当前位置： SCI文献检索 > AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY期刊下所有文献 > Ethanol exposure induces oxidative stress and impairs nitric oxide availability in the human placental villi: a possible mechanism of toxicity.

Ethanol exposure induces oxidative stress and impairs nitric oxide availability in the human placental villi: a possible mechanism of toxicity.

Abstract：

OBJECTIVE:We undertook this investigation to explore the effects of ethanol exposure on nitric oxide synthase levels and nitric oxide release. Our hypothesis was that ethanol exposure modifies nitric oxide activity within the placenta as a result of oxidative stress. STUDY DESIGN:Four 10-g samples of term normal human placental villous tissue were perifused with nonrecirculating Dulbecco's modified Eagle's medium and 25-mmol/L N-[2-hydroxyethyl]piperazine-N'-[2-ethanesulfonic acid] with 0-, 50-, 100-, or 200-mmol/L ethanol. After 2 hours of exposure, tissue was removed, fixed, and frozen for analysis. Immunohistochemical analysis was performed for subtype I or neuronal nitric oxide synthase (nNOS), subtype II or inducible nitric oxide synthase (iNOS), and subtype III or endothelial nitric oxide synthase (eNOS) localization. Western blot analysis was performed for eNOS quantitation. Cyclic guanosine monophosphate and copper-zinc superoxide dismutase levels were measured by electroimmunoassay and kinetic assay, respectively. Nitric oxide release was analyzed by a Sievers nitric oxide analyzer. RESULTS:Immunohistochemical examination confirmed that only eNOS was localized to the syncytiotrophoblasts. After ethanol exposure, eNOS protein expression increased 2.5- to 3.0-fold over that of the control. Tissue cyclic guanosine monophosphate content and nitric oxide release into the effluent were decreased, whereas superoxide dismutase levels were increased at higher ethanol levels (P <.05). CONCLUSION:Ethanol exposure appears to induce oxidative stress, which may account for the decreased nitric oxide release, because nitric oxide may be shunted toward scavenging free radicals. Increased eNOS protein expression may be a response to the increased demand for nitric oxide. Decreased nitric oxide availability could adversely affect placental blood flow regulation, which could, in turn, account for the growth restriction seen in ethanol-exposed fetuses.

journal_name

Am J Obstet Gynecol

journal_title

American journal of obstetrics and gynecology

authors

Kay HH,Grindle KM,Magness RR

doi

10.1067/mob.2000.104201

subject

Has Abstract

pub_date

2000-03-01 00:00:00

pages

682-8

issue

3

eissn

0002-9378

issn

1097-6868

pii

S0002-9378(00)80037-X

journal_volume

182

pub_type

杂志文章

相关文献

AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY文献大全
  • Cesarean section versus vaginal delivery for the breech fetus weighing less than 1,500 grams.

    abstract：:Although previous studies have suggested that the breech infant weighing less than 1,500 gm benefits from cesarean section, the published results have not demonstrated statistically significant differences. We have evaluated pediatric outcome data for 240 breech infants and 525 vertex infants who weighed less than 1,5...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 杂志文章

    doi：10.1016/0002-9378(83)90807-4

    authors： Main DM,Main EK,Maurer MM

    更新日期：1983-07-01 00:00:00

  • Repair of old complete perineal lacerations.

    abstract：:Our subjects were 32 patients whose old complete perineal lacerations were repaired in the 10 hospitals in the South Hampton Roads Metropolitan area over a 20-year period. The layer technique of repair (26 patients) and the Warren flap procedure (6 patients) were used. Function improved in 24 of 26 patients (92%) with...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 杂志文章

    doi：10.1016/s0002-9378(88)80053-x

    authors： Given FT Jr,Browning G

    更新日期：1988-09-01 00:00:00

  • Surgical-site infection in gynecologic surgery: pathophysiology and prevention.

