Abstract：
:Human ELAV proteins are implicated in cell growth and differentiation via regulation of mRNA expression in the cytoplasm. In human embryonic teratocarcinoma (hNT2) cells transfected with the human neuronal ELAV-like protein, Hel-N1, neurites formed, yet cells were not terminally differentiated. Cells in which neurite formation was associated with Hel-N1 overexpression, also expressed increased levels of endogenous neurofilament M (NF-M) protein, which distributed along the neurites. However, steady-state levels of NF-M mRNA remained similar whether or not hNT2 cells were transfected with Hel-N1. These findings suggest that turnover of NF-M mRNA was not affected by Hel-N1 expression, despite the fact that Hel-N1 can bind to the 3' UTR of NF-M mRNA and was found directly associated with NF-M mRNA in transfected cells. Analysis of the association of NF-M mRNA with the translational apparatus in Hel-N1 transfectants showed nearly complete recruitment to heavy polysomes, indicating that Hel-N1 caused an increase in translational initiation. Our results suggest that the stability and/or translation of ARE-containing mRNAs can be regulated independently by the ELAV protein, Hel-N1, depending upon sequence elements in the 3' UTRs and upon the inherent turnover rates of the mRNAs that are bound to Hel-N1 in vivo.
journal_name
Genes Devjournal_title
Genes & developmentauthors
Antic D,Lu N,Keene JDdoi
10.1101/gad.13.4.449subject
Has Abstractpub_date
1999-02-15 00:00:00pages
449-61issue
4eissn
0890-9369issn
1549-5477journal_volume
13pub_type
杂志文章abstract：:Intron homing in phage T4 occurs in the context of recombination-dependent replication, by virtue of intron-encoded endonucleolytic activity. After the td intron endonuclease I-TevI cleaves the intronless recipient 23 and 25 nucleotides upstream of the intron insertion site, exonucleolytic degradation is required for ...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.10.17.2158
更新日期：1996-09-01 00:00:00
abstract：:Integrin receptors for extracellular matrix are critical for cell motility, but the signals that determine when to stop are not known. Analysis of distal tip cell (DTC) migration during gonadogenesis in Caenorhabditis elegans has revealed the importance of transcription factor vab-3/Pax6 in regulating the alpha integr...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1534807
更新日期：2007-07-01 00:00:00
abstract：:The cytochrome P450 (CYP) gene products such as CYP3A and CYP2B are essential for the metabolism of steroid hormones and xenochemicals including prescription drugs. Nuclear receptor SXR/PXR (steroid and xenobiotic receptor/pregnenolone X receptor) has been shown both biochemically and genetically to activate CYP3A gen...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.846800
更新日期：2000-12-01 00:00:00
abstract：:DNA repair associated with DNA replication is important for the conservation of genomic sequence information, whereas reconstitution of chromatin after replication sustains epigenetic information. We have isolated and characterized mutations in the BRU1 gene of Arabidopsis that suggest a novel link between these under...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.295404
更新日期：2004-04-01 00:00:00
abstract：:Ectopic repression of retinoic acid (RA) receptor target genes by PML/RARA and PLZF/RARA fusion proteins through aberrant recruitment of nuclear corepressor complexes drives cellular transformation and acute promyelocytic leukemia (APL) development. In the case of PML/RARA, this repression can be reversed through trea...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.512009
更新日期：2009-05-15 00:00:00
abstract：:This paper examines ovarian transcription of the divergently oriented yolk protein genes 1 and 2 (yp1 and yp2) of Drosophila melanogaster. We report germ line transformation results demonstrating that yp1 and yp2 are transcribed in the same subpopulations of ovarian follicle cells. Our results show that this expressio...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.4.4.613
更新日期：1990-04-01 00:00:00
abstract：:Many tumors continuously depend on the initiating oncogenes, but whether this extends to their downstream targets is unclear. In this issue of Genes & Development, Sodir and colleagues (pp. 907-916) demonstrate an essential role for endogenous Myc proteins in maintaining the tumor microenvironment, providing an unexpe...
