CD34+-selected peripheral blood progenitor cell transplantation in patients with multiple myeloma: tumour cell contamination and outcome.

Abstract:

:Thirty-six patients with multiple myeloma (23 PR1, nine PR2, four stable disease) were entered into a pilot study evaluating the use of CD34+-selected peripheral blood progenitor cell transplantation (PBPCT) following high-dose melphalan alone or high-dose melphalan and total body irradiation. Peripheral blood progenitor cells (PBPCs) were mobilized with cyclophosphamide and granulocyte colony stimulating factor (G-CSF). CD34+ selection using the Cellpro Ceprate-SC system was performed in 22 cases with an adequate yield in 20. 10 patients failed to mobilize sufficient cells to permit selection and in four cases selection was not performed for other reasons. 16 patients therefore received unselected PBPC. Tumour cell contamination was evaluated by IgH gene fingerprinting (fpPCR). Harvested PBPC were fpPCR positive in 13/20 CD34+-selected cases and remained positive after selection in seven. Harvested PBPC were studied in 9/16 patients receiving unselected cells; fpPCR was positive in five and negative in four. There was no difference in event-free survival (EFS) between the CD34+-selected group and the unselected group (median 21 and 26 months, respectively, P=ns). The CD34+-selection process therefore reduced contamination but did not eliminate it completely, and in this small non-randomized study there was no apparent clinical benefit of CD34+ selection.

journal_name

Br J Haematol

authors

Gupta D,Bybee A,Cooke F,Giles C,Davis JG,McDonald C,Armitage SE,McGuigan D,Lyttelton MP,Kanfer EJ,Apperley JF,Samson D

doi

10.1046/j.1365-2141.1999.01133.x

subject

Has Abstract

pub_date

1999-01-01 00:00:00

pages

166-77

issue

1

eissn

0007-1048

issn

1365-2141

journal_volume

104

pub_type

临床试验,杂志文章
  • Bi-directional activation between mesenchymal stem cells and CLL B-cells: implication for CLL disease progression.

    abstract::It was hypothesized that contact between chronic lymphocytic leukaemia (CLL) B-cells and marrow stromal cells impact both cell types. To test this hypothesis, we utilized a long-term primary culture system from bone biopsies that reliably generates a mesenchymal stem cell (MSC). Co-culture of MSC with CLL B-cells prot...

    journal_title:British journal of haematology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2141.2009.07868.x

    authors: Ding W,Nowakowski GS,Knox TR,Boysen JC,Maas ML,Schwager SM,Wu W,Wellik LE,Dietz AB,Ghosh AK,Secreto CR,Medina KL,Shanafelt TD,Zent CS,Call TG,Kay NE

    更新日期:2009-11-01 00:00:00

  • Coagulation activation and hyperviscosity in infection.

    abstract::A serial study of coagulation activation and whole-blood viscosity was performed on 37 patients with local or systemic bacterial infection, malaria, or a viral infection. Thrombocytopenia, without consumption of coagulation factors, was the main feature of benign tertian malaria and viral infection, whereas in septica...

    journal_title:British journal of haematology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2141.1979.tb01155.x

    authors: Richardson SG,Matthews KB,Cruickshank JK,Geddes AM,Stuart J

    更新日期:1979-07-01 00:00:00

  • Immature dendritic cells in multiple myeloma are prone to osteoclast-like differentiation through interleukin-17A stimulation.

    abstract::Interleukin 17A (IL17A), a cytokine involved in allergy, inflammation and osteoclastogenesis, was investigated in multiple myeloma (MM) to assess its role in the osteoclast (OC)-like activity of marrow immature dendritic cells (iDCs). Comparing nine MM patients with control subjects affected by monoclonal gammopathy o...

    journal_title:British journal of haematology

    pub_type: 杂志文章

    doi:10.1111/bjh.12333

    authors: Tucci M,Stucci S,Savonarola A,Ciavarella S,Cafforio P,Dammacco F,Silvestris F

    更新日期:2013-06-01 00:00:00

  • Thalassaemia intermedia in Cyprus: the interaction of alpha and beta thalassaemia.

    abstract::Restriction endonuclease analysis has been performed on the alpha and beta globin gene clusters of 57 Cypriots homozygous for beta thalassaemia, 30 with the transfusion dependent form of the condition (thalassaemia major) and 27 who are less severely affected (thalassaemia intermedia). There was a significant differen...

