Beta-catenin mutations are more frequent in small colorectal adenomas than in larger adenomas and invasive carcinomas.

Abstract:

:Loss of serine or threonine phosphorylation sites from exon 3 of beta-catenin has been identified in approximately half of colorectal tumors which lack adenomatous polyposis coli (APC) mutations, but the overall contribution of beta-catenin mutations to sporadic colorectal tumorigenesis is unclear. We therefore used PCR to amplify and sequence exon 3 of beta-catenin from 202 sporadic colorectal tumors. Exon 3 beta-catenin mutations were identified in 6 of 48 small (< 1 cm) adenomas, 2 of 82 large (> or =1 cm) adenomas, and 1 of 72 invasive carcinomas. Eight of the nine mutations, including all of those in the small adenomas and the invasive cancer, involved loss of serine or threonine phosphorylation sites. The percentage of beta-catenin mutations in small adenomas (12.5%) was significantly greater than that in large adenomas (2.4%) and invasive cancers (1.4%; P = 0.05 and P = 0.02, respectively). We conclude that mutation of beta-catenin can be an early, perhaps initiating, event in colorectal tumorigenesis. Small adenomas with beta-catenin mutations do not appear to be as likely to progress to larger adenomas and invasive carcinomas as other adenomas, however, with the result that beta-catenin mutations are only rarely seen in invasive cancers. This suggests that APC and beta-catenin mutations are not functionally equivalent, and that the APC gene may have other tumor suppressor functions besides the degradation of beta-catenin.

journal_name

Cancer Res

journal_title

Cancer research

authors

Samowitz WS,Powers MD,Spirio LN,Nollet F,van Roy F,Slattery ML

subject

Has Abstract

pub_date

1999-04-01 00:00:00

pages

1442-4

issue

7

eissn

0008-5472

issn

1538-7445

journal_volume

59

pub_type

杂志文章
  • Pim kinase inhibitors sensitize prostate cancer cells to apoptosis triggered by Bcl-2 family inhibitor ABT-737.

    abstract::Pim serine/threonine kinases contribute to prostate tumorigenesis and therapeutic resistance, yet Pim kinase inhibitors seem to have only limited effects on prostate cancer cell survival. Because overexpression of Bcl-2 family members are implicated in chemotherapeutic resistance in prostate cancer, we investigated th...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:10.1158/0008-5472.CAN-11-3240

    authors: Song JH,Kraft AS

    更新日期:2012-01-01 00:00:00

  • SPARC/osteonectin induces matrix metalloproteinase 2 activation in human breast cancer cell lines.

    abstract::Activation of the matrix metalloproteinase 2 (MMP-2) has been shown to play a major role in the proteolysis of extracellular matrix (ECM) associated with tumor invasion. Although the precise mechanism of this activation remains elusive, levels of the membrane type 1-MMP (MT1-MMP) at the cell surface and of the tissue ...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Gilles C,Bassuk JA,Pulyaeva H,Sage EH,Foidart JM,Thompson EW

    更新日期:1998-12-01 00:00:00

  • Cytokines prevent dexamethasone-induced apoptosis via the activation of mitogen-activated protein kinase and phosphatidylinositol 3-kinase pathways in a new multiple myeloma cell line.

    abstract::A new human myeloma cell line, OPM-6, was established from the peripheral blood of a patient with advanced IgG-kappa plasma cell leukemia. Cytogenetic and phenotypic analysis confirmed that the cells were derived from the patient's leukemic cells. Insulin-like growth factor-1 (IGF-1) acts as an autocrine growth factor...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Ogawa M,Nishiura T,Oritani K,Yoshida H,Yoshimura M,Okajima Y,Ishikawa J,Hashimoto K,Matsumura I,Tomiyama Y,Matsuzawa Y

    更新日期:2000-08-01 00:00:00

  • Lower heat shock factor activation and binding and faster rate of HSP-70A messenger RNA turnover in heat sensitive human leukemias.

