Cryptic epitopes on alpha-fetoprotein induce spontaneous immune responses in hepatocellular carcinoma, liver cirrhosis, and chronic hepatitis patients.

Abstract:

:To determine alpha-fetoprotein (AFP) immunogenicity in vivo, the presence of antibodies in sera of 60 hepatocellular carcinoma, 15 liver cirrhosis, and 15 chronic hepatitis patients was evaluated by Western blotting and immunoprecipitation analyses using purified human AFP. High titers of anti-AFP immunoglobulins were detected in 14 hepatocellular carcinomas (P = 0.0006), 3 liver cirrhosis (P = 0.0173), and 1 chronic hepatitis patient, but they were not detected in 40 healthy individuals. Therefore, spontaneous immune responses to AFP are significantly associated to liver diseases (P = 0.0015). Patient immunoglobulins recognized proteic linear epitopes that were cryptic in the native protein, as demonstrated by their restricted reactivity with denatured deglycosylated AFP. Thus, in pathological liver conditions, tolerance to this self-molecule is circumvented. The identification of AFP immunogenic epitopes may contribute to defining novel immunotherapeutic strategies targeting this antigen.

journal_name

Cancer Res

journal_title

Cancer research

authors

Bei R,Budillon A,Reale MG,Capuano G,Pomponi D,Budillon G,Frati L,Muraro R

subject

Has Abstract

pub_date

1999-11-01 00:00:00

pages

5471-4

issue

21

eissn

0008-5472

issn

1538-7445

journal_volume

59

pub_type

杂志文章
  • Breast cancer lung metastasis requires expression of chemokine receptor CCR4 and regulatory T cells.

    abstract::Cancer metastasis is a leading cause of cancer morbidity and mortality. More needs to be learned about mechanisms that control this process. In particular, the role of chemokine receptors in metastasis remains controversial. Here, using a highly metastatic breast cancer (4T1) model, we show that lung metastasis is a f...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:10.1158/0008-5472.CAN-08-4619

    authors: Olkhanud PB,Baatar D,Bodogai M,Hakim F,Gress R,Anderson RL,Deng J,Xu M,Briest S,Biragyn A

    更新日期:2009-07-15 00:00:00

  • Human papillomavirus type 16 E6 and E7 cooperate to increase epidermal growth factor receptor (EGFR) mRNA levels, overcoming mechanisms by which excessive EGFR signaling shortens the life span of normal human keratinocytes.

    abstract::Epidermal growth factor receptor (EGFR) levels are dramatically increased in human keratinocytes (HKc) immortalized with full-length human papillomavirus type 16 (HPV16) DNA (HKc/HPV16), but increases in EGFR levels actually precede immortalization. In some normal HKc strains, acute expression of HPV16 E6 (but not HPV...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Akerman GS,Tolleson WH,Brown KL,Zyzak LL,Mourateva E,Engin TS,Basaraba A,Coker AL,Creek KE,Pirisi L

    更新日期:2001-05-01 00:00:00

  • Membrane lipids and enzymes of cultured high- and low-metastatic B16 melanoma variants.

    abstract::B16 melanoma cell variants were used to determine if the metastatic properties of these cells could be correlated to distinct plasma membrane, microsome, and mitochondrial membrane lipid compositions and membrane-bound enzyme activities in high- and low-metastatic cell variants, respectively. The high-metastatic B16-F...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Schroeder F,Gardiner JM

    更新日期:1984-08-01 00:00:00

  • Transformation of chicken chondrocytes by Rous sarcoma virus.

    abstract::Chicken chondrocytes isolated from 11-day-old chicken vertebrate cartilage were transformed by Rous sarcoma virus ts LA24 of the Prague strain as well as by the wild-type Prague strain of Rous sarcoma virus. The morphology of chondrocytes transformed by Rous sarcoma virus ts LA 24 was dependent on the temperature, and...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Okayama M,Yoshimura M,Muto M,Chi J,Roth S,Kaji A

