Abstract:
:The Xenopus oocyte expression and recording system has allowed a detailed analysis of the physiology and pharmacology of neuronal ion channels including their sensitivity to ethanol. It is important however, to ascertain the effects of a particular drug on the channels inherently expressed by oocytes to ensure that drug effects ascribed to the expressed recombinant receptors are manifested solely through those receptors. In this study, the effects of ethanol were determined on three endogenous currents that can be elicited in oocytes and other cells by various manipulations. The inward cation current, IC, was activated by perfusing naive oocytes with a divalent-free recording solution. Ethanol (25-100 mM) modestly inhibited IC with 100 mM ethanol producing a 7-8% inhibition of steady state currents. The store-operated or capacitative calcium current (I(SOC)) was activated in thapsigargin-treated oocytes by switching from a calcium-free solution to one containing 10 mM calcium. In thapsigargin-treated oocytes also injected with EGTA to block calcium-activated chloride currents, ethanol (100 mM) had no effect on the store-operated calcium current. In contrast, ethanol (10-100 mM) dose-dependently inhibited the calcium-dependent chloride current (I(Cl(Ca)) in thapsigargin-treated oocytes. A voltage-jump protocol was used to separate the two components of I(Cl(Ca)), I(Cl-1) and I(Cl-2). Under these conditions, ethanol (100 mM) inhibited I(Cl-1) currents to a greater extent (38%) than it did I(Cl-2) currents (14%). These results show that Xenopus oocytes express endogenous ion channels that are differentially sensitive to ethanol.
journal_name
Neurochem Intjournal_title
Neurochemistry internationalauthors
Clayton R,Woodward JJdoi
10.1016/s0197-0186(99)00097-2subject
Has Abstractpub_date
2000-01-01 00:00:00pages
67-74issue
1eissn
0197-0186issn
1872-9754pii
S0197-0186(99)00097-2journal_volume
36pub_type
杂志文章abstract::Na+-dependent uptake of excitatory neurotransmitter glutamate in astrocytes increases cell energy demands primarily due to the elevated ATP consumption by glutamine synthetase and Na+, K+-ATPase. The major pool of GLAST/EAAT1, the only glutamate transporter subtype expressed by human fetal astrocytes in undifferentiat...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/j.neuint.2006.12.015
更新日期:2007-06-01 00:00:00
abstract::Recent studies have suggested that neurosteroids such as pregnenolone, progesterone (PG) and their derivatives, have a role in activating autophagy in addition to diverse other functions. In our previous studies, we demonstrated that cellular free Zn(2+) is involved in oxidative stress-induced autophagy and autophagic...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/j.neuint.2011.11.015
更新日期:2012-01-01 00:00:00
abstract:BACKGROUND AND METHODS:Glycine receptor (GlyR) consists of two α (1-4) and three β subunits. Considerable evidence indicates that the adult retina expresses the four types of α subunits; however, the proportion of these subunits in adult and immature retina is almost unknown. In this report we have studied mRNA and the...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/j.neuint.2017.03.013
更新日期:2017-09-01 00:00:00
abstract::Malfunctioning of high-affinity glutamate transporters is believed to contribute to the accumulation of toxic concentrations of glutamate and, thus, trigger the cellular mechanisms of neurodegeneration. Emerging data point to the presence of excitotoxic component in Alzheimer's disease (AD) and aberrant expression of ...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/s0197-0186(03)00023-8
更新日期:2003-09-01 00:00:00
abstract::It was previously reported that the K+-evoked release of somatostatin-like immunoreactivity (SRIF-LI) and of cholecystokinin-like immunoreactivity (CCK-LI) from superfused rat cerebrocortical synaptosomes can be enhanced by NMDA or D-serine alone. We here studied the effects of extraterminal pH changes on SRIF-LI and ...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/j.neuint.2004.03.005
更新日期:2004-10-01 00:00:00
abstract::Increased noradrenaline, induced by rapid eye movement (REM) sleep deprivation, stimulates Na-K ATPase activity in the rat brain. The brain contains neurons as well as glia and both possess Na-K ATPase, however, it was not known if REM sleep deprivation affects the enzyme in both types of cells identically. Rats were ...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/j.neuint.2008.10.006
更新日期:2009-01-01 00:00:00
abstract::One hour of ischemia significantly increased protein S100B release from rat brain slices without altering lactate dehydrogenase leakage. Reoxygenation of the ischemic slices, however, increased the levels of these biochemical markers in the medium. Although removal of extracellular Ca(+2) ions from the medium did not ...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/j.neuint.2005.06.009
更新日期:2005-12-01 00:00:00
abstract::Several metabolic transformations of vitamin A have now been found in the pigment epithelium of cattle retina, leading to the hypothesis that there may be a vitamin A cycle in these cells. A retinol binding protein of MW ? 17,000 in pigment epithelial cytosol has been identified independently in several laboratories. ...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/0197-0186(80)90055-8
更新日期:1980-01-01 00:00:00
abstract::Aquaporin-4 (AQP4) is a key molecule for maintaining water and ion homeostasis associated with neuronal activity in the central nervous system, but the roles of AQP4 in adult neurogenesis remain largely unexplored. Based on preliminary evidences over the past years, AQP4 appears to emerge as an important player regula...
