Evidence of absence of porcine endogenous retrovirus (PERV) infection in patients treated with a bioartificial liver support system.

Abstract:

:Porcine endogenous retrovirus (PERV) genomes are present in all pig cells. In this retrospective study, we assessed PERV infectivity in 28 patients treated with an extracorporeal bioartificial liver (HepatAssist system) that includes a membrane device containing porcine hepatocytes. All patients tested negative for PERV using polymerase chain reaction analysis of peripheral blood mononuclear cells (PBMC) collected up to 5 years after treatment. In vitro results showed that the membrane decreased the risk of PERV transmission by a factor of 105, and porcine hepatocytes did not produce infectious PERV in co-cultures with human cell line 293. Our results do not support the presence of PERV infection in patients treated with this porcine hepatocyte-based bioartificial liver.

journal_name

Artif Organs

journal_title

Artificial organs

authors

Pitkin Z,Mullon C

doi

10.1046/j.1525-1594.1999.06444.x

subject

Has Abstract

pub_date

1999-09-01 00:00:00

pages

829-33

issue

9

eissn

0160-564X

issn

1525-1594

pii

6444

journal_volume

23

pub_type

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