Akt phosphorylation site found in human caspase-9 is absent in mouse caspase-9.

Abstract:

:Caspase-9 is one caspase upstream of caspase-3 and its activation is stimulated by Apaf-1/cytochrome c and inhibited by Akt signals. BAD phosphorylation by Akt is an essential step for growth factor-mediated inhibition of caspase activation. Recently, it was shown that human caspase-9 is phosphorylated by Akt and that its protease activity is reduced. To clarify the molecular mechanism of regulation of caspase-9 activation in neuronal apoptosis, we isolated two alternative splicing products of mouse caspase-9, caspase-9L and caspase-9S, from a P19 embryonal carcinoma cell cDNA library. Curiously, the Akt phosphorylation sites and motifs found in human caspase-9 were absent in both mouse caspase-9L and -9S. Mouse caspase-9 was not phosphorylated by activated Akt in vitro. Reverse transcription polymerase chain reaction analysis showed that the absent Akt motif is not limited to caspase-9 expressed in P19 embryonal carcinoma cells but also occurs in caspase-9 expressed in mouse, rat, and monkey. These results suggest that inhibition of caspase-9 activation by Akt-dependent phosphorylation is not generalized across species.

authors

Fujita E,Jinbo A,Matuzaki H,Konishi H,Kikkawa U,Momoi T

doi

10.1006/bbrc.1999.1387

subject

Has Abstract

pub_date

1999-10-22 00:00:00

pages

550-5

issue

2

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(99)91387-6

journal_volume

264

pub_type

杂志文章
  • Surface plasmon resonance for measuring TBP-promoter interaction.

    abstract::A procedure was developed for real-time measurement of the interaction between an archaeal TATA-binding protein (TBP) with stress-gene promoters from the archaeon Methanosarcina mazeii using surface plasmon resonance (SPR), the BIACORE 3000 equipment, and the SA (streptavidin) Sensor Chip. Measurements were based on t...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/s0006-291x(02)02388-4

    authors: Conway de Macario E,Rudofsky UH,Macario AJ

    更新日期:2002-11-15 00:00:00

  • The phorbol ester TPA induces a translocation of the insulin sensitive glucose carrier (GLUT4) in fat cells.

    abstract::Insulin activates the glucose transport in isolated fat cells through a translocation of the insulin sensitive glucose carrier subtype (GLUT4) and by activation of glucose carriers in the plasma membrane. Protein kinase C stimulating phorbol esters are able to mimick partially the insulin effect on glucose transport. ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/0006-291x(90)91141-e

    authors: Vogt B,Mushack J,Seffer E,Häring HU

    更新日期:1990-05-16 00:00:00

  • Singlet oxygen production from the peroxidase catalyzed formation of styrene glutathione adducts.

    abstract::Recently, Stock et al. (J. Biol. Chem. 261, 15915-15922 [1986]) described a model enzyme system composed of horseradish peroxidase, hydrogen peroxide, phenol, glutathione and styrene. This system forms glutathione-styrene conjugates. Glutathione radicals and carbon-centered radicals are intermediates in this process. ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/0006-291x(89)92215-8

    authors: Kanofsky JR

    更新日期:1989-03-31 00:00:00

  • Insulin-dependent phosphatidylinositol 3'-kinase activity co-precipitates with insulin receptor in human circulating mononuclear cells.

    abstract::In order to establish a working cellular model for the study of post-receptor signaling events, insulin-dependent phosphatidylinositol 3' (PtdIns 3')-kinase activity was investigated in circulating mononuclear cells from normal subjects. The p85 alpha regulatory subunit of PtdIns 3'-kinase co-precipitated with activat...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1006/bbrc.1995.1494

    authors: Renard E,Grigorescu F,Lavabre C,Kahn CR

    更新日期:1995-04-06 00:00:00

  • A role for TNFα in intervertebral disc degeneration: a non-recoverable catabolic shift.