journal_title:Neurochemistry international
pub_type: 杂志文章,评审
doi:10.1016/j.neuint.2010.01.014
更新日期:2010-04-01 00:00:00
abstract::Glycine transporter GlyT1 plays important role in maintaining accurate glycine concentration in local brain microenvironment. Transporting efficiency of GlyT1 is strongly affected by the state of its distal C-terminus, which regulates transporter trafficking and cellular surface density. Using selected range of antibo...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/j.neuint.2010.06.003
更新日期:2010-10-01 00:00:00
abstract::Iron accumulation is considered to be involved in the pathogenesis of Parkinson's disease. To demonstrate the relationship between peripheral iron overload and dopaminergic neuron loss in rat substantia nigra (SN), in the present study we used fast cyclic voltammetry, tyrosine hydroxylase (TH) immunohistochemistry, Pe...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/j.neuint.2007.03.006
更新日期:2007-07-01 00:00:00
abstract::In the present investigation we address the question of whether one of the responses to increased neuronal activity is a modification of the expression of the different subunits of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA)-selective glutamate receptors (GluR-1, GluR-2, GluR-3). Thus, we used two dif...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/0197-0186(94)90144-9
更新日期:1994-10-01 00:00:00
abstract::Rapid eye movement sleep deprivation is associated with an increase in Na-K ATPase activity. In order to understand the possible biochemical mechanism of this increase, the kinetics of Na-K ATPase was studied. Although the enzyme activity increased after the deprivation, the catalytic efficiency of the enzyme remained...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/s0197-0186(99)00121-7
更新日期:2000-03-01 00:00:00
abstract::Cerebellar granule neurons produce homogenous cultures that provide a unique opportunity for quantifying the apoptosis by propidium iodide- and deoxynucleotidyl transferase-flow cytometry combined analysis and for studying its regulation by neurotrophins. Nerve growth factor (NGF) was found to promote postmitotic surv...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/s0197-0186(96)00147-7
更新日期:1997-08-01 00:00:00
abstract::In this review, we summarize the role of hyperglycemia during cerebral ischemia. Hyperglycemia occurring during experimental and clinical stroke has been associated with increased cerebral damage. Increased oxidative stress resulting from hyperglycemia is believed to contribute to the exacerbated damage. More specific...
journal_title:Neurochemistry international
pub_type: 杂志文章,评审
doi:10.1016/j.neuint.2007.03.002
更新日期:2007-06-01 00:00:00
abstract::Oxidative stress-induced neuronal death due to hydrogen peroxide overload plays a critical role in the pathogenesis of numerous neurological diseases. Insulin-like growth factor-1 (IGF-1) is important in maintaining neuronal survival, proliferation, and differentiation in the central nervous system. We now report that...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/j.neuint.2007.04.005
更新日期:2007-11-01 00:00:00
abstract::The system of GABA transporters in neural cells constitutes an efficient mechanism for terminating inhibitory GABAergic neurotransmission. As such these transporter are important therapeutical targets in epilepsy and potentially other neurological diseases related to the GABA system. In this study a number of analogs ...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/s0197-0186(03)00033-0
更新日期:2003-09-01 00:00:00
abstract:BACKGROUND:and purpose: In this study, we employed a multiple microinfarction (MMI) based vascular dementia (VaD) model in aged rats and tested the therapeutic effects of Sildenafil, a phosphodiesterase type 5 inhibitor, on cognitive decline, white matter damage, autophagy and inflammatory response associated with VaD....
