Report and summary of the major conclusions from statistics in genotoxicity testing working group from the International Workshop on Genotoxicity Test Procedures (IWGTP), March 1999.

abstract：:The objective of our study was to develop a micronucleus (MN) assay for detecting genotoxic damage after inhalation exposure in mouse alveolar Type II and Clara cells, potential target cells for lung carcinogens. Ten male C57BL/6J mice were exposed to ethylene oxide (630 mg/m(3)) for 4 hr via inhalation; 10 unexposed ...

journal_title：Environmental and molecular mutagenesis

authors： Lindberg HK,Falck GC,Catalán J,Santonen T,Norppa H

更新日期：2010-03-01 00:00:00

abstract：:Occupational exposure to pesticides in tobacco fields causes genetic damage in farmers. The aim of this study was to analyze tobacco farmers chronically exposed to low doses of pesticides and nicotine (present in the tobacco leaves) in relation to absolute telomere length (aTL), and explore the influence of lifestyle ...

journal_title：Environmental and molecular mutagenesis

authors： Kahl VF,Simon D,Salvador M,Branco Cdos S,Dias JF,da Silva FR,de Souza CT,da Silva J

更新日期：2016-01-01 00:00:00

abstract：:Experimental features of a transgenic mouse mutation assay based on a lacI target transgene from Escherichia coli are considered in detail. Sources of variability in the experimental protocol that can affect the statistical nature of the observations are examined with the goal of identifying sources of excess variatio...

journal_title：Environmental and molecular mutagenesis

authors： Piegorsch WW,Lockhart AM,Margolin BH,Tindall KR,Gorelick NJ,Short JM,Carr GJ,Thompson ED,Shelby MD

更新日期：1994-01-01 00:00:00

abstract：:The accessibility of reactive metabolites to test cells is critical for a genotoxic response. However, sulfo-conjugates formed outside may not readily enter cells, and some metabolites formed by cytochromes P450 (CYPs) may not endure transport. This topic was addressed in the present study, using V79 cells engineered ...

journal_title：Environmental and molecular mutagenesis

authors： Zhu N,Hu K,Li Z,Chen Y,Liu Y

更新日期：2020-02-01 00:00:00

abstract：:Several types of diesel exhaust particles (DEPs) have been used for toxicology studies, including a high-organic automobile DEP (A-DEP) from Japan, and a low-organic forklift DEP developed by the National Institute of Standards and Technology (N-DEP). However, these DEPs were not characterized extensively for chemical...

journal_title：Environmental and molecular mutagenesis

authors： Mutlu E,Warren SH,Matthews PP,King C,Linak WP,Kooter IM,Schmid JE,Ross JA,Gilmour MI,Demarini DM

更新日期：2013-12-01 00:00:00

abstract：:The Pig-a assay is an emerging and promising in vivo method to determine mutagenic potential of chemicals. Since its development in 2008, remarkable progress has been made in harmonizing and characterizing the test procedures, primarily using known mutagenic chemicals. The purpose of the present study was to evaluate ...

journal_title：Environmental and molecular mutagenesis

authors： Ji Z,Settivari RS,LeBaron MJ

更新日期：2019-01-01 00:00:00

abstract：:Human sulfotransferase (SULT) 1A1, 1A2, and 1A3 cDNA genes were subcloned separately into the pTrc99A(KM) vector. The generated plasmids were introduced into the Salmonella typhimurium O-acetyltransferase-deficient strain NM6000 (TA1538/1,8-DNP/pSK1002), resulting in the new strains NM7001, NM7002, and NM7003. We comp...

journal_title：Environmental and molecular mutagenesis

authors： Oda Y,Zhang Y,Buchinger S,Reifferscheid G,Yang M

更新日期：2012-03-01 00:00:00

abstract：:The nuclease P1 modification of the 32P-postlabeling technique was used to study the biological activity of 7H-dibenzo[c,g]carbazole (DBC) and some of its derivatives, including N-methyldibenzo[c,g]carbazole (N-MeDBC), 5,9-dimethyldibenzo[c,g]carbazole (5,9-diMeDBC), 5,9,N-trimethyldibenzo[c,g]carbazole (5,9,N-triMeDB...