    更新日期:1977-03-01 00:00:00

  • PML/RARα-Regulated miR-181a/b Cluster Targets the Tumor Suppressor RASSF1A in Acute Promyelocytic Leukemia.

    abstract::In acute promyelocytic leukemia (APL), all-trans retinoic acid (ATRA) treatment induces granulocytic maturation and complete remission of leukemia. microRNAs are known to be critical players in the formation of the leukemic phenotype. In this study, we report downregulation of the miR-181a/b gene cluster in APL blasts...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:10.1158/0008-5472.CAN-14-3521

    authors: Bräuer-Hartmann D,Hartmann JU,Wurm AA,Gerloff D,Katzerke C,Verga Falzacappa MV,Pelicci PG,Müller-Tidow C,Tenen DG,Niederwieser D,Behre G

    更新日期:2015-08-15 00:00:00

  • Immune and clinical responses in patients with metastatic melanoma to CD34(+) progenitor-derived dendritic cell vaccine.

    abstract::Immunization to multiple defined tumor antigens for specific immune therapy of human cancer has thus far proven difficult. Eighteen HLA A*0201(+) patients with metastatic melanoma received injections s.c. of CD34(+)progenitor-derived autologous dendritic cells (DCs), which included Langerhans cells. DCs were pulsed wi...

    journal_title:Cancer research

    pub_type: 临床试验,杂志文章

    doi:

    authors: Banchereau J,Palucka AK,Dhodapkar M,Burkeholder S,Taquet N,Rolland A,Taquet S,Coquery S,Wittkowski KM,Bhardwaj N,Pineiro L,Steinman R,Fay J

    更新日期:2001-09-01 00:00:00

  • Carcinogenesis in human skin grafted to SCID mice.

    abstract::To directly examine a multistage carcinogenesis model and the role of UV light in human tissues, we grafted human skin onto mice with severe combined immunodeficiency disease. We found that the maximum dose of UV radiation in the B range (UVB; 280-320 nm) tolerated by these grafts was 500 J/m2 three times weekly. One ...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Soballe PW,Montone KT,Satyamoorthy K,Nesbit M,Herlyn M

    更新日期:1996-02-15 00:00:00

  • A retroinhibition approach reveals a tumor cell-autonomous response to rapamycin in head and neck cancer.

    abstract::Emerging evidence supporting the activation of the Akt-mammalian target of rapamycin (mTOR) signaling network in head and neck squamous cell carcinoma (HNSCC) progression has provided the rationale for exploring the therapeutic potential of inhibiting this pathway for HNSCC treatment. Indeed, rapamycin, a clinically r...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:10.1158/0008-5472.CAN-07-1756

    authors: Amornphimoltham P,Patel V,Leelahavanichkul K,Abraham RT,Gutkind JS

    更新日期:2008-02-15 00:00:00

  • Supplemental calcium suppresses colonic mucosal ornithine decarboxylase activity in elderly patients with adenomatous polyps.

    abstract::Epidemiological and animal studies suggest a role for calcium in the chemoprevention of colorectal neoplasia. This study was designed to investigate whether supplemental oral calcium has a suppressant effect on colonic mucosal ornithine decarboxylase (ODC) and tyrosine kinase activities in patients with adenomatous po...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Lans JI,Jaszewski R,Arlow FL,Tureaud J,Luk GD,Majumdar AP

    更新日期:1991-07-01 00:00:00

  • Cancer cachexia syndrome developed in nude mice bearing melanoma cells producing leukemia-inhibitory factor.

    abstract::Melanoma-derived lipoprotein lipase inhibitor (MLPLI) is a factor purified from the conditioned medium of a human melanoma cell line, SEKI, which induced severe cachexia in tumor-bearing nude mice. Amino acid sequencing revealed that the amino-terminal portion was identical to that of leukemia-inhibitory factor (LIF)....