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/j.neuint.2018.12.015
更新日期:2019-07-01 00:00:00
abstract::Primary cultures of cerebral cortical astrocytes and neurons, as well as neurons growing on top of the astrocytes (sandwich co-cultures), were incubated with 1-[13C]glucose or 2-[13C]acetate and in the presence or absence of the glutamine synthetase inhibitor methionine sulfoximine. [13C]NMR spectroscopy at 125 MHz wa...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/0197-0186(93)90064-c
更新日期:1993-01-01 00:00:00
abstract::Differentiating granule cells develop survival requirements in vitro which can be met by treatment with high K+ or excitatory amino acids. Promotion of cell survival by N-methyl-D-aspartate (NMDA) or kainate has already been established and here we report that treatment of the cells with alpha-amino-3-hydroxy-5-methyl...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/0197-0186(94)90067-1
更新日期:1994-09-01 00:00:00
abstract::In the present study, we investigated the neuroprotective role of p38 inhibition on experimental stroke in rats. p38 inhibition treatment alleviated the brain infarction volume and neurological deficits following ischemia, promoted the activation of Extracellular signal-regulated kinases (ERK1/2), suppressed the activ...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/j.neuint.2015.06.010
更新日期:2015-11-01 00:00:00
abstract::Human glutamate dehydrogenase (GDH) gene was fused with a gene fragment encoding the nine amino acid (RKKRRQRRR) protein transduction domain of human immunodeficiency virus TAT protein in bacterial expression vector to produce genetic in-frame TAT-GDH fusion protein. The TAT-GDH protein can enter PC12 cells efficientl...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/s0197-0186(01)00138-3
更新日期:2002-07-01 00:00:00
abstract::The possible influence of cholinergic and dopaminergic mechanisms on neurotensin-containing neurones was examined at two different levels; nucleus accumbens and striatum in the rat brain. The acute treatment with the anticholinergic drugs atropine and scopolamine increased neurotensin concentrations in the striatum an...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/0197-0186(88)90145-3
更新日期:1988-01-01 00:00:00
abstract::There is substantial evidence that hyperammonemia is one of the main factors contributing to the neurological alterations found in hepatic encephalopathy. The mechanisms by which chronic moderate hyperammonemia affects brain function involves alterations in neurotransmission at different steps. This article reviews th...
journal_title:Neurochemistry international
pub_type: 杂志文章,评审
doi:10.1016/s0197-0186(02)00030-x
更新日期:2002-08-01 00:00:00
abstract::Multiple sclerosis (MS) is a chronic autoimmune inflammatory disease of the central nervous system (CNS) affecting more than 2.5 million individuals worldwide. In the present study, myelin oligodendrocyte glycoprotein (MOG)-induced experimental autoimmune encephalomyelitis (EAE) mice were treated with adenosine recept...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/j.neuint.2019.104490
更新日期:2019-10-01 00:00:00
abstract::Changes in the level of ornithine decarboxylase mRNA after partial mesodiencephalic hemitransection were evaluated in various regions of the rat brain by means of in situ hybridization histochemistry coupled with computer-assisted image analysis. On days 1 and 2 after the lesion, increased accumulation of ornithine de...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/0197-0186(91)90165-a
更新日期:1991-01-01 00:00:00
abstract::Serotonin (5-HT) is a prime candidate for studies of the interaction between the nervous and immune systems, since it is both an important neurotransmitter and released at high concentrations at sites of inflammation. Serotonergic neurotransmission is regulated by the 5-HT transporter (5-HTT), which determines the mag...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/s0197-0186(98)00026-6
更新日期:1998-09-01 00:00:00
abstract::Cytokines are a heterogenous group of polypeptide mediators that have been associated with activation of numerous functions, including the immune system and inflammatory responses. The cytokine families include, but are not limited to, interleukins (IL-I alpha, IL-I beta, ILIra and IL-2-IL-15), chemokines (IL-8/ NAP-I...
journal_title:Neurochemistry international
pub_type: 杂志文章,评审
doi:10.1016/s0197-0186(96)00071-x
更新日期:1997-04-01 00:00:00
abstract::The mechanisms of intracellular calcium store depletion and store-related Ca(2+) dysregulation in relation to apoptotic cell death in PC12 cells were investigated at physiological temperatures with a leak-resistant fluorescent indicator dye Fura-PE3/AM by a cooled CCD imaging analysis system. Electron microscopic obse...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/j.neuint.2005.12.012
更新日期:2006-06-01 00:00:00
abstract::5,7-Dihydroxytryptamine (5,7-DHT) is a selective serotonergic neurotoxin by virtue of its selective uptake into 5-hydroxytryptamine neurons and its ability to undergo autoxidation. The mechanism by which 5,7-DHT induces neurodegenerative effects remains enigmatic. The mechanism of autoxidation of 5,7-DHT, which has be...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/0197-0186(88)90165-9
更新日期:1988-01-01 00:00:00