    abstract：:Surgical-site infections (SSIs) represent a well-known cause of patient morbidity as well as added health care costs. In gynecologic surgery, particularly hysterectomy, SSIs are often the result of a number of risk factors that may or may not be modifiable. As both the Centers for Medicaid and Medicare Services and th...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 杂志文章,评审

    doi：10.1016/j.ajog.2017.02.014

    authors： Steiner HL,Strand EA

    更新日期：2017-08-01 00:00:00

  • Impact of menopause on collagen subtypes in the arcus tendineous fasciae pelvis.

    abstract：OBJECTIVE:The arcus tendineous fasciae pelvis (ATFP) provides support to the anterior vagina. The objective of this study was to determine the impact of menopause on the structural components of the ATFP. STUDY DESIGN:Biopsy specimens of the ATFP were obtained from 10 premenopausal, 5 postmenopausal, and 12 postmenopa...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 杂志文章

    doi：10.1016/j.ajog.2003.08.040

    authors： Moalli PA,Talarico LC,Sung VW,Klingensmith WL,Shand SH,Meyn LA,Watkins SC

    更新日期：2004-03-01 00:00:00

  • The effects of lymphokines and monokines on human sperm fertilizing ability in the zona-free hamster egg penetration test.

    abstract：:Antisperm cell-mediated immunity has been associated with infertility in men and women. The purpose of this study was to determine whether the products of activated lymphocytes and macrophages (lymphokines and monokines) affect the fertilizing ability of human spermatozoa in the zona-free hamster egg penetration test....

    journal_title：American journal of obstetrics and gynecology

    pub_type： 杂志文章

    doi：10.1016/0002-9378(89)90179-8

    authors： Hill JA,Cohen J,Anderson DJ

    更新日期：1989-05-01 00:00:00

  • Calculated capillary hydrostatic pressure in normal pregnancy and preeclampsia.

    abstract：:Capillary hydrostatic pressure has been calculated in normal pregnancy and preeclampsia. In humans, capillary hydrostatic pressure cannot be measured directly but may be calculated when the colloid osmotic pressure in plasma and interstitial fluid and interstitial fluid hydrostatic pressure are known (Starling equatio...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 杂志文章

    doi：10.1016/s0002-9378(87)80355-1

    authors： Oian P,Maltau JM

    更新日期：1987-07-01 00:00:00

  • Fetal acidosis in prolonged pregnancy cannot be attributed to cord compression alone.

    abstract：:Fetal heart rate abnormalities associated with prolonged pregnancy have been attributed to umbilical cord vulnerability rather than placental insufficiency. Although intrapartum fetal heart rate patterns indicative of umbilical cord compression are common beyond 41 weeks' gestation, fetal intolerance to labor develops...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 杂志文章

    doi：10.1016/s0002-9378(88)80031-0

    authors： Silver RK,Dooley SL,MacGregor SN,Depp R

    更新日期：1988-09-01 00:00:00

  • Coronavirus disease 2019 during pregnancy: a systematic review of reported cases.

    abstract：OBJECTIVE:This study aimed to conduct a systematic review of the clinical outcomes reported for pregnant patients with coronavirus disease 2019. DATA SOURCES:The PubMed, CINAHL, and Scopus databases were searched using a combination of key words such as "Coronavirus and/or pregnancy," "COVID and/or pregnancy," "COVID ...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 杂志文章

    doi：10.1016/j.ajog.2020.04.013

    authors： Della Gatta AN,Rizzo R,Pilu G,Simonazzi G

    更新日期：2020-07-01 00:00:00

  • The prevention of postoperative pelvic adhesions: an animal study comparing barrier methods with dextran 70.

    abstract：:There are two major approaches to the prophylaxis for adhesions: chemical methods (e.g., dextran) and barrier methods. Theoretically, dextran prevents the formation of adhesions by a surface-coating or hydroflotation effect, whereas the barrier methods either prevent the formation of or mask adhesions by covering and ...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 杂志文章

    doi：10.1016/0002-9378(82)90018-7

    authors： Soules MR,Dennis L,Bosarge A,Moore DE

    更新日期：1982-08-01 00:00:00

  • A regressive Meigs syndrome after definitive adnexal torsion.

