Abstract：
:Since its inception a decade ago, sol-gel encapsulation has opened up an intriguing new way to immobilize biological materials. An array of substances, including catalytic antibodies, DNA, RNA, antigens, live bacterial, fungal, plant and animal cells and whole protozoa, have been encapsulated in silica, metal-oxide, organosiloxane and hybrid sol-gel polymers. The advantages of these 'living ceramics' might give them applications as optical and electrochemical sensors, diagnostic devices, catalysts, and even bioartificial organs. With rapid advances in sol-gel precursors, nanoengineered polymers, encapsulation protocols and fabrication methods, this technology promises to revolutionize bioimmobilization.
journal_name
Trends Biotechnoljournal_title
Trends in biotechnologyauthors
Gill I,Ballesteros Adoi
10.1016/s0167-7799(00)01457-8subject
Has Abstractpub_date
2000-07-01 00:00:00pages
282-96issue
7eissn
0167-7799issn
1879-3096pii
S0167-7799(00)01457-8journal_volume
18pub_type
杂志文章,评审abstract：:Discussions on research quality and reproducibility are appearing in the pages of scientific journals with heightened significance and gaining media attention. Many institutions have developed guidelines to address the topic of quality in basic research, but questions remain about how best to implement and monitor com...
journal_title：Trends in biotechnology
pub_type： 杂志文章
doi：10.1016/j.tibtech.2013.06.007
更新日期：2013-10-01 00:00:00
abstract：:Intravital imaging enables to study dynamic tumour-stroma interactions within primary and metastatic sites, including the lung. The combination of optical windows with specific molecular probes targeting the tumour microenvironment will provide new insights into prometastatic stromal interactions and lead to novel the...
journal_title：Trends in biotechnology
pub_type： 杂志文章
doi：10.1016/j.tibtech.2016.05.001
更新日期：2017-01-01 00:00:00
abstract：:Process analytical technology (PAT) for the manufacture of monoclonal antibodies (mAbs) is defined by an integrated set of advanced and automated methods that analyze the compositions and biophysical properties of cell culture fluids, cell-free product streams, and biotherapeutic molecules that are ultimately formulat...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.tibtech.2020.07.004
更新日期：2020-10-01 00:00:00
abstract：:Attempts through somatic cell nuclear transfer to expand wild populations that have shrunk to critical numbers is a logical extension of the successful cloning of mammals. However, although the first mammal was cloned 10 years ago, nuclear reprogramming remains phenomenological, with abnormal gene expression and epige...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.tibtech.2007.03.007
更新日期：2007-05-01 00:00:00
abstract：:Nanotechnology is currently driving the dental materials industry to substantial growth, thus reflecting on improvements in materials available for oral prevention and treatment. The present review discusses new developments in nanotechnology applied to dentistry, focusing on the use of nanomaterials for improving the...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.tibtech.2015.09.005
更新日期：2015-11-01 00:00:00
abstract：:The therapeutic and commercial success of monoclonal antibodies (mAbs) has inspired innovative approaches aimed at increasing their potency and broadening their applicability. Among these, cocktails of recombinant human mAbs are a logical next step because they combine the technological advances made in the field of a...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.tibtech.2007.07.005
更新日期：2007-09-01 00:00:00
abstract：:The expression 'crystal clear' stems from the science of crystallography, which determines the detailed atomic structures of materials by exposing crystals to X-rays. Protein structures are pivotal to the success of rational drug design and other biotechnology applications; however, obtaining high quality crystals pos...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.tibtech.2008.10.008
更新日期：2009-02-01 00:00:00
abstract：:The application of recombinant-DNA methods for the production of therapeutic proteins has, over the past decade, driven the development of new technology for the analysis and characterization of biological molecules. High performance capillary electrophoresis (HPCE) has generated enormous interest among biochemists, a...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/0167-7799(91)90078-v
更新日期：1991-07-01 00:00:00
abstract：:In recent years, entrapment of cells within spheres of Ca2+ alginate has become the most widely used technique for immobilizing living cells. This versatile method includes applications ranging from immobilization of living or dead cells in bioreactors, immobilization of plant protoplasts for micropropagation and immo...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/0167-7799(90)90139-o
更新日期：1990-03-01 00:00:00
abstract：:Enantiomerically pure cyanohydrins are important synthetic intermediates for pharmaceuticals and agrochemicals. They are produced by enzyme-catalysed synthesis using oxynitrilases. Sufficient quantities of enzyme are available via cheap natural sources and there have been recent advances in overexpression production o...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/s0167-7799(00)01452-9
更新日期：2000-06-01 00:00:00
abstract：:Microbial consortia have been used in biotechnology processes, including fermentation, waste treatment, and agriculture, for millennia. Today, synthetic biologists are increasingly engineering microbial consortia for diverse applications, including the bioproduction of medicines, biofuels, and biomaterials from inexpe...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.tibtech.2018.11.002
更新日期：2019-02-01 00:00:00
abstract：:Taxol® (generic name paclitaxel) represents one of the most clinically valuable natural products known to mankind in the recent past. More than two decades have elapsed since the notable discovery of the first Taxol®-producing endophytic fungus, which was followed by a plethora of reports on other endophytes possessin...
