当前位置： SCI文献检索 > MOLECULAR CELL期刊下所有文献 > A role for TBP dimerization in preventing unregulated gene expression.

A role for TBP dimerization in preventing unregulated gene expression.

Abstract：

:The recruitment of the TATA box-binding protein (TBP) to promoters in vivo is often rate limiting in gene expression. We present evidence that TBP negatively autoregulates its accessibility to promoter DNA in yeast through dimerization. The crystal structure of TBP dimers was used to design point mutations in the dimer interface. These mutants are impaired for dimerization in vitro, and in vivo they generate large increases in activator-independent gene expression. Overexpression of wild-type TBP suppresses these mutants, possibly by heterodimerizing with them. In addition to loss of autorepression, dimerization-defective TBPs are rapidly degraded in vivo. Direct detection of TBP dimers in vivo was achieved through chemical cross-linking. Taken together, the data suggest that TBP dimerization prevents unregulated gene expression and its own degradation.

journal_name

Mol Cell

journal_title

Molecular cell

authors

Jackson-Fisher AJ,Chitikila C,Mitra M,Pugh BF

doi

10.1016/s1097-2765(01)80004-6

subject

Has Abstract

pub_date

1999-06-01 00:00:00

pages

717-27

issue

6

eissn

1097-2765

issn

1097-4164

pii

S1097-2765(01)80004-6

journal_volume

3

pub_type

杂志文章

相关文献

MOLECULAR CELL文献大全
  • A Functional Mini-Integrase in a Two-Protein-type V-C CRISPR System.

    abstract：:CRISPR-Cas immunity requires integration of short, foreign DNA fragments into the host genome at the CRISPR locus, a site consisting of alternating repeat sequences and foreign-derived spacers. In most CRISPR systems, the proteins Cas1 and Cas2 form the integration complex and are both essential for DNA acquisition. M...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2018.12.015

    authors： Wright AV,Wang JY,Burstein D,Harrington LB,Paez-Espino D,Kyrpides NC,Iavarone AT,Banfield JF,Doudna JA

    更新日期：2019-02-21 00:00:00

  • TopBP1 interacts with BLM to maintain genome stability but is dispensable for preventing BLM degradation.

    abstract：:The Bloom syndrome helicase BLM and topoisomerase-IIβ-binding protein 1 (TopBP1) are key regulators of genome stability. It was recently proposed that BLM phosphorylation on Ser338 mediates its interaction with TopBP1, to protect BLM from ubiquitylation and degradation (Wang et al., 2013). Here, we show that the BLM-T...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2015.02.012

    authors： Blackford AN,Nieminuszczy J,Schwab RA,Galanty Y,Jackson SP,Niedzwiedz W

    更新日期：2015-03-19 00:00:00

  • Mitosis hit with an ATM transaction fee: aurora B-mediated activation of ATM during mitosis.

    abstract：:In this issue of Molecular Cell, Yang et al. (2011) demonstrate that Aurora B phosphorylates ATM, leading to its mitotic activation and ability to phosphorylate Bub1 and regulate the spindle checkpoint, thus maintaining genomic integrity. ...

    journal_title：Molecular cell

    pub_type： 评论,杂志文章

    doi：10.1016/j.molcel.2011.11.001

    authors： Bihani T,Hinds PW

    更新日期：2011-11-18 00:00:00

  • PARP-1 Activation Requires Local Unfolding of an Autoinhibitory Domain.

    abstract：:Poly(ADP-ribose) polymerase-1 (PARP-1) creates the posttranslational modification PAR from substrate NAD(+) to regulate multiple cellular processes. DNA breaks sharply elevate PARP-1 catalytic activity to mount a cell survival repair response, whereas persistent PARP-1 hyperactivation during severe genotoxic stress is...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2015.10.013

    authors： Dawicki-McKenna JM,Langelier MF,DeNizio JE,Riccio AA,Cao CD,Karch KR,McCauley M,Steffen JD,Black BE,Pascal JM

    更新日期：2015-12-03 00:00:00

  • Tissue-specific splicing of disordered segments that embed binding motifs rewires protein interaction networks.