    journal_title:British journal of haematology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2141.1983.tb02041.x

    authors: Wainscoat JS,Kanavakis E,Wood WG,Letsky EA,Huehns ER,Marsh GW,Higgs DR,Clegg JB,Weatherall DJ

    更新日期:1983-03-01 00:00:00

  • Rapid detection of the major Mediterranean beta-thalassaemia mutations by real-time polymerase chain reaction using fluorophore-labelled hybridization probes.

    abstract::We present a new method to detect the major beta-thalassaemia mutations of the Mediterranean countries (IVS I-1, IVS I-6, IVS I-110, CD-37 and CD-39). The procedure is based upon real-time polymerase chain reaction (PCR), using specific fluorescently labelled hybridization probes. The melting curves for each of the sp...

    journal_title:British journal of haematology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2141.2002.03823.x

    authors: Moreno I,Bolufer P,Perez ML,Barragán E,Sanz MA

    更新日期:2002-11-01 00:00:00

  • Two novel imatinib-responsive PDGFRA fusion genes in chronic eosinophilic leukaemia.

    abstract::We identified two patients with a t(2;4)(p24;q12) and a t(4;12)(q2?3;p1?2), respectively, in association with BCR-ABL and FIP1L1-PDGFRA negative chronic eosinophilic leukaemia. Molecular analysis revealed a novel STRN-PDGFRA fusion for the t(2;4) and ETV6-PDGFRA for the t(4;12). The fusions were confirmed by specific ...

    journal_title:British journal of haematology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2141.2007.06628.x

    authors: Curtis CE,Grand FH,Musto P,Clark A,Murphy J,Perla G,Minervini MM,Stewart J,Reiter A,Cross NC

    更新日期:2007-07-01 00:00:00

  • Circulating immune complexes involving the ABO system after platelet transfusion.

    abstract::It has been proposed that when ABO unmatched platelets are transfused circulating immune complexes (CIC) may be formed between the patient's soluble ABH antigens and the transfused antibodies. Platelets might then be destroyed by bystander mechanisms or by the binding of CIC to the Fc receptor or to C3 binding membran...

    journal_title:British journal of haematology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2141.1993.tb03349.x

    authors: Heal JM,Masel D,Rowe JM,Blumberg N

    更新日期:1993-11-01 00:00:00

  • Deletion of sequences flanking the t(9;22) breakpoint: a secondary genetic event associated with loss of cytogenetic response to interferon in a Philadelphia-positive chronic myeloid leukaemia patient.

    abstract::We present a Ph-positive chronic myeloid leukaemia patient who lost a complete cytogenetic response (CCR) of 23 months duration at the time of detection of a deletion, not previously observed, of chromosomes 9 and 22 sequences flanking the translocation breakpoint on the derivate 9 chromosome. To our knowledge, this i...

    journal_title:British journal of haematology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2141.2002.03432.x

    authors: Arranz E,Gil-Fernández JJ,Ramiro S,Blas C,Renedo M,Alegre A,Escudero A,Fernández-Rañada JM

    更新日期:2002-06-01 00:00:00

  • The anti-mitotic agents PTC-028 and PTC596 display potent activity in pre-clinical models of multiple myeloma but challenge the role of BMI-1 as an essential tumour gene.

    abstract::Future progress in the treatment of multiple myeloma (MM) requires both the characterisation of key drivers of the disease and novel, innovative approaches to tackle these vulnerabilities. The present study focussed on the pre-clinical evaluation of a novel drug class, BMI-1 modulators, in MM. We demonstrate potent ac...

    journal_title:British journal of haematology

    pub_type: 杂志文章

    doi:10.1111/bjh.16595

    authors: Bolomsky A,Muller J,Stangelberger K,Lejeune M,Duray E,Breid H,Vrancken L,Pfeiffer C,Hübl W,Willheim M,Weetall M,Branstrom A,Zojer N,Caers J,Ludwig H

    更新日期:2020-09-01 00:00:00

  • Normal individuals with high Hb A2 levels.

    abstract::Increased haemoglobin (Hb) A2 levels associated with reduced mean corpuscular volume (MCV) and Hb content per cell (MCH) are the most typical features of heterozygous beta thalassaemia. However, double heterozygotes for alpha and beta thalassaemia may have normal MCV and MCH but Hb A2 always in the carrier range. In t...

    journal_title:British journal of haematology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2141.1993.tb03042.x

    authors: Gasperini D,Cao A,Paderi L,Barella S,Paglietti E,Perseu L,Loi D,Galanello R