journal_title：Trends in biotechnology
pub_type： 杂志文章
doi：10.1016/j.tibtech.2014.03.011
更新日期：2014-06-01 00:00:00
abstract：:Vascularisation is key to developing large transplantable tissue constructs capable of providing therapeutic benefits. The vascularised tissue engineering chamber originates from surgical concepts in tissue prefabrication and microsurgery. It serves as an in vivo bioreactor in the form of a closed, protected space sur...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.tibtech.2018.05.009
更新日期：2018-10-01 00:00:00
abstract：:Marine natural products (NP) are unanimously acknowledged as the 'blue gold' in the urgent quest for new pharmaceuticals. Although corals are among the marine organisms with the greatest diversity of secondary metabolites, growing evidence suggest that their symbiotic bacteria produce most of these bioactive metabolit...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.tibtech.2013.06.004
更新日期：2013-10-01 00:00:00
abstract：:Lyases catalyse the cleavage of C-C, C-N, C-O and other bonds by elimination to produce double bonds or, conversely, catalyse the addition of groups to double bonds. These enzymes do not require cofactor recycling, show an absolute stereospecificity and can give a theoretical yield of 100%, compared with only 50% for ...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/0167-7799(94)90112-0
更新日期：1994-03-01 00:00:00
abstract：:Cyclodextrins, macrocyclic carbohydrates with apolar internal cavities, can form complexes with, and solubilize many normally water-insoluble compounds. Ferrocene and its derivatives, tetrathiafulvalene and tetramethylbenzidine, can function as redox mediators, but are insoluble in water; when they are complexed with ...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/S0167-7799(00)89002-2
更新日期：1995-11-01 00:00:00
abstract：:The endothelium lining blood vessels expresses molecules that are restricted in their expression to a particular tissue or organ. These molecules are attractive targets for therapy and diagnosis because they allow agents to be delivered specifically to the blood vessels supplying the desired tissue. However, it is dif...
journal_title：Trends in biotechnology
pub_type： 杂志文章
doi：10.1016/S0167-7799(03)00079-9
更新日期：2003-05-01 00:00:00
abstract：:Psychrophilic anaerobic treatment is an attractive option for wastewaters that are discharged at moderate to low temperature. The expanded granular sludge bed (EGSB) reactor has been shown to be a feasible system for anaerobic treatment of mainly soluble and pre-acidified wastewater at temperatures of 5--10 degrees C....