    abstract::This study examines the effect of TNFα on whole bovine intervertebral discs in organ culture and its association with changes characteristic of intervertebral disc degeneration (IDD) in order to inform future treatments to mitigate the chronic inflammatory state commonly found with painful IDD. Pro-inflammatory cytoki...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2013.02.034

    authors: Purmessur D,Walter BA,Roughley PJ,Laudier DM,Hecht AC,Iatridis J

    更新日期:2013-03-29 00:00:00

  • Circular dichroism and Fourier-transform infrared spectroscopic studies on T-cell epitopic peptide fragments of influenza virus hemagglutinin.

    abstract::Epitopic peptides representing the C-terminal (HA1) region of cleaved hemagglutinin of influenza virus from different serotypes were synthesized. Circular dichroism and Fourier-transform infrared spectroscopic data showed that peptides HS2 and HS3 have a predominantly alpha-helical conformation in trifluoroethanol. Re...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1006/bbrc.1993.1759

    authors: Holly S,Majer Z,Tóth GK,Váradi G,Rajnavölgyi E,Laczkó I,Hollósi M

    更新日期:1993-06-30 00:00:00

  • Vascular A10 cell membranes contain an endothelin metabolizing neutral endopeptidase.

    abstract::We have investigated the possible presence of endothelin-metabolizing neutral endopeptidase (NEP, EC 3.4.24.11) on A10 cell membranes using [125I]-ET-1 binding and direct measurements of NEP. NEP activity of A10 cell membranes has been compared to that of solubilized rat kidney brush border membranes (KNEP). Specific ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/0006-291x(91)90941-y

    authors: Dickinson KE,Tymiak AA,Cohen RB,Liu EC,Webb ML,Hedberg A

    更新日期:1991-04-15 00:00:00

  • Stimulus-induced phosphorylation of PKC theta at the C-terminal hydrophobic-motif in human T lymphocytes.

    abstract::Protein kinase C (PKC) is a family of serine/threonine kinases whose activity is controlled, in part, by phosphorylation on three conserved residues that are located on the catalytic domain of the enzyme, known as the activation-loop, the turn-motif, and the C-terminal hydrophobic-motif sites. Using a panel of phospho...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2005.06.136

    authors: Freeley M,Volkov Y,Kelleher D,Long A

    更新日期:2005-08-26 00:00:00

  • The presence of both growth inhibitory and growth stimulatory factors on membranes prepared from mouse liver.

    abstract::Plasma membrane fractions from mouse livers were examined for the presence of growth regulatory peptides. Both growth inhibitory and growth stimulatory factors were found on these membranes. The growth inhibitory component could be enriched by extractions with both dimethylmaleic anhydride and octylglucoside. The grow...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/s0006-291x(84)80191-6

    authors: Lieberman MA

    更新日期:1984-05-16 00:00:00

  • Both nitric oxide and nitrite prevent homocysteine-induced endoplasmic reticulum stress and subsequent apoptosis via cGMP-dependent pathway in neuronal cells.

    abstract::Growing evidence indicates that endoplasmic reticulum (ER) stress and/or ER stress-mediated apoptosis may play a role in the pathogenesis of neurodegenerative diseases such as Alzheimer's disease. The present study investigated the effects of non-cytotoxic concentrations of nitric oxide (NO) and nitrite, a metabolite ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2017.09.054

    authors: Jeong SO,Son Y,Lee JH,Choi SW,Kim SH,Cheong YK,Chung HT,Pae HO

    更新日期:2017-11-04 00:00:00

  • Effect of thyroid hormone on hepatic cholesterol 7 alpha hydroxylase, LDL receptor, HMG-CoA reductase, farnesyl pyrophosphate synthetase and apolipoprotein A-I mRNA levels in hypophysectomized rats.