    abstract::Normal bone marrow progenitors and some leukemic cells develop only a limited amount of thermotolerance. Further, once developed, thermotolerance decays at a faster rate than that normally observed in cells of nonhemopoietic origin. Thermotolerance induction and maintenance correlates with reduced levels of expression...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Mivechi NF,Ouyang H,Hahn GM

    更新日期:1992-12-15 00:00:00

  • Bcr interacts with components of the endosomal sorting complex required for transport-I and is required for epidermal growth factor receptor turnover.

    abstract::Virtually all patients with chronic myelogenous leukemia (CML) express an aberrant protein (p210 Bcr-Abl) that contains NH2-terminal sequences from Bcr fused to COOH-terminal sequences from Abl. In a yeast two-hybrid screen, we have identified TSG101 as a binding partner for Bcr. Because TSG101 is a subunit of the mam...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:10.1158/0008-5472.CAN-06-0536

    authors: Olabisi OO,Mahon GM,Kostenko EV,Liu Z,Ozer HL,Whitehead IP

    更新日期:2006-06-15 00:00:00

  • Selective suppression of N-acetylglucosaminyltransferase III activity in a human hepatoblastoma cell line transfected with hepatitis B virus.

    abstract::UDP-N-acetylglucosamine: beta-D-mannoside beta-1,4-N-acetylglucosaminyl-transferase III (GnT-III) is a key enzyme in the branching of asparagine-linked oligosaccharides, which are present in surface membrane proteins of various tissues and in secretory glycoproteins. The activity of GnT-III was assayed in 2 human hepa...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Miyoshi E,Nishikawa A,Ihara Y,Hayashi N,Fusamoto H,Kamada T,Taniguchi N

    更新日期:1994-04-01 00:00:00

  • Biological monitoring screening of patients provided antineoplastic drugs including adriamycin, cyclophosphamide, 5-fluorouracil, methotrexate, and vincristine.

    abstract::The aim of the present study was to establish screening biomarkers of exposure to antineoplastic drugs administered to 11 patients undergoing cancer chemotherapy. Among the anticancer drugs administered were cyclophosphamide (all), Adriamycin (5 of 11), methotrexate (3 of 11), 5-fluorouracil (4 of 11), vincristine (3 ...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Newman MA,Hee SQ,Schoeny R,Lowry L

    更新日期:1990-06-01 00:00:00

  • EpCAM Signaling Promotes Tumor Progression and Protein Stability of PD-L1 through the EGFR Pathway.

    abstract::Although epithelial cell adhesion molecule (EpCAM) has previously been shown to promote tumor progression, the underlying mechanisms remain largely unknown. Here, we report that the EGF-like domain I within the extracellular domain of EpCAM (EpEX) binds EGFR, activating both AKT and MAPK signaling to inhibit forkhead ...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:10.1158/0008-5472.CAN-20-1264

    authors: Chen HN,Liang KH,Lai JK,Lan CH,Liao MY,Hung SH,Chuang YT,Chen KC,Tsuei WW,Wu HC

    更新日期:2020-11-15 00:00:00

  • Prostate cancer associated with p53 and Rb deficiency arises from the stem/progenitor cell-enriched proximal region of prostatic ducts.

    abstract::Recently, we have shown that prostate epithelium-specific deficiency for p53 and Rb tumor suppressors leads to metastatic cancer, exhibiting features of both luminal and neuroendocrine differentiation. Using stage-by-stage evaluation of carcinogenesis in this model, we report that all malignant neoplasms arise from th...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:10.1158/0008-5472.CAN-07-0768

    authors: Zhou Z,Flesken-Nikitin A,Nikitin AY

    更新日期:2007-06-15 00:00:00

  • Regulation of glucocorticoid receptor protein and mRNA levels.

    abstract::The level of steroid receptors in target cells affects the responsiveness of the cell to the hormone. In mouse AtT-20 cells, it has been shown that chronic glucocorticoid treatment causes a down-regulation of glucocorticoid receptor (GR) levels (F. Svec and M. Rudis, J. Biol. Chem., 256:5984-5987, 1981). The current s...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Vedeckis WV,Ali M,Allen HR