    abstract：:Alternative inclusion of exons increases the functional diversity of proteins. Among alternatively spliced exons, tissue-specific exons play a critical role in maintaining tissue identity. This raises the question of how tissue-specific protein-coding exons influence protein function. Here we investigate the structura...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2012.05.039

    authors： Buljan M,Chalancon G,Eustermann S,Wagner GP,Fuxreiter M,Bateman A,Babu MM

    更新日期：2012-06-29 00:00:00

  • Partitioning of Chemotherapeutics into Nuclear Condensates-Opening the Door to New Approaches for Drug Development.

    abstract：:Klein et al. (2020) demonstrate for the first time that small-molecule cancer therapeutics are selectively partitioned and concentrated within phase-separated nuclear condensates, providing new insights to drug efficacy and creating the opportunity for enhanced control of therapeutic targeting. ...

    journal_title：Molecular cell

    pub_type： 评论,杂志文章

    doi：10.1016/j.molcel.2020.07.029

    authors： Howard TP,Roberts CWM

    更新日期：2020-08-20 00:00:00

  • Smad7 binds to Smurf2 to form an E3 ubiquitin ligase that targets the TGF beta receptor for degradation.

    abstract：:Ubiquitin-mediated proteolysis regulates the activity of diverse receptor systems. Here, we identify Smurf2, a C2-WW-HECT domain ubiquitin ligase and show that Smurf2 associates constitutively with Smad7. Smurf2 is nuclear, but binding to Smad7 induces export and recruitment to the activated TGF beta receptor, where i...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/s1097-2765(00)00134-9

    authors： Kavsak P,Rasmussen RK,Causing CG,Bonni S,Zhu H,Thomsen GH,Wrana JL

    更新日期：2000-12-01 00:00:00

  • Actin homolog MreB affects chromosome segregation by regulating topoisomerase IV in Escherichia coli.

    abstract：:In Escherichia coli, topoisomerase IV, a type II topoisomerase, mediates the resolution of topological linkages between replicated daughter chromosomes and is essential for chromosome segregation. Topo IV activity is restricted to only a short interval late in the cell cycle. However, the mechanism that confers this t...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2009.01.001

    authors： Madabhushi R,Marians KJ

    更新日期：2009-01-30 00:00:00

  • Insulin activation of Rheb, a mediator of mTOR/S6K/4E-BP signaling, is inhibited by TSC1 and 2.

    abstract：:Tumor suppressor genes evolved as negative effectors of mitogen and nutrient signaling pathways, such that mutations in these genes can lead to pathological states of growth. Tuberous sclerosis (TSC) is a potentially devastating disease associated with mutations in two tumor suppressor genes, TSC1 and 2, that function...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/s1097-2765(03)00220-x

    authors： Garami A,Zwartkruis FJ,Nobukuni T,Joaquin M,Roccio M,Stocker H,Kozma SC,Hafen E,Bos JL,Thomas G

    更新日期：2003-06-01 00:00:00

  • Transcriptional ShortCUTs.

    abstract：:Recent work from Kuehner and Brow (2008) and Thiebaut et al. (2008) in Molecular Cell and Jenks et al. (2008) in Molecular and Cellular Biology reveals that regulated expression of central nucleotide synthesis pathway components directs start site-dependent RNA polymerase II termination. ...

    journal_title：Molecular cell

    pub_type： 评论,杂志文章

    doi：10.1016/j.molcel.2008.08.014

    authors： Dichtl B

    更新日期：2008-09-05 00:00:00

  • Reciprocal association of the budding yeast ATM-related proteins Tel1 and Mec1 with telomeres in vivo.

    abstract：:The phosphoinositide (PI)-3-kinase-related kinase (PIKK) family proteins Tel1p and Mec1p have been implicated in the telomere integrity of Saccharomyces cerevisiae. However, the mechanism of PIKK-mediated telomere length control remains unclear. Here, we show that Tel1p and Mec1p are recruited to the telomeres at spec...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/s1097-2765(04)00262-x

    authors： Takata H,Kanoh Y,Gunge N,Shirahige K,Matsuura A

    更新日期：2004-05-21 00:00:00

  • The Histone Acetyltransferase Mst2 Protects Active Chromatin from Epigenetic Silencing by Acetylating the Ubiquitin Ligase Brl1.

