当前位置： SCI文献检索 > LEUKEMIA期刊下所有文献 > Rapid and reliable detection of N-ras mutations in acute lymphoblastic leukemia by melting curve analysis using LightCycler technology.

Rapid and reliable detection of N-ras mutations in acute lymphoblastic leukemia by melting curve analysis using LightCycler technology.

Abstract：

:We applied a new strategy for the detection of N-ras gene mutations based on LightCycler technology. We designed two sets of amplimers and internal hybridization probes representing N-ras codons 12/13 and codon 61, respectively. Genomic DNAs from 134 childhood acute lymphoblastic leukemia (ALL) patients (83 common ALL, nine pre-pre-B ALL, 19 pre-B ALL, 23 T-ALL) were amplified, followed by the analysis of the melting temperatures of the PCR products on the LightCycler. PCR products exhibiting an abnormal melting characteristic were directly sequenced. Sequence analyses unravelled nucleotide substitutions at codon 12 in 10 patients, at codon 13 in three, and at codon 61 in one case. The incidence of N-rasmutations (10%) is compatible with previous reports. The LightCycler technology facilitates the rapid analysis of other genes exhibiting hot spot mutations in human malignancies.

journal_name

Leukemia

journal_title

Leukemia

authors

Nakao M,Janssen JW,Seriu T,Bartram CR

doi

10.1038/sj.leu.2401645

subject

Has Abstract

pub_date

2000-02-01 00:00:00

pages

312-5

issue

2

eissn

0887-6924

issn

1476-5551

journal_volume

14

pub_type

杂志文章

相关文献

LEUKEMIA文献大全
  • Incidence of extramedullary disease in patients with multiple myeloma in the era of novel therapy, and the activity of pomalidomide on extramedullary myeloma.

    abstract：:We studied 174 consecutive patients with relapsed refractory multiple myeloma (MM) enrolled on a phase II clinical trial of pomalidomide plus low-dose dexamethasone at Mayo Clinic. Extramedullary disease (EMD) was present at the time of trial entry in 7.5% (13 of 174 patients). The rate of EMD in the first 3 years fol...

    journal_title：Leukemia

    pub_type： 杂志文章

    doi：10.1038/leu.2011.29

    authors： Short KD,Rajkumar SV,Larson D,Buadi F,Hayman S,Dispenzieri A,Gertz M,Kumar S,Mikhael J,Roy V,Kyle RA,Lacy MQ

    更新日期：2011-06-01 00:00:00

  • A model system for leukemic transformation of immortalized hemopoietic cells in irradiated recipient mice.

    abstract：:Proliferation in vitro of the murine hemopoietic cell line FDC-P1 is dependent on stimulation by granulocyte-macrophage colony stimulating factor or multipotential colony stimulating factor. Although immortalized, the cells are not tumorigenic on subcutaneous inoculation. Intravenous injection of FDC-P1 cells into syn...

    journal_title：Leukemia

    pub_type： 杂志文章

    doi：

    authors： Dührsen U,Metcalf D

    更新日期：1988-06-01 00:00:00

  • Allelotype analysis of the myelodysplastic syndrome.

    abstract：:Myelodysplastic syndromes (MDS) are a group of clonal hematologic disorders found predominantly in the elderly. The molecular mechanisms underlying the development of MDS remain obscure. In order to begin to identify tumor suppressor genes involved in these disorders, we performed a detailed microsatellite allelotype ...

    journal_title：Leukemia

    pub_type： 杂志文章

    doi：10.1038/sj.leu.2401717

    authors： Xie D,Hofmann WK,Mori N,Miller CW,Hoelzer D,Koeffler HP

    更新日期：2000-05-01 00:00:00

  • Localization of the chromosome 15 breakpoints and expression of multiple PML-RAR alpha transcripts in acute promyelocytic leukemia: a study of 28 Chinese patients.

