Expression of the calcium-sensing receptor in pancreatic islet B-cells.

abstract：:HMGB proteins are abundant, non-histone proteins in eukaryotic chromatin. HMGB proteins contain one or two conserved "HMG boxes" and can be sequence-specific or nonspecific in their DNA binding. HMGB proteins cause strong DNA bending and bind preferentially to deformed DNAs. We wish to understand how HMGB proteins inc...

journal_title：Biochemical and biophysical research communications

authors： Zimmerman J,Maher LJ 3rd

更新日期：2008-06-20 00:00:00

abstract：:We reported previously that GRF stimulates release of neurotensin (NT) by a clonal line of rat C cells (44-2 C). We report here that GRF stimulates calcitonin (CT) and cAMP release in these cells. For release experiments, replicate cultures are incubated for 5-180 min in serum-free defined medium. CT, NT and cAMP are ...

journal_title：Biochemical and biophysical research communications

authors： Zeytin F,Brazeau P

更新日期：1984-09-17 00:00:00

abstract：:2,3 Dehydro-2-deoxy-N-acetyl-neuraminic acid (DNANA) competitively inhibits the neuraminidase activity of Hemagglutinin-neuraminidase (HN) from Sendai virus. The inhibition constant depends on the presence of the Fusion (F) protein, which is 30 microM in the presence of active F protein and 50 microM when the F protei...

journal_title：Biochemical and biophysical research communications

authors： Dallocchio F,Tomasi M,Bellini T

更新日期：1995-03-08 00:00:00

abstract：:Previous work has demonstrated that pre-treatment of platelets with phorbol esters that activate protein kinase C eg phorbol 12-myristate 13-acetate (PMA) results in an inhibition of inositol phospholipid breakdown and granule secretion induced by physiological agonists such as thrombin and collagen. In the present st...

journal_title：Biochemical and biophysical research communications

authors： Krishnamurthi S,Kakkar VV

更新日期：1988-11-15 00:00:00

abstract：:The relation between platelet-derived growth factor (PDGF)-induced smooth muscle cell migration, measured in Boyden chambers, and cellular arachidonic acid cascade was studied by using rat aortic smooth muscle cells. Partially purified PDGF stimulated cell migration significantly at a concentration of 1.33-133.0 micro...

journal_title：Biochemical and biophysical research communications

authors： Nakao J,Ito H,Chang WC,Koshihara Y,Murota S

更新日期：1983-05-16 00:00:00

abstract：:We investigated the molecular mechanism underlying the neuroprotective effect of theanine, a green tea component, using primary cultured rat cortical neurons, focusing on group I metabotropic glutamate receptors (mGluRs). Theanine and a group I mGluR agonist, DHPG, inhibited the delayed death of neurons caused by brie...

journal_title：Biochemical and biophysical research communications

authors： Nagasawa K,Aoki H,Yasuda E,Nagai K,Shimohama S,Fujimoto S

更新日期：2004-07-16 00:00:00

abstract：:We previously found a new endogenous factor in rat liver cytosol, named ATP-stimulated glucocorticoid-receptor translocation promoter (ASTP), that increased the binding of activated glucocorticoid-receptor to nuclei in the presence of ATP. We have cloned and sequenced a cDNA (2989 bp) encoding the ASTP protein. An ope...

journal_title：Biochemical and biophysical research communications

authors： Okamoto K,Hirano H,Isohashi F

更新日期：1993-06-30 00:00:00

abstract：:Amylin, an islet amyloid peptide secreted by the pancreatic beta cell, has been proposed as a humoral regulator of islet insulin secretion. Four separate preparations of amylin were tested for effects on hormone secretion in both freshly isolated and cultured rat islets and in HIT-T15, hamster insulinoma cells. With a...

journal_title：Biochemical and biophysical research communications

authors： Broderick CL,Brooke GS,DiMarchi RD,Gold G

更新日期：1991-06-28 00:00:00

abstract：:The peroxidase and FAD-containing monooxygenase activities of porcine thyroid subcellular preparations were measured and it was observed that FAD-containing monooxygenase activity was considerably lower than that of peroxidase. The end product of 1-methyl-2[14C]thioimidazole oxidation catalysed by thyroid peroxidase w...

journal_title：Biochemical and biophysical research communications

authors： Paterson JR,Hood HT,Skellern GG

更新日期：1983-10-31 00:00:00

abstract：:MicroRNA-125a exhibits an antiproliferative activity and is downregulated in several types of tumors, including hepatocellular carcinoma where it targets sirtuin-7, matrix metalloproteinase-11, and c-Raf. Another target of miR-125a is Lin28, a pluripotency factor that is generally undetectable in differentiated cells ...

journal_title：Biochemical and biophysical research communications

authors： Panella M,Mosca N,Di Palo A,Potenza N,Russo A

更新日期：2018-06-07 00:00:00

abstract：:Recurrent osteosarcoma is a chemotherapy-resistant disease. Individualized precision therapy is needed for this disease. Toward this goal, we have developed the patient-derived othotopic xenograft (PDOX) mouse model of all major cancer types including osteosarcoma. Synergistic efficacy of trabectedin (TRAB) and irinot...

