Abstract：
:Using a specific rat monoclonal anti-idiotypic antibody we have examined the subcellular distribution of -opioid receptors in various neuronal subtypes of the rat spinal cord. The immunofluorescence was detected with a confocal microscope and in some cases serial images were processed for a three-dimensional (3-D) reconstruction of the neurons. Immunolabelling was found to be distributed throughout the spinal cord grey matter specially in the most superficial layers of the dorsal horn, around the central canal and in the region of motoneurons of the ventral horn. The 3-D reconstruction made on large neurons of lamina IX in the ventral horn and on neurons of lamina X around the central canal allowed the visualization of 5 -opioid receptors in the cytoplasm of the soma and proximal neurites of immunofluorescent neurons. Some immunolabelled receptors were also detected at the level of the plasma membrane of the cell bodies and in the nuclear matrix. Interestingly, a particular arrangement of delta-opioid receptors organized along parallel alignments was observed on the plasma membrane of some neurons. This study emphasizes the potential usefulness of a 3-D reconstruction in the study of the spatial arrangement of cellular components.
journal_name
J Neurosci Methodsjournal_title
Journal of neuroscience methodsauthors
Mailly P,Gastard M,Cupo Adoi
10.1016/s0165-0270(98)00149-6subject
Has Abstractpub_date
1999-02-01 00:00:00pages
17-24issue
1eissn
0165-0270issn
1872-678Xpii
S0165027098001496journal_volume
87pub_type
杂志文章abstract：BACKGROUND:Manual annotation of seizures and interictal-ictal-injury continuum (IIIC) patterns in continuous EEG (cEEG) recorded from critically ill patients is a time-intensive process for clinicians and researchers. In this study, we evaluated the accuracy and efficiency of an automated clustering method to accelerat...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2020.108966
更新日期：2020-10-22 00:00:00
abstract：:We developed a high speed voice coil based whisker stimulator that delivers precise deflections of a single whisker or group of whiskers in a repeatable manner. The device is miniature, quiet, and inexpensive to build. Multiple stimulators fit together for independent stimulation of four or more whiskers. The system c...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2010.12.008
更新日期：2011-03-15 00:00:00
abstract：:This report describes technical adaptations of a traumatic brain injury (TBI) model-largely inspired by Marmarou-in order to monitor microdialysis data and PtiO2 (brain tissue oxygen) before, during and after injury. We particularly focalize on our model requirements which allows us to re-create some drastic pathologi...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2004.04.037
更新日期：2004-12-30 00:00:00
abstract：:We present an effective technique for culture and expansion of Schwann cells (SC) from adult peripheral nerves. Cultures from adult mouse sciatic nerves (one to six nerves per culture) in defined medium showed markedly higher purity and density of SC when the nerve was predegenerated in vivo for 7 days than when it wa...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/s0165-0270(00)00221-1
更新日期：2000-06-30 00:00:00
abstract：:Rapid prototyping (RP) is a useful method for designing and fabricating a wide variety of devices used for neuroscience research. The present study confirms the utility of using fused deposition modeling, a specific form of RP, to produce three devices commonly used for basic science experimentation. The accuracy and ...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2008.03.011
更新日期：2008-07-30 00:00:00
abstract：:Sleep is an innate behavior conserved in all animals and, in vertebrates, is regulated by neuronal circuits in the brain. The conventional techniques of forward and reverse genetics have enabled researchers to investigate the molecular mechanisms that regulate sleep and arousal. However, functional interrogation of ge...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章,评审
doi：10.1016/j.jneumeth.2018.10.016
更新日期：2019-03-15 00:00:00
abstract：BACKGROUND:This study analyses multiple spike trains (MST) data, defines its functional connectivity and subsequently visualises an accurate diagram of connections. This is a challenging problem. For example, it is difficult to distinguish the common input and the direct functional connection of two spike trains. NEW ...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2017.05.016
更新日期：2017-07-15 00:00:00
abstract：BACKGROUND:The brain generates signals in a wide frequency range (∼2840 Hz). Existing magnetoencephalography (MEG) methods typically detect brain activity in a median-frequency range (1-70 Hz). The objective of the present study was to develop a new method to utilize the frequency signatures for source imaging. NEW ME...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2014.10.007