    abstract：:Translocation (15;17)(q22;q12-q21) is a chromosome aberration specifically found in acute promyelocytic leukemia (APL), that generates a chimeric gene between the promyelocytic leukemia (PML) gene on chromosome 15 and the retinoic acid receptor alpha (RARA) gene, on chromosome 17. In the course of molecular investigat...

    journal_title：Leukemia

    pub_type： 杂志文章

    doi：

    authors： Geng JP,Tong JH,Dong S,Wang ZY,Chen SJ,Chen Z,Zelent A,Berger R,Larsen CJ

    更新日期：1993-01-01 00:00:00

  • Identification of a leukemia-initiating stem cell in human mast cell leukemia.

    abstract：:Mast cell leukemia (MCL) is a highly fatal malignancy characterized by devastating expansion of immature mast cells in various organs. Although considered a stem cell disease, little is known about MCL-propagating neoplastic stem cells. We here describe that leukemic stem cells (LSCs) in MCL reside within a CD34+/CD38...

    journal_title：Leukemia

    pub_type： 杂志文章

    doi：10.1038/s41375-019-0460-6

    authors： Eisenwort G,Sadovnik I,Schwaab J,Jawhar M,Keller A,Stefanzl G,Berger D,Blatt K,Hoermann G,Bilban M,Willmann M,Winding C,Sperr WR,Arock M,Rülicke T,Reiter A,Valent P

    更新日期：2019-11-01 00:00:00

  • Perillyl alcohol selectively induces G0/G1 arrest and apoptosis in Bcr/Abl-transformed myeloid cell lines.

    abstract：:The Bcr/Abl tyrosine kinase that is expressed from the Philadelphia chromosome protects leukemia cells from apoptosis caused by removal of growth factors or by cytotoxic agents and ionizing irradiation. This resistance to apoptosis is associated with a Bcr/Abl-mediated G2/M delay. Therefore, inhibiting Bcr/Abl signali...

    journal_title：Leukemia

    pub_type： 杂志文章

    doi：10.1038/sj.leu.2401536

    authors： Sahin MB,Perman SM,Jenkins G,Clark SS

    更新日期：1999-10-01 00:00:00

  • TEL-Syk fusion constitutively activates PI3-K/Akt, MAPK and JAK2-independent STAT5 signal pathways.

    abstract：:We previously reported the fusion of the TEL gene to the Syk gene in myelodysplastic syndrome with t(9;12)(q22;p12). TEL-Syk fusion transformed interleukin-3 (IL-3)-dependent murine hematopoietic cell line BaF3 to growth factor independence. Here, we investigate the intracellular signal transduction of the stable tran...

    journal_title：Leukemia

    pub_type： 杂志文章

    doi：10.1038/sj.leu.2403266

    authors： Kanie T,Abe A,Matsuda T,Kuno Y,Towatari M,Yamamoto T,Saito H,Emi N,Naoe T

    更新日期：2004-03-01 00:00:00

  • Alpha interferon gene deletions in adults, children and infants with acute lymphoblastic leukemia.

    abstract：:DNA from 76 cases of acute lymphoblastic leukemia (ALL) was tested with a cDNA probe encoding the alpha 2B interferon (IFN) gene transcript. Deletions were found in three of ten pre-B, three of 21 T-cell, four of 22 common and one of 23 null ALL cases. Amongst those with null ALL were 20 infants, most with characteris...

    journal_title：Leukemia

    pub_type： 杂志文章

    doi：

    authors： Middleton PG,Prince RA,Williamson IK,Taylor PR,Reid MM,Jackson GH,Katz F,Chessells JM,Proctor SJ

    更新日期：1991-08-01 00:00:00

  • Cytogenetics and oncogenes.

    abstract：:There is abundant evidence that leukemia arise through somatically acquired genetic changes. Familial or congenital predisposition is rarely involved. These genetic changes are often visible as chromosomal aberrations. Molecular analyses of the DNA sequences rearranged in leukemia has demonstrated the presence of cell...