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Mori M,Yamaguchi K,Honda S,Nagasaki K,Ueda M,Abe O,Abe K

    更新日期:1991-12-15 00:00:00

  • Hematopoietic Age at Onset of Triple-Negative Breast Cancer Dictates Disease Aggressiveness and Progression.

    abstract::Triple-negative breast cancer (TNBC) is considered an early onset subtype of breast cancer that carries with it a poorer prognosis in young rather than older women for reasons that remain poorly understood. Hematopoiesis in the bone marrow becomes altered with age and may therefore affect the composition of tumor-infi...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:10.1158/0008-5472.CAN-15-3332

    authors: Marsh T,Wong I,Sceneay J,Barakat A,Qin Y,Sjödin A,Alspach E,Nilsson B,Stewart SA,McAllister SS

    更新日期:2016-05-15 00:00:00

  • Overexpression of glycine-extended gastrin inhibits parietal cell loss and atrophy in the mouse stomach.

    abstract::Recently we have reported synergistic effects between glycine-extended gastrin (G-gly) and amidated gastrin-17 on acid secretion in short-term infusion studies. In the present study, we examined the long-term effect of G-gly on the atrophy-promoting effects of amidated gastrin in the mouse stomach with or without Heli...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:10.1158/0008-5472.CAN-04-0876

    authors: Cui G,Koh TJ,Chen D,Zhao CM,Takaishi S,Dockray GJ,Varro A,Rogers AB,Fox JG,Wang TC

    更新日期:2004-11-15 00:00:00

  • S100A10 Is a Critical Mediator of GAS6/AXL-Induced Angiogenesis in Renal Cell Carcinoma.

    abstract::Angiogenesis is a hallmark of cancer that promotes tumor progression and metastasis. However, antiangiogenic agents have limited efficacy in cancer therapy due to the development of resistance. In clear cell renal cell carcinoma (ccRCC), AXL expression is associated with antiangiogenic resistance and poor survival. He...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:10.1158/0008-5472.CAN-19-1366

    authors: Xiao Y,Zhao H,Tian L,Nolley R,Diep AN,Ernst A,Fuh KC,Miao YR,von Eyben R,Leppert JT,Brooks JD,Peehl DM,Giaccia AJ,Rankin EB

    更新日期:2019-11-15 00:00:00

  • Separation and characterization of transcriptionally active and inactive nuclear subfractions of AKR mouse embryo cells.

    abstract::This study was initiated with the objective of separating and characterizing two or more nuclear subfractions, which could then be compared with respect to their relative propensity for binding carcinogenic polycyclic aromatic hydrocarbons. Nuclei were isolated from cloned AKR-2B mouse embryo cells, which are suscepti...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Webster RA,Moses HL,Spelsberg TC

    更新日期:1976-08-01 00:00:00

  • Fluorodeoxyuridine modulates cellular expression of the DNA base excision repair enzyme uracil-DNA glycosylase.

    abstract::The thymidylate synthase inhibitor 5-fluorouracil (5-FU) continues to play a pivotal role in the treatment of cancer. A downstream event of thymidylate synthase inhibition involves the induction of a self-defeating base excision repair process. With the depletion of TTP pools, there is also an increase in dUMP. Metabo...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:10.1158/0008-5472.CAN-06-0540

    authors: Fischer JA,Muller-Weeks S,Caradonna SJ

    更新日期:2006-09-01 00:00:00

  • Effects of cryptorchidy, parabiosis, and estrogen administration upon Leydig cell tumorigenesis in Fischer rats.

    abstract::The effects on Leydig cell tumorigenesis of surgical cryptorchidy, parabiotic union with a castrate male partner, and a combination on the two procedures as well as of the continuous exposure to a high level of diethylstilbestrol stimulation were studied in the high-tumor Fischer rat. Localized foci of adenomatous hyp...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Huseby RA

    更新日期:1981-08-01 00:00:00

  • Cytoplasmic localization of the oncogenic protein Ski in human cutaneous melanomas in vivo: functional implications for transforming growth factor beta signaling.

    abstract::The oncogenic protein Ski associates with Smad proteins and counteracts their activation of gene expression and growth inhibition in response to transforming growth factor beta (TGF-beta). Here we show that Ski protein levels are increased in all 44 human melanoma tumor tissues analyzed in vivo. In addition, Ski subce...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Reed JA,Bales E,Xu W,Okan NA,Bandyopadhyay D,Medrano EE