更新日期：2015-01-15 00:00:00
abstract：BACKGROUND:We present our results in the International challenge for automated prediction of MCI from MRI data. We evaluate the performance of MRI-based neuromorphometrics features (nMF) in the classification of Healthy Controls (HC), Mild Cognitive Impairment (MCI), converters MCI (cMCI) and Alzheimer's Disease (AD) p...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2017.11.013
更新日期：2018-05-15 00:00:00
abstract：:Traumatic brain injury (TBI) is a major cause of death and disability for which there is no cure. One of the issues inhibiting clinical trial success is the lack of targeting specific patient populations due to inconsistencies between clinical diagnostic tools and underlying pathophysiology. The development of reliabl...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2011.08.019
更新日期：2011-10-15 00:00:00
abstract：:An opto-electronic system has been developed to measure movements of insect appendages. It is made from a mirror-lens and a linear position-sensitive photodiode. The design of the mirror-lens has been exploited to axially mount a high intensity halogen light source in front of the mirror-lens. The system monitors a re...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/s0165-0270(00)00255-7
更新日期：2000-07-31 00:00:00
abstract：BACKGROUND:Certain inherited progressive neurodegenerative disorders, such as spinocerebellar ataxia (SCA), affect neurons in large areas of the central nervous system (CNS). The selective expression of disease-causing and therapeutic genes in susceptible regions and cell types is critical for the generation of animal ...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2013.12.004
更新日期：2014-02-15 00:00:00
abstract：:In the present study the accumulation of protease resistant prion protein (PrPres) in scrapie-infected neuroblastoma cells (ScN2a cells) was shown to be dependent on culture conditions. The highest levels of PrPres were found in slow growing cells. Further increases in PrPres accumulation were observed in ScN2a cells ...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2004.04.001
更新日期：2004-09-30 00:00:00
abstract：:Experimental evidence points to the importance of mitochondrial transport defects in contributing to major neurodegenerative diseases, such as Parkinson's disease (PD). Studies of mitochondrial transport along single axons are difficult with traditional dissociated culture systems and the fragility of the midbrain dop...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2012.05.021
更新日期：2012-07-30 00:00:00
abstract：BACKGROUND:Mild traumatic brain injury (mTBI) strongly associates with chronic neurodegenerative impairments such as post-traumatic stress disorder (PTSD) and mild cognitive impairment. Early detection of concussive events would significantly enhance the understanding of head injuries and provide better guidance for ur...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2021.109073
更新日期：2021-01-10 00:00:00
abstract：:Microglia are brain cells of considerable interest because of their role in CNS inflammatory responses and strong association with neuritic plaques in Alzheimer's disease (AD). In the present study, immunocytochemistry was combined with unbiased stereology to estimate the mean total number of microglia in dentate gyru...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/s0165-0270(98)00100-9
更新日期：1998-10-01 00:00:00
abstract：:In this article, we describe an efficient method for testing both auditory receptor and auditory nerve function in a single recording session. Auditory receptor function is tested in response to pure tone, tone burst and click acoustic stimuli (i.e. distortion products of otoacoustic emissions, DPOAE; and auditory-evo...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2003.11.003
更新日期：2004-04-30 00:00:00
abstract：BACKGROUND:Fluorescence membrane markers are efficient tools for visualizing the dynamics of membrane recycling processes in living cells. The outer hair cell (OHC) - a bipolar epithelial cell in the cochlea - possesses endocytic activity at both its apical and basal poles. The best visual overview of transcytosis in t...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2017.10.014
更新日期：2018-01-01 00:00:00
abstract：:An isoelectric focusing (IEF) technique using direct peroxidase or peroxidase-antiperoxidase (PAP) overlay staining for detection of cerebrospinal fluid (CSF) oligoclonal IgG is described. The method involves chemical fixation of all proteins in the gel followed by application of peroxidase coupled antiserum as a spec...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(80)90035-7
