Abstract:
:The possibility of an interaction between the cytoskeletal protein dystrophin and cell surface caveolae in the mammalian myocardium was investigated by several techniques. Caveolin (cav)-3-enriched, detergent-insoluble membranes isolated from purified ventricular sarcolemma by density-gradient fractionation were found to contain dystrophin and dystroglycan. Further purification of cav-3-containing membranes by immunoprecipitation using anti-cav-3-coated magnetic beads yielded dystrophin but not always dystroglycan. Electron microscopic analysis of precipitated material revealed caveola-sized vesicular profiles that could be double-labeled with anti-dystrophin and anti-cav-3 antibodies. In contrast, immunoprecipitation of membranes with anti-dystrophin-coated beads yielded both cav-3 and dystroglycan. Electron microscopic analysis of this material showed heterogeneous membrane profiles, some of which could be decorated with anti-cav-3 antibodies. To confirm that dystrophin and cav-3 were closely associated in cardiac myocytes, we verified that dystrophin was also present in immunoprecipitated cav-3-containing membranes from detergent extracts, as well as in sonicated extracts of purified ventricular myocytes. Confocal immunofluorescence microscopy of ventricular and atrial cardiac myocytes showed that the cellular distributions of cav-3 and dystrophin partially overlapped. Immuno-electron micrographs of thin sections of rat atrial myocytes revealed a fraction of dystrophin molecules that are in apparently close apposition to caveolae. These results suggest that a subpopulation of dystrophin molecules interacts with cardiac myocyte caveolae in vivo and that some of the dystrophin is engaged in linking cav-3 with the dystroglycan complex.
journal_name
Circ Resjournal_title
Circulation researchauthors
Doyle DD,Goings G,Upshaw-Earley J,Ambler SK,Mondul A,Palfrey HC,Page Edoi
10.1161/01.res.87.6.480subject
Has Abstractpub_date
2000-09-15 00:00:00pages
480-8issue
6eissn
0009-7330issn
1524-4571journal_volume
87pub_type
杂志文章abstract::Propagation in cardiac muscle is faster in the longitudinal than in the transverse axis of the cells. Yet, as a result of the larger upstroke velocity of action potentials propagating transversely, it has been suggested that longitudinal propagation is more vulnerable to block. To study the relation between conduction...
journal_title:Circulation research
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doi:10.1161/01.res.60.5.780
更新日期:1987-05-01 00:00:00
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pub_type: 杂志文章,评审
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更新日期:2006-03-03 00:00:00
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doi:10.1161/01.res.82.1.13
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pub_type: 杂志文章,评审
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更新日期:2006-10-27 00:00:00
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pub_type: 杂志文章
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更新日期:1994-12-01 00:00:00
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pub_type: 杂志文章
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更新日期:2005-02-18 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章,评审
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更新日期:2014-05-09 00:00:00
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pub_type: 杂志文章
doi:10.1161/01.res.78.1.82
更新日期:1996-01-01 00:00:00
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pub_type: 杂志文章
doi:10.1161/01.res.85.5.403
更新日期:1999-09-03 00:00:00
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pub_type: 杂志文章
doi:10.1161/CIRCRESAHA.111.254573
更新日期:2011-10-28 00:00:00
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pub_type: 杂志文章
doi:10.1161/01.res.57.6.864
更新日期:1985-12-01 00:00:00
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更新日期:2000-10-27 00:00:00
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pub_type: 临床试验,杂志文章
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更新日期:1993-12-01 00:00:00
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更新日期:2010-05-28 00:00:00
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更新日期:1979-02-01 00:00:00
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pub_type: 杂志文章
doi:10.1161/01.res.64.6.1190
更新日期:1989-06-01 00:00:00
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pub_type: 杂志文章
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更新日期:2007-10-26 00:00:00
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pub_type: 新闻
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更新日期:2010-10-29 00:00:00
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pub_type: 杂志文章
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更新日期:1986-01-01 00:00:00