Abstract：
:An automated modification of the most-probable-number (MPN) technique has been developed for enumeration of phagotrophic protozoa. The method is based on detection of prey depletion in micro titre plates rather than on presence of protozoa. A transconjugant Pseudomonas fluorescens DR54 labelled with a luxAB gene cassette was constructed, and used as growth medium for the protozoa in the micro titre plates. The transconjugant produced high amounts of luciferase which was stable and allowed detection for at least 8 weeks. Dilution series of protozoan cultures and soil suspensions were inoculated into micro titre plates amended with a suspension of the transconjugant. After 45 days measurement of light emission allowed detection of individual wells in the titre plates, where protozoan grazing had removed the inoculated bacteria.
journal_name
J Microbiol Methodsjournal_title
Journal of microbiological methodsauthors
Ekelund F,Christensen S,Rønn R,Buhl E,Jacobsen CSdoi
10.1016/s0167-7012(99)00086-xsubject
Has Abstractpub_date
1999-11-01 00:00:00pages
177-82issue
3eissn
0167-7012issn
1872-8359pii
S0167-7012(99)00086-Xjournal_volume
38pub_type
杂志文章abstract：:Our understanding of the molecular mechanisms of bacterial infection and pathogenesis are disproportionally derived from a small number of well-characterised species and strains. One reason for this is the enormous time and resources required to develop a new organism into experimental system that can be interrogated ...
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