Abstract:
:The tumor suppressor gene p53 is a potent transcriptional regulator of genes which are involved in many cellular activities including cell cycle arrest, apoptosis, and angiogenesis. Recent studies have demonstrated that the activation of the transcriptional factor nuclear factor kappaB (NF-kappaB) plays an essential role in preventing apoptotic cell death. In this study, to better understand the mechanism responsible for the p53-mediated apoptosis, the effect of wild-type p53 (wt-p53) gene transfer on nuclear expression of NF-kappaB was determined in human colon cancer cell lines. A Western blot analysis of nuclear extracts demonstrated that NF-kappaB protein levels in the nuclei were suppressed by the transient expression of the wt-p53 in a dose-dependent manner. Transduced wt-p53 expression increased the cytoplasmic expression of I kappaB alpha as well as its binding ability to NF-kappaB, thus markedly reducing the amount of NF-kappaB that translocated to the nucleus. The decrease in nuclear NF-kappaB protein correlated with the decreased NF-kappaB constitutive activity measured by electrophoretic mobility shift assay. Furthermore, parental cells transfected with NF-kappaB were better protected from cell death induced by the wt-p53 gene transfer. We also found that the wt-p53 gene transfer was synergistic with aspirin (acetylsalicylic acid) in inhibiting NF-kappaB constitutive activity, resulting in enhanced apoptotic cell death. These results suggest that the inhibition of NF-kappaB activity is a plausible mechanism for apoptosis induced by the wt-p53 gene transfer in human colon cancer cells and that anti-NF-kappaB reagent aspirin could make these cells more susceptible to apoptosis.
journal_name
Oncogenejournal_title
Oncogeneauthors
Shao J,Fujiwara T,Kadowaki Y,Fukazawa T,Waku T,Itoshima T,Yamatsuji T,Nishizaki M,Roth JA,Tanaka Ndoi
10.1038/sj.onc.1203383subject
Has Abstractpub_date
2000-02-10 00:00:00pages
726-36issue
6eissn
0950-9232issn
1476-5594journal_volume
19pub_type
杂志文章相关文献
ONCOGENE文献大全abstract::Damage-associated molecular patterns (DAMPs) are released in response to cell death and stress, and are potent triggers of sterile inflammation. Recent evidence suggests that DAMPs may also have a key role in the development of cancer, as well as in the host response to cytotoxic anti-tumor therapy. As such, DAMPs may...
journal_title:Oncogene
pub_type: 杂志文章,评审
doi:10.1038/onc.2016.104
更新日期:2016-11-17 00:00:00
abstract::Although cell invasion is a necessary early step in cancer metastasis, its regulation is not well understood. We have previously shown, in human prostate cancer, that transforming growth factor beta (TGFbeta)-mediated increases in cell invasion are dependent upon activation of the serine/threonine kinase, p38 MAP kina...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1209337
更新日期:2006-05-18 00:00:00
abstract::Expression microarray analysis identified CITED1 among a group of genes specifically upregulated in the pubertal mouse mammary gland. At puberty, CITED1 localizes to the luminal epithelial cell population of the mammary ducts and the body cells of the terminal end buds. Generation of CITED1 gene knockout mice showed t...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1209183
更新日期:2006-03-09 00:00:00
abstract::Nuclear factor-kappaB (NF-kappaB) is a dynamic transcription factor that regulates important biological processes involved in cancer initiation and progression. Identifying regulators that control the half-life of NF-kappaB is important to understanding molecular processes that control the duration of transcriptional ...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1211030
更新日期:2008-06-05 00:00:00
abstract::Melanoma progression is typically depicted as a linear and stepwise process in which metastasis occurs relatively late in disease progression. Significant evidence suggests that in a subset of melanomas, progression is much more complex and less linear in nature. Epidemiologic and experimental observations in melanoma...