journal_title：Environmental and molecular mutagenesis

authors： Gábelová A,Périn-Roussel O,Jounaidi Y,Périn F

更新日期：1997-01-01 00:00:00

abstract：:Experiments described herein were designed to evaluate the performance characteristics of a flow cytometry-based system that scores the incidence of peripheral blood micronucleated reticulocytes (MN-RETs). These procedures represent the continued refinement of a previously reported anti-CD71-based method (Dertinger et...

journal_title：Environmental and molecular mutagenesis

authors： Dertinger SD,Camphausen K,Macgregor JT,Bishop ME,Torous DK,Avlasevich S,Cairns S,Tometsko CR,Menard C,Muanza T,Chen Y,Miller RK,Cederbrant K,Sandelin K,Pontén I,Bolcsfoldi G

更新日期：2004-01-01 00:00:00

abstract：:Polluted soil sampled from a former coking plant in Lorraine (France) was studied for its genotoxicity and reproductive effects on the Eisenia fetida earthworm. Genotoxicity was investigated by means of the single-cell gel electrophoresis (comet) assay on the coelomocytes of earthworms after 4 and 10 days of exposure ...

journal_title：Environmental and molecular mutagenesis

authors： Bonnard M,Eom IC,Morel JL,Vasseur P

更新日期：2009-01-01 00:00:00

abstract：:A study of meiotic and postmeiotic germ-cell-stage sensitivity of male mice to induction of unscheduled DNA synthesis (UDS) by acrylamide showed that DNA repair could be detected in early spermatocytes (after the last scheduled DNA synthesis) through about mid-spermatid stages. No DNA repair could be detected in later...

journal_title：Environmental and molecular mutagenesis

authors： Sega GA,Generoso EE,Brimer PA

更新日期：1990-01-01 00:00:00

abstract：:Diesel exhaust (DE) is a major airborne pollutant of urban areas. It contains various polycyclic aromatic hydrocarbons (PAH) and nitrated PAHs. In this study, gpt delta mice were treated with inhalation of 1 or 3 mg m(-3) DE, or a single intratracheal instillation of diesel exhaust particles (DEP) or DEP extract. In t...

journal_title：Environmental and molecular mutagenesis

authors： Hashimoto AH,Amanuma K,Hiyoshi K,Sugawara Y,Goto S,Yanagisawa R,Takano H,Masumura K,Nohmi T,Aoki Y

更新日期：2007-10-01 00:00:00

abstract：:Occupational exposure to wood dust is associated with the occurrence of nasal cancer. In this study, we investigated micronuclei and nuclear changes (NCs: binucleates, karyorrhexis, karyolysis, and the "broken egg" effect) in exfoliated buccal cells of 20 workers exposed to wood dust and 20 age- and sex-matched contro...

journal_title：Environmental and molecular mutagenesis

authors： Celik A,Kanik A

更新日期：2006-12-01 00:00:00

abstract：:To evaluate whether blood-based genotoxicity endpoints can provide temporal and dose-response data within the low-dose carcinogenic range that could contribute to carcinogenic mode of action (MoA) assessments, we evaluated the sensitivity of flow cytometry-based micronucleus and Pig-a gene mutation assays at and below...

journal_title：Environmental and molecular mutagenesis

authors： Dertinger SD,Phonethepswath S,Avlasevich SL,Torous DK,Mereness J,Cottom J,Bemis JC,Macgregor JT

更新日期：2014-05-01 00:00:00

abstract：BACKGROUND:Recently, high concentrations of arsenic have been documented in ground waters of Southern Assam, India. Indiscriminate smokeless tobacco consumption is a common practice in this region. Correlation between nutritional status and arsenic and smokeless tobacco-induced health effects has not been taken up in h...

