Abstract：
:It has been suggested that the increase in C3 and C4 levels in jejunal perfusates of patients with Crohn's disease (CD) results from local intestinal synthesis of complement. The present study evaluated the expression of these complement genes in inflamed tissues from patients with CD. Surgically resected specimens from patients with CD and control tissue obtained from subjects with adenocarcinoma of the colon were evaluated for C3 and C4 gene expression by the use of 35S-labelled anti-sense RNA probes. All tissue samples, diseased and normal tissue, expressed C4 mRNA throughout in the intestinal epithelium. C3 mRNA was not detected in epithelial cells in histologically normal tissue, but in diseased specimens there was a focal distribution of C3 mRNA in epithelial cells of the crypts, but not in villous epithelium. Focal C3 gene expression correlated with crypt abscess formation and the presence of polymorphonuclear leucocytes in the lumen of the crypts. In addition, C3 mRNA was also found in macrophages of the submucosa. These macrophages were CD68+, fusiform with faint cytoplasm and morphologically different from the large rounded lamina propria macrophages, which do not express C3 mRNA. Multinucleated giant cells did not express either C3 or C4 genes. In addition to its presence in intestinal epithelium, C4 mRNA was also expressed in mast cells, which however did not express C3 mRNA. These observations identify cells in the intestinal wall expressing complement genes and support the hypothesis that there is local regulated production of complement in the intestine of patients with CD, and subsequent complement activation may contribute to the inflammatory process.
journal_name
Clin Exp Immunoljournal_title
Clinical and experimental immunologyauthors
Laufer J,Oren R,Goldberg I,Horwitz A,Kopolovic J,Chowers Y,Passwell JHdoi
10.1046/j.1365-2249.2000.01168.xsubject
Has Abstractpub_date
2000-04-01 00:00:00pages
30-7issue
1eissn
0009-9104issn
1365-2249pii
cei1168journal_volume
120pub_type
杂志文章abstract：:Tolerogenic dendritic cells (DCs) play a critical role in the induction of regulatory T cells (Tregs ), which in turn suppress effector T cell responses. We have previously shown the induction of DCs from human and mouse monocytic cell lines, mouse splenocytes and human peripheral blood monocytes by a novel apolipopro...
journal_title：Clinical and experimental immunology
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journal_title：Clinical and experimental immunology
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journal_title：Clinical and experimental immunology
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doi：
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journal_title：Clinical and experimental immunology
pub_type： 杂志文章
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journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1981-10-01 00:00:00
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journal_title：Clinical and experimental immunology
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journal_title：Clinical and experimental immunology
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journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1982-09-01 00:00:00
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journal_title：Clinical and experimental immunology
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doi：
更新日期：1984-08-01 00:00:00
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journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1987-08-01 00:00:00
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journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1986-11-01 00:00:00
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journal_title：Clinical and experimental immunology
pub_type： 杂志文章
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journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
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journal_title：Clinical and experimental immunology
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doi：
更新日期：1981-12-01 00:00:00
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journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1982-07-01 00:00:00
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journal_title：Clinical and experimental immunology
pub_type： 杂志文章
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更新日期：1998-05-01 00:00:00
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journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1983-09-01 00:00:00
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journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1987-10-01 00:00:00
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journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1976-01-01 00:00:00
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journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1982-06-01 00:00:00
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journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2249.1995.tb03779.x
更新日期：1995-11-01 00:00:00