    journal_title：Leukemia

    pub_type： 杂志文章,评审

    doi：

    authors： Hagemeijer A

    更新日期：1992-11-01 00:00:00

  • Childhood monosomy 7 syndrome: clinical and in vitro studies.

    abstract：:The clinical and cell growth characteristics of 11 children with monosomy 7 presenting as preleukemia (eight cases) or acute nonlymphoblastic leukemia (three cases) were studied. Anemia was common to all patients, with nine showing leukocytosis, seven thrombocytopenia, and one thrombocytosis. There was a striking pred...

    journal_title：Leukemia

    pub_type： 杂志文章

    doi：

    authors： Weiss K,Stass S,Williams D,Kalwinsky D,Dahl GV,Wang W,Johnson FL,Murphy SB,Dow LW

    更新日期：1987-02-01 00:00:00

  • Comparative analysis of T-cell receptor gene rearrangements at diagnosis and relapse of T-cell acute lymphoblastic leukemia (T-ALL) shows high stability of clonal markers for monitoring of minimal residual disease and reveals the occurrence of second T-AL

    abstract：:A total of 28 children and nine adults with relapsed T-ALL were analyzed for the configuration of their T-cell receptor (TCR) and TAL1 genes at diagnosis and relapse to evaluate their stability throughout the disease course. A total of 150 clonal TCR and TAL1 gene rearrangements were identified in the 37 patients at d...

    journal_title：Leukemia

    pub_type： 杂志文章

    doi：10.1038/sj.leu.2403081

    authors： Szczepański T,van der Velden VH,Raff T,Jacobs DC,van Wering ER,Brüggemann M,Kneba M,van Dongen JJ

    更新日期：2003-11-01 00:00:00

  • In vivo sequence changes of HIV-1 envelope V2 and V3 loops.

    abstract：:Human immunodeficiency virus type 1 (HIV-1) circulates in vivo as heterogeneous mixed populations (quasispecies). We analyzed the quasispecies nature of the first, second, and third hypervariable loops (V1, V2, and V3) of the gp 120 in plasma and viral isolates obtained from 19 infected individuals. Sequence analysis ...

    journal_title：Leukemia

    pub_type： 杂志文章

    doi：

    authors： Shioda T,Oka S,Nagai Y

    更新日期：1997-04-01 00:00:00

  • Classification and diagnosis of myeloproliferative neoplasms: the 2008 World Health Organization criteria and point-of-care diagnostic algorithms.

    abstract：:The 2001 World Health Organization (WHO) treatise on the classification of hematopoietic tumors lists chronic myeloproliferative diseases (CMPDs) as a subdivision of myeloid neoplasms that includes the four classic myeloproliferative disorders (MPDs)-chronic myelogenous leukemia, polycythemia vera (PV), essential thro...

    journal_title：Leukemia

    pub_type： 杂志文章,评审

    doi：10.1038/sj.leu.2404955

    authors： Tefferi A,Vardiman JW

    更新日期：2008-01-01 00:00:00

  • The regulation of hematopoiesis in max 41 transgenic mice with sustained excess granulopoiesis.

    abstract：:max 41 transgenic mice consistently exhibit elevated numbers of mature granulocytes and monocytes in the peripheral blood and of immature and mature cells of these lineages in the marrow, spleen, lymph nodes and liver. The immature populations are not autonomous and exhibit a normal quantitative responsiveness to prol...

    journal_title：Leukemia

    pub_type： 杂志文章

    doi：

    authors： Metcalf D,Roberts AW,Willson TA

    更新日期：1996-02-01 00:00:00

  • Induction of microRNAs, mir-155, mir-222, mir-424 and mir-503, promotes monocytic differentiation through combinatorial regulation.

    abstract：:Acute myeloid leukemia (AML) involves a block in terminal differentiation of the myeloid lineage and uncontrolled proliferation of a progenitor state. Using phorbol myristate acetate (PMA), it is possible to overcome this block in THP-1 cells (an M5-AML containing the MLL-MLLT3 fusion), resulting in differentiation to...

