Electrophysiological characterization of human keratinocytes using the patch-clamp technique.

abstract：:We have recently demonstrated that telomerase activity is increased and telomere length shortened in lymphocytes from peripheral blood of patients with cutaneous T-cell lymphoma. In order to determine which cell type has increased telomerase activity and shortened telomere length, CD4+, CD8+, CLA+ CD3+ and CLA- CD3+ T...

journal_title：Experimental dermatology

authors： Wu KD,Hansen ER

更新日期：2001-10-01 00:00:00

abstract：:Leukotrienes constitute a group of lipid mediators, which may be subdivided into two groups, with leukotriene B4 on the one hand and cysteinyl leukotrienes on the other. Although leukotrienes are abundantly expressed in skin affected by diverse chronic inflammatory diseases, including atopic dermatitis, psoriasis, pem...

journal_title：Experimental dermatology

authors： Sadik CD,Sezin T,Kim ND

更新日期：2013-11-01 00:00:00

abstract：BACKGROUND:Methylenetetrahydrofolate reductase (MTHFR) is involved in the formation of methyl donors, which contribute to DNA methylation. DNA methylation is an essential epigenetic feature playing a critical role in gene regulation and cellular differentiation. In addition, MTHFR activity affects plasma homocysteine l...

journal_title：Experimental dermatology

authors： Weger W,Hofer A,Stanger O,Wolf P,El-Shabrawi Y,Renner W,Kerl H,Salmhofer W

更新日期：2008-09-01 00:00:00

abstract：:In this study, we investigated the process of X-ray-induced apoptosis of skin keratinocyte, and the functional role of protein kinase C delta (PKCdelta) and downstream signalling cascade. High-dose X-ray irradiation (10 Gy) led to the apoptosis of HaCaT keratinocyte, accompanied by PKCdelta cleavage. Treatment with PK...

journal_title：Experimental dermatology

authors： Lee YS,Sohn KC,Kim KH,Cho MJ,Hur GM,Yoon TJ,Kim SK,Lee K,Lee JH,Kim CD

更新日期：2009-01-01 00:00:00

abstract：:Lipoid proteinosis (OMIM 247100), also known as Urbach-Wiethe disease or hyalinosis cutis et mucosae, is a rare autosomal recessive disorder characterized by generalized thickening and scarring of the skin and mucosae. In 2002, the disorder was mapped to a locus on chromosome 1q21 and pathogenic mutations were identif...

journal_title：Experimental dermatology

authors： Chan I,Liu L,Hamada T,Sethuraman G,McGrath JA

更新日期：2007-11-01 00:00:00

abstract：:All species have evolved mechanisms of repair to restore tissue function following injury. Skin scarring is an inevitable and permanent endpoint for many postnatal organisms except for non-amniote vertebrates such as amphibians, which are capable of tissue regeneration. Furthermore, mammalian foetuses through mid-gest...

journal_title：Experimental dermatology

authors： Ud-Din S,Volk SW,Bayat A

更新日期：2014-09-01 00:00:00

abstract：:Tyrosine kinase inhibitors of the tyrphostin family which block EGF receptor kinase are reported to arrest the growth of psoriatic keratinocytes in vitro. Three tyrphostins with the potency ratio AG555 > AG18 > AG814 were found to arrest growth with no adverse cytotoxic effects. The potency ratio to inhibit keratinocy...

journal_title：Experimental dermatology

authors： Ben-Bassat H,Vardi DV,Gazit A,Klaus SN,Chaouat M,Hartzstark Z,Levitzki A

更新日期：1995-04-01 00:00:00

abstract：:In 2018, Schneider and Zouboulis analysed the available tools for studying sebaceous gland pathophysiology in vitro. Since then, the interest in this field remains unbroken, as demonstrated by recent reviews on sebaceous gland physiology, endocrinology and neurobiology, the role of sebaceous glands beyond acne, and se...

journal_title：Experimental dermatology

authors： Zouboulis CC,Yoshida GJ,Wu Y,Xia L,Schneider MR

更新日期：2020-09-02 00:00:00

abstract：:Microsatellite instability and reduced expression of mismatch repair proteins were reported in melanomas. However, little is known about mutational changes of the mismatch repair genes in radial growth-phase melanoma especially following UVB irradiation. To investigate these changes, an in vitro system consisting of r...

journal_title：Experimental dermatology

authors： Hussein MR,Wood GS

更新日期：2003-12-01 00:00:00

abstract：:Hair follicle heterogeneity may be regulated by distinct dermal papillae (DP) that represent mesenchymal lineages, which can be defined by Sox2 expression. However, it was recently shown that GFP expression in the Sox2: GFP+/- mouse model occurs in the DPs of all hair follicle types, challenging the idea that hair fol...