    abstract::The effects of thyroid hormone on cholesterol 7 alpha hydroxylase, LDL receptor, HMG-CoA reductase, apo A-I and farnesyl pyrophosphate synthetase hepatic mRNA levels were investigated in hypophysectomized rats. Of these mRNAs cholesterol 7 alpha hydroxylase responded the most rapidly and required the lowest dose of T3...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/0006-291x(90)91568-d

    authors: Ness GC,Pendleton LC,Li YC,Chiang JY

    更新日期:1990-11-15 00:00:00

  • MicroRNA-187-3p mitigates non-small cell lung cancer (NSCLC) development through down-regulation of BCL6.

    abstract::Hsa-microRNA-187-3p (miR-187-3p) has recently been discovered having anticancer efficacy in different organs. However, the role of miR-187-3p on non-small cell lung cancer (NSCLC) is still ambiguous. In this study, we investigated the role of miR-187-3p on the development of NSCLC. The results indicated that miR-187-3...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2016.01.175

    authors: Sun C,Li S,Yang C,Xi Y,Wang L,Zhang F,Li D

    更新日期:2016-02-26 00:00:00

  • Structural and biochemical insights into inhibition of human primase by citrate.

    abstract::The eukaryotic primase/polymerase complex synthesizes approximately 107 primers, one per Okazaki fragment, during the replication of mammalian chromosomes, which contain 109 base pairs. Primase catalyzes the synthesis of a short RNA segment to a single-stranded DNA template. Primase is important in DNA replication bec...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2018.11.047

    authors: Lee JG,Park KR,An JY,Kang JY,Shen H,Wang J,Eom SH

    更新日期:2018-12-09 00:00:00

  • Joint cultivation of human erythroblastoid cells and mouse fibroblasts triggers release of a wide spectrum of cytotoxic factors.

    abstract::It has been demonstrated that joint cultivation of human K-562 cells and mouse L-929 cells results in triggering apoptosis in L-929 cells and possibly also in K-562 cells. Analysis of proteins recovered from the culture medium has revealed the presence of three cytotoxic factors including TNF-alpha. This is the first ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1006/bbrc.1997.6617

    authors: Gnuchev NV,Sashchenko LP,Iarovaia OV,Dukhanina E,Lukianova T,Razin SV

    更新日期:1997-05-29 00:00:00

  • Site directed mutagenesis of two cysteine residues in the E. coli ogt O6-alkylguanine DNA alkyltransferase protein.

    abstract::The E. coli ogt O6-alkylguanine-DNA alkyltransferase has two cysteine residues positioned identically with respect to cysteines in the E. coli ada O6-alkylguanine-DNA alkyltransferase. In order to assess their function, these residues were each substituted by a glycine to generate altered forms of the ogt protein. Mut...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/s0006-291x(05)81510-4

    authors: Harris LC,Potter PM,Margison GP

    更新日期:1992-08-31 00:00:00

  • Activation of Ca2+-stimulated ATPase by phospholipid N-methylation in cardiac sarcoplasmic reticulum.

    abstract::Incubation of cardiac sarcoplasmic reticulum (SR) in the presence of S-adenosyl-L-methionine, a methyl donor for the enzymatic N-methylation of phosphatidylethanolamine, increased Ca2+-stimulated ATPase activity. The increase in Ca2+-ATPase activity was not due to changes in the affinity for Ca2+ and was prevented by ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/0006-291x(85)90441-3

    authors: Ganguly PK,Panagia V,Okumura K,Dhalla NS

    更新日期:1985-07-16 00:00:00

  • Urotensin II increases foam cell formation by repressing ABCA1 expression through the ERK/NF-κB pathway in THP-1 macrophages.

    abstract:OBJECTIVE:Foam cell formation in the arterial wall plays a key role in the development of atherosclerosis. Recent studies showed that Urotensin II (U II) is involved in the pathogenesis of atherosclerosis. Here we examined the effects of human U II on ATP-binding cassette transporter A1 (ABCA1) expression and the under...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2014.09.030

    authors: Wang Y,Wu JF,Tang YY,Zhang M,Li Y,Chen K,Zeng MY,Yao F,Xie W,Zheng XL,Zeng GF,Tang CK

