence area in China.

    abstract::To investigate whether lung cancer clusters in families in a high incidence county of China, an analysis was conducted using data on domestic fuel history and tobacco use for family members of 740 deceased lung cancer probands and 740 controls (probands' spouses). Lung cancer prevalence was compared among first-degree...

    journal_title:British journal of cancer

    pub_type: 杂志文章

    doi:10.1038/sj.bjc.6602465

    authors: Jin YT,Xu YC,Yang RD,Huang CF,Xu CW,He XZ

    更新日期:2005-04-11 00:00:00