    更新日期:1993-05-01 00:00:00

  • Weekly versus twice weekly bortezomib given in conjunction with rituximab, in patients with recurrent follicular lymphoma, mantle cell lymphoma and Waldenström macroglobulinaemia.

    abstract::The combination of bortezomib and rituximab was evaluated in patients with mantle cell lymphoma (MCL), follicular lymphoma (FL) and Waldenström macroglobulinaemia (WM), in a Phase I and later, a randomized Phase II study. In the randomized study, 42 patients with recurrent/refractory disease received either: bortezomi...

    journal_title:British journal of haematology

    pub_type: 杂志文章,多中心研究,随机对照试验

    doi:10.1111/j.1365-2141.2010.08340.x

    authors: Agathocleous A,Rohatiner A,Rule S,Hunter H,Kerr JP,Neeson SM,Matthews J,Strauss S,Montoto S,Johnson P,Radford J,Lister A

    更新日期:2010-11-01 00:00:00

  • Single amino acid mutation of Fc gamma receptor is associated with the development of heparin-induced thrombocytopenia.

    abstract::Heparin-induced thrombocytopenia (HIT) is mediated by a heparin-dependent antibody/platelet factor 4/heparin complex binding to platelets via the Fc gamma receptor (type IIA). A single base polymorphism at position 131 of Fc gamma RIIA changes the native arginine to histidine. In the presence of murine monoclonal IgG1...

    journal_title:British journal of haematology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2141.1995.tb05383.x

    authors: Burgess JK,Lindeman R,Chesterman CN,Chong BH

    更新日期:1995-11-01 00:00:00

  • Australian Leukaemia Study Group myeloma II: a randomized trial of intensive combination chemotherapy with or without interferon in patients with myeloma.

    abstract::The Australian Leukaemia Study Group has performed a randomized trial of interferon alpha-2A (Roferon-A) as a co-induction agent together with intensive combination chemotherapy and as maintenance following completion of 12 cycles of induction treatment. When used as a co-induction agent, interferon-alpha did not impr...

    journal_title:British journal of haematology

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:10.1046/j.1365-2141.1997.9942643.x

    authors: Joshua DE,Penny R,Matthews JP,Laidlaw CR,Gibson J,Bradstock K,Wolf M,Goldstein D

    更新日期:1997-04-01 00:00:00

  • Monocyte tissue factor-like activity in post myocardial infarction patients.

    abstract::It is widely recognized that thrombosis is the major event in the evolution of stable vascular disease to unstable ischaemic syndromes including myocardial infarction and stroke. The purpose of this case-control study was to establish clinical and laboratory data on the possible relationship between specific component...

    journal_title:British journal of haematology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2141.1998.00804.x

    authors: Freeburn JC,Wallace JM,Strain JJ,Sinnamon DG,Craig BM,Johnson D,Gilmore WS

    更新日期:1998-07-01 00:00:00

  • Serial changes in coagulation and viscosity during sickle-cell crisis.

    abstract::Coagulation activity and whole-blood viscosity were measured in the steady state, and serially during painful crisis, in eight patients with sickle-cell anaemia. Platelet and coagulation activation occurred in the steady state and became more pronounced early in crisis. Whole-blood viscosity increased during crisis in...

    journal_title:British journal of haematology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2141.1979.tb03685.x

    authors: Richardson SG,Matthews KB,Stuart J,Geddes AM,Wilcox RM

    更新日期:1979-01-01 00:00:00

  • Gender, race and diet affect platelet function tests in normal subjects, contributing to a high rate of abnormal results.

    abstract::To assess sources of variability in platelet function tests in normal subjects, 64 healthy young adults were tested on 2-6 occasions at 2 week intervals using four methods: platelet aggregation (AGG) in platelet-rich plasma (PRP) in the Bio/Data PAP-4 Aggregometer (BD) and Chrono-Log Lumi-Aggregometer (CL); and AGG in...

    journal_title:British journal of haematology

    pub_type: 杂志文章

    doi:10.1111/bjh.12827

    authors: Miller CH,Rice AS,Garrett K,Stein SF

    更新日期:2014-06-01 00:00:00

  • Effects of oxidative stress on red blood cell rheology in sickle cell patients.

    abstract::Sickle cell anaemia (SS) and sickle cell-haemoglobin C disease (SC) patients exhibit severe red blood cell (RBC) rheological alterations involved in the development of several complications. The contribution of oxidative stress in these haemorheological abnormalities is still unknown. We compared RBC reactive oxygen s...

