Abstract:
:To determine whether opioid receptors (ORs) are involved in the delayed cardioprotection of ischemic preconditioning (IP), the effect of severe metabolic inhibition (MI) with a glucose-free buffer that contained sodium cyanide and 2-deoxy-D-glucose on the viability of isolated rat ventricular myocytes was first determined 20 hours after preconditioning with a sublethal metabolic inhibition (MIP) with a glucose-free buffer that contained 2-deoxy-D-glucose and lactate for 30 minutes in the presence of OR antagonists. With the use of trypan blue exclusion as an index of cell viability, severe MI killed >60% of the cells and the value increased significantly after MIP. In the presence of 5x10(-6) mol/L nor-binaltorphimine (nor-BNI), a selective kappa-OR antagonist, but not 5x10(-6) mol/L CTOP, a selective mu-OR antagonist, or 5x10(-6) mol/L naltrindole, a selective delta-OR antagonist, the cardioprotection of MIP was significantly attenuated. To verify the role of kappa-OR, we studied the effects of severe MI after pretreatment with the kappa-OR agonist U50,488H (UP) for 30 minutes. U50,488H at 3x10(-6) to 1x10(-4) mol/L increased cell viability concentration-dependently with an EC50 of 3.311x10(-6) mol/L. In the presence of 5x10(-6) nor-BNI, the cardioprotection of UP (3x10(-5) mol/L) was blocked. A time course study showed that UP-induced cardioprotection occurred in 2 windows: the first occurred approximately 1 hour later and the other occurred 16 to 20 hours later. Additional studies on cell contraction and intracellular Ca2+ ([Ca2+]i) revealed that both UP and MIP attenuated the inhibitory effects of severe MI on contractility and electrically induced [Ca2+]i transient in single ventricular myocytes. On blockade of protein kinase C, the delayed cardioprotections of UP and MIP were significantly attenuated. In conclusion, the results of the present study have provided evidence that kappa-OR mediates the cardioprotection of MIP, which may involve protein kinase C and [Ca2+]i.
journal_name
Circ Resjournal_title
Circulation researchauthors
Wu S,Li HY,Wong TMdoi
10.1161/01.res.84.12.1388subject
Has Abstractpub_date
1999-06-25 00:00:00pages
1388-95issue
12eissn
0009-7330issn
1524-4571journal_volume
84pub_type
杂志文章abstract::The relationship of P-wave polarity and morphology in leads II, III, and aVF to the sequence of atrial activation was studied in the canine heart when the atria were paced from the region of the sinus node or the posterior-inferior left atrium and when retrograde activation of the atria occurred with right ventricular...
journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.res.37.2.156
更新日期:1975-08-01 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.RES.0000173849.68636.1e
更新日期:2005-07-08 00:00:00
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pub_type: 杂志文章
doi:10.1161/01.res.40.2.143
更新日期:1977-02-01 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章,评审
doi:10.1161/RES.0000000000000110
更新日期:2016-08-19 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章,评审
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pub_type: 杂志文章
doi:10.1161/01.res.46.1.77
更新日期:1980-01-01 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章,评审
doi:10.1161/CIRCRESAHA.113.300268
更新日期:2013-08-30 00:00:00
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pub_type: 杂志文章,评审
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更新日期:2009-03-27 00:00:00
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pub_type: 杂志文章
doi:10.1161/01.res.71.3.526
更新日期:1992-09-01 00:00:00
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pub_type: 杂志文章
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更新日期:1977-10-01 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.res.0000041418.51906.57
更新日期:2002-11-15 00:00:00
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pub_type: 杂志文章
doi:10.1161/01.res.77.2.239
更新日期:1995-08-01 00:00:00
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pub_type: 杂志文章
doi:10.1161/01.res.39.2.245
更新日期:1976-08-01 00:00:00
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pub_type: 杂志文章
doi:10.1161/01.res.69.3.881
更新日期:1991-09-01 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.res.40.4.348
更新日期:1977-04-01 00:00:00
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pub_type: 杂志文章
doi:10.1161/01.RES.0000081594.21764.44
更新日期:2003-07-25 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/CIRCRESAHA.115.307298
更新日期:2016-02-05 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
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更新日期:1991-10-01 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/CIRCRESAHA.118.314416
更新日期:2019-09-13 00:00:00
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更新日期:1997-02-01 00:00:00
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pub_type: 杂志文章
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更新日期:1985-10-01 00:00:00
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更新日期:1997-06-01 00:00:00
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pub_type: 杂志文章
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更新日期:1981-05-01 00:00:00
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更新日期:1992-08-01 00:00:00
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pub_type: 杂志文章
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更新日期:1993-02-01 00:00:00
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pub_type: 杂志文章
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更新日期:2008-07-03 00:00:00
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pub_type: 杂志文章
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更新日期:1998-07-27 00:00:00