    abstract：:Faithful propagation of functionally distinct chromatin states is crucial for maintaining cellular identity, and its breakdown can lead to diseases such as cancer. Whereas mechanisms that sustain repressed states have been intensely studied, regulatory circuits that protect active chromatin from inactivating signals a...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2017.05.026

    authors： Flury V,Georgescu PR,Iesmantavicius V,Shimada Y,Kuzdere T,Braun S,Bühler M

    更新日期：2017-07-20 00:00:00

  • Mechanism of UCH-L5 activation and inhibition by DEUBAD domains in RPN13 and INO80G.

    abstract：:Deubiquitinating enzymes (DUBs) control vital processes in eukaryotes by hydrolyzing ubiquitin adducts. Their activities are tightly regulated, but the mechanisms remain elusive. In particular, the DUB UCH-L5 can be either activated or inhibited by conserved regulatory proteins RPN13 and INO80G, respectively. Here we ...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2014.12.039

    authors： Sahtoe DD,van Dijk WJ,El Oualid F,Ekkebus R,Ovaa H,Sixma TK

    更新日期：2015-03-05 00:00:00

  • Identification of a BRCA1-mRNA splicing complex required for efficient DNA repair and maintenance of genomic stability.

    abstract：:Mutations within BRCA1 predispose carriers to a high risk of breast and ovarian cancers. BRCA1 functions to maintain genomic stability through the assembly of multiple protein complexes involved in DNA repair, cell-cycle arrest, and transcriptional regulation. Here, we report the identification of a DNA damage-induced...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2014.03.021

    authors： Savage KI,Gorski JJ,Barros EM,Irwin GW,Manti L,Powell AJ,Pellagatti A,Lukashchuk N,McCance DJ,McCluggage WG,Schettino G,Salto-Tellez M,Boultwood J,Richard DJ,McDade SS,Harkin DP

    更新日期：2014-05-08 00:00:00

  • Transcriptional activation: mediator can act after preinitiation complex formation.

    abstract：:The Mediator complex connects transcriptional activators bound at enhancers with RNA polymerase (Pol) II. demonstrate that Mediator also has an important role in transcriptional activation after recruitment of the Pol II machinery to promoters. ...

    journal_title：Molecular cell

    pub_type： 评论,杂志文章,评审

    doi：10.1016/j.molcel.2005.03.002

    authors： Struhl K

    更新日期：2005-03-18 00:00:00

  • Aurora A on the mitotic spindle is activated by the way it holds its partner.

    abstract：:An exciting study in this issue reveals how binding of the microtubule associated protein TPX2 to the mitotic kinase Aurora A induces a conformational change. This moves the phosphorylated activation domain into a more compact position within the kinase core, providing a better substrate binding platform and hiding th...

    journal_title：Molecular cell

    pub_type： 评论,杂志文章,评审

    doi：10.1016/s1097-2765(03)00405-2

    authors： Glover DM

    更新日期：2003-10-01 00:00:00

  • Codon Bias as a Means to Fine-Tune Gene Expression.

    abstract：:The redundancy of the genetic code implies that most amino acids are encoded by multiple synonymous codons. In all domains of life, a biased frequency of synonymous codons is observed at the genome level, in functionally related genes (e.g., in operons), and within single genes. Other codon bias variants include biase...

    journal_title：Molecular cell

    pub_type： 杂志文章,评审

    doi：10.1016/j.molcel.2015.05.035

    authors： Quax TE,Claassens NJ,Söll D,van der Oost J

    更新日期：2015-07-16 00:00:00

  • A role for Fkbp6 and the chaperone machinery in piRNA amplification and transposon silencing.