    更新日期:2001-11-15 00:00:00

  • Nuclear factor-kappaB, an unappreciated tumor suppressor.

    abstract::The notion that nuclear factor-kappaB (NF-kappaB) is a tumor-promoting transcription factor has become a widely accepted dogma in biology. However, recent findings suggest an inhibitory role for NF-kappaB in carcinogenesis and tumorigenesis. Although the tumor suppressor-like effect of NF-kappaB remains to be rigorous...

    journal_title:Cancer research

    pub_type: 杂志文章,评审

    doi:10.1158/0008-5472.CAN-07-1576

    authors: Chen F,Castranova V

    更新日期:2007-12-01 00:00:00

  • Notch pathway inhibition depletes stem-like cells and blocks engraftment in embryonal brain tumors.

    abstract::The Notch signaling pathway is required in both nonneoplastic neural stem cells and embryonal brain tumors, such as medulloblastoma, which are derived from such cells. We investigated the effects of Notch pathway inhibition on medulloblastoma growth using pharmacologic inhibitors of gamma-secretase. Notch blockade sup...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:10.1158/0008-5472.CAN-06-0858

    authors: Fan X,Matsui W,Khaki L,Stearns D,Chun J,Li YM,Eberhart CG

    更新日期:2006-08-01 00:00:00

  • Classification of Epstein-Barr virus-positive gastric cancers by definition of DNA methylation epigenotypes.

    abstract::Epstein-Barr virus (EBV) is associated with Burkitt lymphoma, nasopharyngeal carcinoma, opportunistic lymphomas in immunocompromised hosts, and a fraction of gastric cancers. Aberrant promoter methylation accompanies human gastric carcinogenesis, though the contribution of EBV to such somatic methylation changes has n...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:10.1158/0008-5472.CAN-11-1349

    authors: Matsusaka K,Kaneda A,Nagae G,Ushiku T,Kikuchi Y,Hino R,Uozaki H,Seto Y,Takada K,Aburatani H,Fukayama M

    更新日期:2011-12-01 00:00:00

  • CD22 antigen is broadly expressed on lung cancer cells and is a target for antibody-based therapy.

    abstract::Most patients with lung cancer still die from their disease, necessitating additional options to improve treatment. Here, we provide evidence for targeting CD22, a cell adhesion protein known to influence B-cell survival that we found is also widely expressed in lung cancer cells. In characterizing the antitumor activ...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:10.1158/0008-5472.CAN-12-0173

    authors: Tuscano JM,Kato J,Pearson D,Xiong C,Newell L,Ma Y,Gandara DR,O'Donnell RT

    更新日期:2012-11-01 00:00:00

  • Combined modality therapy with bleomycin, hyperthermia, and radiation.

    abstract::In an attempt to develop better combination therapies for use with local radiation, the interaction between bleomycin and hyperthermia +/- radiation was studied in the FSaIIC tumor system. In cells exposed in vitro to bleomycin at 37 degrees C and at pH 7.40, the drug was substantially more toxic toward normally oxyge...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Teicher BA,Herman TS,Holden SA

    更新日期:1988-11-15 00:00:00

  • Topological control of p21WAF1/CIP1 expression in normal and neoplastic tissues.

    abstract::The p53-regulated gene product p21WAF1/CIP1 is the prototype of a family of small proteins that negatively regulate the cell cycle. To learn more about p21WAF1/CIP1 regulation in vivo, monoclonal antibodies were developed for immunohistochemistry. These revealed that p21WAF1/CIP1 expression followed radiation-induced ...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: el-Deiry WS,Tokino T,Waldman T,Oliner JD,Velculescu VE,Burrell M,Hill DE,Healy E,Rees JL,Hamilton SR

    更新日期:1995-07-01 00:00:00

  • Roscovitine inhibits STAT5 activity and induces apoptosis in the human leukemia virus type 1-transformed cell line MT-2.