journal_title：Environmental and molecular mutagenesis

authors： Das S,Langthasa P,Barhoi D,Upadhaya P,Giri S

更新日期：2018-06-01 00:00:00

abstract：:There is scientific and regulatory interest in using mutation assays in transgenic mice in safety assessments for new chemicals and drugs. Currently these assays are in the process of being validated, and protocols for routine testing are being defined. Some of the issues and results to date with regard to assay valid...

journal_title：Environmental and molecular mutagenesis

authors： Gorelick NJ

更新日期：1995-01-01 00:00:00

abstract：:Animal studies have linked perinatal bisphenol A (BPA) exposure to altered DNA methylation, but little attention is given to analyzing multiple physiologically relevant doses. Utilizing the viable yellow agouti (A(vy)) mouse, we examine the effects of developmental exposure through maternal diet to 50 ng BPA/kg (n = 1...

journal_title：Environmental and molecular mutagenesis

authors： Anderson OS,Nahar MS,Faulk C,Jones TR,Liao C,Kannan K,Weinhouse C,Rozek LS,Dolinoy DC

更新日期：2012-06-01 00:00:00

abstract：:Acute lymphoblastic leukemia (ALL) is the most common form of pediatric cancer. Although exposure to environmental agents appears to predispose individuals to this disease, little attention has been paid to the role of genetic susceptibility to environmental exposures in the etiology of childhood ALL. The enzymes GSTM...

journal_title：Environmental and molecular mutagenesis

authors： Canalle R,Burim RV,Tone LG,Takahashi CS

更新日期：2004-01-01 00:00:00

abstract：:Cytochrome P450 (CYP), glutathione-S-transferase (GST), and N-acetyltransferase (NAT) are crucial for metabolism and clearance of xenobiotics. This study investigated whether CYP, GST, and NAT single nucleotide polymorphisms (SNPs) are associated with colorectal cancer (CRC) in patients with Lynch syndrome. The intera...

journal_title：Environmental and molecular mutagenesis

authors： Kamiza AB,You JF,Wang WC,Tang R,Chang CY,Chien HT,Lai CH,Chiu LL,Lo TP,Hung KY,Hsiung CA,Yeh CC

更新日期：2018-01-01 00:00:00

abstract：:We review our studies on the role of polycyclic aromatic hydrocarbon (PAH)-DNA adducts in breast cancer. Additionally we report on analyses of the reliability of the scoring procedures used with immunohistochemical assay for PAH-DNA adducts and of potential bias arising from the use of benign breast disease (BBD) cont...

journal_title：Environmental and molecular mutagenesis

authors： Rundle A,Tang D,Hibshoosh H,Schnabel F,Kelly A,Levine R,Zhou J,Link B,Perera F

更新日期：2002-01-01 00:00:00

abstract：:In vitro transcriptional signatures that predict toxicities can facilitate chemical screening. We previously developed a transcriptomic biomarker (known as TGx-28.65) for classifying agents as genotoxic (DNA damaging) and non-genotoxic in human lymphoblastoid TK6 cells. Because TK6 cells do not express cytochrome P450...

journal_title：Environmental and molecular mutagenesis

authors： Yauk CL,Buick JK,Williams A,Swartz CD,Recio L,Li HH,Fornace AJ Jr,Thomson EM,Aubrecht J

更新日期：2016-05-01 00:00:00

abstract：:DNA damage induced by solar ultraviolet (UV) radiation plays an important role in the induction of skin cancer. Although UVA constitutes the majority of solar UV radiation, it is less damaging to DNA than UVB. The DNA damage produced by UVA radiation, however, can be augmented in the presence of a photosensitizer. We ...