journal_title:Oncogene
pub_type: 杂志文章,评审
doi:10.1038/onc.2013.194
更新日期:2014-05-08 00:00:00
abstract::Targeting Bruton tyrosine kinase (BTK) by ibrutinib is an effective treatment for patients with relapsed/refractory mantle cell lymphoma (MCL). However, both primary and acquired resistance to ibrutinib have developed in a significant number of these patients. A combinatory strategy targeting multiple oncogenic pathwa...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2016.155
更新日期:2016-12-01 00:00:00
abstract::Tumors of low malignant potential (LMP) represent 20% of epithelial ovarian cancers (EOCs) and are associated with a better prognosis than the invasive tumors (TOV). Defining the relationship between LMPs and TOVs remains an important goal towards understanding the molecular pathways that contribute to prognosis, as w...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1208214
更新日期:2005-07-07 00:00:00
abstract::A hallmark of plasma cells is the expression of syndecan-1, which has major functions in epithelial cells, in particular as the coreceptor of heparin-binding growth factors. We previously found that heparin-binding epidermal growth factor-like growth factor (HB-EGF) is a growth factor for malignant plasma cells. As am...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1208536
更新日期:2005-05-12 00:00:00
abstract::Small cell lung cancer (SCLC) invades locally and metastasizes distantly extremely early when compared with nonsmall cell lung cancer (NSCLC). The underlying molecular mechanisms, however, have not been elucidated. Accumulating evidence suggests that downregulation of several members of tetraspanins is associated with...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1206106
更新日期:2003-02-06 00:00:00
abstract::Activation of peroxisome proliferator-activated receptor (PPAR)-gamma by the thiazolidinedione (TZD) class of antidiabetic drugs elicits growth inhibition in a variety of malignant tumors. We clarified the effects of TZDs on growth of human non-small cell lung carcinoma (NSCLC) cells that express endogenous PPAR-gamma...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1205279
更新日期:2002-03-28 00:00:00
abstract::Polyamines are critical elements in mammals, but it remains unknown whether adenosyl methionine decarboxylase (AMD1), a rate-limiting enzyme in polyamine synthesis, is required for myeloid leukemia. Here, we found that leukemic stem cells (LSCs) were highly differentiated, and leukemia progression was severely impaire...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/s41388-020-01547-x
更新日期:2021-01-01 00:00:00
abstract::A flow cytometric assay was developed to examine the expression of the cellular myc oncogene in relation to cell cycle in individual cells. C-myc-oncoprotein was detected by indirect immunofluorescence using a purified sheep polyclonal antibody, anti-human-myc. Specific binding of anti-human-myc was measured by flow c...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1988-10-01 00:00:00
abstract::Oncogene-induced senescence (OIS) is an intrinsic tumor suppression mechanism that requires the p53 and RB pathways and post-translational activation of C/EBPβ through the RAS-ERK cascade. We previously reported that in transformed/proliferating cells, C/EBPβ activation is inhibited by G/U-rich elements (GREs) in its ...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/s41388-018-0190-7
更新日期:2018-06-01 00:00:00
abstract::D-type cyclins are important cell cycle regulators that promote cellular proliferation in response to growth factors by inactivation of the retinoblastoma protein (Rb). Cyclin D1 has been shown to be overexpressed in several cancer types and to act as an oncogene in breast cancers. As D-type cyclins are rate limiting ...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1202511
更新日期:1999-03-18 00:00:00
abstract::Proliferating cell nuclear antigen (PCNA) plays an essential role in nucleic acid metabolism as a component of the replication and repair machinery. This toroidal-shaped protein encircles DNA and can slide bidirectionally along the duplex. One of the well-established functions for PCNA is its role as the processivity ...
