Abstract：
BACKGROUND:Use of selective Clostridium difficile culture as a diagnostic method for C. difficile associated disease requires to prove the toxigenic ability of the isolates. Toxin B detection by cell culture assay after growing the microorganism in enriched broth is the standard method, but it delays the final diagnosis for 3-5 days. This study compares retrospectively four rapid techniques for detecting these toxigenic C. difficile strains. METHODS:106 clinical isolates of C. difficile (72 toxigenic and 34 non-toxigenic), these and 16 clinical strains of other species of Clostridium were investigated. The four methods were performed directly from colonies growing on solid agar. They were: a) cytotoxin detection in cell culture; b) two PCR amplifications of toxin A and toxin B, respectively, and c) toxin A detection by an immunoenzymatic method (VIDAS CDA2). All these procedures were completed within a normal working day. RESULTS:Only the 72 toxigenic C. difficile strains gave positive results by cell culture and PCR techniques (sensitivity and specificity: 100%). A total of 14 out of 49 toxigenic C. difficile strains showed negative results by the VIDAS assay in the first run, but all them were positive in repeated tests. CONCLUSIONS:Although all methods performed well, the cytotoxicity assay done directly on colonies growing in CCFA is a simple and rapid technique, and appears to be well-suited for use in laboratories with access to cell cultures.
journal_name
Enferm Infecc Microbiol Clinjournal_title
Enfermedades infecciosas y microbiologia clinicaauthors
García A,Pérez JL,Pulido A,Niubó J,Pérez P,Martín Rsubject
Has Abstractpub_date
2000-03-01 00:00:00pages
109-12issue
3eissn
0213-005Xissn
1578-1852journal_volume
18pub_type
杂志文章abstract：INTRODUCTION:The incidence of human immunodeficiency virus (HIV) and other sexually transmitted diseases increases in males who have sex with males (MSM), despite the knowledge on how to prevent them. To determine the mechanisms that are driving this lack of prevention is important to reverse the trend. PATIENTS AND M...
journal_title：Enfermedades infecciosas y microbiologia clinica
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abstract：BACKGROUND:Evidence-based medicine (EBM) analyses scientific evidence for the efficacy/effectiveness of health interventions. It aims to identify and implement available interventions that have proved to be the best. This concept is now soundly established for clinical interventions, but just breaking for prevention, p...
journal_title：Enfermedades infecciosas y microbiologia clinica
pub_type： 杂志文章,评审
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更新日期：1999-01-01 00:00:00
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journal_title：Enfermedades infecciosas y microbiologia clinica
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abstract：:N. meningitidis is a major cause of meningitis and septicemia and a major public health problem in many countries. The disease, that can be fulminant, has a high mortality and may cause serious sequelae, even in cases of apparently optimal medical treatment. Chemoprophylaxis may prevent secondary cases among those in ...
journal_title：Enfermedades infecciosas y microbiologia clinica
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更新日期：2015-04-01 00:00:00
abstract：:Sepsis is one of the main causes of mortality and morbidity in hospitals. Early detection of pathogens using nucleic acid-based techniques speeds diagnosis of bacteremia and/or fungemia, aids the rapid application of appropriate antibiotics, reduces the use of unnecessary antibiotics, and lowers mortality. Two commerc...
journal_title：Enfermedades infecciosas y microbiologia clinica
pub_type： 杂志文章,评审
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abstract：:Antimicrobial prophylaxis in surgery refers to a very brief course of an antimicrobial agent initiated just before the start of the procedure. The efficacy of antimicrobials to prevent postoperative infection at the site of surgery (incisional superficial, incisional deep, or organ/space infection) has been demonstrat...
journal_title：Enfermedades infecciosas y microbiologia clinica
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doi：
更新日期：2002-08-01 00:00:00
abstract：:We present here 6 patients with Fasciola hepatica infection, all from rural origin, and with the risk factors of eating wild watercress. The more common symptoms were: fever (83%), abdominal pain (100%), weight loss (83%) and generalized myalgia and joint pain (67%). All patients presented with an absolute eosinophil ...
journal_title：Enfermedades infecciosas y microbiologia clinica
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doi：
更新日期：1992-11-01 00:00:00
abstract：:Infections are one of the main complications that decisively affect the final outcome of transplants. Clinical microbiology laboratory has a key role in diagnosis, treatment and prevention of these complications. Centres with transplant programs must be technically supported with a well developed laboratory with speci...
journal_title：Enfermedades infecciosas y microbiologia clinica
pub_type： 杂志文章
doi：10.1016/j.eimc.2011.05.015
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abstract：:The imipenem and meropenem breakpoints for Enterobacteriaceae established by the Clinical and Laboratory Standards Institute (CLSI) are somewhat lower than those established by the European Committee on Antimicrobial Susceptibility Testing (EUCAST), but are identical for ertapenem and doripenem. The differences are pr...