journal_title：Genes & development
pub_type： 评论,杂志文章
doi：10.1101/gad.2053311
更新日期：2011-05-01 00:00:00
abstract：:Hypoxia induces rapid and dramatic changes in cellular metabolism, in part through inhibition of target of rapamycin (TOR) kinase complex 1 (TORC1) activity. Genetic studies have shown the tuberous sclerosis tumor suppressors TSC1/2 and the REDD1 protein to be essential for hypoxia regulation of TORC1 activity in Dros...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1617608
更新日期：2008-01-15 00:00:00
abstract：:DNA replication remains unfinished in many Drosophila polyploid cells, which harbor disproportionately fewer copies of late-replicating chromosomal regions. By analyzing paired-end high-throughput sequence data from polytene larval salivary gland cells, we define 112 underreplicated (UR) euchromatic regions 60-480 kb ...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.245811.114
更新日期：2014-08-15 00:00:00
abstract：:The yeast SAS (Something About Silencing) complex and the histone variant H2A.Z have both previously been linked to an antisilencing function at the subtelomeric regions. SAS is an H4 Lys 16-specific histone acetyltransferase complex. Here we demonstrate that the H4 Lys 16 acetylation by SAS is required for efficient ...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1439206
更新日期：2006-09-15 00:00:00
abstract：:The integration of viral DNA into the host genome is an essential step in the retrovirus life cycle. To understand this process better, we have examined the native state of viral DNA in cells acutely infected by murine leukemia virus (MLV), using both a physical assay for viral DNA and a functional assay for integrati...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.3.4.469
更新日期：1989-04-01 00:00:00
abstract：:Ste5, the prototypic mitogen-activated protein kinase (MAPK) scaffold protein, associates with plasma membrane-tethered Gbetagamma freed upon pheromone receptor occupancy, thereby initiating downstream signaling. We demonstrate that this interaction and membrane binding of an N-terminal amphipathic alpha-helix (PM mot...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1413706
更新日期：2006-07-15 00:00:00
abstract：:A novel 15-kDa protein, RbfA, has been identified by virtue of its ability to act as a high copy suppressor of a previously characterized dominant cold-sensitive mutation (C23U) in 16S rRNA. RbfA is found associated with free 30S ribosomal subunits, but not with 70S ribosomes or polysomes, and is essential for maximal...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.9.5.626
更新日期：1995-03-01 00:00:00
abstract：:The mechanisms that determine the relative positions of floral organs, and thereby their numbers, is a poorly understood aspect of flower development. We isolated a petunia mutant, floozy (fzy), in which the formation of floral organ primordia in the outermost three floral whorls and one of the two bracts at the base ...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.219502
更新日期：2002-03-15 00:00:00
abstract：:GRF2, an abundant yeast protein of Mr approximately 127,000, binds to the GAL upstream activating sequence (UASG) and creates a nucleosome-free region of approximately 230 bp. Purified GRF2 binds to sequences found in many other UASs, in the 35S rRNA enhancer, at centromeres, and at telomeres. Although GRF2 stimulates...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.4.4.503
更新日期：1990-04-01 00:00:00
abstract：:Eukaryotic chromosomes reach their stable rod-shaped appearance in mitosis in a reaction dependent on the evolutionarily conserved condensin complex. Little is known about how and where condensin associates with chromosomes. Here, we analyze condensin binding to budding yeast chromosomes using high-resolution oligonuc...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1675708
更新日期：2008-08-15 00:00:00
abstract：:SNF2 chromatin-remodeling ATPases play an important role in ensuring proper development in higher eukaryotes by controlling accessibility of cis-regulatory DNA regions to transcription factors and to the transcriptional machinery. However, the biological targets controlled by these ATPases are largely unknown. Using g...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1276305
更新日期：2005-04-15 00:00:00
abstract：:The circadian rhythm of pineal melatonin requires the nocturnal increment of serotonin N-acetyltransferase (arylalkylamine N-acetyltransferase [AANAT]) protein. To date, only limited information is available in the critical issue of how AANAT protein expression is up-regulated exclusively at night regardless of its sp...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1519507
更新日期：2007-04-01 00:00:00
abstract：:The Drosophila scratch (scrt) gene is expressed in most or all neuronal precursor cells and encodes a predicted zinc finger transcription factor closely related to the product of the mesoderm determination gene snail (sna). Adult flies homozygous for scrt null alleles have a reduced number of photoreceptors in the eye...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.9.19.2384
更新日期：1995-10-01 00:00:00
abstract：:Appreciable advances into the process of transcript elongation by RNA polymerase II (RNAP II) have identified this stage as a dynamic and highly regulated step of the transcription cycle. Here, we discuss the many factors that regulate the elongation stage of transcription. Our discussion includes the classical elonga...
