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Synaptic targeting of the postsynaptic density protein PSD-95 mediated by lipid and protein motifs.

Abstract：

:During synaptic development, proteins aggregate at specialized pre- and postsynaptic structures. Mechanisms that mediate protein clustering at these sites remain unknown. To investigate this process, we analyzed synaptic targeting of a postsynaptic density protein, PSD-95, by expressing green fluorescent protein- (GFP-) tagged PSD-95 in cultured hippocampal neurons. We find that postsynaptic clustering relies on three elements of PSD-95: N-terminal palmitoylation, the first two PDZ domains, and a C-terminal targeting motif. In contrast, disruptions of PDZ3, SH3, or guanylate kinase (GK) domains do not affect synaptic targeting. Palmitoylation is sufficient to target the diffusely expressed SAP-97 to synapses, and palmitoylation cannot be replaced with alternative membrane association motifs, suggesting that a specialized synaptic lipid environment mediates postsynaptic clustering. The requirements for PDZ domains and a C-terminal domain of PSD-95 indicate that protein-protein interactions cooperate with lipid interactions in synaptic targeting.

journal_name

Neuron

journal_title

Neuron

authors

Craven SE,El-Husseini AE,Bredt DS

doi

10.1016/s0896-6273(00)80705-9

subject

Has Abstract

pub_date

1999-03-01 00:00:00

pages

497-509

issue

3

eissn

0896-6273

issn

1097-4199

pii

S0896-6273(00)80705-9

journal_volume

22

pub_type

杂志文章

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