    journal_title:British journal of haematology

    pub_type: 杂志文章

    doi:10.1111/bjh.12912

    authors: Hierso R,Waltz X,Mora P,Romana M,Lemonne N,Connes P,Hardy-Dessources MD

    更新日期:2014-08-01 00:00:00

  • N-acetylglucosamine kinase and N-acetylglucosamine 6-phosphate deacetylase in normal human erythrocytes and Plasmodium falciparum.

    abstract::The major pathways of glucose metabolism in the malaria parasite, Plasmodium falciparum, have now been elucidated, and the structures and properties of parasite-specific enzymes are presently being investigated. Little is known, however, about the enzymes catalysing monosaccharide interconversions in the parasite. In ...

    journal_title:British journal of haematology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2141.1996.d01-1955.x

    authors: Weidanz JA,Campbell P,Moore D,DeLucas LJ,Rodén L,Thompson JN,Vezza AC

    更新日期:1996-12-01 00:00:00

  • The CDKN2 gene alterations in various types of adult T-cell leukaemia.

    abstract::The CDKN2 gene, encoding the cyclin-dependent kinase-4 inhibitor p16, is a putative tumour-suppressor gene because it is frequently altered in many malignant tumours. We analysed the CDKN2 gene in 44 cases of adult T-cell leukaemia (ATL) by Southern blot analysis, polymerase chain reaction-mediated single-strand confo...

    journal_title:British journal of haematology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2141.1996.d01-1841.x

    authors: Uchida T,Kinoshita T,Watanabe T,Nagai H,Murate T,Saito H,Hotta T

    更新日期:1996-09-01 00:00:00

  • Transient familial haemophagocytic lymphohistiocytosis reactivation post-CD34 haematopoietic stem cell transplantation.

    abstract::Familial haemophagocytic lymphohistiocytosis (FHLH) is a genetic disorder caused by defective lymphocyte cytotoxicity, resulting in impaired lymphocyte homeostasis and macrophage infiltration of solid tissues and bone marrow, with extensive haemophagocytosis. It is invariably fatal unless treated by allogeneic haemato...

    journal_title:British journal of haematology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2141.2005.05615.x

    authors: Almousa H,Ouachée-Chardin M,Picard C,Radford-Weiss I,Caillat-Zucman S,Cavazzana-Calvo M,Blanche S,de Saint Basile G,Le Deist F,Fischer A

    更新日期:2005-08-01 00:00:00

  • Platelet associated complement components (PAC3c and PAC3d) in patients with autoimmune thrombocytopenia.

    abstract::Platelet associated C3c and C3d (PAC3c and PAC3d) were quantitated by enzyme linked assay in 105 patients with idiopathic autoimmune thrombocytopenia (AITP) in whom elevated platelet associated immunoglobulins (IgG and IgM) had previously been documented. Increased levels of complement components were demonstrated in ...

    journal_title:British journal of haematology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2141.1985.tb07384.x

    authors: Hegde UM,Bowes A,Roter BL

    更新日期:1985-05-01 00:00:00

  • Targeting malignant B-cell lymphoma with a humanized anti-CD22 scFv-angiogenin immunoenzyme.

    abstract::We report on the generation and functional characterization of a humanized immunoenzyme comprising a stable humanized single chain Fv (scFv) with grafted specificity of the anti-CD22 murine monoclonal antibody RFB4 and the human ribonuclease angiogenin (ANG). The fusion protein produced from transiently transfected ma...

    journal_title:British journal of haematology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2141.2005.05356.x

    authors: Krauss J,Arndt MA,Vu BK,Newton DL,Rybak SM

    更新日期:2005-03-01 00:00:00

  • A randomized, double-blind trial of pegfilgrastim versus filgrastim for the management of neutropenia during CHASE(R) chemotherapy for malignant lymphoma.

    abstract::Pegfilgrastim is a pegylated form of the granulocyte-colony stimulating factor, filgrastim. Herein, we report the results of a multicentre, randomized, double-blind phase III trial comparing the efficacy and safety of pegfilgrastim with filgrastim in patients with malignant lymphoma. Patients were randomized to receiv...

    journal_title:British journal of haematology

    pub_type: 杂志文章,多中心研究,随机对照试验

    doi:10.1111/bjh.14088

    authors: Kubo K,Miyazaki Y,Murayama T,Shimazaki R,Usui N,Urabe A,Hotta T,Tamura K

    更新日期:2016-08-01 00:00:00

  • Short-term phytohaemagglutinin-activated mononuclear cells induce endothelial progenitor cells from cord blood CD34+ cells.