    abstract::T cells expressing human leukemia virus (HTLV) type 1, the etiological agent of adult T-cell leukemia, are remarkably resistant to conventional chemotherapy, and the need for drugs that effectively kill these cells is apparent. Here we show that roscovitine, an inhibitor of cyclin-dependent kinases (CDKs), induces the...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Mohapatra S,Chu B,Wei S,Djeu J,Epling-Burnette PK,Loughran T,Jove R,Pledger WJ

    更新日期:2003-12-01 00:00:00

  • Polymorphism in the N-acetyltransferase 1 (NAT1) polyadenylation signal: association of NAT1*10 allele with higher N-acetylation activity in bladder and colon tissue.

    abstract::Exposures to carcinogens present in the diet, in cigarette smoke, or in the environment have been associated with increased risk of bladder and colorectal cancer. The aromatic amines and their metabolites, a class of carcinogen implicated in these exposures, can be N- or O-acetylated by the NAT1 and NAT2 enzymes. Acet...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Bell DA,Badawi AF,Lang NP,Ilett KF,Kadlubar FF,Hirvonen A

    更新日期:1995-11-15 00:00:00

  • Metastatic Progression of Prostate Cancer Is Mediated by Autonomous Binding of Galectin-4-O-Glycan to Cancer Cells.

    abstract::Metastatic prostate cancer continues to pose a difficult therapeutic challenge. Prostate cancer progression is associated with aberrant O-glycosylation of cancer cell surface receptors, but the functional impact of such events is uncertain. Here we report spontaneous metastasis of human prostate cancer xenografts that...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:10.1158/0008-5472.CAN-16-0641

    authors: Tsai CH,Tzeng SF,Chao TK,Tsai CY,Yang YC,Lee MT,Hwang JJ,Chou YC,Tsai MH,Cha TL,Hsiao PW

    更新日期:2016-10-01 00:00:00

  • Response of solid tumors to chemotherapy monitored by in vivo 31P nuclear magnetic resonance spectroscopy: a review.

    abstract::In vivo 31P nuclear magnetic resonance (MR) spectroscopy has shown great promise as a tool for cancer research and the clinical management of solid tumors. It is now possible in some cases to integrate MR spectroscopy with routine MR imaging of the cancer patient, so that tissue identified as tumor on an MR image can ...

    journal_title:Cancer research

    pub_type: 杂志文章,评审

    doi:

    authors: Steen RG

    更新日期:1989-08-01 00:00:00

  • Alteration of lymphocyte microtubule assembly, cytotoxicity, and activation by the anticancer drug taxol.

    abstract::We studied the effect of the anticancer drug taxol on the cytotoxic mechanism of major histocompatibility complex nonrestricted lymphocytes and their activation with interleukin 2. Unseparated lymphocytes or highly enriched natural killer or T-cells were treated with 0.2-10 micrograms/ml of taxol for various times and...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Chuang LT,Lotzová E,Heath J,Cook KR,Munkarah A,Morris M,Wharton JT

    更新日期:1994-03-01 00:00:00

  • Different effects of concanavalin A and E-phytohemagglutinin upon lymphocyte glycosyltransferase activities.

    abstract::We have studied glycosyltransferase activities in human lymphocytes stimulated with the plant mitogens E-phytohemagglutinin or concanavalin A (Con A) and have compared the results with activities found in resting lymphocytes. Compared to resting lymphocytes, Con A-stimulated lymphocytes possess an enhanced capacity to...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Speckart SF,Boldt DH,Ryerson KL

    更新日期:1978-12-01 00:00:00

  • Tumor-associated membrane sialoglycoprotein on human small cell lung carcinoma identified by the IgG2a monoclonal antibody SWA20.

    abstract::A mouse IgG2a monoclonal antibody, SWA20, defining a tumor-associated cell surface antigen on small cell carcinoma of the lung (SCC) was generated. The reactivity of the antibody with cell lines was examined by indirect immunofluorescence staining and solid phase radioimmunoassay and the reactivity with tissues by imm...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Waibel R,O'Hara CJ,Smith A,Stahel RA

    更新日期:1988-08-01 00:00:00