Beneficial effect of HEPES buffer in repeated coronary reperfusion.

Abstract:

BACKGROUND:We have demonstrated previously that, when repeated reperfusion is performed after reocclusion, there is a decrease in the amount of myocardial salvage, despite early reperfusion. Treatment with nisoldipine induced a beneficial effect by reduction of infarct size in this experimental model. OBJECTIVE:To study the effect of HEPES buffer on infarct size, using a repeated-reperfusion model. METHODS:The left anterior descending coronary artery was occluded in anesthetized dogs. Thirty minutes after the occlusion, dogs were allocated randomly to either the treatment group (n = 7; HEPES 0.64 mmol/l infused intravenously throughout the experiment) or the control group (n = 8; saline). Occlusion was maintained for 2 h, followed by 1 h of reperfusion, then 1 h of reocclusion and 2 h of second reperfusion. An in-vivo area at risk was determined by gentian violet staining, and infarct size was defined and quantitated by triphenyl-tetrazolium chloride staining. RESULTS:Hemodynamic measurements were similar in both groups. Mass of necrosis/mass at risk was significantly smaller in the HEPES group (30.7 +/- 1.6%, mean +/- SEM) compared with controls (50.6 +/- 3.8%, P < 0.001). CONCLUSION:Treatment with HEPES induces a beneficial effect by reduction of infarct size in repeated coronary reperfusion.

journal_name

Coron Artery Dis

journal_title

Coronary artery disease

authors

Hammerman H,Ramadan R,Hir J,Moscovitz M

doi

10.1097/00019501-200003000-00013

subject

Has Abstract

pub_date

2000-03-01 00:00:00

pages

179-82

issue

2

eissn

0954-6928

issn

1473-5830

journal_volume

11

pub_type

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