    更新日期:1989-04-15 00:00:00

  • Effect of progressive neoplastic growth on the decarboxylation of DL-[1-14C]ornithine by lymphocytes from C3H/He tumor hosts.

    abstract::The reactivity of normal and tumor host lymphocytes incubated with normal serum or with serum or malignant ascites fluid from tumor hosts was measured by the ability of the lymphocytes to synthesize ornithine decarboxylase after phytohemagglutinin stimulation. Each of three tumors tested (a solid fibrosarcoma, an asci...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Vaage J,Agarwal S,Reinertsen JL

    更新日期:1978-08-01 00:00:00

  • Neuronal guidance protein Netrin-1 induces differentiation in human embryonal carcinoma cells.

    abstract::Pluripotent cells within embryonal carcinoma (EC) can differentiate in vivo or in vitro on treatment with specific agents. Differentiating EC cells express lower levels of stem cell-related genes, such as Cripto-1. We show that migration of human EC cells (NTERA/2 and NCCIT) can be reduced following treatment with the...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:10.1158/0008-5472.CAN-08-2985

    authors: Mancino M,Esposito C,Watanabe K,Nagaoka T,Gonzales M,Bianco C,Normanno N,Salomon DS,Strizzi L

    更新日期:2009-03-01 00:00:00

  • SUMO-1 modification of the Wilms' tumor suppressor WT1.

    abstract::SUMO-1 conjugation modulates numerous cellular functions, including the subnuclear localization of its target proteins. The WT1 tumor suppressor encodes a four-zinc finger protein with distinct splicing isoforms. WT1(-KTS), encoding uninterrupted zinc fingers, functions as a transcription factor and has a diffusely nu...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:10.1158/0008-5472.CAN-04-1502

    authors: Smolen GA,Vassileva MT,Wells J,Matunis MJ,Haber DA

    更新日期:2004-11-01 00:00:00

  • Enhanced radiation sensitivity in HPV-positive head and neck cancer.

    abstract::Patients with human papillomavirus (HPV+)-associated head and neck cancer (HNC) show significantly improved survival outcome compared with those with HPV-negative (HPV-) tumors. Published data examining this difference offers conflicting results to date. We systematically investigated the radiation sensitivity of all ...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:10.1158/0008-5472.CAN-13-0587

    authors: Kimple RJ,Smith MA,Blitzer GC,Torres AD,Martin JA,Yang RZ,Peet CR,Lorenz LD,Nickel KP,Klingelhutz AJ,Lambert PF,Harari PM

    更新日期:2013-08-01 00:00:00

  • Identification of breast tumor mutations in BRCA1 that abolish its function in homologous DNA recombination.

    abstract::Effects of breast cancer-associated gene 1 (BRCA1) missense mutations on the function of BRCA1 protein in DNA recombination have been little studied. In this report, we adapted a homology-directed recombination (HDR) assay to analyze the effects of BRCA1 mutations on this function. Using a HeLa-derived cell line with ...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:10.1158/0008-5472.CAN-09-2850

    authors: Ransburgh DJ,Chiba N,Ishioka C,Toland AE,Parvin JD

    更新日期:2010-02-01 00:00:00

  • Osteoclasts direct bystander killing of bone cancer.

    abstract::Primary and metastatic bone cancers are difficult to eradicate and novel approaches are needed to improve treatment and extend life. As bone cancer grows, osteoclasts, the principal bone-resorbing cells of the body, are recruited to and activated at sites of cancer. In this investigation, we determined if osteoclast l...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:10.1158/0008-5472.CAN-06-1295

    authors: Ramnaraine ML,Mathews WE,Donohue JM,Lynch CM,Goblirsch MJ,Clohisy DR

    更新日期:2006-11-15 00:00:00

  • Adaptive Upregulation of EGFR Limits Attenuation of Tumor Growth by Neutralizing IL6 Antibodies, with Implications for Combined Therapy in Ovarian Cancer.

