Abstract：
:Current research in gene delivery to the liver is focused on the intravenous, intraarterial, intraportal, or intratumoral route. Another possible route for gene delivery is via the common bile duct through endoscopic retrograde cholangiopancreatography (ERCP). Whether bile and pancreatic juice have any effect on gene delivery is not established. To evaluate the effect of bile and pancreatic juice on adenoviral-mediated gene delivery, liver and pancreatic cell lines were infected with a recombinant adenovirus expressing an E. coli beta-galactosidase gene under the control of a cytomegalovirus promoter (rAdCMVpLacZ) in the absence or presence of various concentrations of bile and pancreatic juice. The proportion of cells infected was evaluated through X-gal staining. The toxicity of bile and pancreatic juice was also evaluated through cell morphology and detachment. Bile appeared to induce significant cytotoxicity in HepG2 and Huh7 cells (50% viability with 15 min of incubation). Neither bile nor pancreatic juice affected transgene expression. In the absence of bile/pancreatic juice, HepG2 (15-25%) and PANC-1 cells (10-18%) were less susceptible to rAdCMVpLacZ compared to Huh7 cells (75-84%, vs HepG2, P < 0.001) and BxPc-3 (82-95%, vs PANC-1, P < 0.001) at a multiplicity of infection (MOI) of 5. Bile reduced the transduction efficiency, but 5-10% HepG2 and 5-42% of Huh7 cells were still transduced in the presence of 80% bile for up to 10 min. Adenoviral-mediated gene delivery was reduced in the presence of pancreatic juice with a low multiplicity of infection (MOI of 5), but this effect was negated with an MOI of 50. These data provide encouragement to develop adenoviral-mediated gene delivery through ERCP.
journal_name
Dig Dis Scijournal_title
Digestive diseases and sciencesauthors
Xie X,Forsmark CE,Lau JYdoi
10.1023/a:1005431703317subject
Has Abstractpub_date
2000-02-01 00:00:00pages
230-6issue
2eissn
0163-2116issn
1573-2568journal_volume
45pub_type
杂志文章abstract：BACKGROUND:Colorectal cancer (CRC) screening is a priority for the Veteran's Health Administration (VHA). Optimizing fecal occult blood testing (FOBT) is integral to CRC screening in health care systems. AIMS:The purpose of this study was to characterize the utilization of CRC testing in a large integrated health care...
journal_title：Digestive diseases and sciences
pub_type： 杂志文章
doi：10.1007/s10620-011-1895-4
更新日期：2012-02-01 00:00:00
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journal_title：Digestive diseases and sciences
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journal_title：Digestive diseases and sciences
pub_type： 杂志文章
doi：10.1007/BF01297466
更新日期：1991-08-01 00:00:00
abstract：:The motor function of the gallbladder after partial distal gastrectomy for gastric carcinoma (Billroth-I reconstruction) and its relationship to the motor function of the stomach was investigated using a double-isotope method. In this method, [99mTc]N-54-pyridoxyl-5-methyltryptophan ([99mTc]PMT) was utilized as a trac...
journal_title：Digestive diseases and sciences
pub_type： 杂志文章
doi：10.1007/BF02064968
更新日期：1995-04-01 00:00:00
abstract：:A series of in vitro experiments was performed to test the ability of bovine and human lactoferrin to influence the growth of the gram-positive probiotic bacteria, Bifidobacterium bifidum, Bifidobacterium infantis, and Lactobacillus acidophilus, as well as the gram-negative enteric bacteria, E. coli O157:H7 and Salmon...
journal_title：Digestive diseases and sciences
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journal_title：Digestive diseases and sciences
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journal_title：Digestive diseases and sciences
pub_type： 杂志文章
doi：10.1007/s10620-009-0715-6
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journal_title：Digestive diseases and sciences
pub_type： 杂志文章
doi：10.1007/s10620-007-0074-0
更新日期：2008-07-01 00:00:00
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journal_title：Digestive diseases and sciences
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doi：10.1007/s10620-009-0964-4
更新日期：2010-07-01 00:00:00
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journal_title：Digestive diseases and sciences
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更新日期：2020-08-01 00:00:00
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journal_title：Digestive diseases and sciences
pub_type： 杂志文章
doi：10.1007/BF01308045
更新日期：1980-12-01 00:00:00
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journal_title：Digestive diseases and sciences
pub_type： 杂志文章
doi：10.1007/s10620-012-2399-6
更新日期：2013-03-01 00:00:00
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journal_title：Digestive diseases and sciences
pub_type： 杂志文章
doi：10.1007/BF01537413
更新日期：1990-03-01 00:00:00
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journal_title：Digestive diseases and sciences
pub_type： 杂志文章,评审
doi：10.1007/s10620-015-3907-2
更新日期：2016-02-01 00:00:00
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journal_title：Digestive diseases and sciences
pub_type： 杂志文章
doi：10.1023/a:1010644021888
更新日期：2001-07-01 00:00:00
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journal_title：Digestive diseases and sciences
pub_type： 杂志文章
doi：10.1023/a:1024799303676
更新日期：2003-08-01 00:00:00
abstract：:We have developed a breath test to measure solid gastric emptying using a standardized scrambled egg test meal (250 kcal) labeled with [14C]octanoic acid or [13C]octanoic acid. In vitro incubation studies showed that octanoic acid is a reliable marker of the solid phase. The breath test was validated in 36 subjects by...
journal_title：Digestive diseases and sciences
pub_type： 杂志文章
doi：10.1007/BF02300385
更新日期：1994-12-01 00:00:00
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journal_title：Digestive diseases and sciences
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doi：10.1007/s10620-020-06517-7
更新日期：2020-08-04 00:00:00
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journal_title：Digestive diseases and sciences
pub_type： 杂志文章,评审
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更新日期：1986-02-01 00:00:00
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journal_title：Digestive diseases and sciences
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更新日期：2000-01-01 00:00:00
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journal_title：Digestive diseases and sciences
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更新日期：2009-04-01 00:00:00
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journal_title：Digestive diseases and sciences
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doi：10.1007/BF01537263
更新日期：1989-08-01 00:00:00
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journal_title：Digestive diseases and sciences
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doi：10.1007/BF01308293
更新日期：1985-10-01 00:00:00
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更新日期：2009-08-01 00:00:00
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更新日期：2007-04-01 00:00:00
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journal_title：Digestive diseases and sciences
pub_type： 杂志文章
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更新日期：2012-04-01 00:00:00
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journal_title：Digestive diseases and sciences
pub_type： 杂志文章,meta分析
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更新日期：2008-12-01 00:00:00
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更新日期：2012-11-01 00:00:00
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journal_title：Digestive diseases and sciences
pub_type： 杂志文章
doi：10.1007/BF02209017
更新日期：1995-10-01 00:00:00
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journal_title：Digestive diseases and sciences
pub_type： 杂志文章
doi：10.1007/s10620-011-1896-3
更新日期：2012-02-01 00:00:00