    journal_title：Leukemia

    pub_type： 杂志文章

    doi：10.1038/leu.2009.246

    authors： Forrest AR,Kanamori-Katayama M,Tomaru Y,Lassmann T,Ninomiya N,Takahashi Y,de Hoon MJ,Kubosaki A,Kaiho A,Suzuki M,Yasuda J,Kawai J,Hayashizaki Y,Hume DA,Suzuki H

    更新日期：2010-02-01 00:00:00

  • Differential expression of NF-kappaB target genes in MALT lymphoma with and without chromosome translocation: insights into molecular mechanism.

    abstract：:Mucosa-associated lymphoid tissue (MALT) lymphoma is characterized by t(11;18)(q21;q21)/API2-MALT1, t(1;14)(p22;q32)/BCL10-IGH and t(14;18)(q32;q21)/IGH-MALT1, which commonly activate the nuclear factor (NF)-kappaB pathway. Gastric MALT lymphomas harboring such translocations usually do not respond to Helicobacter pyl...

    journal_title：Leukemia

    pub_type： 杂志文章

    doi：10.1038/leu.2010.118

    authors： Hamoudi RA,Appert A,Ye H,Ruskone-Fourmestraux A,Streubel B,Chott A,Raderer M,Gong L,Wlodarska I,De Wolf-Peeters C,MacLennan KA,de Leval L,Isaacson PG,Du MQ

    更新日期：2010-08-01 00:00:00

  • Minimally differentiated acute myeloid leukemia in Taiwan: predominantly occurs in children less than 3 years and adults between 51 and 70 years.

    abstract：:Acute myeloid leukemia (AML) with minimal differentiation was usually referred to as acute undifferentiated leukemia in the past. With the help of immunophenotyping, this subtype of leukemia was shown to express myeloid antigens on the blasts and was designated AML-M0 by FAB Cooperative Study Group in 1991. Among the ...

    journal_title：Leukemia

    pub_type： 临床试验,杂志文章

    doi：10.1038/sj.leu.2401521

    authors： Huang SY,Tang JL,Jou ST,Tsay W,Hu CH,Lin DT,Lin KS,Lin KS,Wang CH,Chen YC,Shen MC,Tien HF

    更新日期：1999-10-01 00:00:00

  • Incomplete DJH rearrangements of the IgH gene are frequent in multiple myeloma patients: immunobiological characteristics and clinical implications.

    abstract：:DH-JH rearrangements of the Ig heavy-chain gene (IGH) occur early during B-cell development. Consequently, they are detected in precursor-B-cell acute lymphoblastic leukemias both at diagnosis and relapse. Incomplete DJH rearrangements have also been occasionally reported in mature B-cell lymphoproliferative disorders...

    journal_title：Leukemia

    pub_type： 杂志文章

    doi：10.1038/sj.leu.2402964

    authors： González D,Balanzategui A,García-Sanz R,Gutiérrez N,Seabra C,van Dongen JJ,González M,San Miguel JF

    更新日期：2003-07-01 00:00:00

  • Distinct effects of ruxolitinib and interferon-alpha on murine JAK2V617F myeloproliferative neoplasm hematopoietic stem cell populations.

    abstract：:JAK2V617F is the most common mutation in patients with BCR-ABL negative myeloproliferative neoplasms (MPNs). The eradication of JAK2V617F hematopoietic stem cells (HSCs) is critical for achieving molecular remissions and cure. We investigate the distinct effects of two therapies, ruxolitinib (JAK1/2 inhibitor) and int...

    journal_title：Leukemia

    pub_type： 杂志文章

    doi：10.1038/s41375-019-0638-y

    authors： Austin RJ,Straube J,Bruedigam C,Pali G,Jacquelin S,Vu T,Green J,Gräsel J,Lansink L,Cooper L,Lee SJ,Chen NT,Lee CW,Haque A,Heidel FH,D'Andrea R,Hill GR,Mullally A,Milsom MD,Bywater M,Lane SW