    abstract::Excess production of the proinflammatory IL6 has both local and systemic tumor-promoting activity in many cancers, including ovarian cancer. However, treatment of advanced ovarian cancer patients with a neutralizing IL6 antibody yielded little efficacy in a previous phase II clinical trial. Here, we report results tha...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:10.1158/0008-5472.CAN-14-1801

    authors: Milagre CS,Gopinathan G,Everitt G,Thompson RG,Kulbe H,Zhong H,Hollingsworth RE,Grose R,Bowtell DD,Hochhauser D,Balkwill FR

    更新日期:2015-04-01 00:00:00

  • HSP27 phosphorylation-mediated resistance against actin fragmentation and cell death induced by oxidative stress.

    abstract::Phosphorylation of heat shock protein 27 (HSP27) has been suggested to play an important role in the regulation of F-actin dynamics in response to growth factors and stress. Because the microfilament network is one of the earliest targets of oxidative stress and because phosphorylation of HSP27 is strongly induced by ...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Huot J,Houle F,Spitz DR,Landry J

    更新日期:1996-01-15 00:00:00

  • Reduced toxicity and enhanced antitumor effects in mice of the ionophoric drug valinomycin when incorporated in liposomes.

    abstract::Valinomycin (NSC 122023) is a cyclic depsipeptide antibiotic with potassium selective ionophoric activity. This drug has been reported to display antitumor effects but its utilization has been limited by its extreme toxicity. Here we report that the incorporation of valinomycin into multilamellar liposomes composed of...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Daoud SS,Juliano RL

    更新日期:1986-11-01 00:00:00

  • Induction of release of cytotoxin from murine bone marrow cells by an animal lectin.

    abstract::An animal lectin purified from loach (Misgurnus anguillicaudatus) eggs induced release of cytotoxin from fresh bone marrow cells from mice, although the other lectins tested, wheat germ agglutinin, concanavalin A, and phytohemagglutinin did not. The cytotoxin released from bone marrow cells was a heat-labile protein w...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Okutomi T,Nakajima Y,Sakakibara F,Kawauchi H,Yamazaki M

    更新日期:1987-01-01 00:00:00

  • Cell division cycle 25B phosphatase is essential for benzo(a)pyrene-7,8-Diol-9,10-epoxide induced neoplastic transformation.

    abstract::Cell division cycle 25B (Cdc25B) phosphatase controls entry into mitosis and regulates recovery from G2-M checkpoint-induced arrest. In the present study, we show that exposure of diploid mouse embryonic fibroblasts (MEF) to the ultimate carcinogen anti-benzo(a)pyrene (BP)-7,8-diol-9,10-epoxide (anti-BPDE) resulted in...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:10.1158/0008-5472.CAN-07-0025

    authors: Srivastava SK,Bansal P,Oguri T,Lazo JS,Singh SV

    更新日期:2007-10-01 00:00:00

  • Oncogenic transformation of C3H/10T1/2 clone 8 mouse embryo cells by halogenated pyrimidine nucleosides.

    abstract::Oncogenic transformation has been induced in vitro in the C3H/10T1/2 clone 8 line of mouse cells by exposure to 5-fluoro-2'-deoxyuridine (FUdR) or 5-fluorouracil. This transformation is both dose and time dependent and can be markedly decreased by simultaneous exposure of the cells to thymidine. The transformation ind...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Jones PA,Benedict WF,Baker MS,Mondal S,Rapp U,Heidelberger C

    更新日期:1976-01-01 00:00:00

  • A monoclonal antibody, CSTO-1, against a stomach adenocarcinoma-associated antigen.

    abstract::A monoclonal antibody, CSTO-1, has been produced against a stomach adenocarcinoma-associated antigen. The antibody is cytotoxic to stomach, colon, and lung adenocarcinoma lines but is completely noncytotoxic to normal blood elements and leukemic cell lines. The monoclonal antibody reacts with tumor cell membranes in e...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Togashi H,Terasaki PI,Chia D,Kukes G,Mugishima H,Kasai K,Suyama N,Fukushima K,Hirota M

    更新日期:1984-09-01 00:00:00

  • In vivo flow cytometry: a new method for enumerating circulating cancer cells.