    abstract：:The Meigs syndrome is a rare but well known syndrome in which removal of the tumor results in cure. We report a case of a regressive Meigs syndrome after a definitive adnexal torsion which highlights the major role of the vascular phenomena in the physiopathology of this puzzling syndrome. ...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 杂志文章

    doi：10.1016/j.ajog.2011.04.017

    authors： Rousset P,Chaillot PF,Bats AS,Le Frère Belda MA,Buy JN

    更新日期：2011-10-01 00:00:00

  • Estrogen and progesterone receptor and hormone levels in human myometrium and placenta in term pregnancy.

    abstract：:Estradiol and progesterone receptors in the myometrium, decidua, placenta, chorion, and amnion of eight women who underwent elective cesarean section at term were determined by means of an exchange assay. The hormone levels in the peripheral plasma and cytosol of these tissues were measured by radioimmunoassays. Mater...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 杂志文章

    doi：10.1016/s0002-9378(84)90428-9

    authors： Khan-Dawood FS,Dawood MY

    更新日期：1984-11-01 00:00:00

  • The cervical polyp: a new diagnostic and therapeutic approach with CO2 hysteroscopy.

    abstract：:CO2 hysteroscopy for diagnosis of cervical polyps was performed in 36 patients. The polyps could be located under direct vision and their attachments were found to be deeper than what is seen in regular inspection. A relatively high number of endometrial polyps were found. It is advised that the polyps be surgically r...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 杂志文章

    doi：10.1016/0002-9378(78)90324-1

    authors： David A,Mettler L,Semm K

    更新日期：1978-03-15 00:00:00

  • Associations between the vaginal microbiome and Candida colonization in women of reproductive age.

    abstract：BACKGROUND:The composition of bacteria within the vaginal microbiome has garnered a lot of recent attention and has been associated with reproductive health and disease. Despite the common occurrence of yeast (primarily Candida) within the vaginal microbiome, there is still an incomplete picture of relationships betwee...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 杂志文章

    doi：10.1016/j.ajog.2019.10.008

    authors： Tortelli BA,Lewis WG,Allsworth JE,Member-Meneh N,Foster LR,Reno HE,Peipert JF,Fay JC,Lewis AL

    更新日期：2020-05-01 00:00:00

  • Recent developments in immunocontraception.

    abstract：:The possibility of controlling fertility by antibodies inactivating key reproductive hormones has been amply demonstrated by active and passive immunization in primates. Four birth control vaccines directed against human chorionic gonadotropin are currently in early clinical trials. The nature of these vaccines and th...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 临床试验,杂志文章

    doi：10.1016/s0002-9378(87)80135-7

    authors： Talwar GP,Gaur A

    更新日期：1987-10-01 00:00:00

  • Incidence of endometrial hyperplasia in postmenopausal women taking conjugated estrogens (Premarin) with medroxyprogesterone acetate or conjugated estrogens alone. The Menopause Study Group.

    abstract：OBJECTIVE:We evaluated four oral combinations of conjugated estrogens (Premarin) and medroxyprogesterone acetate in preventing endometrial hyperplasia, which can occur with conjugated estrogens alone. STUDY DESIGN:This was a 1-year prospective, double-blind, randomized, multicenter study in 1724 postmenopausal women. ...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 临床试验,杂志文章,多中心研究,随机对照试验

    doi：10.1016/s0002-9378(94)70129-6

    authors： Woodruff JD,Pickar JH

    更新日期：1994-05-01 00:00:00

  • Cardiovascular disease: pathogenesis, epidemiology, and risk among users of oral contraceptives who smoke.

    abstract：:Smoking increases the risk of lung cancer and cardiovascular disease among persons of both sexes. The risk of cardiovascular disease is further increased among users of oral contraceptives who smoke, particularly those who are >/=35 years old or carry the coagulation factor V Leiden mutation. Other important cardiovas...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 杂志文章,评审

    doi：10.1016/s0002-9378(99)70695-2

    authors： Castelli WP

    更新日期：1999-06-01 00:00:00

  • Gestational diabetes alters human placental vascular responses to changes in oxygen tension.