journal_title：Enfermedades infecciosas y microbiologia clinica
pub_type： 杂志文章,评审
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journal_title：Enfermedades infecciosas y microbiologia clinica
pub_type： 杂志文章
doi：
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journal_title：Enfermedades infecciosas y microbiologia clinica
pub_type： 杂志文章
doi：
更新日期：1989-08-01 00:00:00
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journal_title：Enfermedades infecciosas y microbiologia clinica
pub_type： 杂志文章
doi：10.1016/j.eimc.2019.01.012
更新日期：2019-11-01 00:00:00
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journal_title：Enfermedades infecciosas y microbiologia clinica
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更新日期：2013-02-01 00:00:00
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journal_title：Enfermedades infecciosas y microbiologia clinica
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更新日期：2014-01-01 00:00:00
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journal_title：Enfermedades infecciosas y microbiologia clinica
pub_type： 杂志文章,多中心研究
doi：10.1016/j.eimc.2010.05.005
更新日期：2011-01-01 00:00:00
abstract：BACKGROUND:Toxoplasmosis is a disease of increasing incidence. Its laboratory diagnosis is difficult, specially in acute toxoplasmosis. The data obtained in experimental models attempting to distinguish between acute and chronic toxoplasmosis by the simultaneous study of four serological tests: IgM and IgG antibodies, ...
journal_title：Enfermedades infecciosas y microbiologia clinica
pub_type： 杂志文章
doi：
更新日期：1994-03-01 00:00:00
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journal_title：Enfermedades infecciosas y microbiologia clinica
pub_type： 杂志文章,多中心研究
doi：10.1016/j.eimc.2008.09.006
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abstract：:The uncontrolled progression of the AIDS epidemic has made the development of an efficacious human immunodeficiency virus (HIV) vaccine a major objective of scientific research. No effective preventive vaccine against HIV is currently available and sterilizing immunity has not yet been achieved in animal models. This ...
journal_title：Enfermedades infecciosas y microbiologia clinica
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更新日期：2005-07-01 00:00:00
abstract：:The epidemiological surveillance data for tuberculosis in Spain in 2015 is analysed in this report. This information was gathered through cases reported to the Red Nacional de Vigilancia Epidemiológica [National Epidemiological Surveillance Network]. In addition, the update of the treatment of the cases reported in 20...
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journal_title：Enfermedades infecciosas y microbiologia clinica
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doi：10.1157/13123261
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pub_type： 杂志文章,评审
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更新日期：2004-04-01 00:00:00
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journal_title：Enfermedades infecciosas y microbiologia clinica
pub_type： 杂志文章
doi：10.1016/s0213-005x(08)72692-2
更新日期：2008-04-01 00:00:00
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journal_title：Enfermedades infecciosas y microbiologia clinica
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更新日期：2005-05-01 00:00:00
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journal_title：Enfermedades infecciosas y microbiologia clinica
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doi：10.1016/j.eimc.2016.10.009
更新日期：2018-01-01 00:00:00
abstract：INTRODUCTION:Identification of yeasts is based on morphological, biochemical and nutritional characteristics, and using molecular methods. Matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry, a new method for the identification of microorganisms, has demonstrated to be very useful. ...
journal_title：Enfermedades infecciosas y microbiologia clinica
pub_type： 杂志文章
doi：10.1016/j.eimc.2014.10.003
更新日期：2015-06-01 00:00:00
abstract：INTRODUCTION:Our previously described multilocus sequence typing (MLST) scheme for Enterococcus faecalis has provided insight into the population structure and global epidemiology of this organism. Two high-risk complexes, CC2 and CC9, especially adapted to the hospital environment and widely distributed in Europe and ...
journal_title：Enfermedades infecciosas y microbiologia clinica
pub_type： 杂志文章,多中心研究
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更新日期：2007-10-01 00:00:00
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journal_title：Enfermedades infecciosas y microbiologia clinica
pub_type： 杂志文章,评审
doi：10.1016/S0213-005X(13)70139-3
更新日期：2013-06-01 00:00:00
abstract：INTRODUCTION:Burkholderia cepacia (B. cepacia) complex is composed of 20 phylogenetically closely related bacterial species. Some species have emerged as opportunistic pathogens in immunocompromised patients and are responsible for nosocomial outbreaks. The B. cepacia complex is a recognized respiratory pathogen in pat...
journal_title：Enfermedades infecciosas y microbiologia clinica
pub_type： 杂志文章,多中心研究
doi：10.1016/j.eimc.2017.09.002
更新日期：2018-01-01 00:00:00
abstract：BACKGROUND:Methicillin-resistant Staphylococcus aureus (MRSA) has become an important hospital-acquired pathogen, with transfer of the organism from a carrier or infected patient to uninfected patients by the hands or clothing of staff as the main mode of transmission. METHODS:Investigation of a cluster of new cases o...
journal_title：Enfermedades infecciosas y microbiologia clinica
pub_type： 杂志文章
doi：10.1016/j.eimc.2012.10.002
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abstract：:The use of intravascular devices in the usual and daily clinical practice, used as diagnostic or as therapeutic means, is a reality nowadays, present in all hospitals of our medium, and it is increasing. In the area of intensive care, it constitutes a routine and prolonged procedure, and its contribution within the ho...
journal_title：Enfermedades infecciosas y microbiologia clinica
pub_type： 杂志文章
doi：
更新日期：1997-10-01 00:00:00