journal_title：Environmental and molecular mutagenesis

authors： Toyooka T,Ibuki Y,Takabayashi F,Goto R

更新日期：2006-01-01 00:00:00

abstract：:Based on the assumption that compounds having similar toxic modes of action induce specific gene expression changes, the toxicity of unknown compounds can be predicted after comparison of their molecular fingerprints with those obtained with compounds of known toxicity. These predictive models will therefore rely on t...

journal_title：Environmental and molecular mutagenesis

authors： Thybaud V,Le Fevre AC,Boitier E

更新日期：2007-06-01 00:00:00

abstract：:In order to characterize in vivo gene mutations that occur during fetal development, molecular analyses were undertaken of mutant 6-thioguanine resistant T-lymphocytes isolated from placental cord blood samples of 13 normal male newborns. These mutant T-cells were studied to define hypoxanthine-guanine phosphoribosylt...

journal_title：Environmental and molecular mutagenesis

authors： McGinniss MJ,Nicklas JA,Albertini RJ

更新日期：1989-01-01 00:00:00

abstract：:The frequency of 6-thioguanine resistant (TGr) mutants induced in human G0 phase T-lymphocytes by 200 cGy of gamma irradiation is greatly enhanced by incubation with cytosine arabinoside (ara-C) after irradiation. The mutant frequency increased with increasing incubation time in ara-C for up to 2 hr. This mutation ind...

journal_title：Environmental and molecular mutagenesis

authors： O'Neill JP,Sullivan LM,Hunter TC,Nicklas JA

更新日期：1991-01-01 00:00:00

abstract：:Mutagenicity is an important toxicological endpoint that requires thorough evaluation during the industrial chemical registration process. Regulatory requirements for mutagenicity assessment in registration of industrial chemicals vary in geographic regions (and in some cases by intended application). Here we compile ...

journal_title：Environmental and molecular mutagenesis

authors： Ji Z,Ball NS,LeBaron MJ

更新日期：2017-06-01 00:00:00

abstract：:The aim of this research was to study the gaseous and particulate emissions of genotoxic substances during cooking with two types of methane stoves (a new one and an old one). The particulates were sampled both with a cascade impactor air sampler and an impinger with ice trap and analyzed by two bacterial mutagenicity...

journal_title：Environmental and molecular mutagenesis

authors： Monarca S,Zanardini A,Feretti D,Falistocco E,Antonelli P,Resola S,Moretti M,Villarini M,Nardi G

更新日期：1998-01-01 00:00:00

abstract：:Prostate cancer (PC) is commonly diagnosed cancer in men but only a few risk factors, such as family history, ethnicity, and age have been established. Chromosomal instability is another possible risk factor but this has not been adequately explained previously. In this study, we tested the hypotheses that peripheral ...

journal_title：Environmental and molecular mutagenesis

authors： Dhillon VS,Yeoh E,Salisbury C,Butters J,Di Matteo A,Olver I,Fenech M

更新日期：2018-12-01 00:00:00

abstract：:Several genotoxicity endpoints have been evaluated to define nonlinear dose-responses for SN 1 and SN 2 alkylating genotoxicants. Dose-response studies acknowledging the process of multistage tumorigenesis are important; however, data pertaining nonlinearity are not yet available. In this communication, the role of DN...

journal_title：Environmental and molecular mutagenesis

authors： Becker K,Thomas AD,Kaina B

更新日期：2014-03-01 00:00:00

abstract：:The genotoxic responses of mixtures of four chlorohydroxyfuranones (CHFs), 3-chloro-4-(dichloromethyl)-5-hydroxy-2(5H)-furanone (MX), 3,4-dichloro-5-hydroxy-2(5H)-furanone (MCA), 3-chloro- 4-(chloromethyl)-5-hydroxy-2(5H)-furanone (CMCF) and 3-chloro-4-methyl-5-hydroxy-2(5H)-furanone (MCF), were compared with the geno...

journal_title：Environmental and molecular mutagenesis

authors： Mäki-Paakkanen J,Komulainen H,Kronberg L

更新日期：2004-01-01 00:00:00