    abstract：OBJECTIVE:Our objective was to study tone responses caused by hypoxia, reoxygenation, and hydrogen peroxide in human placental vessels from gestational diabetic and normal term pregnancies. STUDY DESIGN:Isolated placental arteries and veins from women with well-controlled gestational diabetic and uncomplicated term pr...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 杂志文章

    doi：10.1016/s0002-9378(11)90807-2

    authors： Figueroa R,Omar HA,Tejani N,Wolin MS

    更新日期：1993-05-01 00:00:00

  • The initiation, continuation, and termination of spontaneous episodes of circus movements in the pregnant myometrium of the rat.

    abstract：OBJECTIVES:The objectives of this study were to determine the mechanisms responsible for the initiation and the termination of episodes of circus movements and to describe the characteristics of these circuits in rat myometrium. STUDY DESIGN:By means of simultaneous recordings from 240 sites in Wistar rats at 17, 19, ...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 杂志文章

    doi：10.1016/s0002-9378(98)70018-3

    authors： Lammers WJ,Hamid R

    更新日期：1998-12-01 00:00:00

  • Male hormonal contraception.

    abstract：:Male hormonal contraception, based on the suppression of both gonadotropins, follicle-stimulating hormone and luteinizing hormone, resulting in marked decrease in sperm production, is designed for couples in stable relationships where the male partner desires to assume family planning responsibilities using reversible...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 杂志文章,评审

    doi：10.1016/j.ajog.2004.01.057

    authors： Wang C,Swerdloff RS

    更新日期：2004-04-01 00:00:00

  • Value of observation of fetal breathing activity in antenatal assessment of high-risk pregnancy.

    abstract：:While observation of fetal breathing movements has been used in fetal assessment, quantitative parameters (percent time spent in breathing [incidence], breath rate, or breath rate variability) have not been adequately evaluated as predictive tools. We examined 283 patients with high-risk pregnancies between 32 and 42 ...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 杂志文章

    doi：10.1016/0002-9378(89)90112-9

    authors： Devoe LD,Ruedrich DA,Searle NS

    更新日期：1989-01-01 00:00:00

  • Human fibroblasts produce "pregnancy-specific" beta-1 glycoprotein in vitro.

    abstract：:Normal and genetic variant strains of nonmalignant human fibroblasts produce in vitro a substance indistinguishable from the recently described "pregnancy-specific" beta-1 glycoprotein (PSbetaG, PAPP-C, SP1). Demonstration of this presumed placenta-specific material in a nonplacental site raises questions about its no...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 杂志文章

    doi：10.1016/0002-9378(79)90938-4

    authors： Rosen SW,Kaminska J,Calvert IS,Aaronson SA

    更新日期：1979-08-01 00:00:00

  • Cardiac function in fetuses of type I diabetic mothers.

    abstract：:Cardiac function was cross-sectionally studied by means of M-mode and Doppler echocardiography in 40 fetuses of mothers with well-controlled insulin-dependent diabetes at 20 to 38 weeks of gestation. These variables were measured: interventricular septal thickness, ratio between the peak velocities during early passiv...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 杂志文章

    doi：10.1016/0002-9378(91)90526-w

    authors： Rizzo G,Arduini D,Romanini C

    更新日期：1991-03-01 00:00:00

  • Maternal weight and pregnancy complications.

    abstract：:The effect of obesity alone was investigated among 16,858 women who were delivered of their infants within a 12-month period. Complete analysis was possible for 9667 patients who were divided into four weight categories and separated into two groups, with and without complications. Perinatal mortality, infant size, an...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 杂志文章

    doi：10.1016/s0002-9378(85)80029-6

    authors： Garbaciak JA Jr,Richter M,Miller S,Barton JJ

    更新日期：1985-05-15 00:00:00

  • Incidence of cervical precancers among HIV-seropositive women.

