Endothelial dysfunction after repeated Chlamydia pneumoniae infection in apolipoprotein E-knockout mice.


BACKGROUND:Arterial relaxation is largely regulated by endothelial nitric oxide (NO). Its diminished activity has been associated with incipient atherosclerosis. We investigated the endothelium-dependent relaxation of aorta in apolipoprotein E-knockout (apoE-KO) mice exposed to single or repeated Chlamydia pneumoniae inoculation. METHODS AND RESULTS:Forty-eight apoE-KO mice, 8 weeks old, were inoculated intranasally with C pneumoniae (n=24) or saline (n=24) every 2 weeks over a 6-week period. Twenty mice (10 infected and 10 controls) were killed at 2 weeks and 6 weeks, respectively, after the first inoculation. The smooth muscle tone of aortic rings was measured in vitro at both time points. The norepinephrine-precontracted thoracic aortic rings were successively exposed to methacholine in the absence and presence of N:(G)-nitro-L-arginine methyl ester (L-NAME) and diclofenac. The methacholine-induced relaxation was attenuated in the infected mice at 6 weeks in both the absence and presence of L-NAME (P:<0.05 and P:<0.01, respectively). When administered together with L-NAME, diclofenac enhanced the relaxation of the L-NAME-pretreated aortas in infected mice at 2 weeks (P:<0.05) but not in noninfected mice. The relaxation response from infected mice tended to differ in the same manner at 6 weeks (P:<0.1). No intimal thickening was detected at either time point. CONCLUSIONS:C pneumoniae impairs arterial endothelial function, and the NO pathway is principally involved. Cyclooxygenase-dependent vasoconstricting products may also account for the infection-induced impaired relaxation. These findings further support the role of C pneumoniae infection in atherosclerosis development.






Liuba P,Karnani P,Pesonen E,Paakkari I,Forslid A,Johansson L,Persson K,Wadström T,Laurini R




Has Abstract


2000-08-29 00:00:00












  • Genetic components of the circadian clock regulate thrombogenesis in vivo.

    abstract:BACKGROUND:Myocardial infarction, stroke, and sudden death undergo diurnal variation. Although genes relevant to hemostasis and vascular integrity undergo circadian oscillation, the role of the molecular clock in thrombotic events remains to be established. METHODS AND RESULTS:A diurnal variation in the time to thromb...


    pub_type: 杂志文章


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  • Changes in intracardiac blood flow velocities and right and left ventricular stroke volumes with gestational age in the normal human fetus: a prospective Doppler echocardiographic study.

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    pub_type: 杂志文章


    authors: Kenny JF,Plappert T,Doubilet P,Saltzman DH,Cartier M,Zollars L,Leatherman GF,St John Sutton MG

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    更新日期:1986-01-01 00:00:00

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    pub_type: 杂志文章,多中心研究,随机对照试验


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    pub_type: 杂志文章


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    pub_type: 历史文章,杂志文章


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