journal_title:Oncogene
pub_type: 杂志文章,评审
doi:10.1038/sj.onc.1200886
更新日期:1997-02-13 00:00:00
abstract::Adhesion to the extracellular matrix (ECM) is critical for epithelial tissue homeostasis and function. ECM detachment induces metabolic stress and programmed cell death via anoikis. ECM-detached mammary epithelial cells are able to rapidly activate autophagy allowing for survival and an opportunity for re-attachment. ...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2012.512
更新日期:2013-10-10 00:00:00
abstract::Oncogenic forms of the c-myb protein (Myb) often exhibit amino-terminal and/or carboxyl-terminal truncations. When the transcriptional activity of these proteins was examined it was found that carboxyl-truncated Myb is more effective as a transcriptional activator than full-length or amino-truncated Myb. In order to d...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1991-10-01 00:00:00
abstract::The retroviral oncogene v-myb encodes a transcription factor (v-Myb) which is responsible for the ability of avian myeloblastosis virus (AMV) to transform myelomonocytic cells. v-Myb is thought to disrupt the differentiation of myelomonocytic cells by affecting the expression of specific target genes. To identify such...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1204071
更新日期:2001-01-11 00:00:00
abstract::The majority (75%) of human breast cancers express estrogen receptor (ER). Although ER-positive tumors usually respond to antiestrogen therapies, 30% of them do not. It is not known what controls the ER status of breast cancers or their responsiveness to antihormone interventions. In this report, we document that tran...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1208597
更新日期:2005-06-16 00:00:00
abstract::Protein phosphatase inhibitors are often considered as tumor promoters. Protein phosphatase 1 regulatory subunit 1A (PPP1R1A) is a potent protein phosphatase 1 (PP1) inhibitor; however, its role in tumor development is largely undefined. Here we characterize, for the first time, the functions of PPP1R1A in Ewing sarco...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2017.378
更新日期:2018-02-08 00:00:00
abstract::Cancer cells acquire several traits that allow for their survival and progression, including the ability to evade the host immune response. However, the mechanisms by which cancer cells evade host immune responses remain largely elusive. Here we study the phenomena of immune evasion in malignant melanoma cells. We fin...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2013.409
更新日期:2014-09-18 00:00:00
abstract::Transfection of primate tissue explants with a specific sub-fragment (p31) of EBV DNA results in epithelial (but no other) cells proliferating indefinitely (becoming 'immortalized') without evidence of a 'growth crisis'. Molecular evidence supports integration of viral information into the host chromosome, and an earl...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1205130
更新日期:2002-01-24 00:00:00
abstract::The NAD-dependent deacetylase sirtuin 1 (SIRT1), a member of the mammalian sirtuin family, plays a pivotal role in deacetylating histone and nonhistone proteins. Recently, it has been reported that SIRT1 is upregulated in various kinds of tumors and is associated with cell growth and metastasis. However, the factors a...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/s41388-020-1298-0
更新日期:2020-05-01 00:00:00
abstract::BRCA1, a familial breast and ovarian cancer susceptibility gene encodes nuclear phosphoproteins that function as tumor suppressors in human breast cancer cells. Previously, we have shown that overexpression of a BRCA1 splice variant BRCA1a accelerates apoptosis in human breast cancer cells. In an attempt to determine ...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1201252
更新日期:1997-07-10 00:00:00
abstract::Metabolic acid production challenges cellular pH homeostasis in solid cancer tissue, and mechanisms of net acid extrusion represent promising new targets for breast cancer therapy. Here, we used genetically engineered mice to investigate the contribution of the Na+,HCO3--cotransporter NBCn1 (Slc4a7) to intracellular a...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/s41388-018-0353-6
更新日期:2018-10-01 00:00:00
abstract::Genetic analysis of beta-catenin-induced oncogenic transformation in chicken embryo fibroblasts (CEF) revealed the following prerequisites for oncogenicity: (1) removal of the N terminal phosphorylation sites targeted by glycogen synthase kinase 3beta (GSK3beta), (2) retention of the N terminal transactivation domain,...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1205796
更新日期:2002-10-10 00:00:00
abstract::We studied the role of the mitogen-activated protein kinase (MAPK) pathway in the regulation of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)-mediated apoptosis in breast tumor MCF-7 cells. We found that addition of a protein kinase C (PKC) activator to MCF-7 cultures prevented TRAIL-induced apoptosi...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1205523
更新日期:2002-06-20 00:00:00
abstract::We show that SV40 infection of human mesothelial cells directly causes overexpression of Notch-1, a key cell regulatory gene. Notch-1 induction is achieved at the transcriptional level and requires both the SV40 large T-antigen and the small t-antigen. Notch-1 upregulation is maintained in SV40-transformed human mesot...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1206097
更新日期:2003-01-09 00:00:00
abstract::Cyclooxygenase-2 (COX-2), the rate-limiting enzyme in the synthesis of prostaglandins, promotes the development of colorectal cancer, and is a key molecular target of non-steroidal anti-inflammatory drugs, compounds that reduce the relative risk of developing colon cancer. In this study, we showed that interferon gamm...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1210015
更新日期:2007-03-29 00:00:00
abstract::The trans-activator protein, tax, from the human T leukemia virus type 1 (HTLV-1) trans-activates both viral and cellular genes. It has previously been shown that granulocyte macrophage-colony stimulating factor (GM-CSF) is constitutively expressed in HTLV-1 infected cells and in cells artificially expressing tax. We ...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1993-12-01 00:00:00