    更新日期：2020-04-01 00:00:00

  • ProMACE-cytaBOM versus MACOP-B in intermediate and high grade NHL. Preliminary results of a prospective randomized trial.

    abstract：:One hundred seventy-nine patients with intermediate or high-grade non-Hodgkin's lymphoma were randomized to receive either ProMACE-CytaBOM (P-C) or MACOP-B (M-B). At last follow-up 71 patients in the P-C arm and 78 in the M-B arm were assessable for response. Forty-one patients treated with P-C (58%) and 49 patients t...

    journal_title：Leukemia

    pub_type： 临床试验,杂志文章,随机对照试验

    doi：

    authors： Federico M,Moretti G,Gobbi PG,Avanzini P,Cavanna L,Carotenuto M,Lombardo M,Leone G,Pitini V,Abbadessa V

    更新日期：1991-01-01 00:00:00

  • Pulmonary leukostasis secondary to all-trans retinoic acid in the treatment of acute promyelocytic leukemia in first relapse.

    abstract：:All-trans retinoic acid has recently been shown to induce differentiation of acute promyelocytic leukemia cells in vitro and in vivo. Clinical trials of patients treated de novo, in first relapse, and with resistant disease have achieved a high rate of complete remission (CR). The overall toxicity is substantially les...

    journal_title：Leukemia

    pub_type： 杂志文章

    doi：

    authors： Vosburgh E

    更新日期：1992-06-01 00:00:00

  • Heteroduplex PCR analysis of rearranged T cell receptor genes for clonality assessment in suspect T cell proliferations.

    abstract：:Molecular analysis of T cell receptor (TCR) genes is frequently used to prove or exclude clonality and thereby support the diagnosis of suspect T cell proliferations. PCR techniques are more and more being used for molecular clonality studies. The main disadvantage of the PCR-based detection of clonal TCR gene rearran...

    journal_title：Leukemia

    pub_type： 杂志文章

    doi：10.1038/sj.leu.2400887

    authors： Langerak AW,Szczepański T,van der Burg M,Wolvers-Tettero IL,van Dongen JJ

    更新日期：1997-12-01 00:00:00

  • 14-3-3ζ binds the proteasome, limits proteolytic function and enhances sensitivity to proteasome inhibitors.

    abstract：:14-3-3 proteins are a family of master regulators of intracellular signaling, yet their impact on proteasome function is unknown. We demonstrate that 14-3-3ζ binds the 11S proteasome activator, limiting proteasome assembly and cellular capacity for protein degradation. To define the functional impact of 14-3-3ζ protea...

    journal_title：Leukemia

    pub_type： 杂志文章

    doi：10.1038/leu.2017.288

    authors： Gu Y,Xu K,Torre C,Samur M,Barwick BG,Rupji M,Arora J,Neri P,Kaufman J,Nooka A,Bernal-Mizrachi L,Vertino P,Sun SY,Chen J,Munshi N,Fu H,Kowalski J,Boise LH,Lonial S

    更新日期：2018-03-01 00:00:00

  • Current status of reduced-intensity allogeneic stem cell transplantation using alternative donors.

    abstract：:The optimal donor for a patient undergoing reduced-intensity stem cell transplantation remains a human leukocyte antigen (HLA)-matched relative. Alternative donors such as matched unrelated donors (MUDs), mismatched related donors (haploidentical), or unrelated umbilical cord blood (UCB) units have emerged as options ...

    journal_title：Leukemia

    pub_type： 杂志文章,评审

    doi：10.1038/sj.leu.2404932

    authors： Chen YB,Spitzer TR

    更新日期：2008-01-01 00:00:00

  • CD34(+) or CD34(-): which is the more primitive?

    abstract：:Remarkable progress has been achieved in the characterization and isolation of primitive hematopoietic stem cells (HSC). HSC represent a very small subset of hematopoietic cells and provide self-renewal, possess differentiation capacity and allow a constant supply of the entire hematopoietic cell spectrum. Until recen...