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/s0167-7799(01)01701-2
更新日期：2001-09-01 00:00:00
abstract：:The announcement that the European Union (EU) had reached an agreement allowing Member States (MS) to ban genetically modified (GM) crops confirms that the EU has chosen to ignore the food security challenge issued to the world by the Food and Agriculture Organization of the United Nations (FAO) in 2009. The FAO sugge...
journal_title：Trends in biotechnology
pub_type： 杂志文章
doi：10.1016/j.tibtech.2016.04.003
更新日期：2016-07-01 00:00:00
abstract：:Low production cost is a key factor driving the development of plants and plant tissue cultures for the synthesis of therapeutic and other foreign proteins. Because product yield and concentration exert a major influence on process economics, improving foreign protein accumulation is crucial for enhancing the commerci...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.tibtech.2006.06.012
更新日期：2006-09-01 00:00:00
abstract：:Building robust manufacturing processes from biological components is a task that is highly complex and requires sophisticated tools to describe processes, inputs, and measurements and administrate management of knowledge, data, and materials. We argue that for bioengineering to fully access biological potential, it w...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.tibtech.2015.11.006
更新日期：2016-03-01 00:00:00
abstract：:Allergenicity prediction is one of the most challenging aspects in the safety assessment of foods derived from either biotechnology or novel food proteins. Here we present a bottom-up strategy that defines a priori the specific risk assessment (RA) needs based on a database appropriately built for such purposes. ...
journal_title：Trends in biotechnology
pub_type： 杂志文章
doi：10.1016/j.tibtech.2020.05.011
更新日期：2021-01-01 00:00:00
abstract：:Here, we discuss recent advances in the development of artificial red blood cell (RBC) substitutes, illustrating lessons learned from initial attempts using perfluorocarbon (PFC) emulsions and acellular hemoglobin-based oxygen carriers (HBOCs). We also highlight novel oxygen-containing microparticles, nanoparticles, a...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.tibtech.2014.05.001
更新日期：2014-09-01 00:00:00
abstract：:In the wake of public distrust regarding biotechnology, it has been suggested that the debate should be moved "upstream", whereby the public help to set research priorities. Although many scientists see this as an illogical reaction to a loss of faith in science, we argue that the boundaries between science and its te...
journal_title：Trends in biotechnology
pub_type： 杂志文章
doi：10.1016/j.tibtech.2005.12.007
更新日期：2006-02-01 00:00:00
abstract：:Hydrophobic charge induction chromatography using 4-mercapto-ethyl-pyridine as the ligand is an effective method for the separation of antibodies from a variety of feedstocks. Antibodies are adsorbed in physiological conditions without preliminary concentration. Desorption occurs when the pH is lowered, thus inducing ...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/s0167-7799(02)01980-7
更新日期：2002-08-01 00:00:00
abstract：:One of the goals of synthetic biology is to build regulatory circuits that control cell behavior, for both basic research purposes and biomedical applications. The ability to build transcriptional regulatory devices depends on the availability of programmable, sequence-specific, and effective synthetic transcription f...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.tibtech.2015.12.014
更新日期：2016-07-01 00:00:00
abstract：:Atherosclerosis and heart disease are still the leading causes of morbidity and mortality worldwide. The lack of suitable autologous grafts has produced a need for artificial grafts but the patency of such grafts is limited compared to natural materials. Tissue engineering, whereby living tissue replacements can be co...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.tibtech.2005.06.006
更新日期：2005-09-01 00:00:00
abstract：:Proteins found in nature have traditionally been the most frequently used biocatalysts to produce numerous natural products ranging from commodity chemicals to pharmaceuticals. Protein engineering has emerged as a powerful biotechnological toolbox in the development of metabolic engineering, particularly for the biosy...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.tibtech.2019.12.008
更新日期：2020-07-01 00:00:00
abstract：:Advances in our capacity to design and use novel strategies for achieving inducible gene expression will improve our ability to define gene function. An extremely efficient system designed by nature -- that of the regulatable phytochrome system in plants -- has provided the basis for developing a novel inducible gene ...
journal_title：Trends in biotechnology
pub_type： 杂志文章
doi：10.1016/s0167-7799(02)00022-7
更新日期：2003-02-01 00:00:00
abstract：:Many of the demands in the production of microalgae at a technical scale cannot presently be met by state-of-the-art cultivation technologies based on suspensions. Immobilized cultivation using porous substrate bioreactors (PSBRs) is characterized by a reduction of liquid reaction volumes by several orders of magnitud...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.tibtech.2016.06.004
更新日期：2017-02-01 00:00:00