    abstract：:Epigenetic silencing of transposons by Piwi-interacting RNAs (piRNAs) constitutes an RNA-based genome defense mechanism. Piwi endonuclease action amplifies the piRNA pool by generating new piRNAs from target transcripts by a poorly understood mechanism. Here, we identified mouse Fkbp6 as a factor in this biogenesis pa...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2012.07.019

    authors： Xiol J,Cora E,Koglgruber R,Chuma S,Subramanian S,Hosokawa M,Reuter M,Yang Z,Berninger P,Palencia A,Benes V,Penninger J,Sachidanandam R,Pillai RS

    更新日期：2012-09-28 00:00:00

  • A new twist on clock protein phosphorylation: a conformational change leads to protein degradation.

    abstract：:Progressive phosphorylation of circadian clock proteins is a hallmark of time-keeping. In this issue of Molecular Cell, Querfurth et al. (2011) demonstrate that phosphorylation of Neurospora FRQ induces a conformational change, which can account for its temporally gated degradation. ...

    journal_title：Molecular cell

    pub_type： 评论,杂志文章

    doi：10.1016/j.molcel.2011.08.015

    authors： Menet JS,Rosbash M

    更新日期：2011-09-02 00:00:00

  • Impeding DNA Break Repair Enables Oocyte Quality Control.

    abstract：:Oocyte quality control culls eggs with defects in meiosis. In mouse, oocyte death can be triggered by defects in chromosome synapsis and recombination, which involve repair of DNA double-strand breaks (DSBs) between homologous chromosomes. We show that RNF212, a SUMO ligase required for crossing over, also mediates oo...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2018.08.031

    authors： Qiao H,Rao HBDP,Yun Y,Sandhu S,Fong JH,Sapre M,Nguyen M,Tham A,Van BW,Chng TYH,Lee A,Hunter N

    更新日期：2018-10-18 00:00:00

  • A transcriptional activator is part of an SCF ubiquitin ligase to control degradation of its cofactors.

    abstract：:Multisubunit protein complexes pose a challenge to the coordinated regulation of individual components. We show how the yeast transactivating factor Met4 functions as a component of the SCF(Met30) ubiquitin ligase to synchronize its own activity with cofactor assembly. Cells maintain Met4 in a dormant state by a regul...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2010.11.018

    authors： Ouni I,Flick K,Kaiser P

    更新日期：2010-12-22 00:00:00

  • Structures of Neisseria meningitidis Cas9 Complexes in Catalytically Poised and Anti-CRISPR-Inhibited States.

    abstract：:High-resolution Cas9 structures have yet to reveal catalytic conformations due to HNH nuclease domain positioning away from the cleavage site. Nme1Cas9 and Nme2Cas9 are compact nucleases for in vivo genome editing. Here, we report structures of meningococcal Cas9 homologs in complex with sgRNA, dsDNA, or the AcrIIC3 a...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2019.09.025

    authors： Sun W,Yang J,Cheng Z,Amrani N,Liu C,Wang K,Ibraheim R,Edraki A,Huang X,Wang M,Wang J,Liu L,Sheng G,Yang Y,Lou J,Sontheimer EJ,Wang Y

    更新日期：2019-12-19 00:00:00

  • GPS2 is required for cholesterol efflux by triggering histone demethylation, LXR recruitment, and coregulator assembly at the ABCG1 locus.

    abstract：:Transcriptional coregulators, rather than ligand signals, are suspected to confer context and pathway specificity to nuclear receptor signaling, but the identity of such specifying coregulators and the underlying molecular mechanisms remain largely enigmatic. Here we address this issue in metabolic oxysterol receptor ...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2009.05.006

    authors： Jakobsson T,Venteclef N,Toresson G,Damdimopoulos AE,Ehrlund A,Lou X,Sanyal S,Steffensen KR,Gustafsson JA,Treuter E

    更新日期：2009-05-14 00:00:00

  • Cyclin-dependent kinase 6 is a chromatin-bound cofactor for NF-κB-dependent gene expression.

