Synthesis, incorporation efficiency, and stability of disulfide bridged functional groups at RNA 5'-ends.

Abstract:

:Modified guanosine monophosphates have been employed to introduce various functional groups onto RNA 5'-ends. Applications of modified RNA 5'-ends include the generation of functionalized RNA libraries for in vitro selection of catalytic RNAs, the attachment of photoaffinity-tags for mapping RNA-protein interactions or active sites in catalytic RNAs, or the nonradioactive labeling of RNA molecules with fluorescent groups. While in these and in similar applications a stable linkage is desired, in selection experiments for generating novel catalytic RNAs it is often advantageous that a functional group is introduced reversibly. Here we give a quantitative comparison of the different strategies that can be applied to reversibly attach functional groups via disulfide bonds to RNA 5'-ends. We report the preparation of functional groups with disulfide linkages, their incorporation efficiency into an RNA library, and their stability under various conditions.

journal_name

Bioorg Med Chem

authors

Sengle G,Jenne A,Arora PS,Seelig B,Nowick JS,Jäschke A,Famulok M

doi

10.1016/s0968-0896(00)00080-8

subject

Has Abstract

pub_date

2000-06-01 00:00:00

pages

1317-29

issue

6

eissn

0968-0896

issn

1464-3391

pii

S0968-0896(00)00080-8

journal_volume

8

pub_type

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