Abstract:
:We tested the endogenous tripeptide TRH (thyrotropin releasing hormone) ability to bind to MC (melanocortin) receptor subtypes. We discovered that TRH binds to the human MCI receptor expressed in COS cells and to murine B16 melanoma cells with 5790+/-1010 nM and 6370+/-1260 nM Ki's, respectively. TRH did not bind to the human MC3, MC4 or MC5 receptor subtypes. Moreover, TRH also stimulated cAMP production in murine B16 melanoma cells reaching the same maximum level of cAMP as found for alpha-MSH. However, several analogues of TRH, including TRH-OH, TRH-Gly-NH2 and other analogues, where each of the three amino acid residues in TRH had been exchanged by a related residue, did not bind to any of the MC receptors tested in this study. C(alpha) atoms of molecular models of TRH and the core of a MSH peptide were aligned with r.m.s. of 0.01 A. Moreover, TRH could be docked into a binding pocket of a molecular model of the MC1 receptor at only a little higher energy than a short cyclic MSH peptide. The data indicate a similarity in the mode of TRH and MSH activation of the MCI receptor.
journal_name
Peptidesjournal_title
Peptidesauthors
Schiöth HB,Prusis P,Muceniece R,Mutulis F,Mutule I,Wikberg JEdoi
10.1016/s0196-9781(98)00168-5subject
Has Abstractpub_date
1999-01-01 00:00:00pages
395-400issue
3eissn
0196-9781issn
1873-5169pii
S0196-9781(98)00168-5journal_volume
20pub_type
杂志文章相关文献
PEPTIDES文献大全abstract::Many animal studies show an enhancing effect of vasopressin (VP) on memory, but not all human studies could confirm this finding. This study examined the influence of post-learning administration of VP (40 IU, intranasally) on the consolidation of declarative memories in healthy humans during different intervals of sl...
journal_title:Peptides
pub_type: 临床试验,杂志文章
doi:10.1016/s0196-9781(01)00625-8
更新日期:2002-03-01 00:00:00
abstract::The temporins are a family of hydrophobic, C-terminally alpha-amidated antimicrobial peptides that are synthesized in the skins of a wide range of species of frogs belonging to the genus Rana. In the present study, we investigated using RT-PCR the expression of preprotemporin mRNAs in extradermal tissues of Tago's bro...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2006.03.023
更新日期:2006-09-01 00:00:00
abstract::Spinal cords of rats, cats and monkeys were transected; the animals were perfused at varying times. Other rats were injected with morphine and perfused 10 days later. Immunocytochemistry shows substance P (SP) present in control animals primarily in the substantia gelatinosa (SG) of the dorsal horn of the spinal cord....
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(81)90057-7
更新日期:1981-01-01 00:00:00
abstract::Sex pheromone biosynthesis in many moth species is controlled by a cerebral neuropeptide, termed pheromone biosynthesis activating neuropeptide (PBAN). PBAN is a 33 amino acid C-terminally amidated neuropeptide that is produced by neuroendocrine cells of the subesophageal ganglion (SEG). Studies of the regulation of s...
journal_title:Peptides
pub_type: 杂志文章,评审
doi:10.1016/j.peptides.2004.06.020
更新日期:2004-09-01 00:00:00
abstract::Recently, a new serine protease inhibitor family has been described in arthropods. Eight members were purified from locusts and 13 peptides have been identified by cDNA cloning. The peptides share a conserved cysteine array (Cys-Xaa(9-12)-Cys-Asn-Xaa-Cys-Xaa-Cys-Xaa(2-3)-Gly-Xaa(3-6)-Cys-Thr-Xaa(3)-Cys) with nine inhi...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2004.03.005
更新日期:2004-06-01 00:00:00
abstract::Generally, biomacromolecules, such as DNA, RNA, and proteins, cannot freely permeate into cells from outside the membrane. Protein transduction domains (PTDs) are peptides containing a large number of basic amino acids that can deliver macromolecules into living cells. Arginine-rich intracellular delivery (AID) peptid...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2009.06.006
更新日期:2009-09-01 00:00:00
abstract::Medium conditioned by a highly enriched population of gonadotrophs, cultured as reaggregates in the presence of 0.01 nM GnRH, was concentrated, separated on a reversed-phase HPLC column, and tested for activity on lactotroph development in pituitary reaggregate cell cultures of 14-day-old rats. The number of cells exp...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/s0196-9781(96)00175-1
更新日期:1996-01-01 00:00:00