    abstract：:Given the intimate link between inflammation and dysregulated cell proliferation in cancer, we investigated cytokine-triggered gene expression in different cell cycle stages. Transcriptome analysis revealed that G1 release through cyclin-dependent kinase 6 (CDK6) and CDK4 primes and cooperates with the cytokine-driven...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2013.12.002

    authors： Handschick K,Beuerlein K,Jurida L,Bartkuhn M,Müller H,Soelch J,Weber A,Dittrich-Breiholz O,Schneider H,Scharfe M,Jarek M,Stellzig J,Schmitz ML,Kracht M

    更新日期：2014-01-23 00:00:00

  • An argonaute protein directs nuclear Xrn2 function.

    abstract：:Argonaute proteins are the mediators of small RNA-guided gene silencing pathways. In this issue, Couvillion and coworkers (Couvillion et al., 2012) found an unexpected function for a Tetrahymena Argonaute protein: It forms a complex with tRNA fragments and is required for nuclear Xrn2 localization and function. ...

    journal_title：Molecular cell

    pub_type： 评论,杂志文章

    doi：10.1016/j.molcel.2012.11.015

    authors： Hasler D,Meister G

    更新日期：2012-11-30 00:00:00

  • Crystal structure of the human nuclear cap binding complex.

    abstract：:The heterodimeric nuclear cap binding complex (CBC) binds to 5'-capped polymerase II transcripts. It enhances the efficiency of several mRNA maturation steps and is essential for U snRNA nuclear export in multicellular eukaryotes. The 2A crystal structure of human CBC shows that the large subunit, CBP80, comprises thr...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/s1097-2765(01)00299-4

    authors： Mazza C,Ohno M,Segref A,Mattaj IW,Cusack S

    更新日期：2001-08-01 00:00:00

  • Cooperative activation of PI3K by Ras and Rho family small GTPases.

    abstract：:Phosphoinositide 3-kinases (PI3Ks) and Ras and Rho family small GTPases are key regulators of cell polarization, motility, and chemotaxis. They influence each other's activities by direct and indirect feedback processes that are only partially understood. Here, we show that 21 small GTPase homologs activate PI3K. Usin...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2012.05.007

    authors： Yang HW,Shin MG,Lee S,Kim JR,Park WS,Cho KH,Meyer T,Heo WD

    更新日期：2012-07-27 00:00:00

  • Hos1 deacetylates Smc3 to close the cohesin acetylation cycle.

    abstract：:Cohesion between sister chromatids is mediated by the chromosomal cohesin complex. In budding yeast, cohesin is loaded onto chromosomes during the G1 phase of the cell cycle. During S phase, the replication fork-associated acetyltransferase Eco1 acetylates the cohesin subunit Smc3 to promote the establishment of siste...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2010.08.009

    authors： Borges V,Lehane C,Lopez-Serra L,Flynn H,Skehel M,Rolef Ben-Shahar T,Uhlmann F

    更新日期：2010-09-10 00:00:00

  • SON controls cell-cycle progression by coordinated regulation of RNA splicing.

    abstract：:It has been suspected that cell-cycle progression might be functionally coupled with RNA processing. However, little is known about the role of the precise splicing control in cell-cycle progression. Here, we report that SON, a large Ser/Arg (SR)-related protein, is a splicing cofactor contributing to efficient splici...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2011.03.014

    authors： Ahn EY,DeKelver RC,Lo MC,Nguyen TA,Matsuura S,Boyapati A,Pandit S,Fu XD,Zhang DE

    更新日期：2011-04-22 00:00:00

  • The dynamics of genome-wide DNA methylation reprogramming in mouse primordial germ cells.

    abstract：:Genome-wide DNA methylation reprogramming occurs in mouse primordial germ cells (PGCs) and preimplantation embryos, but the precise dynamics and biological outcomes are largely unknown. We have carried out whole-genome bisulfite sequencing (BS-Seq) and RNA-Seq across key stages from E6.5 epiblast to E16.5 PGCs. Global...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2012.11.001

    authors： Seisenberger S,Andrews S,Krueger F,Arand J,Walter J,Santos F,Popp C,Thienpont B,Dean W,Reik W

    更新日期：2012-12-28 00:00:00