Abstract:
:Human amniotic fluid (HAF) contains multipotent stem cells [amniotic fluid-derived stem cells (AFSC)] which can differentiate into a variety of different cell types. Recently, we demonstrated that obestatin, a peptide encoded by the ghrelin gene, exerts anti-apoptotic effects in pancreatic beta-cells and human islets and increases the expression of genes involved in beta-cells differentiation. We investigated whether: 1) AFSC would differentiate into pancreatic beta-cells and 2) obestatin would increase beta-cells differentiation from AFSC. Fluorescence-activated cell sorting analysis and immunocytochemical staining showed the presence of mesenchymal and endothelial markers in AFSC. Real-time PCR evidenced the expression of Octamer binding transcription factor 4 (OCT-4), a marker of pluripotency, during the early differentiation phase. However, the beta-cells differentiation marker duodenal homeobox factor-1 (PDX-1) could not be detected. Obestatin increased OCT-4 expression but had no effect on beta-cells differentiation. These results suggest that, at least under the experimental conditions used in this study, AFSC do not differentiate into beta-cells and obestatin has no additional effect.
journal_name
J Endocrinol Investjournal_title
Journal of endocrinological investigationauthors
Trovato L,De Fazio R,Annunziata M,Sdei S,Favaro E,Ponti R,Marozio L,Ghigo E,Benedetto C,Granata Rdoi
10.1007/BF03345764subject
Has Abstractpub_date
2009-12-01 00:00:00pages
873-6issue
11eissn
0391-4097issn
1720-8386pii
6401journal_volume
32pub_type
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