abstract::VIP stimulates adenylate cyclase activity of male and female rat anterior pituitaries and human prolactinomas, while dopamine inhibits the enzyme activity of female rat pituitaries and prolactinomas. A dopamine inhibited cyclase can be detected also in male rats provided the enzyme activity is increased by VIP. The an...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(84)90172-4
更新日期:1984-11-01 00:00:00
abstract::beta-Lactotensin (beta-LT) is a bioactive peptide derived from bovine milk beta-lactoglobulin and is a natural ligand for neurotensin receptors. We examined the effect of beta-LT on restraint stress and fear memory in mice. Mice subjected to acute restraint stress exhibited a decreased number of head-dips and increase...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2006.08.009
更新日期:2006-12-01 00:00:00
abstract::Opiate alkaloids and opioid peptides have been shown to suppress plasma LH and FSH levels via a naloxone sensitive mechanism in several species including man. Three subtypes of opiate receptors have been characterized: mu, delta and kappa. The present study was designed to investigate their role in gonadotropin releas...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(83)90012-8
更新日期:1983-09-01 00:00:00
abstract::Pharmacological study of Phe-Met-Leu-Phe-amide (FMRFa) receptors is hindered by the lack of selective ligands. The classification of these selective ligands is further hampered by the limited availability of functional assays. In this study, we evaluated several synthetic FMRFa analogs for agonist and antagonist activ...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/s0196-9781(97)00481-6
更新日期:1998-01-01 00:00:00
abstract::The aim of the present study was to evaluate in hypothalamus and hindbrain of rainbow trout in vitro the effect of leptin treatment on glucosensing capacity and the expression of orexigenic and anorexigenic peptides involved in the control of food intake. In a first experiment, the response of parameters involved in g...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2010.11.007
更新日期:2011-02-01 00:00:00
abstract::High levels of neuropeptide Y (NPY) are found in the hypothalamus, median eminence, pituitary portal blood, and the pituitary in a number of species. Neuropeptide Y may influence the synthesis and secretion of a variety of hormones by interacting with specific receptors in the hypothalamus and/or the pituitary. To fur...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(94)90090-6
更新日期:1994-01-01 00:00:00
abstract::Endogenous hemorphins, derived from degradation of the beta-chain of hemoglobin, lower arterial blood pressure and exert an antinociceptive action in experimental models of nociception. Hemopressin, derived from the alpha-chain of hemoglobin, also decreases blood pressure, but its effects on pain have not been studied...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2004.10.026
更新日期:2005-03-01 00:00:00
abstract::To determine whether a sex difference exists in the biosynthetic capacity of vasopressingergic (AVP) neurons in the bed nucleus of the stria terminalis (BNST), we have used in situ hybridization and quantitative autoradiography to measure propressophysin messenger RNA levels in these cells from adult male and female r...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(89)90152-6
更新日期:1989-05-01 00:00:00
abstract::We labeled substance P (SP), neurokinin A (NKA), and [Lys0]gastrin-releasing peptide-27 (GRP) with cyanine 3.18 (cy3). Cy3-peptides purified by HPLC were fully active, determined by [Ca2+]i mobilization. Binding was specific because it was abolished by unlabeled peptides and receptor antagonists. Transfected cells yie...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(95)00042-i
更新日期:1995-01-01 00:00:00
abstract::A novel protease inhibitor, designated mungoin, with both antifungal and antibacterial activities, and exhibiting a molecular mass of 10kDa in SDS-polyacrylamide gel electrophoresis, was isolated from mung bean (Phaseolus mungo) seeds. The isolation procedure involved a combination of extraction, ammonium sulfate prec...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2006.07.013
更新日期:2006-12-01 00:00:00
abstract::Capsaicin-sensitive sensory nerves participate in the regulation of cardiovascular functions both in the normal state and the pathophysiology of hypertension through the actions of potent vasodilator neuropeptides, including calcitonin gene-related peptide (CGRP). CGRP, a very potent vasodilator, is the predominant ne...