    journal_title：Leukemia

    pub_type： 杂志文章,评审

    doi：10.1038/sj.leu.2402620

    authors： Engelhardt M,Lübbert M,Guo Y

    更新日期：2002-09-01 00:00:00

  • Acute myeloblastic leukemia with t(8;21) following Philadelphia positive acute lymphoblastic leukemia.

    abstract：:A patient with Philadelphia positive (Ph'+) acute lymphoblastic leukemia (ALL), in remission for over 4 years, developed an acute myeloblastic leukemia (AML), M2-type. During the second disease, the blast cells displayed a typical t(8;21)(q22;q22) translocation, in the absence of the Ph' chromosome. This is the first ...

    journal_title：Leukemia

    pub_type： 杂志文章

    doi：

    authors： De Cuia MR,Alimena G,Gastaldi R,Spiriti MA,Giona F,Mancini M,Mandelli F

    更新日期：1989-04-01 00:00:00

  • Stroma-conditioned media improve expansion of human primitive hematopoietic stem cells and progenitor cells.

    abstract：:It has been reported that stroma-dependent cultures support proliferation of hematopoietic stem cells (HSC). In order to investigate the effect of soluble stromal factors, we developed short-term serum-low liquid cultures in which the effect of stroma-conditioned media (SCM) from the murine FBMD-1, and human L87/4 and...

    journal_title：Leukemia

    pub_type： 杂志文章

    doi：10.1038/sj.leu.2400530

    authors： Breems DA,Blokland EA,Ploemacher RE

    更新日期：1997-01-01 00:00:00

  • Idarubicin DNA intercalation is reduced by MRP1 and not Pgp.

    abstract：:Currently available data regarding the substrate specificity of the multi-drug resistance (MDR) mechanisms P-glycoprotein (Pgp) and MDR-associated protein (MRP1) for idarubicin are inconclusive. A multiparameter flow cytometry method was developed which allows simultaneous quantitative measurement of total cellular fl...

    journal_title：Leukemia

    pub_type： 杂志文章

    doi：10.1038/sj.leu.2401496

    authors： Smeets ME,Raymakers RA,Vierwinden G,Pennings AH,Boezeman J,Minderman H,de Witte TM

    更新日期：1999-09-01 00:00:00

  • The TLR1/2 agonist PAM(3)CSK(4) instructs commitment of human hematopoietic stem cells to a myeloid cell fate.

    abstract：:Toll-like receptors (TLRs) constitute a family of nonpolymorphic receptors that are devoted to pathogen recognition. In this work, we have explored the impact of TLR ligands (TLR-L) on human hematopoietic stem cells (HSCs) and hematopoietic progenitor cells (HPCs). We show that HSCs and HPCs have a comparable pattern ...

    journal_title：Leukemia

    pub_type： 杂志文章

    doi：10.1038/leu.2009.155

    authors： De Luca K,Frances-Duvert V,Asensio MJ,Ihsani R,Debien E,Taillardet M,Verhoeyen E,Bella C,Lantheaume S,Genestier L,Defrance T

    更新日期：2009-11-01 00:00:00

  • Unexpected heterogeneity of PML/RAR alpha fused mRNA detected by nested polymerase chain reaction in acute promyelocytic leukemia.

    abstract：:We have analyzed ten APL patients using reverse transcription polymerase chain reaction (RT-PCR) technique to detect PML/RAR alpha fused mRNA. All patients in this study had PML/RAR alpha fused mRNA (three cases of the short type and seven cases of the long type), although the chromosomal translocation t(15;17) was no...

    journal_title：Leukemia

    pub_type： 杂志文章

    doi：

    authors： Matsuoka A,Miyamura K,Emi N,Tahara T,Tanimoto M,Naoe T,Ohno R,Kakizuka A,Evans RM,Saito H

    更新日期：1993-08-01 00:00:00