Abstract:
:Recently, there have been significant advances toward the development of culture conditions that promote proliferation of primary rodent hepatocytes. There are two major methods for the multiplication of hepatocytes in vitro: one is the use of nicotinamide, the other is the use of a nutrient-rich medium. In the medium containing a high concentration of nicotinamide and a growth factor, primary hepatocytes can proliferate well. In this culture condition small mononucleate cells, which are named small hepatocytes, appear and form colonies. Small hepatocytes have a high potential to proliferate while maintaining hepatic characteristics, and can differentiate into mature ones. On the other hand, combining the nutrient-rich medium with 2% DMSO, the proliferated hepatocytes can recover the hepatic differentiated functions and maintain them for a long time. In this review I describe the culture conditions for the proliferation and differentiation of primary hepatocytes and discuss the small hepatocytes, especially their roles in liver growth.
journal_name
Int J Exp Patholjournal_title
International journal of experimental pathologyauthors
Mitaka Tdoi
10.1046/j.1365-2613.1998.00083.xsubject
Has Abstractpub_date
1998-12-01 00:00:00pages
393-409issue
6eissn
0959-9673issn
1365-2613journal_volume
79pub_type
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