    更新日期:2014-10-03 00:00:00

  • Involvement of lysosomes in substrate stabilization of tryptophan-2,3-dioxygenase in rat liver.

    abstract::Administration of tryptophan or hydrocortisone to rats caused a several-fold increase in tryptophan-2,3-dioxygenase activity in the liver. Highly purified lysosomes were isolated from livers of tryptophan- or hydrocortisone-treated animals as well as the control rats. Immunoblotting of lysosomal proteins with anti-try...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/0006-291x(92)91644-6

    authors: Sato A,Endo Y,Natori Y

    更新日期:1992-02-28 00:00:00

  • Regulatory role of GM3 ganglioside in integrin function, as evidenced by its effect on function of alpha 5 beta 1-liposomes: a preliminary note.

    abstract::Mouse mammary carcinoma mutant cell line FUA169, characterized by high GM3 ganglioside content, was established from parent cell line FM3A/F28-7, which has high LacCer content but no GM3. Although both cell lines showed the same quantity and quality of integrin receptors, FUA169 showed much stronger adhesion to fibron...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/s0006-291x(05)81561-x

    authors: Zheng M,Tsuruoka T,Tsuji T,Hakomori S

    更新日期:1992-08-14 00:00:00

  • Increase in the relative abundance of preproenkephalin A messenger RNA in the ventricles of cardiomyopathic hamsters.

    abstract::Preproenkephalin A messenger RNA was detected in hamster heart by Northern blot analysis using a human preproenkephalin A cDNA probe. Ventricular levels of this messenger were one order of magnitude lower than atrial levels, which were equivalent to brain levels. Furthermore, in the heart of cardiomyopathic hamsters, ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/s0006-291x(88)81107-0

    authors: Ouellette M,Brakier-Gingras L

    更新日期:1988-08-30 00:00:00

  • WITHDRAWN: Expression of p53, p63, and p73 isoforms in squamous cell carcinoma and adenocarcinoma of esophagus☆,☆☆

    abstract::This article has been withdrawn consistent with Elsevier Policy on Article Withdrawal (http://www.elsevier.com/locate/withdrawalpolicy). The Publisher apologizes for any inconvenience this may cause. ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2005.08.084

    authors: Cui R,He J,Mei R,de Fromentel CC,Martel-Planche G,Taniere P,Hainaut P

    更新日期:2005-08-22 00:00:00

  • Human receptor for activated protein kinase C1 associates with polyglutamine aggregates and modulates polyglutamine toxicity.

    abstract::Formation of SDS-insoluble protein aggregates in affected neurons is a cellular pathological feature of polyglutamine (polyQ) disease. We identified a multi-WD-domain protein, receptor for activated protein kinase C1 (RACK1), as a novel polyQ aggregate component from a Drosophila transgenic polyQ disease model. We sho...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2008.10.057

    authors: Lam W,Chan WM,Lo TW,Wong AKY,Wu CC,Chan HYE

    更新日期:2008-12-12 00:00:00

  • Tetrahydro-sepiapterin is an intermediate in tetrahydrobiopterin biosynthesis.

    abstract::7,8-Dihydrobiopterin is not an intermediate in the de novo biosynthesis of tetrahydrobiopterin, the cofactor required for aromatic amino acid hydroxylations. However, N-acetyl-serotonin inhibition of sepiapterin reductase, an enzyme whose previously only known function was the reduction of sepiapterin to 7,8-dihydrobi...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/s0006-291x(83)80018-7

    authors: Milstien S,Kaufman S

    更新日期:1983-09-30 00:00:00

  • Functional characterization of malaria parasites deficient in the K+ channel Kch2.