    abstract::Endothelial progenitor cells (EPCs) were recently demonstrated to exist in human cord blood. Phytohaemagglutinin (PHA), a potent mitogen for mononuclear cells was used to induce EPCs from unsorted cord blood mononuclear cells (CBMCs). Adherent cells in clusters appeared approximately 24 h after CBMCs were cultured in ...

    journal_title:British journal of haematology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2141.2001.02694.x

    authors: Kang HJ,Kim SC,Kim YJ,Kim CW,Kim JG,Ahn HS,Park SI,Jung MH,Choi BC,Kimm K

    更新日期:2001-06-01 00:00:00

  • Chimaeric anti-CD20 monoclonal antibody (rituximab) in post-transplant B-lymphoproliferative disorder following stem cell transplantation in children.

    abstract::Post-transplant lymphoproliferative disorder (PTLD) after haemopoietic stem cell transplantation is a serious complication that occurs in 8-22% of patients with high-risk factors. We retrospectively investigated tolerance and efficacy of humanized anti-CD20 monoclonal antibody (rituximab) as first-line treatment in 12...

    journal_title:British journal of haematology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2141.2001.03041.x

    authors: Faye A,Quartier P,Reguerre Y,Lutz P,Carret AS,Dehée A,Rohrlich P,Peuchmaur M,Matthieu-Boué A,Fischer A,Vilmer E

    更新日期:2001-10-01 00:00:00

  • Nicotinamide (vitamin B3) increases the polyploidisation and proplatelet formation of cultured primary human megakaryocytes.

    abstract::Megakaryocytic (Mk) cell maturation involves polyploidisation, and the number of platelets produced increases with Mk DNA content. Ploidy levels in cultured human MK cells are much lower than those observed in vivo. This study demonstrated that adding the water-soluble vitamin nicotinamide (NIC) to mobilised periphera...

    journal_title:British journal of haematology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2141.2006.06341.x

    authors: Giammona LM,Fuhrken PG,Papoutsakis ET,Miller WM

    更新日期:2006-11-01 00:00:00

  • Bone resorption is increased in young adults with thalassaemia major.

    abstract::Bone disease in patients with thalassaemia major is a multifactorial and still poorly understood process. The present study evaluated 45 thalassaemic patients using dual X-ray absorptiometry at three sites (lumbar spine, head of femur and forearm) to assess bone mineral density, in parallel with a series of biochemica...

    journal_title:British journal of haematology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2141.2001.02549.x

    authors: Voskaridou E,Kyrtsonis MC,Terpos E,Skordili M,Theodoropoulos I,Bergele A,Diamanti E,Kalovidouris A,Loutradi A,Loukopoulos D

    更新日期:2001-01-01 00:00:00

  • Factor IX gene haplotypes in Brazilian blacks and characterization of unusual DdeI alleles.

    abstract::Analysis of factor IX gene polymorphisms is considered the best approach for prenatal diagnosis and carrier detection of haemophilia B when the identification of the gene mutation is not possible. Studies involving factor IX gene polymorphisms in Black populations are scarce and essentially restricted to the North-Ame...

    journal_title:British journal of haematology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2141.1994.tb06739.x

    authors: Figueiredo MS,Bowen DJ,Silva Júnior WA,Zago MA

    更新日期:1994-08-01 00:00:00

  • Chronic myeloproliferative disorders: improved platelet aggregation following venesection.

    abstract::Venesection of 10% of whole blood volume or plateletpheresis was performed in nine patients with chronic myeloproliferative disorders and in five normal control subjects. Before venesection, the patients showed impaired platelet aggregation in 33% of tests, most often in response to stimulation with 9 mumol adrenaline...

    journal_title:British journal of haematology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2141.1978.tb03629.x

    authors: Boughton BJ

    更新日期:1978-08-01 00:00:00

  • Recent advances in the pathogenesis and treatment of juvenile myelomonocytic leukaemia.

    abstract::Myeloid neoplasms derive from the pathological clonal expansion of an abnormal stem cell and span a diverse spectrum of phenotypes including acute myeloid leukaemia (AML), myeloproliferative neoplasms (MPN) and myelodysplastic syndromes (MDS). Expansion of myeloid blasts with suppression of normal haematopoiesis is th...

    journal_title:British journal of haematology

    pub_type: 杂志文章,评审

    doi:10.1111/j.1365-2141.2010.08525.x

    authors: Loh ML

    更新日期:2011-03-01 00:00:00