Abstract:
INTRODUCTION:Previous studies have suggested a direct relationship between the nasopharyngeal carriage of potential middle ear pathogens and the development of middle ear disease. It has been shown that otitis-prone (OP) children tend to be colonized more often than non-OP children. To study the turnover of nontypeable Haemophilus influenzae (NTHI) in the nasopharynx of OP children, arbitrarily primed PCR was applied to NTHI strains isolated nasopharyngeal swabs collected prospectively during a 2-year study period from 35 OP children under 4 years of age at fixed intervals. METHODOLOGY/MATERIALS: In 20 patients, H. influenzae could be isolated from different sites (left and/or right ear and/or nasopharynx) or at different occasions during follow-up. Forty-eight H. influenzae isolates of different sites (left and/or right ear and/or nasopharynx) of the same patient as well as from siblings were typed using arbitrarily primed PCR with primer ERIC2 and RAPD Ready-to-Go beads (Pharmacia Biotech, Uppsala, Sweden). RESULTS:Typing with arbitrarily primed PCR enabled to differentiate 29 genotypes among the 48 H. influenzae isolates. Sixteen of these fingerprints were observed only once. Thirteen of these fingerprints appeared on two or more occasions. In the three pairs of siblings the same strain was identified at one moment. Genetically identical NTHI strains from unrelated individuals were never identified. In 11 of 14 cases for which isolates were obtained simultaneously from different sites (throat and/or left ear and/or right ear) or from three pairs of siblings, identical fingerprints were observed. In nine cases whereby isolation was separated by a period of more than 4 weeks (maximum 28 weeks) the fingerprints of the isolates were different from the original isolate. CONCLUSION:Typing with arbitrarily primed PCR indicated substantial genetic diversity among the H. influenzae isolates studied, since for a total of 48 isolates of 20 different patients, 29 different genotypes were observed. Since simultaneous isolation for different sampling sites (ear or nasopharynx) as well as for both of siblings, resulted mostly in identical fingerprints, and since sampling of the same site of the same patient, separated by an interval of more than 1 month, almost always resulted in different genotypes, one could conclude that both cross colonization (between sampling sites within the same patient and between siblings) and turnover are high. The relation between acquisition and development of disease needs further attention.
journal_name
Int J Pediatr Otorhinolaryngoljournal_title
International journal of pediatric otorhinolaryngologyauthors
Dhooge I,Vaneechoutte M,Claeys G,Verschraegen G,Van Cauwenberge Pdoi
10.1016/s0165-5876(00)00321-9subject
Has Abstractpub_date
2000-08-11 00:00:00pages
7-12issue
1eissn
0165-5876issn
1872-8464pii
S0165-5876(00)00321-9journal_volume
54pub_type
杂志文章abstract:OBJECTIVES:The molecular etiology of nonsyndromic deafness in Chinese population has not been investigated systematically, our study is aim to investigate the molecular etiology of nonsyndromic deafness patients from Northern China (Heilongjiang province), in order to provide genetic test and counseling to families. M...
journal_title:International journal of pediatric otorhinolaryngology
pub_type: 杂志文章
doi:10.1016/j.ijporl.2017.04.018
更新日期:2017-07-01 00:00:00
abstract::One hundred seventy children and 103 adults from a rural Nigerian village were examined for the presence of middle and external ear pathology. Ear, nose and throat examination included: (1) pneumatic otoscopic exam, (2) tympanometry and (3) contralateral acoustic reflex. A diagnostic algorithm was applied to these 3 f...
journal_title:International journal of pediatric otorhinolaryngology
pub_type: 杂志文章
doi:10.1016/s0165-5876(83)80004-4
更新日期:1983-01-01 00:00:00
abstract:IMPORTANCE:Peristomal pathologies in tracheostomized children are common and often difficult to treat. They may preclude decannulation even after the initial pathology that required tracheostomy had been resolved. OBJECTIVE:We evaluated the safety and effectiveness of combined direct laryngoscopy and trans-stomal endo...
journal_title:International journal of pediatric otorhinolaryngology
pub_type: 杂志文章
doi:10.1016/j.ijporl.2020.110210
更新日期:2020-10-01 00:00:00
abstract::From 1940 to September 2008, 21 cases with TDCs extending into respiratory tract have been reported, often with misdiagnosis. There were only 10 cases by the end of 2006, but the number increased rapidly recently. To evaluate endoscopic carbon dioxide (CO(2)) laser procedure for thyroglossal duct cyst (TDC) extending ...
