Abstract:
:To develop an effective hybrid bioartificial liver (BAL) device, the material of the scaffold is very important to support hepatocytes that have both growth ability and hepatic differentiated functions. In this study we used paper (Kimwipe, Kimberly-Clark Corp., Roswell, GA, U.S.A.) as a scaffold. Primary hepatocytes isolated from a normal adult rat liver could proliferate on the paper. The secretion of albumin into culture medium by the cells on the paper increased with time in culture and, compared to the cells on dishes, the amount of 48 h albumin secretion at Day 10 was two times larger. Perpendicular sections of hepatocytes on the paper revealed that the cells fell into cavities made by intersecting fibers, piled up, and formed three to four layers. The piled-up cells changed their shape from flat to cuboidal and enlarged their cytoplasm, which was rich in organelles such as mitochondria and peroxisomes with a nucleoid. In addition, they formed bile canalicular structures between the cells. Their morphological appearance was similar to in vivo hepatocytes. Paper (Kimwipe) may be a good candidate as a scaffold to make a BAL device.
journal_name
Artif Organsjournal_title
Artificial organsauthors
Mizuguchi T,Mitaka T,Sato F,Mochizuki Y,Hirata Kdoi
10.1046/j.1525-1594.2000.06506.xsubject
Has Abstractpub_date
2000-04-01 00:00:00pages
271-7issue
4eissn
0160-564Xissn
1525-1594pii
aor6506journal_volume
24pub_type
杂志文章abstract::Heparin remains the predominant anticoagulant during extracorporeal membrane oxygenation (ECMO). Heparin acts by potentiating the anticoagulant effect of antithrombin (ATIII). Acquired ATIII deficiency, common in pediatric patients requiring ECMO, may result in ineffective anticoagulation with heparin. ATIII replaceme...
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journal_title:Artificial organs
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journal_title:Artificial organs
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journal_title:Artificial organs
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pub_type: 杂志文章,评审
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journal_title:Artificial organs
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journal_title:Artificial organs
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更新日期:2000-10-01 00:00:00
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更新日期:1995-07-01 00:00:00
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pub_type: 杂志文章
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更新日期:1979-02-01 00:00:00
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pub_type: 杂志文章
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更新日期:1997-05-01 00:00:00
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pub_type: 杂志文章,多中心研究
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journal_title:Artificial organs
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journal_title:Artificial organs
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journal_title:Artificial organs
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