journal_title：Experimental dermatology

authors： Salz L,Driskell RR

更新日期：2017-11-01 00:00:00

abstract：:Tropisetron is a serotonin receptor (5-HT-R)-modulating agent and approved as an antiemetic for patients undergoing chemotherapy. In the gut, it acts via specific serotonin receptors, 5-HT3 -R, to elicit its beneficial effects against nausea. We investigated whether tropisetron can affect inflammatory cell responses o...

journal_title：Experimental dermatology

authors： Stegemann A,Böhm M

更新日期：2019-03-01 00:00:00

abstract：:The present study assessed the molecular mechanism underlying ultraviolet (UV) B radiation-induced inhibition of the expression of the adhesion molecule ICAM-1 in human antigen-presenting cells (APC). UVB radiation-induced inhibition of ICAM-I expression in human peripheral blood monocytes was associated with the gene...

journal_title：Experimental dermatology

authors： Grewe M,Stege H,Vink A,Klammer M,Ruzicka T,Roza L,Krutmann J

更新日期：2000-12-01 00:00:00

abstract：:Tissue-invasive T cells are observed in many inflammatory dermatological diseases, but in most cases, it is not known how they were attracted, what they might recognize, and to which extent they are activated. Answering these questions is surely essential for understanding pathogeneses of the diseases. In a recent iss...

journal_title：Experimental dermatology

authors： Dornmair K

更新日期：2014-06-01 00:00:00

abstract：:Epidermal keratinocytes undergo a continuous process of terminal differentiation, which is accompanied by a dramatic change in the expression and composition of keratins. This complex and carefully orchestrated process is regulated by a large number of signal transduction events and transcriptional factors as well as ...

journal_title：Experimental dermatology

authors： Wang M,Yue Z,Paus R,Ramot Y

更新日期：2014-09-01 00:00:00

abstract：:Chemokines are small chemotactic proteins that have a crucial role in leukocyte recruitment into tissue. Targeting these mediators has been suggested as a potential therapeutic option in inflammatory skin diseases such as psoriasis. Using quantitative RT-PCR, we found CCL7, a chemokine ligand known to interact with mu...

journal_title：Experimental dermatology

authors： Brunner PM,Glitzner E,Reininger B,Klein I,Stary G,Mildner M,Uhrin P,Sibilia M,Stingl G

更新日期：2015-07-01 00:00:00

abstract：:Topical photodynamic therapy with 5-aminolevulinic acid (5-ALA) is a newly established treatment modality for epithelial skin cancer. Skin distribution of different 20% 5-ALA formulations was investigated by detection of protoporphyrin IX (PpIX) using an ex vivo porcine skin model. Fluorescence of PpIX was first detec...

journal_title：Experimental dermatology

authors： Maisch T,Worlicek C,Babilas P,Landthaler M,Szeimies RM

更新日期：2008-10-01 00:00:00

abstract：:Primary hyperhidrosis is characterized by excessive sweating in palmar, plantar and axillary body regions. Gland hypertrophy and the existence of a third type of sweat gland, the apoeccrine gland, with high fluid transporting capabilities have been suggested as possible causes. This study investigated whether sweat gl...

journal_title：Experimental dermatology

authors： Bovell DL,MacDonald A,Meyer BA,Corbett AD,MacLaren WM,Holmes SL,Harker M

更新日期：2011-12-01 00:00:00

abstract：:Patients belonging to xeroderma pigmentosum (XP) complementation group C comprise one-third of all XP patients. Only four major reports compiled larger groups of XP-C patients from southern Europe (12 pts), North America (16 pts) and Africa (14 and 56 pts) as well as their genetic background (46 XPC mutations). We ide...

journal_title：Experimental dermatology

authors： Schäfer A,Hofmann L,Gratchev A,Laspe P,Schubert S,Schürer A,Ohlenbusch A,Tzvetkov M,Hallermann C,Reichrath J,Schön MP,Emmert S

更新日期：2013-01-01 00:00:00

abstract：:NC/Nga mice are known to develop scratching dermatitis akin to atopic dermatitis, under conventional (Conv), but not under the specific-pathogen-free (SPF) condition. In this study, we examined the effects of mechanical-scratching on the spontaneous scratching counts (sign of itching), in relation to the cutaneous pro...

journal_title：Experimental dermatology

authors： Takaoka A,Arai I,Sugimoto M,Futaki N,Sakurai T,Honma Y,Nakaike S

更新日期：2007-04-01 00:00:00

abstract：:The ethanolic extract of Resina Draconis (RDEE) has been reported beneficial to normal wound healing yielding more regularly arranged collagen fibres. Loureirin B, a major component in RDEE, has been supposed to be effective on the prevention and treatment of pathological scars. To investigate the therapeutic effects ...