journal_title:International journal of pediatric otorhinolaryngology
pub_type: 杂志文章,评审
doi:10.1016/j.ijporl.2009.03.016
更新日期:2009-07-01 00:00:00
abstract:OBJECTIVES:To evaluate outcome of surgical excision of sialoblastoma in the parotid gland in newborn. PATIENTS AND METHODS:This was a retrospective review of 3 pediatric patients with sialoblastoma in the parotid gland that underwent surgical resections. All patients are newborn boys. The lesions ranged from 5 cm × 5 ...
journal_title:International journal of pediatric otorhinolaryngology
pub_type: 杂志文章
doi:10.1016/j.ijporl.2013.05.006
更新日期:2013-08-01 00:00:00
abstract:OBJECTIVES:To demonstrate the technique and efficacy of powered assisted adenoidectomy using nasal endoscopy. PATIENTS AND METHODS:Between 2002 and 2003, forty children (age ranged from 3 to 17 years) with symptoms and signs suggestive of snoring and/or obstructive sleep apnea and choanal adenoids were randomly select...
journal_title:International journal of pediatric otorhinolaryngology
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.ijporl.2009.08.019
更新日期:2009-12-01 00:00:00
abstract::Cervical teratomas are rare but life-threatening neonatal tumors and management of the fetus with a cervical teratoma that threatens the airway remains a clinical challenge. This has been revolutionized by advances in fetal imaging and management of the airway at delivery including the use of Ex-utero Intrapartum Trea...
journal_title:International journal of pediatric otorhinolaryngology
pub_type: 杂志文章
doi:10.1016/j.ijporl.2012.03.010
更新日期:2012-07-01 00:00:00
abstract:OBJECTIVES:To identify etiologies of congenital nasal obstruction and describe clinical practice patterns in the evaluation, diagnosis, and treatment of symptomatic infants. METHODS:An electronic chart review from 1/1/2006-10/1/2016 for all patients with a diagnosis of nasal obstruction within the first six months of ...
journal_title:International journal of pediatric otorhinolaryngology
pub_type: 杂志文章,评审
doi:10.1016/j.ijporl.2017.05.023
更新日期:2017-08-01 00:00:00
abstract::The objective of the current study was to investigate possible differences in word count use per day (number of adult words) by caregivers of different gender, in a sample of Norwegian children (N = 17) with hearing impairment (HI) (n = 8) and normal hearing (NH) (n = 9), aged 18-56 months. The current study had a cro...
journal_title:International journal of pediatric otorhinolaryngology
pub_type: 杂志文章
doi:10.1016/j.ijporl.2019.109809
更新日期:2020-03-01 00:00:00
abstract:OBJECTIVES:Bilateral BAHAs in adults with bilateral hearing loss (BHL) have proven to be superior to unilateral fitting, in both audiologically measurements and in overall patient satisfaction. There have been no similar studies in children. Furthermore, a recent meta-analysis of children with unilateral hearing loss (...
journal_title:International journal of pediatric otorhinolaryngology
pub_type: 杂志文章
doi:10.1016/j.ijporl.2006.09.014
更新日期:2007-01-01 00:00:00
abstract::Branchial cleft anomalies are congenital developmental defects that typically present as a soft fluctuant mass or fistulous tract along the anterior border of the sternocleidomastoid muscle. However, branchial anomalies can manifest atypically, presenting diagnostic and therapeutic challenges. Error or delay in diagno...
journal_title:International journal of pediatric otorhinolaryngology
pub_type: 杂志文章
doi:10.1016/s0165-5876(00)00355-4
更新日期:2000-08-31 00:00:00
abstract:OBJECTIVES:Nasal dilators are being developed for the purpose of increasing air flow in the nasal valve. The aim of this study is to evaluate the use of the internal nasal dilator (IND) by adolescent athletes. METHODS:A double-blind, crossover clinical trial in which we evaluated 54 adolescents using experimental and ...
journal_title:International journal of pediatric otorhinolaryngology
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.ijporl.2016.02.023
更新日期:2016-05-01 00:00:00
abstract::A total of 165 children from a school for the deaf in Malaysia were screened to find out the prevalence of additional conductive hearing loss. Otological examination, tympanometry and pure tone audiometry were performed in all these children. Fifty-one children (30.9%) had additional conductive hearing loss. Middle ea...