journal_title：Biochemical and biophysical research communications

authors： Higuchi T,Miyake K,Oshiro H,Sugisawa N,Yamamoto N,Hayashi K,Kimura H,Miwa S,Igarashi K,Chawla SP,Bouvet M,Singh SR,Tsuchiya H,Hoffman RM

更新日期：2019-05-28 00:00:00

abstract：:TGFbeta is of crucial importance during transdifferentiation of resting retinoid-storing hepatic stellate cells (HSC) to extracellular matrix producing myofibroblasts (MFB) and consequently, inhibition of TGFbeta signal transduction is an effective means for preventing experimental fibrosis. We have shown that isolate...

journal_title：Biochemical and biophysical research communications

authors： Dooley S,Streckert M,Delvoux B,Gressner AM

更新日期：2001-05-11 00:00:00

abstract：:Fut8 (α1,6-Fucosyltransferase) heterozygous knock-out (Fut8(+/-)) mice had an increased influx of inflammatory cells into the lungs, and this was associated with an up-regulation of matrix metalloproteinases, MMP-2 and MMP-9, after treatment with porcine pancreatic elastase (PPE), exhibiting an emphysema-prone phenoty...

journal_title：Biochemical and biophysical research communications

authors： Kamio K,Yoshida T,Gao C,Ishii T,Ota F,Motegi T,Kobayashi S,Fujinawa R,Ohtsubo K,Kitazume S,Angata T,Azuma A,Gemma A,Nishimura M,Betsuyaku T,Kida K,Taniguchi N

更新日期：2012-07-20 00:00:00

abstract：:Although the regeneration of the adult liver depends on hepatic progenitor cells (HPCs), many uncertainties regarding hepatic regeneration in the injured liver remain. Trefoil factor family 1 (TFF1), a secretory protein predominantly expressed in the gastrointestinal tract, is responsible for mucosal restitution. Here...

journal_title：Biochemical and biophysical research communications

authors： Hayashi Y,Yamaguchi J,Kokuryo T,Ebata T,Yokoyama Y,Nagino M

更新日期：2018-11-17 00:00:00

abstract：:Calmodulin is phosphorylated by casein kinase II on Thr-79, Ser-81, Ser-101 and Thr-117. To determine the consensus sequences for casein kinase II in intact calmodulin, we examined casein kinase II-mediated phosphorylation of engineered calmodulins with 1-4 deletions in the central helical region (positions 81-84). To...

journal_title：Biochemical and biophysical research communications

authors： Sacks DB,Davis HW,Crimmins DL,Persechini A,McDonald JM

更新日期：1992-10-30 00:00:00

abstract：:Parathyroid hormone-related protein (PTHrP) is produced by various normal and neoplastic tissues. Even if the physiological function(s) of PTHrP is unclear, evidence suggests that the protein may participate in the local regulation of smooth muscle contractility. We show here that PTHrP is produced in endothelial cell...

journal_title：Biochemical and biophysical research communications

authors： Rian E,Jemtland R,Olstad OK,Endresen MJ,Grasser WA,Thiede MA,Henriksen T,Bucht E,Gautvik KM

更新日期：1994-01-28 00:00:00

abstract：:Elevated Na(+) concentration ([Na(+)]) in the cerebrospinal fluid (CSF) contributes to the development of salt-sensitive hypertension. CSF is formed by the choroid plexus (CP) in cerebral ventricles, and [Na(+)] in CSF is controlled by transporters in CP. Here, we examined the effect of high salt diet on the expressio...

journal_title：Biochemical and biophysical research communications

authors： Guo L,Meng J,Xuan C,Ge J,Sun W,O'Rourke ST,Sun C

更新日期：2015-05-29 00:00:00

abstract：:DNA fragmentation factors (DFF) form protein complexes consisting of nuclease DFF40/CAD and inhibitory chaperon DFF45/ICAD. Although activated caspase-3 has been shown to cleave DFF complexes with the release of active DFF40 and DNA fragmentation, the organ-specific mechanisms of DFF turnover during liver injury accom...

journal_title：Biochemical and biophysical research communications

authors： Xiang Y,Johnson EA,Zhang C,Huang G,Hayes RL,Wang KK,Svetlov SI

更新日期：2006-11-17 00:00:00

abstract：:A 135kDa DNA polymerase alpha lacking primase activity has been purified to near homogeneity from Coprinus meiotic tissues. The activity of the DNA polymerase was sensitive to aphidicolin and N-ethylmaleimide, but was insensitive to dideoxythymidine triphosphate. DNA synthesis was proceeded with a low processivity. Ne...

journal_title：Biochemical and biophysical research communications

authors： Sawado T,Sakaguchi K

更新日期：1997-03-17 00:00:00

abstract：:Intramolecularly quenched fluorogenic peptide substrates with the general sequence: DABCYL-Lys-Phe-Gly-Gly-Xxx-Ala-EDANS have been utilized to explore the effect of the hydrophobicity of amino acid side chains in the P2' position on the steady-state kinetic constants for papain catalyzed hydrolysis. The results demons...

