Pluripotent stem cells isolated from human amniotic fluid and differentiation into pancreatic beta-cells.

Abstract:

:Human amniotic fluid (HAF) contains multipotent stem cells [amniotic fluid-derived stem cells (AFSC)] which can differentiate into a variety of different cell types. Recently, we demonstrated that obestatin, a peptide encoded by the ghrelin gene, exerts anti-apoptotic effects in pancreatic beta-cells and human islets and increases the expression of genes involved in beta-cells differentiation. We investigated whether: 1) AFSC would differentiate into pancreatic beta-cells and 2) obestatin would increase beta-cells differentiation from AFSC. Fluorescence-activated cell sorting analysis and immunocytochemical staining showed the presence of mesenchymal and endothelial markers in AFSC. Real-time PCR evidenced the expression of Octamer binding transcription factor 4 (OCT-4), a marker of pluripotency, during the early differentiation phase. However, the beta-cells differentiation marker duodenal homeobox factor-1 (PDX-1) could not be detected. Obestatin increased OCT-4 expression but had no effect on beta-cells differentiation. These results suggest that, at least under the experimental conditions used in this study, AFSC do not differentiate into beta-cells and obestatin has no additional effect.

journal_name

J Endocrinol Invest

authors

Trovato L,De Fazio R,Annunziata M,Sdei S,Favaro E,Ponti R,Marozio L,Ghigo E,Benedetto C,Granata R

doi

10.1007/BF03345764

subject

Has Abstract

pub_date

2009-12-01 00:00:00

pages

873-6

issue

11

eissn

0391-4097

issn

1720-8386

pii

6401

journal_volume

32

pub_type

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