Abstract:
OBJECTIVE:This review focuses on commercial and in-house-developed reverse-transcription polymerase chain reaction (RT-PCR) assays used for the detection of enteroviral infections. In addition to providing details on the performance of RT-PCR, its specificity, and sensitivity, the clinical utility of this diagnostic method with specific reference to its impact on hospitalization and cost savings is addressed. DATA SOURCES:MEDLINE was searched for reports relating to RT-PCR detection of the enteroviruses in adults and children. The search was restricted to studies reported in English language journals. STUDY SELECTION:Reports documenting detailed information regarding the RT-PCR conditions, primers, sensitivity, specificity and, if relevant, clinical impact were selected for analysis. DATA EXTRACTION:Details regarding method of extraction of the enteroviral genome, the primers used, RT-PCR conditions, and sensitivity and specificity of the assay were extracted from the literature. For reports detailing the use of RT-PCR in the clinical management of enteroviral infections in children, the reduction in duration of hospitalization and health care cost savings were recorded. DATA SYNTHESIS:Reverse-transcription PCR can increase the yield of detection of enteroviruses from cerebrospinal fluid by a mean of approximately 20% over tissue culture. Reverse-transcription PCR of cerebrospinal fluid has been shown to exhibit sensitivity and specificity values of 86% to 100% and 92% to 100%, respectively. Reductions of 1 to 3 days of hospitalization per patient are predicted if RT-PCR is used to diagnose enteroviral meningitis in children. CONCLUSIONS:Reverse-transcription PCR detection of enteroviral infections is an extremely rapid, sensitive, and specific diagnostic modality. Both commercial assays and assays developed in-house appear to be equivalent with regard to sensitivity and specificity. Reverse-transcription PCR diagnosis of enteroviral infections in children could reduce the length of hospitalization and result in significant health care cost savings.
journal_name
Arch Pathol Lab Medjournal_title
Archives of pathology & laboratory medicineauthors
Romero JRdoi
10.1043/0003-9985(1999)123<1161:RTPCRD>2.0.CO;2subject
Has Abstractpub_date
1999-12-01 00:00:00pages
1161-9issue
12eissn
0003-9985issn
1543-2165journal_volume
123pub_type
杂志文章,评审abstract:CONTEXT:Plasma cell myeloma and chronic lymphocytic leukemia are both common hematologic malignancies, sharing many epidemiologic features. Concomitant detection of the 2 conditions poses special diagnostic challenges for the pathologist. OBJECTIVE:To describe the pathologic findings in cases of concomitant bone marro...
journal_title:Archives of pathology & laboratory medicine
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abstract::We describe 20 adult patients with malignant lymphoma with primary presentation in the spleen. The most common presenting symptoms were fever, malaise, and weight loss. Physical examination revealed prominent splenomegaly without palpable lymphadenopathy. Small lymphocytic lymphoma was the most frequent histologic typ...
journal_title:Archives of pathology & laboratory medicine
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更新日期:1985-12-01 00:00:00
abstract::In this time of significant change in pathology and medicine, pathology's future is uncertain. Many issues, including the emergence of molecular medicine, the demand for personalized medicine, confusing payment schemes, increasing governmental regulation, medical education challenges, administrative relationships, and...
journal_title:Archives of pathology & laboratory medicine
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journal_title:Archives of pathology & laboratory medicine
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abstract::Pulmonary and renal infection developed in a 44-year-old Hispanic man due to the fungus Pseudallescheria boydii. Pseudallescheria boydii has been associated with cutaneous infection known as mycetoma but occurs very infrequently in extracutaneous sites. To our knowledge, this is the second reported case of P boydii in...
journal_title:Archives of pathology & laboratory medicine
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journal_title:Archives of pathology & laboratory medicine
pub_type: 杂志文章
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journal_title:Archives of pathology & laboratory medicine
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abstract::At autopsy in the past three years, a new complex of CNS lesions has appeared in 54% (50/93) of the brains of premature infant cadavers that have come from our neonatal intensive care unit. This complex consists of necrotizing lesions in the periventricular white matter and in the gray nuclei of the cerebral hemispher...
journal_title:Archives of pathology & laboratory medicine
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journal_title:Archives of pathology & laboratory medicine
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journal_title:Archives of pathology & laboratory medicine
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journal_title:Archives of pathology & laboratory medicine
pub_type: 杂志文章
doi:
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journal_title:Archives of pathology & laboratory medicine
pub_type: 杂志文章
doi:
更新日期:1981-04-01 00:00:00
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journal_title:Archives of pathology & laboratory medicine
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doi:
更新日期:1997-12-01 00:00:00
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journal_title:Archives of pathology & laboratory medicine
pub_type: 杂志文章,评审
doi:
更新日期:1993-12-01 00:00:00
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journal_title:Archives of pathology & laboratory medicine
pub_type: 杂志文章
doi:
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journal_title:Archives of pathology & laboratory medicine
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journal_title:Archives of pathology & laboratory medicine
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journal_title:Archives of pathology & laboratory medicine
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更新日期:1982-02-01 00:00:00
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更新日期:2000-06-01 00:00:00
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