    abstract：OBJECTIVE:The objective of the study was to estimate the impact of human immunodeficiency virus (HIV) infection on the incidence of high-grade cervical intraepithelial neoplasia (CIN). STUDY DESIGN:HIV-seropositive and comparison seronegative women enrolled in a prospective US cohort study were followed up with semian...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 杂志文章

    doi：10.1016/j.ajog.2014.12.003

    authors： Massad LS,Xie X,D'Souza G,Darragh TM,Minkoff H,Wright R,Colie C,Sanchez-Keeland L,Strickler HD

    更新日期：2015-05-01 00:00:00

  • Long-term observations of cervical cancer.

    abstract：:A consecutive series of approximately 1100 patients, treated for cancer of the cervix over a span of 45 years, presents an increasingly favorable stage distribution. Emergence of exfoliative cytology as a diagnostic technique accelerates allocation of new patients to Stage I. Cumulative rates of dying from cervical ca...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 杂志文章

    doi：10.1016/0002-9378(87)90063-9

    authors： Arneson AN,Kao MS

    更新日期：1987-03-01 00:00:00

  • Cardiovascular collapse during laparoscopy: a report of two cases.

    abstract：:Cardiovascular collapse is a rare but serious complication of laparoscopy. Presented here are two cases of sudden cardiovascular collapse during laparoscopy. Possible causes include severe atypical vagal reactions and carbon dioxide embolism. Arterial blood gases indicated pulmonary vascular shunting. ...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 杂志文章

    doi：10.1016/s0002-9378(88)80044-9

    authors： Brantley JC 3rd,Riley PM

    更新日期：1988-09-01 00:00:00

  • Effects of estrogen on urethral function in women with urinary incontinence.

    abstract：:In a prospective study, 2 gm of conjugated estrogen vaginal cream was administered daily for a total of 6 weeks in a group of 11 postmenopausal women with urodynamically proved genuine stress incontinence. Midurethral cytologic studies and a complete clinical and urodynamic evaluation were performed twice at 6-week in...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 杂志文章

    doi：10.1016/0002-9378(89)90114-2

    authors： Bhatia NN,Bergman A,Karram MM

    更新日期：1989-01-01 00:00:00

  • The clinical content of preconception care: environmental exposures.

    abstract：:Environmental origins of disease risk and harm to health have been increasingly acknowledged for numerous outcomes, in both adult and pediatric populations. Adverse reproductive and developmental effects have also been linked to environmental exposures. In addition to the current queries about a patient's alcohol and ...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 杂志文章

    doi：10.1016/j.ajog.2008.10.044

    authors： McDiarmid MA,Gardiner PM,Jack BW

    更新日期：2008-12-01 00:00:00

  • Pregnancy increases cardiovascular toxicity to cocaine.

    abstract：:The effects of intravenous cocaine on heart rate and blood pressure were studied in pregnant and oophorectomized nonpregnant ewes. In response to intravenous cocaine doses of 1.0 and 2.0 mg/kg, both pregnant and nonpregnant ewes demonstrated dose-dependent increases in systolic, diastolic, mean arterial, and pulse pre...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 杂志文章

    doi：10.1016/0002-9378(90)90424-6

    authors： Woods JR Jr,Plessinger MA

    更新日期：1990-02-01 00:00:00

  • Effects of hormone replacement therapy on the mammary gland of surgically postmenopausal cynomolgus macaques.

    abstract：OBJECTIVE:Our purpose was to define the proliferative response and receptor status in the mammary glands of surgically postmenopausal macaques given hormone replacement therapy, equivalent for monkeys to that given women. STUDY DESIGN:Surgically postmenopausal adult female cynomolgus macaques (Macaca fascicularis) wer...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 杂志文章

    doi：10.1016/s0002-9378(96)70379-4

    authors： Cline JM,Soderqvist G,von Schoultz E,Skoog L,von Schoultz B

    更新日期：1996-01-01 00:00:00