journal_title：Genes & development
pub_type： 杂志文章,评审
doi：10.1101/gad.1235904
更新日期：2004-10-15 00:00:00
abstract：:One of the first steps in neurogenesis is the diversification of cells along the dorsoventral axis. In Drosophila the central nervous system develops from three longitudinal columns of cells: ventral cells that express the vnd/nk2 homeobox gene, intermediate cells, and dorsal cells that express the msh homeobox gene. ...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.12.22.3591
更新日期：1998-11-15 00:00:00
abstract：:Myxococcus xanthus development requires CsgA, a member of the short-chain alcohol dehydrogenase (SCAD) family of proteins. We show that CsgA and SocA, a protein that can replace CsgA function in vivo, oxidize the 2'-OH glycerol moiety on cardiolipin and phosphatidylglycerol to produce diacylglycerol (DAG), dihydroxyac...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.268482.115
更新日期：2015-09-15 00:00:00
abstract：:Unusually for a eukaryote, genes transcribed by RNA polymerase II (pol II) in Trypanosoma brucei are arranged in polycistronic transcription units. With one exception, no pol II promoter motifs have been identified, and how transcription is initiated remains an enigma. T. brucei has four histone variants: H2AZ, H2BV, ...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1790409
更新日期：2009-05-01 00:00:00
abstract：:The Saccharomyces cerevisiae protein kinase Rim15p was identified previously as a stimulator of meiotic gene expression. Here, we show that loss of Rim15p causes an additional pleiotropic phenotype in cells grown to stationary phase on rich medium; this phenotype includes defects in trehalose and glycogen accumulation...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.12.18.2943
更新日期：1998-09-15 00:00:00
abstract：:The orphan nuclear receptor TLX regulates neural stem cell self-renewal in the adult brain and functions primarily as a transcription repressor through recruitment of Atrophin corepressors, which bind to TLX via a conserved peptide motif termed the Atro box. Here we report crystal structures of the human and insect TL...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.254904.114
更新日期：2015-02-15 00:00:00
abstract：:The regulation of gene transcription is critical for the proper development and growth of an organism. The transcription of protein-coding genes initiates at the RNA polymerase II core promoter, which is a diverse module that can be controlled by many different elements such as the TATA box and downstream core promote...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1698108
更新日期：2008-10-15 00:00:00
abstract：:Lipids play crucial roles in many aspects of glial cell biology, affecting processes ranging from myelin membrane biosynthesis to axo-glial interactions. In order to study the role of lipid metabolism in myelinating glial cells, we specifically deleted in Schwann cells the Lpin1 gene, which encodes the Mg2+-dependent ...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1638008
更新日期：2008-06-15 00:00:00
abstract：:When the orphan nuclear receptors TR2 and TR4, the DNA-binding subunits of the DRED repressor complex, are forcibly expressed in erythroid cells of transgenic mice, embryos exhibit a transient mid-gestational anemia as a consequence of a reduction in the number of primitive erythroid cells. GATA-1 mRNA is specifically...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1593307
更新日期：2007-11-01 00:00:00
abstract：:Activated transcription by RNA polymerase II (Pol II) requires coactivators, one of which is the SRB/mediator. Whereas Srb4, an essential subunit of the SRB/mediator, is broadly required for Pol II transcription in yeast, we have shown that it is dispensable for the transcriptional activation of some genes. Here, we s...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.13.22.2934
更新日期：1999-11-15 00:00:00
abstract：:Nuclear pore complexes (NPCs) emerged as nuclear transport channels in eukaryotic cells ∼1.5 billion years ago. While the primary role of NPCs is to regulate nucleo-cytoplasmic transport, recent research suggests that certain NPC proteins have additionally acquired the role of affecting gene expression at the nuclear ...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.280941.116
更新日期：2016-05-15 00:00:00
