Adenovirus E4orf4 protein interacts with both Balpha and B' subunits of protein phosphatase 2A, but E4orf4-induced apoptosis is mediated only by the interaction with Balpha.


:Adenovirus E4orf4 protein is a multifunctional viral regulator, which is involved in down regulation of virally-modulated signal transduction, in control of alternative splicing of viral mRNAs, and in induction of apoptosis in transformed cells. It has been previously shown that E4orf4 interacts with protein phosphatase 2A through the phosphatase Balpha subunit. It was further shown that PP2A is required for performing the various E4orf4 functions. We report here that E4orf4 interacts with multiple isoforms of the PP2A-B' subunit, as well as with Balpha. We map the interaction sites of the B subunits on E4orf4 and show that they overlap but are not identical. We identify a dominant negative E4orf4 mutant, which disrupts the PP2A holoenzyme. We show that induction of apoptosis by E4orf4, which we previously reported to require the interaction with Balpha, is not affected by the interaction with B'. Our results suggest that the interaction of E4orf4 with various PP2A subpopulations may mediate the different E4orf4 functions.






Shtrichman R,Sharf R,Kleinberger T




Has Abstract


2000-08-03 00:00:00














  • EGFRvIII gene rearrangement is an early event in glioblastoma tumorigenesis and expression defines a hierarchy modulated by epigenetic mechanisms.

    abstract::Amplification and rearrangements of the epidermal growth factor receptor (EGFR) gene are frequently found in glioblastoma multiforme (GBM). The most common variant is EGFR variant III (EGFRvIII). Research suggests that EGFRvIII could be a marker for a cancer stem cell or tumor-initiating population. If amplification a...


    pub_type: 杂志文章


    authors: Del Vecchio CA,Giacomini CP,Vogel H,Jensen KC,Florio T,Merlo A,Pollack JR,Wong AJ

    更新日期:2013-05-23 00:00:00

  • Phosphorylation of Ets1 regulates the complementation of a CSF-1 receptor impaired in mitogenesis.

    abstract::Ets1, the founder member of the Ets transcription factor family, is involved in a variety of developmental and cellular processes. Previous studies have shown that serine phosphorylation of Ets1 inhibits its DNA binding activity, suggesting that phosphorylation is important in the regulation of Ets1 function. To furth...


    pub_type: 杂志文章


    authors: Rabault B,Roussel MF,Quang CT,Ghysdael J

    更新日期:1996-08-15 00:00:00

  • Selective destruction of glioblastoma cells by interference with the activity or expression of ATF5.

    abstract::Glioblastoma multifome is the most common and most aggressive primary brain tumor with no current curative therapy. We found expression of the bZip transcription factor ATF5 in all 29 human glioblastomas and eight human and rat glioma cell lines assessed. ATF5 is not detectably expressed by mature brain neurons and as...


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    authors: Angelastro JM,Canoll PD,Kuo J,Weicker M,Costa A,Bruce JN,Greene LA

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  • Silencing of imprinted CDKN1C gene expression is associated with loss of CpG and histone H3 lysine 9 methylation at DMR-LIT1 in esophageal cancer.

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    authors: Soejima H,Nakagawachi T,Zhao W,Higashimoto K,Urano T,Matsukura S,Kitajima Y,Takeuchi M,Nakayama M,Oshimura M,Miyazaki K,Joh K,Mukai T

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  • DNA: leukemia's secret weapon of bone mass destruction.

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    pub_type: 评论,杂志文章


    authors: Tait S

    更新日期:2013-10-31 00:00:00

  • Overexpression of both catalytically active and -inactive cathepsin D by cancer cells enhances apoptosis-dependent chemo-sensitivity.

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    pub_type: 杂志文章


    authors: Beaujouin M,Baghdiguian S,Glondu-Lassis M,Berchem G,Liaudet-Coopman E

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  • Potent inhibitors of cyclin-dependent kinase 2 induce nuclear accumulation of wild-type p53 and nucleolar fragmentation in human untransformed and tumor-derived cells.

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    pub_type: 杂志文章


    authors: David-Pfeuty T

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  • MicroRNAs as potential cancer therapeutics.

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    pub_type: 杂志文章,评审


    authors: Trang P,Weidhaas JB,Slack FJ

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    authors: Mudduluru G,Ceppi P,Kumarswamy R,Scagliotti GV,Papotti M,Allgayer H

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    authors: Yoo MH,Song H,Woo CH,Kim H,Kim JH

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  • Males absent on the first (MOF): from flies to humans.

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    authors: Rea S,Xouri G,Akhtar A

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    authors: Stürzbecher HW,Brain R,Maimets T,Addison C,Rudge K,Jenkins JR

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  • The retinoblastoma protein modulates expression of genes coding for diverse classes of proteins including components of the extracellular matrix.

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    authors: Rohde M,Warthoe P,Gjetting T,Lukas J,Bartek J,Strauss M

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    authors: Jang JH

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  • Apolipoprotein C-1 maintains cell survival by preventing from apoptosis in pancreatic cancer cells.

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    pub_type: 杂志文章


    authors: Forrest A,Gabrielli B

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    authors: Hornigold N,Devlin J,Davies AM,Aveyard JS,Habuchi T,Knowles MA

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    authors: Lauring J,Cosgrove DP,Fontana S,Gustin JP,Konishi H,Abukhdeir AM,Garay JP,Mohseni M,Wang GM,Higgins MJ,Gorkin D,Reis M,Vogelstein B,Polyak K,Cowherd M,Buckhaults PJ,Park BH

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    pub_type: 杂志文章


    authors: Pribyl LJ,Watson DK,Schulz RA,Papas TS

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    authors: Shao J,Fujiwara T,Kadowaki Y,Fukazawa T,Waku T,Itoshima T,Yamatsuji T,Nishizaki M,Roth JA,Tanaka N

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    pub_type: 杂志文章


    authors: Hurley PJ,Wilsker D,Bunz F

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    pub_type: 杂志文章


    authors: Zirn B,Samans B,Spangenberg C,Graf N,Eilers M,Gessler M

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    pub_type: 杂志文章,评审


    authors: Lockshin RA,Zakeri Z

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    authors: Perrem K,Rayner J,Voss T,Sturzbecher H,Jackson P,Braithwaite A

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    pub_type: 杂志文章


    authors: Sharma SV,Agatsuma T,Nakano H

    更新日期:1998-05-01 00:00:00

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    pub_type: 杂志文章


    authors: Li Y,Clevenger CV,Minkovsky N,Kumar KG,Raghunath PN,Tomaszewski JE,Spiegelman VS,Fuchs SY

    更新日期:2006-03-23 00:00:00