    abstract::K+ channels are integral membrane proteins, which contribute to maintain vital parameters such as the cellular membrane potential and cell volume. Malaria parasites encode two K+ channel homologues, Kch1 and Kch2, which are well-conserved among members of the Plasmodium genus. In the rodent malaria parasite P. berghei...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2017.08.128

    authors: Ellekvist P,Mlambo G,Kumar N,Klaerke DA

    更新日期:2017-11-04 00:00:00

  • BRD4 suppression alleviates cerebral ischemia-induced brain injury by blocking glial activation via the inhibition of inflammatory response and pyroptosis.

    abstract::Ischemic stroke is a major cause of death and disability worldwide. Hyperneuroinflammation significantly contributes to ischemic stroke. Bromodomain-containing protein 4 (BRD4) is a member of the Bromo and Extra-Terminal (BET) family, and promotes inflammatory response in various types of tissue and cells. Thereby, we...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2019.07.097

    authors: Zhou Y,Gu Y,Liu J

    更新日期:2019-11-12 00:00:00

  • Enhanced detection and characterization of protocatechuate 3,4-dioxygenase in Acinetobacter lwoffii K24 by proteomics using a column separation.

    abstract::Acinetobacter lwoffii K24 known as an aniline degrading bacterium has also been found to utilize p-hydroxybenzoate as a sole carbon source. In this study, 2-DE using Q-Sepharose column separation was attempted for fast screening of protocatechuate 3,4-dioxygenase for catabolism of p-hydroxybenzoate in A. lwoffii K24. ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/s0006-291x(02)00778-7

    authors: Kahng HY,Cho K,Song SY,Kim SJ,Leem SH,Kim SI

    更新日期:2002-07-26 00:00:00

  • Identification of AHCY inhibitors using novel high-throughput mass spectrometry.

    abstract::S-adenosylhomocysteine hydrolase (AHCY) catalyzes the reversible hydrolysis of S-adenosylhomocysteine (SAH) to adenosine and l-homocysteine. This enzyme is frequently overexpressed in many tumor types and is considered to be a validated anti-tumor target. In order to enable the development of small molecule AHCY inhib...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2017.05.107

    authors: Uchiyama N,Dougan DR,Lawson JD,Kimura H,Matsumoto SI,Tanaka Y,Kawamoto T

    更新日期:2017-09-09 00:00:00

  • Enhanced inhibitory effects of a novel CpG motif on osteoclast differentiation via TREM-2 down-regulation.

    abstract::Recognition of oligodeoxynucleotides containing CpG motifs (CpG-ODNs) by toll-like receptor 9 (TLR9) inhibits RANKL-induced osteoclastogenesis from precursors. This inhibitory effect suggests the possibility of using this strategy to block pathological bone loss. However, the enhancing effect of CpG-ODNs on OC formati...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2009.08.080

    authors: Chang JH,Chang EJ,Kim HH,Kim SK

    更新日期:2009-11-06 00:00:00

  • RNase treatment of yeast and mammalian cell extracts affects in vitro substrate methylation by type I protein arginine N-methyltransferases.

    abstract::Type I protein arginine N-methyltransferases catalyze the formation of omega-NG-monomethylarginine and asymmetric omega-NG, NG-dimethylarginine residues using S-adenosyl-l-methionine as the methyl donor. In vitro these enzymes can modify a number of soluble methyl-accepting substrates in yeast and mammalian cell extra...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1006/bbrc.1999.0779

    authors: Frankel A,Clarke S

    更新日期:1999-06-07 00:00:00

  • Nonresponsiveness of immature B lymphocytes to anti-immunoglobulin is reversed by pronase.

    abstract::Splenic B cells are induced to proliferate upon culture with antibody having specificity for surface membrane immunoglobulins. Cells treated with pronase, washed and then cultured with antibody, exhibited a greater than 5-fold enhancement of DNA synthesis whereas pronase treatment, per se, was not mitogenic. The prona...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/0006-291x(84)91609-7

    authors: Gollapudi SV,Ramanadham M,Kern M

    更新日期:1984-02-29 00:00:00