Abstract:
:In order to clarify the function of hepatocyte growth factor (HGF) in vivo, we have developed transgenic mice expressing HGF in the liver. The bromodeoxyuridine labelling indices in livers from HGF transgenic mice were doubled, compared to those from wild type mice. Livers of HGF transgenic mice expressed high levels of c-myc, which was the consequence of increased transcription rates through the c-myc promoter. After 70% partial hepatectomy, the livers of HGF transgenic mice recovered in half the time needed for their normal siblings. Since we found that HGF inhibits growth of hepatocellular carcinoma (HCC) cells in vitro, we have made two kinds of double transgenic mice: HGF/TGF alpha and HGF/c-myc mice. The double transgenic mice expressing both HGF and TGF alpha had lower tumour yields, compared to TGF alpha transgenic mice. The HGF/c-myc double transgenic mice had a lower incidence of hepatocellular adenoma (HCA) and HCC in comparison with c-myc transgenic mice. In HGF/c-myc mice, there were more apoptotic cells and less mitotic cells than c-myc transgenic mice. These data indicate that HGF inhibits growth and occurrence of HCC in vivo. We also found that HGF protects liver from D-galactosamine (D-GalN)-induced injury. Hepatic prostaglandin E 2 (PGE2) contents in HGF transgenic mice were much higher than those in wild type mice, and were associated with hepatic HGF contents. An anti-HGF antibody inhibits production of PGE2 in liver after D-GalN administration. These data suggest that HGF protects liver from D-GalN-induced injury through increased liver PGE2 production. The data obtained from HGF transgenic mice suggests the possibility that HGF could be applicable for therapy of human liver diseases in the future.
journal_name
Int J Exp Patholjournal_title
International journal of experimental pathologyauthors
Shiota G,Kawasaki Hdoi
10.1046/j.1365-2613.1998.730403.xsubject
Has Abstractpub_date
1998-10-01 00:00:00pages
267-77issue
5eissn
0959-9673issn
1365-2613journal_volume
79pub_type
杂志文章,评审abstract::Heparan sulphate (HS) sits at the interface of the cell and the extracellular matrix. It is a member of the glycosaminoglycan family of anionic polysaccharides with unique structural features designed for protein interaction and regulation. Its client proteins include soluble effectors (e.g. growth factors, morphogens...
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journal_title:International journal of experimental pathology
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journal_title:International journal of experimental pathology
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journal_title:International journal of experimental pathology
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journal_title:International journal of experimental pathology
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journal_title:International journal of experimental pathology
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journal_title:International journal of experimental pathology
pub_type: 杂志文章
doi:
更新日期:1990-10-01 00:00:00
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journal_title:International journal of experimental pathology
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journal_title:International journal of experimental pathology
pub_type: 杂志文章
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journal_title:International journal of experimental pathology
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journal_title:International journal of experimental pathology
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journal_title:International journal of experimental pathology
pub_type: 杂志文章
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journal_title:International journal of experimental pathology
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journal_title:International journal of experimental pathology
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journal_title:International journal of experimental pathology
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更新日期:1991-12-01 00:00:00
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journal_title:International journal of experimental pathology
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更新日期:1993-06-01 00:00:00
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journal_title:International journal of experimental pathology
pub_type: 杂志文章
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更新日期:1992-04-01 00:00:00
abstract::Conventional therapies for low back pain (LBP) are purely symptomatic and do not target the cause of LBP, which in approximately 40% of cases is caused by degeneration of the intervertebral disc (DIVD). Targeting therapies to inhibit the process of degeneration would be a potentially valuable treatment for LBP. There ...
journal_title:International journal of experimental pathology
pub_type: 杂志文章
doi:10.1111/j.0959-9673.2006.00449.x
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abstract::This study aimed to characterize matrix metalloproteinases (MMPs) and their tissue inhibitors (TIMPs) in relation to changes in left ventricle (LV) geometry and function in a porcine model with streptozotocin (STZ)-induced diabetes. In 15 Chinese Guizhou minipigs with STZ-induced diabetes (diabetic group) and 15 age-m...
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