journal_title:International journal of pediatric otorhinolaryngology
pub_type: 杂志文章
doi:10.1016/0165-5876(94)90003-5
更新日期:1994-01-01 00:00:00
abstract::Formalin-fixed, paraffin-embedded and frozen tissues of 24 patients with primary nasopharyngeal angiofibroma, of whom seven had recurrences, were studied immunohistochemically for the expression of CD34 antigen using two different antibodies (HPCA-1 and QBEND 10). In all cases, there was an exclusive staining of endot...
journal_title:International journal of pediatric otorhinolaryngology
pub_type: 杂志文章
doi:10.1016/s0165-5876(98)00072-x
更新日期:1998-08-01 00:00:00
abstract:OBJECTIVES:To evaluate the prevalence of nocturnal enuresis (NE) in children diagnosed with obstructive sleep apnea syndrome (OSAS) and the effect of tonsillectomy and adenoidectomy on enuresis. DESIGN, SETTING, AND PARTICIPANTS:All children 4-18 years of age who underwent polysomnography (PSG) between January 2003 an...
journal_title:International journal of pediatric otorhinolaryngology
pub_type: 杂志文章
doi:10.1016/j.ijporl.2006.01.011
更新日期:2006-08-01 00:00:00
abstract:OBJECTIVES:To provide age-equivalent norms for a 500Hz logon evoked ABR obtained in a group of children ranging 40 weeks-4 years old and compare these 500Hz tonal norms to age-equivalent norms for click-evoked ABR. METHODS:Seventy-seven infants and children ranging from conceptional age of 40 weeks (term babies) to 4 ...
journal_title:International journal of pediatric otorhinolaryngology
pub_type: 杂志文章
doi:10.1016/j.ijporl.2007.01.016
更新日期:2007-05-01 00:00:00
abstract:BACKGROUND:Chronic otitis media (COM) is one of the most common childhood diseases. Its pathophysiology is complex and multifactorial. The role of specific mucin glycoprotein subtypes in OM is only recently being elucidated. OBJECTIVE:To determine the relationship between middle ear fluid mucins and clinical variables...
journal_title:International journal of pediatric otorhinolaryngology
pub_type: 杂志文章
doi:10.1016/j.ijporl.2016.09.009
更新日期:2016-11-01 00:00:00
abstract:INTRODUCTION:Culture-independent methods, based on bacterial 16 S rRNA gene sequencing, have been used previously to investigate the adenotonsillar microbiota. However, these studies have focused on a single sampling site (usually a surface swab). We aimed to investigate potential differences in adenotonsillar microbio...
journal_title:International journal of pediatric otorhinolaryngology
pub_type: 杂志文章
doi:10.1016/j.ijporl.2018.07.024
更新日期:2018-10-01 00:00:00
abstract:OBJECTIVE:The high frequency of risk factors detected within the newborn population increases the total number of children that should receive regular follow-ups. However, in some circumstances, this could be beyond the capacity of the health system. Therefore, careful interpretation and selection of risk factors, and ...
journal_title:International journal of pediatric otorhinolaryngology
pub_type: 杂志文章
doi:10.1016/j.ijporl.2013.10.052
更新日期:2014-02-01 00:00:00
abstract:OBJECTIVES:Cochlear implantation does wonders for children suffering from severe to profound hearing loss, especially when the child is less than 12 months of age. However, most studies indicate that detection and implantation are done well beyond that age, owing to poor socioeconomic status, parental education, and in...
journal_title:International journal of pediatric otorhinolaryngology
pub_type: 杂志文章
doi:10.1016/j.ijporl.2020.110156
更新日期:2020-09-01 00:00:00
abstract:OBJECTIVE:Phospholipase A2 (PLA2) plays a major part in growth regulation, differentiation and inflammation. It has been proposed as an evaluating marker for infection and inflammation. The aim of this study was to investigate activity of serum type II secretory PLA2 (sPLA2 IIa) in obstructive sleep apnea (OSA) and rec...