journal_title:Peptides
pub_type: 杂志文章,评审
doi:10.1016/j.peptides.2005.02.002
更新日期:2005-09-01 00:00:00
abstract::Urinary tract infections (UTI) are important health problems and predisposing causes of UTI are not entirely known. Neuro-immune interactions play an important role in human health and disease. Capsaicin-sensitive sensory nerves which in nerve bladder extensively regulate immune system through neuropeptides such as su...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2014.04.003
更新日期:2014-06-01 00:00:00
abstract::The major active product of ghrelin gene is a 28-amino acid peptide acylated at the serine 3 position with an octanoyl group, called simply ghrelin. Ghrelin has a multiplicity of physiological functions, affecting GH release, food intake, energy and glucose homeostasis, gastrointestinal, cardiovascular, pulmonary and ...
journal_title:Peptides
pub_type: 杂志文章,评审
doi:10.1016/j.peptides.2008.02.018
更新日期:2008-07-01 00:00:00
abstract::The functional help provided by four cross-linked synthetic peptides from HIV-1 Gag structural proteins was investigated in the mouse model. These peptides, selected upon non-self-criteria, are not predicted as T epitopes by classical prediction methods such as the Rothbard consensus or the amphipathy rule. Priming mi...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(94)90055-8
更新日期:1994-01-01 00:00:00
abstract::N-hydroxysuccinimide (NHS) esters of biotin are reported to react specifically with amino groups of peptides and proteins. However, we have found that these reagents can readily acylate other functional groups in specific peptide sequences under relatively mild conditions. We have extended our inquiry of sequence-depe...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/s0196-9781(97)00225-8
更新日期:1997-01-01 00:00:00
abstract::Phe-Leu-Phe-Gln-Pro-Gln-Arg-Phe-NH2 (NPFF), an endogenous mammalian antiopioid peptide, has been shown by other laboratories to attenuate the acute antinociceptive effects of morphine, the development of morphine tolerance, and naloxone-induced withdrawal in morphine-dependent rats. The present study determined the ef...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(93)90187-l
更新日期:1993-11-01 00:00:00
abstract::Biological and immunological relationships between molt-inhibiting hormone (MIH) activity in eyestalk ganglia extracts of the crab, Cancer antennarius Stimpson, and peptides of the vasopressin-oxytocin family were assessed. Lysine vasopressin (LVP), arginine vasopressin (AVP), vasotocin (VT), and oxytocin (OT) mimicke...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(85)90166-4
更新日期:1985-07-01 00:00:00
abstract::Ceratotoxin A (CtxA), a 36-residue alpha-helical cationic peptide isolated from the medfly Ceratitis capitata, exhibits strong antibacterial activity. To determine its mode of action against bacteria, we investigated the behavior of ceratotoxin A by incorporating it into planar lipid bilayers. Macroscopic and single c...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2003.09.015
更新日期:2003-11-01 00:00:00
abstract::Urotensin-II (U-II), a ligand for the G-protein-coupled receptor UT, has been characterized as the most potent mammalian vasoconstrictor identified to date. Although circulating levels of U-II are altered in lower species (e.g., fish) upon exposure to hypo-osmotic stress, little is known about the actions of this cycl...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2005.10.004
更新日期:2006-06-01 00:00:00
abstract::Higher levels of natriuretic peptides were identified in outpatients after myocardial infarction (MI) compared to the healthy population, even in the absence of heart failure (HF). Therefore, we assessed the optimal cut-off value of B-type natriuretic peptide (BNP) in relation to new-onset HF prediction in 79 post-MI ...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2010.06.023
更新日期:2010-10-01 00:00:00
abstract::ACTH/MSH(4-10) (10 micrograms/kg/b.i.d.; IP), administered to pregnant Sprague-Dawley rats during gestational days (GD) 3 to 12, significantly increased the metabolic activity of extensor digitorum longus (EDL) muscle at postnatal day 14. ACTH/MSH peptide, administered from day of birth to postnatal day 13, had no eff...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(90)90128-r
更新日期:1990-01-01 00:00:00
abstract::The localization of CCK8-, bombesin- and VIP-like immunoreactivities in the myenteric plexus of the guinea pig small intestine has been studied by radioimmunoassay of extracts of longitudinal muscle strips obtained with and without adherent myenteric plexus; concentrations were compared with those in other regions of ...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/s0196-9781(81)80007-1
更新日期:1981-04-01 00:00:00
abstract::BMAP-27, a member of cathelicidin family, plays an important role against microorganisms, including bacteria and fungi. BMAP-27 may exert antimicrobial effects through membrane integrity disruption, but the exact molecular mechanism remains unclear. To identify the structural features important for antimicrobial activ...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2019.170106
更新日期:2019-08-01 00:00:00