journal_title：Biochemical and biophysical research communications

authors： García-Echeverría C,Rich DH

更新日期：1992-09-16 00:00:00

abstract：:We have previously reported that RhoE is overexpressed in the SGC7901/VCR cell line. However, the potential role of RhoE in the development of multidrug resistance of gastric cancer is unknown. In the present study, RhoE enhanced the resistance of SGC7901 cells to several kinds of antitumor drugs. RhoE overexpression ...

journal_title：Biochemical and biophysical research communications

authors： Li K,Lu Y,Liang J,Luo G,Ren G,Wang X,Fan D

更新日期：2009-02-06 00:00:00

abstract：:The filamentous fungus Aspergillus ochraceus TS produces an inducible microsomal cytochrome P-450 linked monooxygenase which is capable of hydroxylating benzo(a)pyrene in presence of O2 and NADPH. The addition of Benzo(a)pyrene, 3-Methyl cholanthrene, beta-Naphthoflavone and other aryl hydrocarbons during the inductio...

journal_title：Biochemical and biophysical research communications

authors： Dutta D,Ghosh DK,Mishra AK,Samanta TB

更新日期：1983-09-15 00:00:00

abstract：:Replication of mammalian chromosomes depends on the activation of a large number of origins of DNA replication distributed along the chromosomes. We have focused our attention on a human DNA region, named ARSH1, localized to chromosome 2, that had been previously shown to act as an episomal origin in the yeast Sacchar...

journal_title：Biochemical and biophysical research communications

authors： Hu L,Xu X,Valenzuela MS

更新日期：2004-01-23 00:00:00

abstract：:PAF (0.2 microM) induced maximal platelet aggregation in human PRP and [3H]-PAF (1-5 nM) binding to platelet membrane preparations had Kd value of 3.8 nM and Bmax of 200 fmoles/mg of protein. Without UV irradiation, a synthetic azido tetrahydrofuran derivative L662,025 was a reversible and competitive PAF-receptor ant...

journal_title：Biochemical and biophysical research communications

authors： Hussaini IM,Shen TY

更新日期：1989-05-30 00:00:00

abstract：:Two new human genes encoding putative G protein-coupled receptors were cloned from genomic DNA following a degenerate PCR strategy. The predicted amino acid sequences of the proteins encoded by these genes have the characteristic motifs of chemokine receptors. This prompted us to name these genes CKR-L1 and CKR-L3. Ne...

journal_title：Biochemical and biophysical research communications

authors： Zaballos A,Varona R,Gutiérrez J,Lind P,Márquez G

更新日期：1996-10-23 00:00:00

abstract：:The effect of tyrosine-phosphorylation on the conformation of three tyrosine-based immunoreceptor activation motifs, zeta(69-86), zeta(106-126), and zeta(138-155), located in the T cell receptor/CD3 zeta-chain was investigated. Circular dichroism and Fourier-transform infrared spectroscopy of the nonphosphorylated and...

journal_title：Biochemical and biophysical research communications

authors： Laczkó I,Hollósi M,Vass E,Hegedüs Z,Monostori E,Tóth GK

更新日期：1998-01-26 00:00:00

abstract：:The DNA-binding activity of a 42-kDa phosphoprotein from salivary gland cells and cultured epithelial cells of Chironomus tentans have been analyzed by the Southwestern technique. Both the salivary gland and the epithelial cell 42-kDa polypeptides were found to be single-stranded DNA-binding proteins. They bind to sin...

journal_title：Biochemical and biophysical research communications

authors： Stigare J,Egyhazi E

更新日期：1991-05-15 00:00:00

abstract：:The effect of silkworm hemolymph on baculovirus-induced insect cell apoptosis was investigated. The addition of silkworm hemolymph into the culture medium either before or during the baculovirus infection increased the host cell longevity; however, its addition after the infection was less effective. This can be expla...

journal_title：Biochemical and biophysical research communications

authors： Rhee WJ,Park TH

更新日期：2000-04-29 00:00:00

abstract：:The NS3 serine protase of Hepatitis C virus (HCV) requires NS4A protein as a cofactor for efficient cleavage at four sites in the nonstructural region. The cofactor activity has been mapped to the central hydrophobic region (aa 22-34) of this 54-amino-acid NS4A protein, and site-directed mutagenesis has identified alt...

journal_title：Biochemical and biophysical research communications

authors： Butkiewicz NJ,Yao N,Wright-Minogue J,Zhang R,Ramanathan L,Lau JY,Hong Z,Dasmahapatra B

更新日期：2000-01-07 00:00:00

abstract：:With the emergence of electrospray ionization mass spectrometry (ESI-MS), mass spectrometry is no longer restricted to the study of small, stable molecules, but has become a viable technique to study large biomolecules as well as noncovalent biomolecular complexes. ESI-MS has been used to study noncovalent interaction...

journal_title：Biochemical and biophysical research communications

authors： Veenstra TD

更新日期：1999-04-02 00:00:00