journal_title：Experimental dermatology

authors： Bai X,He T,Liu J,Wang Y,Fan L,Tao K,Shi J,Tang C,Su L,Hu D

更新日期：2015-05-01 00:00:00

abstract：:DECORIN is a prototypical member of the small leucine-rich proteoglycan (SLRP) family that plays important roles in numerous biological processes and cellular biological pathways. We previously showed that Decorin expression was highly enhanced in mouse dorsal hair follicles (HFs) during the anagen phase and was reduc...

journal_title：Experimental dermatology

authors： Zhou L,Jing J,Wang H,Wu X,Lu Z

更新日期：2018-11-01 00:00:00

abstract：:Lipoxygenases (LOX) and cyclooxygenase (COX) are the main enzymes for PUFA metabolism to highly bio-active prostaglandins, leukotrienes, thromboxanes, lipoxins, resolvins and protectins. LOX and COX pathways are important for the regulation of pro-inflammatory or pro-resolving metabolite synthesis and metabolism for v...

journal_title：Experimental dermatology

authors： Töröcsik D,Weise C,Gericke J,Szegedi A,Lucas R,Mihaly J,Worm M,Rühl R

更新日期：2019-02-01 00:00:00

abstract：:Despite groundbreaking new treatments such as checkpoint inhibition and targeted therapy, the overall response and survival rates are limited in patients with metastatic melanoma. Here, we hypothesize that melatonin and its metabolites could be promising boosters of the efficacy of BRAF/MEK inhibitors in patients with...

journal_title：Experimental dermatology

authors： Kleszczyński K,Böhm M

更新日期：2020-09-01 00:00:00

abstract：:Current gold standard lower extremity cutaneous wound management is not always effective. Cutaneous wounds generate a "current of injury" which is directly involved in wound healing processes. Application of exogenous electrical stimulation has been hypothesised to imitate the natural electric current that occurs in c...

journal_title：Experimental dermatology

authors： Ashrafi M,Alonso-Rasgado T,Baguneid M,Bayat A

更新日期：2017-02-01 00:00:00

abstract：:Patch-clamp recordings were carried out in the inside-out configuration in human keratinocytes of the cell line HaCaT. Patch pipettes were filled with 150 mM KCl, 1 mM CaCl(2) and 10 mM HEPES. In symmetrical KCl solutions, single channel currents from a large conductance channel (about 170 pS) were measured. Replaceme...

journal_title：Experimental dermatology

authors： Nguyen VH,Markwardt F

更新日期：2002-08-01 00:00:00

abstract：:There have been few reports of successful long-term culture of cells established from cutaneous basal cell carcinoma (BCC) tumors. Here, we describe techniques that have enabled us to establish three long-term cultures of BCC cells isolated from BCC tumors that arose in irradiated Patched 1 (Ptch1)(+/-) mice. All thre...

journal_title：Experimental dermatology

authors： So PL,Langston AW,Daniallinia N,Hebert JL,Fujimoto MA,Khaimskiy Y,Aszterbaum M,Epstein EH Jr

更新日期：2006-09-01 00:00:00

abstract：:To determine the therapeutic mechanism of PUVA in psoriasis vulgaris, the effects of PUVA on activated T lymphocytes were investigated in vitro. Peripheral blood mononuclear cells (PBMC) obtained from healthy volunteers were activated with Con A stimulation (Con A blasts). Both untreated PBMC and Con A blasts were irr...

journal_title：Experimental dermatology

authors： Urano K,Matsuyama T,Urano R,Matsuo I,Habu S

更新日期：1995-02-01 00:00:00

abstract：:Keloid is a dermal proliferative disorder characterized by the excessive proliferation and migration of keratinocytes and fibroblasts. Over-activation of the serine/threonine protein kinase, mammalian target of rapamycin (mTOR), plays a pivotal role in the process. Here, we show that both mTOR complex 1 (mTORC1) and m...

journal_title：Experimental dermatology

authors： Chen J,Liu K,Liu Y,Wang X,Zhang Z

更新日期：2019-03-01 00:00:00

abstract：:Calcium-mediated signals play important roles in epidermal barrier formation, skin homoeostasis and wound repair. Calmodulin 4 (Calm4) is a small, Ca2+ -binding protein with strong expression in suprabasal keratinocytes. In mice, Calm4 first appears in the skin at the time of barrier formation, and its expression incr...

journal_title：Experimental dermatology

authors： Lessard JC,Kalinin A,Bible PW,Morasso MI

更新日期：2015-01-01 00:00:00

abstract：:In systemic scleroderma (SSc) excessive deposition of collagen leads to fibrosis of various tissues including the skin. Previous studies have demonstrated that scleroderma fibroblasts in explant monolayer cultures are heterogeneous with respect to their levels of collagen synthesis. The critical role played by the ext...

journal_title：Experimental dermatology

authors： Mauch C,Kozlowska E,Eckes B,Krieg T

更新日期：1992-11-01 00:00:00