journal_title:International journal of pediatric otorhinolaryngology
pub_type: 杂志文章
doi:10.1016/j.ijporl.2011.12.026
更新日期:2012-04-01 00:00:00
abstract::Accidental ingestion of foreign bodies by children is not an uncommon event. Now a day, with increasing accessibility of electronic toys and devices to the children, ingestion of miniature size batteries is on the rise [J. Am. Med. Assoc., 249, (1983), 2495]. Unlike the usual foreign bodies, ingestion of button alkali...
journal_title:International journal of pediatric otorhinolaryngology
pub_type: 杂志文章
doi:10.1016/s0165-5876(01)00624-3
更新日期:2002-03-15 00:00:00
abstract:BACKGROUND:Prosody is the aspect of language that conveys emotion by changes in tone, rhythm, and emphasis during speech and the term specific language impairment (SLI) refers to children whose language development is substantially below their chronological age, despite a normal nonverbal intelligence and no obvious ne...
journal_title:International journal of pediatric otorhinolaryngology
pub_type: 杂志文章
doi:10.1016/j.ijporl.2014.11.001
更新日期:2015-01-01 00:00:00
abstract:OBJECTIVES:To objectively assess the effectiveness of endoscopy assisted adenoidectomy utilizing adenoid tissue volume measurement and to set some parameters for which patients are more legible to this procedure. METHODS:Forty three patients for whom adenoidectomy was conventionally done using adenoid curettes. Surgeo...
journal_title:International journal of pediatric otorhinolaryngology
pub_type: 杂志文章
doi:10.1016/j.ijporl.2014.04.031
更新日期:2014-08-01 00:00:00
abstract::The sternocleidomastoid tumor of infancy is an uncommon clinical entity which has received little attention in the otolaryngological literature. The diagnosis must be considered in any infant presenting with a lateral neck mass. Although its natural history favors spontaneous regression, the mass may initially grow in...
journal_title:International journal of pediatric otorhinolaryngology
pub_type: 杂志文章
doi:10.1016/0165-5876(87)90105-4
更新日期:1987-10-01 00:00:00
abstract::Second branchial cleft cysts and sinuses rarely present diagnostic problems to the pediatric otolaryngologist as their course is usually predictable based on consistent embryologic development. However, we evaluated two fistula tracts that did not fit the classic description of second branchial tract fistulas. Upon ra...
journal_title:International journal of pediatric otorhinolaryngology
pub_type: 杂志文章
doi:10.1016/s0165-5876(98)00077-9
更新日期:1998-11-15 00:00:00
abstract:OBJECTIVE:Identify differences in adenoidectomy performed by curettage versus electrocautery ablation. DESIGN:To receive adenoidectomy by curettage or electrocautery ablation 38 patients undergoing adenoidectomy or adenotonsillectomy were prospectively randomized. The study was completed by 24 patients. All tonsillect...
journal_title:International journal of pediatric otorhinolaryngology
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1016/s0165-5876(97)00159-6
更新日期:1998-03-01 00:00:00
abstract:OBJECTIVE:This study evaluated disease severity of children referred to a pediatric otolaryngology practice with a diagnosis of otitis media (OM) before and after tympanostomy tube (TT) placement. METHODS:A retrospective review was conducted of patients referred to a pediatric otolaryngology practice from January 2000...
journal_title:International journal of pediatric otorhinolaryngology
pub_type: 杂志文章
doi:10.1016/j.ijporl.2005.07.001
更新日期:2006-02-01 00:00:00
abstract:INTRODUCTION:Dexmedetomidine is a novel pharmacologic agent that has become a frequently used adjunct during care of pediatric patients with obstructive sleep apnea (OSA) undergoing tonsillectomy. While generally safe and effective, dexmedetomidine is associated with adverse effects of hypotension and bradycardia from ...
journal_title:International journal of pediatric otorhinolaryngology
pub_type: 杂志文章
doi:10.1016/j.ijporl.2017.11.025
更新日期:2018-01-01 00:00:00
abstract:OBJECTIVE:Antrochoanal polyp (ACP) is a polypoid lesion originating from the maxillary sinus, emerging from the ostium and extending to the choana. Although the etiologic causes of ACP are not known completely, it presents a fairly uniform clinical appearance. Nitric oxide (NO) has an important role in non-specific imm...
journal_title:International journal of pediatric otorhinolaryngology
pub_type: 杂志文章
doi:10.1016/s0165-5876(02)00405-6
更新日期:2003-04-01 00:00:00