更新日期：1980-10-01 00:00:00
abstract：:Intrinsic signal imaging (ISI) measures changes in light reflectance from the illuminated cortex (intrinsic signals or IS) attributed to various vascular and metabolic sources that, when using illumination in the 600 nm range, appear to co-localize with neuronal activity. Given the multiple sources contributing to the...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/s0165-0270(00)00180-1
更新日期：2000-04-15 00:00:00
abstract：:The development of high-resolution retinal prostheses fabricated from silicon wafers presents an interesting problem: how to electrically bridge the space between the flat silicon wafer and the curved retinal surface. One potential "bridge" is a microwire glass electrode. In this paper we present our results in evalua...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2004.02.035
更新日期：2004-08-30 00:00:00
abstract：BACKGROUND:Recent advances in genetically engineered calcium and membrane potential indicators provide the potential to estimate the activation dynamics of individual neurons within larger, mesoscale networks (100s-1000+neurons). However, a fully integrated automated workflow for the analysis and visualization of neura...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2015.01.020
更新日期：2015-03-30 00:00:00
abstract：:A procedure is described by which the solid silver chromate precipitate present in Golgi-impregnated neurons is reduced by a photographic developer into metallic silver grains or particles. The remaining silver chromate is subsequently removed, and the fine structure and synaptic relationships of the impregnated neuro...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(83)90023-7
更新日期：1983-04-01 00:00:00
abstract：BACKGROUND:The accuracy of source reconstruction depends on the spatial configuration of the neural sources underlying encephalographic signals, the temporal distance of the source activity, the level and structure of noise in the recordings, and - of course - on the employed inverse method. This plenitude of factors r...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2018.02.016
更新日期：2018-05-01 00:00:00
abstract：:The neocortex is the site of origin of several forms of acquired epilepsy. Here we provide a brief review of experimental models that were recently developed to study neocortical epileptogenesis as well as some major results obtained with these methods. Most of neocortical seizures appear to be nocturnal and it is kno...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章,评审
doi：10.1016/j.jneumeth.2015.08.030
更新日期：2016-02-15 00:00:00
abstract：:A mathematical model is presented by which the regeneration rate and initial delay for peripheral nerve regeneration can be calculated from sensory pinch test data following surgical repair of peripheral nerves. The model is based on the assumption that experimental variations in regeneration distances between animals...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/s0165-0270(05)80004-4
更新日期：1993-06-01 00:00:00
abstract：:Imaging of optical reporters of neural activity across large populations of neurones is a widely used approach for investigating the function of neural circuits in slices and in vivo. Major challenges in analysing such experiments include the automatic identification of neurones and synapses, extraction of dynamic sig...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2010.01.033
更新日期：2010-04-30 00:00:00
abstract：:Zebra finches have been widely used to study neurobiology underlying vocal development. Because only male zebra finches learn song, efficient developmental use of these animals requires early determination of sex at ages that precede maturation of secondary sex characteristics. We have developed a sex determination me...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2007.04.007
更新日期：2007-08-15 00:00:00
abstract：:The present study evaluated high-speed chronoamperometry as a method for measuring the clearance of serotonin (5-HT) from extracellular space in vivo. Male Sprague-Dawley rats were anaesthetized and a Nafion-coated, carbon fiber electrode, attached to a multibarrel pipette, was lowered into the subgranular layer of th...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/s0165-0270(97)00144-1
更新日期：1997-12-30 00:00:00
abstract：:Surface enhanced laser desorption/ionization-time of flight mass spectrometry (SELDI-TOF MS) is an analytical technology for proteomic analysis that combines chromatography and mass spectrometry. At present, this technology is most commonly being exploited for the simultaneous measurement of numerous proteins in serum...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2006.04.025
更新日期：2006-11-15 00:00:00