    abstract::The fate of circulating tumor cells is an important determinant of their ability to form distant metastasis. Here, we demonstrate the use of in vivo flow cytometry as a powerful new method for detecting quantitatively circulating cancer cells. We specifically examine the circulation kinetics of two prostate cancer cel...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:10.1158/0008-5472.CAN-04-1058

    authors: Georgakoudi I,Solban N,Novak J,Rice WL,Wei X,Hasan T,Lin CP

    更新日期:2004-08-01 00:00:00

  • Allelic loss on chromosome 3p21.3 and promoter hypermethylation of semaphorin 3B in non-small cell lung cancer.

    abstract::The aim of this study was to evaluate the promoter methylation status and loss of heterozygosity (LOH) of the SEMA3B in non-small cell lung cancers (NSCLCs). We analyzed the methylation status of semaphorin 3B (SEMA3B) promoter and LOH at 3p21.3 in eight NSCLC cell lines and 27 primary tumors. Hypermethylation of SEMA...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Kuroki T,Trapasso F,Yendamuri S,Matsuyama A,Alder H,Williams NN,Kaiser LR,Croce CM

    更新日期:2003-06-15 00:00:00

  • Isolation of preneoplastic rat liver cells by centrifugal elutriation and binding to asialofetuin.

    abstract::Putative preneoplastic hepatocytes were isolated from male Fischer 344 rats treated with a single dose of diethylnitrosamine, 2-acetylaminofluorene feeding, and partial hepatectomy (Solt-Farber model). The isolation procedure involved, after collagenase dispersion of the liver, separation of the hepatocytes into small...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Evarts RP,Marsden E,Hanna P,Wirth PJ,Thorgeirsson SS

    更新日期:1984-12-01 00:00:00

  • Dominant-negative activator protein 1 (TAM67) targets cyclooxygenase-2 and osteopontin under conditions in which it specifically inhibits tumorigenesis.

    abstract::Activation of activator protein 1 (AP-1) and nuclear factor kappaB (NFkappaB)-dependent transcription is required for tumor promotion in cell culture models and transgenic mice. Dominant-negative c-Jun (TAM67) blocks AP-1 activation by dimerizing with Jun or Fos family proteins and blocks NFkappaB activation by intera...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:10.1158/0008-5472.CAN-06-0522

    authors: Matthews CP,Birkholz AM,Baker AR,Perella CM,Beck GR Jr,Young MR,Colburn NH

    更新日期:2007-03-15 00:00:00

  • Monitoring immunotherapy with Bacillus Calmette-Guérin by antibody titer.

    abstract::Antibody titer to Bacillus Calmette-Guérin (BCG) was determined by complement fixation in 111 normal volunteers and 83 melanoma patients. In 43 of these melanoma patients, sequential determination of antibody titer was made and correlated with clinical course. Low titers of antibody to BCG were found frequently in nor...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Wile AG,Sparks FC,Morton DL

    更新日期:1977-07-01 00:00:00

  • Antitumor activity of intraperitoneal immunotoxins in a nude mouse model of human malignant mesothelioma.

    abstract::Immunotoxins directed against human transferrin receptor have been evaluated in a nude mouse model of human malignant mesothelioma. Immunotoxins were constructed by linking ricin A chain to murine monoclonal antibodies reactive with the human transferrin receptor. A chain was obtained either by isolation from the pare...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Griffin TW,Richardson C,Houston LL,LePage D,Bogden A,Raso V

    更新日期:1987-08-15 00:00:00

  • Effects of physiological oxygen concentration on human tumor colony growth in soft agar.

    abstract::Cloning efficiencies of 67 fresh human tumor specimens, consisting of 29 ovarian, 10 lung, and 7 each of colon, breast, mesenchymal, and miscellaneous tumors, from 58 patients were studied using the technique of Hamburger and Salmon to evaluate the effect of incubation in a 5% rather than 20% oxygen environment. Under...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Sridhar KS,Plasse TF,Holland JF,Shapiro M,Ohnuma T

    更新日期:1983-10-01 00:00:00